Gene description for Osbp
Gene name oxysterol binding protein
Gene symbol Osbp
Other names/aliases 1110018F06Rik
AW559088
mKIAA4220
Species Mus musculus
 Database cross references - Osbp
ExoCarta ExoCarta_76303
Vesiclepedia VP_76303
Entrez Gene 76303
UniProt Q3B7Z2  
 Osbp identified in sEVs derived from the following tissue/cell type
Colon cancer cells 37309723    
T-cell lymphoma cells 37309723    
 Gene ontology annotations for Osbp
Molecular Function
    protein binding GO:0005515 IPI
    protein domain specific binding GO:0019904 IEA
    protein domain specific binding GO:0019904 ISO
    sterol binding GO:0032934 IBA
    phosphatidylinositol-4-phosphate binding GO:0070273 ISO
    phosphatidylinositol-4-phosphate binding GO:0070273 ISS
    sterol transfer activity GO:0120015 ISS
Biological Process
    sphingomyelin biosynthetic process GO:0006686 ISO
    phospholipid transport GO:0015914 ISO
    sterol transport GO:0015918 ISO
    sterol transport GO:0015918 ISS
    intracellular cholesterol transport GO:0032367 ISO
    intracellular cholesterol transport GO:0032367 ISS
    ceramide transport GO:0035627 ISO
    positive regulation of insulin secretion involved in cellular response to glucose stimulus GO:0035774 IEA
    positive regulation of insulin secretion involved in cellular response to glucose stimulus GO:0035774 ISO
    positive regulation of tyrosine phosphorylation of STAT protein GO:0042531 ISO
    positive regulation of secretory granule organization GO:1904411 IEA
    positive regulation of secretory granule organization GO:1904411 ISO
Subcellular Localization
    Golgi membrane GO:0000139 ISO
    Golgi membrane GO:0000139 ISS
    nucleoplasm GO:0005654 IEA
    nucleoplasm GO:0005654 ISO
    nucleolus GO:0005730 IEA
    nucleolus GO:0005730 ISO
    cytoplasm GO:0005737 ISO
    endoplasmic reticulum membrane GO:0005789 ISO
    Golgi apparatus GO:0005794 ISO
    trans-Golgi network GO:0005802 ISO
    trans-Golgi network GO:0005802 ISS
    cytosol GO:0005829 IBA
    cytosol GO:0005829 ISO
    cytosol GO:0005829 ISS
    plasma membrane GO:0005886 IBA
    membrane GO:0016020 ISO
    cell junction GO:0030054 IEA
    cell junction GO:0030054 ISO
    perinuclear region of cytoplasm GO:0048471 ISO
    perinuclear region of cytoplasm GO:0048471 ISS
    perinuclear endoplasmic reticulum GO:0097038 IBA
 Experiment description of studies that identified Osbp in sEVs
1
Experiment ID 907
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample Colon cancer cells
Sample name C26
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
2
Experiment ID 908
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample T-cell lymphoma cells
Sample name EL4
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for Osbp
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 Ttc1  
Co-fractionation Mus musculus
2 Ptges3 56351
Co-fractionation Mus musculus
3 Swap70  
Co-fractionation Mus musculus
4 Tmem173  
Proximity Label-MS Mus musculus
5 Vapa 30960
Co-fractionation Mus musculus
View the network image/svg+xml



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