Gene description for SMURF2
Gene name SMAD specific E3 ubiquitin protein ligase 2
Gene symbol SMURF2
Other names/aliases -
Species Homo sapiens
 Database cross references - SMURF2
ExoCarta ExoCarta_64750
Vesiclepedia VP_64750
Entrez Gene 64750
HGNC 16809
MIM 605532
UniProt Q9HAU4  
 SMURF2 identified in exosomes derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Gastric cancer cells 33991177    
Gastric cancer cells 33991177    
Gastric cancer cells 33991177    
Mesenchymal stem cells 36408942    
 Gene ontology annotations for SMURF2
Molecular Function
    protein binding GO:0005515 IPI
    transforming growth factor beta receptor binding GO:0005160 IEA
    ubiquitin-protein transferase activity GO:0004842 TAS
    ubiquitin protein ligase activity GO:0061630 TAS
    identical protein binding GO:0042802 IPI
    SMAD binding GO:0046332 IPI
    ligase activity GO:0016874 IEA
Biological Process
    regulation of transforming growth factor beta receptor signaling pathway GO:0017015 NAS
    transcription initiation from RNA polymerase II promoter GO:0006367 TAS
    gene expression GO:0010467 TAS
    negative regulation of transcription from RNA polymerase II promoter GO:0000122 TAS
    positive regulation of canonical Wnt signaling pathway GO:0090263 TAS
    transcription, DNA-templated GO:0006351 TAS
    transforming growth factor beta receptor signaling pathway GO:0007179 TAS
    negative regulation of transforming growth factor beta receptor signaling pathway GO:0030512 TAS
    ubiquitin-dependent SMAD protein catabolic process GO:0030579 IDA
    positive regulation of trophoblast cell migration GO:1901165 IDA
    ubiquitin-dependent protein catabolic process GO:0006511 IDA
    protein ubiquitination involved in ubiquitin-dependent protein catabolic process GO:0042787 IBA
    negative regulation of transcription, DNA-templated GO:0045892 NAS
    BMP signaling pathway GO:0030509 TAS
Subcellular Localization
    nucleus GO:0005634 NAS
    nucleoplasm GO:0005654 TAS
    cytoplasm GO:0005737 IBA
    plasma membrane GO:0005886 IEA
    cytosol GO:0005829 TAS
    ubiquitin ligase complex GO:0000151 IDA
    membrane raft GO:0045121 IEA
 Experiment description of studies that identified SMURF2 in exosomes
1
Experiment ID 489
MISEV standards
✔
EM
EV Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
EV Enriched markers
✔
Canx
EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ.
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
✔
EM
EV Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
EV Enriched markers
✔
Canx
EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ.
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
✔
EM
EV Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
EV Enriched markers
✔
Canx
EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ.
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
✔
EM
EV Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
EV Enriched markers
✔
Canx
EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ.
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
5
Experiment ID 363
MISEV standards
✘ EV Biophysical techniques
✔
TSG101|GAPDH|AQP1|CD151|CD81|CD82|CD9|EPCAM|FLOT1|FLOT2|ICAM1|ITGA2B|LAMP2|RAB35|RAB5A|RAB5B|SDCBP|TFRC|UCHL1
EV Enriched markers
✔
DCLK1
EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 33991177    
Organism Homo sapiens
Experiment description Cancer stem cell marker DCLK1 reprograms small extracellular vesicles toward migratory phenotype in gastric cancer cells
Authors Carli ALE, Afshar-Sterle S, Rai A, Fang H, O'Keefe R, Tse J, Ferguson FM, Gray NS, Ernst M, Greening DW, Buchert M.
Journal name Proteomics
Publication year 2021
Sample Gastric cancer cells
Sample name MKN1 - 100K pellet
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
6
Experiment ID 364
MISEV standards
✘ EV Biophysical techniques
✔
TSG101|GAPDH|AQP1|CD151|CD81|CD82|CD9|EPCAM|FLOT1|FLOT2|ICAM1|ITGA2B|LAMP2|RAB35|RAB5A|RAB5B|SDCBP|TFRC|UCHL1
EV Enriched markers
✔
DCLK1
EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 33991177    
Organism Homo sapiens
Experiment description Cancer stem cell marker DCLK1 reprograms small extracellular vesicles toward migratory phenotype in gastric cancer cells
Authors Carli ALE, Afshar-Sterle S, Rai A, Fang H, O'Keefe R, Tse J, Ferguson FM, Gray NS, Ernst M, Greening DW, Buchert M.
