Gene description for Vapa
Gene name vesicle-associated membrane protein, associated protein A
Gene symbol Vapa
Other names/aliases 33kDa
VAP33
Species Mus musculus
 Database cross references - Vapa
ExoCarta ExoCarta_30960
Entrez Gene 30960
UniProt Q9WV55  
 Vapa identified in exosomes derived from the following tissue/cell type
Mast cells 17486113    
 Gene ontology annotations for Vapa
Molecular Function
    protein heterodimerization activity GO:0046982 ISO
    microtubule binding GO:0008017 ISO
    signal transducer activity GO:0004871 ISO
    FFAT motif binding GO:0033149 ISO
    protein domain specific binding GO:0019904 ISO
Biological Process
    negative regulation by host of viral genome replication GO:0044828 ISO
    positive regulation of I-kappaB kinase/NF-kappaB signaling GO:0043123 ISO
    protein localization to endoplasmic reticulum GO:0070972 ISO
    cell death GO:0008219 ISO
    positive regulation by host of viral genome replication GO:0044829 ISO
    COPII-coated vesicle budding GO:0090114 ISO
    ER to Golgi vesicle-mediated transport GO:0006888 ISO
    signal transduction GO:0007165 ISO
    neuron projection development GO:0031175 ISO
    positive regulation by host of viral release from host cell GO:0044791 ISO
Subcellular Localization
    Golgi membrane GO:0000139 ISO
    membrane GO:0016020 ISO
    endoplasmic reticulum GO:0005783 ISO
    perinuclear region of cytoplasm GO:0048471 ISO
    vesicle GO:0031982 ISO
    integral component of membrane GO:0016021 IEA
    bicellular tight junction GO:0005923 ISO
    endoplasmic reticulum membrane GO:0005789 IEA
    microtubule cytoskeleton GO:0015630 IDA
    plasma membrane GO:0005886 ISO
 Experiment description of studies that identified Vapa in exosomes
1
Experiment ID 15
ISEV standards
EM
EV Biophysical techniques
EV Cytosolic markers
CD63
EV Membrane markers
EV Negative markers
EV Particle analysis
Identified molecule mrna
Identification method Microarray
PubMed ID 17486113    
Organism Mus musculus
Homo sapiens
Experiment description Exosome-mediated transfer of mRNAs and microRNAs is a novel mechanism of genetic exchange between cells.
Authors Valadi H, Ekstrom K, Bossios A, Sjostrand M, Lee JJ, Lotvall JO
Journal name NCB
Publication year 2007
Sample Mast cells
Sample name MC9
Bone marrow-derived mast cells
HMC-1
Isolation/purification methods Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.11-1.21 g/mL
Molecules identified in the study Protein
mRNA
miRNA
Methods used in the study Mass spectrometry [MALDI TOF]
Western blotting
Microarray
miRCURY LNA Array
 Protein-protein interactions for Vapa
  Protein Interactor ExoCarta ID Identification method PubMed Species
No interactions are found.
 Pathways in which Vapa is involved
PathwayEvidenceSource
Sphingolipid de novo biosynthesis IEA Reactome





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