Gene description for Rps6
Gene name ribosomal protein S6
Gene symbol Rps6
Other names/aliases S6R
Species Mus musculus
 Database cross references - Rps6
ExoCarta ExoCarta_20104
Entrez Gene 20104
UniProt P62754  
 Rps6 identified in exosomes derived from the following tissue/cell type
Mast cells 17486113    
Pancreatic cells 19351151    
 Gene ontology annotations for Rps6
Molecular Function
    protein kinase binding GO:0019901 ISO
    structural constituent of ribosome GO:0003735 ISO
    protein binding GO:0005515 IPI
    poly(A) RNA binding GO:0044822 ISO
Biological Process
    translation GO:0006412 IEA
    gastrulation GO:0007369 IMP
    mitotic cell cycle checkpoint GO:0007093 IMP
    negative regulation of apoptotic process GO:0043066 IMP
    T cell differentiation in thymus GO:0033077 IMP
    mitotic nuclear division GO:0007067 IMP
    placenta development GO:0001890 IMP
    TOR signaling GO:0031929 ISO
    oogenesis stage GO:0022605 IMP
    G1/S transition of mitotic cell cycle GO:0000082 IMP
    erythrocyte development GO:0048821 IMP
    T cell proliferation involved in immune response GO:0002309 IMP
    activation-induced cell death of T cells GO:0006924 IMP
    positive regulation of apoptotic process GO:0043065 ISO
    ribosomal small subunit biogenesis GO:0042274 ISO
    rRNA processing GO:0006364 ISO
    glucose homeostasis GO:0042593 IDA
Subcellular Localization
    nucleus GO:0005634 ISO
    nucleolus GO:0005730 ISO
    intracellular GO:0005622 IEA
    ribonucleoprotein complex GO:0030529 ISO
    cell body GO:0044297 ISO
    dendrite GO:0030425 ISO
    ribosome GO:0005840 IDA
    perinuclear region of cytoplasm GO:0048471 IDA
    polysome GO:0005844 IDA
    membrane GO:0016020 ISO
    cytoplasm GO:0005737 ISO
    small ribosomal subunit GO:0015935 ISO
    cytosolic small ribosomal subunit GO:0022627 ISO
    cytoplasmic ribonucleoprotein granule GO:0036464 ISO
 Experiment description of studies that identified Rps6 in exosomes
1
Experiment ID 15
ISEV standards
EM
EV Biophysical techniques
EV Cytosolic markers
CD63
EV Membrane markers
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 17486113    
Organism Mus musculus
Homo sapiens
Experiment description Exosome-mediated transfer of mRNAs and microRNAs is a novel mechanism of genetic exchange between cells.
Authors Valadi H, Ekstrom K, Bossios A, Sjostrand M, Lee JJ, Lotvall JO
Journal name NCB
Publication year 2007
Sample Mast cells
Sample name MC9
Bone marrow-derived mast cells
HMC-1
Isolation/purification methods Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.11-1.21 g/mL
Molecules identified in the study Protein
mRNA
miRNA
Methods used in the study Mass spectrometry [MALDI TOF]
Western blotting
Microarray
miRCURY LNA Array
2
Experiment ID 15
ISEV standards
EM
EV Biophysical techniques
EV Cytosolic markers
CD63
EV Membrane markers
EV Negative markers
EV Particle analysis
Identified molecule mrna
Identification method Microarray
PubMed ID 17486113    
Organism Mus musculus
Homo sapiens
Experiment description Exosome-mediated transfer of mRNAs and microRNAs is a novel mechanism of genetic exchange between cells.
Authors Valadi H, Ekstrom K, Bossios A, Sjostrand M, Lee JJ, Lotvall JO
Journal name NCB
Publication year 2007
Sample Mast cells
Sample name MC9
Bone marrow-derived mast cells
HMC-1
Isolation/purification methods Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.11-1.21 g/mL
Molecules identified in the study Protein
mRNA
miRNA
Methods used in the study Mass spectrometry [MALDI TOF]
Western blotting
Microarray
miRCURY LNA Array
3
Experiment ID 188
ISEV standards
EM
EV Biophysical techniques
GAPDH|UCHL1|HSP90
EV Cytosolic markers
EV Membrane markers
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 19351151    
Organism Mus musculus
Experiment description Characterization of vesicles secreted from insulinoma NIT-1 cells.
Authors Lee HS, Jeong J, Lee KJ.
Journal name J Proteome Res
Publication year 2009
Sample Pancreatic cells
Sample name Pancreatic beta cell (NIT-1)
Isolation/purification methods Differential centrifugation
Sucrose density gradient
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for Rps6
  Protein Interactor ExoCarta ID Identification method PubMed Species
No interactions are found.



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