Gene description for Pa2g4
Gene name proliferation-associated 2G4
Gene symbol Pa2g4
Other names/aliases 38kDa
AA672939
Ebp1
Plfap
Species Mus musculus
 Database cross references - Pa2g4
ExoCarta ExoCarta_18813
Vesiclepedia VP_18813
Entrez Gene 18813
UniProt P50580  
 Pa2g4 identified in sEVs derived from the following tissue/cell type
Colon cancer cells 37309723    
Neural stem cells 25242146    
T-cell lymphoma cells 37309723    
 Gene ontology annotations for Pa2g4
Molecular Function
    nucleic acid binding GO:0003676 ISO
    transcription corepressor activity GO:0003714 ISO
    RNA binding GO:0003723 IEA
    ubiquitin protein ligase binding GO:0031625 IEA
    ubiquitin protein ligase binding GO:0031625 ISO
Biological Process
    rRNA processing GO:0006364 IEA
    regulation of translation GO:0006417 IEA
    negative regulation of apoptotic process GO:0043066 ISO
    negative regulation of apoptotic process GO:0043066 ISS
    positive regulation of cell differentiation GO:0045597 ISO
    positive regulation of cell differentiation GO:0045597 ISS
    negative regulation of DNA-templated transcription GO:0045892 ISO
Subcellular Localization
    nucleus GO:0005634 ISO
    nucleolus GO:0005730 ISO
    nucleolus GO:0005730 ISS
    cytoplasm GO:0005737 ISO
    cytoplasm GO:0005737 ISS
    ribonucleoprotein complex GO:1990904 ISO
 Experiment description of studies that identified Pa2g4 in sEVs
1
Experiment ID 907
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample Colon cancer cells
Sample name C26
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
2
Experiment ID 264
MISEV standards
✘ Biophysical techniques
✔
CD63|CD9
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25242146    
Organism Mus musculus
Experiment description Extracellular Vesicles from Neural Stem Cells Transfer IFN-? via Ifngr1 to Activate Stat1 Signaling in Target Cells
Authors "Chiara Cossetti, Nunzio Iraci, Tim R. Mercer, Tommaso Leonardi, Emanuele Alpi, Denise Drago, Clara Alfaro-Cervello, Harpreet K. Saini, Matthew P. Davis, Julia Schaeffer, Beatriz Vega, Matilde Stefanini, CongJian Zhao, Werner Muller, Jose Manuel Garcia-Verdugo, Suresh Mathivanan, Angela Bachi, Anton J. Enright, John S. Mattick, Stefano Pluchino"
Journal name Molecular Cell
Publication year 2014
Sample Neural stem cells
Sample name NPCs - Th2 treated
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.20 g/mL
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
3
Experiment ID 908
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample T-cell lymphoma cells
Sample name EL4
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for Pa2g4
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 STUB1 10273
Affinity Capture-MS Homo sapiens
2 Elf5  
Affinity Capture-MS Mus musculus
3 Rplp2 67186
Co-fractionation Mus musculus
4 Pik3r1  
Affinity Capture-Western Mus musculus
Affinity Capture-Western Mus musculus
5 Nras 18176
Synthetic Lethality Mus musculus
6 Ubc  
Reconstituted Complex Mus musculus
7 Rpl27 19942
Co-fractionation Mus musculus
8 Tfe3  
Affinity Capture-MS Mus musculus
9 Rps6 20104
Co-fractionation Mus musculus
10 Rpl18a 76808
Co-fractionation Mus musculus
11 Rpl12 269261
Co-fractionation Mus musculus
12 Rpl18 19899
Co-fractionation Mus musculus
13 Rpl23 65019
Co-fractionation Mus musculus
14 HSPA4 3308
Affinity Capture-MS Homo sapiens
15 Rplp0 11837
Co-fractionation Mus musculus
16 Rpl23a 268449
Co-fractionation Mus musculus
17 Rpl30 19946
Co-fractionation Mus musculus
18 Rpl3 27367
Co-fractionation Mus musculus
19 Atg16l1  
Affinity Capture-MS Mus musculus
20 Rpl4 67891
Co-fractionation Mus musculus
21 Fancd2  
Affinity Capture-MS Mus musculus
22 Rpl8 26961
Co-fractionation Mus musculus
View the network image/svg+xml
 Pathways in which Pa2g4 is involved
PathwayEvidenceSource
Immune System IEA Reactome
Innate Immune System IEA Reactome
Neutrophil degranulation IEA Reactome





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