Gene description for Pa2g4
Gene name proliferation-associated 2G4
Gene symbol Pa2g4
Other names/aliases 38kDa
AA672939
Ebp1
Plfap
Species Mus musculus
 Database cross references - Pa2g4
ExoCarta ExoCarta_18813
Vesiclepedia VP_18813
Entrez Gene 18813
UniProt P50580  
 Pa2g4 identified in exosomes derived from the following tissue/cell type
Colon cancer cells 37309723    
Neural stem cells 25242146    
T-cell lymphoma cells 37309723    
 Gene ontology annotations for Pa2g4
Molecular Function
    sequence-specific DNA binding transcription factor activity GO:0003700 ISA
    ubiquitin protein ligase binding GO:0031625 ISO
    DNA binding GO:0003677 ISA
    RNA binding GO:0003723 IEA
    poly(A) RNA binding GO:0044822 ISO
Biological Process
    negative regulation of transcription, DNA-templated GO:0045892 ISA
    positive regulation of cell differentiation GO:0045597 ISO
    negative regulation of apoptotic process GO:0043066 ISO
    transcription, DNA-templated GO:0006351 IEA
    regulation of translation GO:0006417 IEA
    regulation of transcription, DNA-templated GO:0006355 IEA
    rRNA processing GO:0006364 IEA
Subcellular Localization
    extracellular exosome GO:0070062 ISO
    membrane GO:0016020 ISO
    nucleoplasm GO:0005654 ISO
    cytoplasm GO:0005737 ISO
    ribonucleoprotein complex GO:0030529 ISO
    nucleus GO:0005634 ISO
    nucleolus GO:0005730 ISO
 Experiment description of studies that identified Pa2g4 in exosomes
1
Experiment ID 907
MISEV standards
EM
EV Biophysical techniques
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
EV Enriched markers
HSPA5
EV Negative markers
NTA
EV Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S.
Journal name Proteomics
Publication year 2023
Sample Colon cancer cells
Sample name C26
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
2
Experiment ID 264
MISEV standards
EV Biophysical techniques
CD63|CD9
EV Enriched markers
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25242146    
Organism Mus musculus
Experiment description Extracellular Vesicles from Neural Stem Cells Transfer IFN-γ via Ifngr1 to Activate Stat1 Signaling in Target Cells
Authors Chiara Cossetti, Nunzio Iraci, Tim R. Mercer, Tommaso Leonardi, Emanuele Alpi, Denise Drago, Clara Alfaro-Cervello, Harpreet K. Saini, Matthew P. Davis, Julia Schaeffer, Beatriz Vega, Matilde Stefanini, CongJian Zhao, Werner Muller, Jose Manuel Garcia-Verdugo, Suresh Mathivanan, Angela Bachi, Anton J. Enright, John S. Mattick, Stefano Pluchino
Journal name Molecular Cell
Publication year 2014
Sample Neural stem cells
Sample name NPCs - Th2 treated
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.20 g/mL
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
3
Experiment ID 908
MISEV standards
EM
EV Biophysical techniques
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
EV Enriched markers
HSPA5
EV Negative markers
NTA
EV Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S.
Journal name Proteomics
Publication year 2023
Sample T-cell lymphoma cells
Sample name EL4
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for Pa2g4
  Protein Interactor ExoCarta ID Identification method PubMed Species
No interactions are found.
 Pathways in which Pa2g4 is involved
No pathways found





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