Gene description for Nras
Gene name
neuroblastoma ras oncogene
Gene symbol
Nras
Other names/aliases
AV095280 N-ras
Species
Mus musculus
Database cross references - Nras
ExoCarta
ExoCarta_18176
Entrez Gene
18176
Nras identified in exosomes derived from the following tissue/cell type
Embryonic fibroblasts
18494037
Fibroblasts
23260141
Mast cells
17486113
Gene ontology annotations for Nras
Experiment description of studies that identified Nras in exosomes
1
Experiment ID
73
ISEV standards
✔
EM
EV Biophysical techniques
✔
HSP70|HSP90
EV Cytosolic markers
✘
EV Membrane markers
✘
EV Negative markers
✘
EV Particle analysis
Identified molecule
protein
Identification method
Mass spectrometry
PubMed ID
18494037
Organism
Mus musculus
Experiment description
Difference gel electrophoresis analysis of Ras-transformed fibroblast cell-derived exosomes.
Authors
Ji H, Erfani N, Tauro BJ, Kapp EA, Zhu HJ, Moritz RL, Lim JW, Simpson RJ
Journal name
ELEC
Publication year
2008
Sample
Embryonic fibroblasts
Sample name
NIH3T3
Isolation/purification methods
Differential centrifugation
Flotation density
-
Molecules identified in the study
Protein
Methods used in the study
Mass spectrometry [LCQ DECA] Western blotting
2
Experiment ID
210
ISEV standards
✔
EM
EV Biophysical techniques
✘
EV Cytosolic markers
✔
CD81|FLOT1
EV Membrane markers
✘
EV Negative markers
✘
EV Particle analysis
Identified molecule
protein
Identification method
Mass spectrometry
PubMed ID
23260141
Organism
Mus musculus
Experiment description
Exosomes Mediate Stromal Mobilization of Autocrine Wnt-PCP Signaling in Breast Cancer Cell Migration.
Authors
Luga V, Zhang L, Viloria-Petit AM, Ogunjimi AA, Inanlou MR, Chiu E, Buchanan M, Hosein AN, Basik M, Wrana JL.
Journal name
Cell
Publication year
2012
Sample
Fibroblasts
Sample name
Normal-Fibroblasts (L cells)
Isolation/purification methods
Differential centrifugation Ultracentrifugation
Flotation density
-
Molecules identified in the study
Protein
Methods used in the study
Mass spectrometry
3
Experiment ID
15
ISEV standards
✔
EM
EV Biophysical techniques
✘
EV Cytosolic markers
✔
CD63
EV Membrane markers
✘
EV Negative markers
✘
EV Particle analysis
Identified molecule
mrna
Identification method
Microarray
PubMed ID
17486113
Organism
Mus musculus Homo sapiens
Experiment description
Exosome-mediated transfer of mRNAs and microRNAs is a novel mechanism of genetic exchange between cells.
Authors
Valadi H, Ekstrom K, Bossios A, Sjostrand M, Lee JJ, Lotvall JO
Journal name
NCB
Publication year
2007
Sample
Mast cells
Sample name
MC9 Bone marrow-derived mast cells HMC-1
Isolation/purification methods
Filtration Ultracentrifugation Sucrose density gradient
Flotation density
1.11-1.21 g/mL
Molecules identified in the study
Protein mRNA miRNA
Methods used in the study
Mass spectrometry [MALDI TOF] Western blotting Microarray miRCURY LNA Array
Protein-protein interactions for Nras
Protein Interactor
ExoCarta ID
Identification method
PubMed
Species
1
SMURF2
Affinity Capture-Luminescence
Homo sapiens
2
SMAD4
Affinity Capture-Luminescence
Homo sapiens
3
ACVR1
90
Affinity Capture-Luminescence
Homo sapiens
View the network
image/svg+xml
Pathways in which Nras is involved
No pathways found