Journal name Proteomics
Publication year 2021
Sample Gastric cancer cells
Sample name MKN1 - 100K pellet
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
7
Experiment ID 365
MISEV standards
✘ EV Biophysical techniques
✔
TSG101|GAPDH|AQP1|CD151|CD81|CD82|CD9|EPCAM|FLOT1|FLOT2|ICAM1|ITGA2B|LAMP2|RAB35|RAB5A|RAB5B|SDCBP|TFRC|UCHL1
EV Enriched markers
✔
DCLK1
EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 33991177    
Organism Homo sapiens
Experiment description Cancer stem cell marker DCLK1 reprograms small extracellular vesicles toward migratory phenotype in gastric cancer cells
Authors Carli ALE, Afshar-Sterle S, Rai A, Fang H, O'Keefe R, Tse J, Ferguson FM, Gray NS, Ernst M, Greening DW, Buchert M.
Journal name Proteomics
Publication year 2021
Sample Gastric cancer cells
Sample name MKN1 - 100K pellet
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
8
Experiment ID 488
MISEV standards
✔
EM
EV Biophysical techniques
✔
CD9|CD81|CD63|GAPDH|SDCBP|LAMP1|TFRC|UCHL1|FLOT2|LAMP2|FLOT1|ICAM1|RAB5B|CD151|RAB35|TSG101|RAB5A|CD82
EV Enriched markers
✔
CANX
EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Homo sapiens
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ.
Journal name Proteomics
Publication year 2023
Sample Mesenchymal stem cells
Sample name UCMSC
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
 Protein-protein interactions for SMURF2
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 Rab22a 19334
Affinity Capture-Luminescence Mus musculus
2 Arhgef1 16801
Affinity Capture-Luminescence Mus musculus
3 Smad7  
Affinity Capture-Luminescence Mus musculus
4 SKIL  
Affinity Capture-Western Homo sapiens
5 TGFBR2 7048
Affinity Capture-Western Homo sapiens
6 SMAD7  
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
7 Sfrs4  
Affinity Capture-Luminescence Mus musculus
8 Nras 18176
Affinity Capture-Luminescence Mus musculus
9 Rab13 68328
Affinity Capture-Luminescence Mus musculus
10 Smad3  
Affinity Capture-Luminescence Mus musculus
11 Rab17  
Affinity Capture-Luminescence Mus musculus
12 Ash2l  
Affinity Capture-Luminescence Mus musculus
13 Smad2  
Affinity Capture-Luminescence Mus musculus
14 Anapc5 59008
Affinity Capture-Luminescence Mus musculus
15 Rac1 19353
Affinity Capture-Luminescence Mus musculus
16 Irf3  
Affinity Capture-Luminescence Mus musculus
17 Rnf14  
Affinity Capture-Luminescence Mus musculus
18 Nkiras1  
Affinity Capture-Luminescence Mus musculus
19 Smap1  
Affinity Capture-Luminescence Mus musculus
20 Fkbp4 14228
Affinity Capture-Luminescence Mus musculus
21 Stat1 20846
Affinity Capture-Luminescence Mus musculus
22 Rras2 66922
Affinity Capture-Luminescence Mus musculus
23 Nola1  
Affinity Capture-Luminescence Mus musculus
24 TGFBR1 7046
Affinity Capture-Western Homo sapiens
25 SMAD1 4086
Two-hybrid Homo sapiens
Reconstituted Complex Homo sapiens
26 Epha1  
Affinity Capture-Luminescence Mus musculus
27 Ube2g1  
Affinity Capture-Luminescence Mus musculus
28 Rasd2  
Affinity Capture-Luminescence Mus musculus
29 Smad6  
Affinity Capture-Luminescence Mus musculus
30 Gng2 14702
Affinity Capture-Luminescence Mus musculus
31 Icsbp1  
Affinity Capture-Luminescence Mus musculus
32 SMAD3 4088
Two-hybrid Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
33 Rhod  
Affinity Capture-Luminescence Mus musculus
34 Rnf2  
Affinity Capture-Luminescence Mus musculus
35 SMAD2 4087
Two-hybrid Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
36 STAMBP 10617
Affinity Capture-Western Homo sapiens
37 Nek6  
Affinity Capture-Luminescence Mus musculus
38 Rab14 68365
Affinity Capture-Luminescence Mus musculus
39 Smad1  
Affinity Capture-Luminescence Mus musculus
40 Ing1l  
Affinity Capture-Luminescence Mus musculus
41 Ppid 67738
Affinity Capture-Luminescence Mus musculus
42 Hdgfrp3  
Affinity Capture-Luminescence Mus musculus
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