Gene description for P4hb
Gene name prolyl 4-hydroxylase, beta polypeptide
Gene symbol P4hb
Other names/aliases ERp59
PDI
Pdia1
Thbp
Species Mus musculus
 Database cross references - P4hb
ExoCarta ExoCarta_18453
Entrez Gene 18453
UniProt P09103  
 P4hb identified in exosomes derived from the following tissue/cell type
Embryonic fibroblasts 18494037    
Mast cells 17486113    
Pancreatic cells 19351151    
 Gene ontology annotations for P4hb
Molecular Function
    poly(A) RNA binding GO:0044822 ISO
    isomerase activity GO:0016853 IEA
    protein disulfide isomerase activity GO:0003756 ISO
    procollagen-proline 4-dioxygenase activity GO:0004656 ISO
    peptidyl-proline 4-dioxygenase activity GO:0031545 IBA
    protein heterodimerization activity GO:0046982 ISO
    enzyme binding GO:0019899 ISO
Biological Process
    cell redox homeostasis GO:0045454 IEA
    regulation of oxidative stress-induced intrinsic apoptotic signaling pathway GO:1902175 ISO
    peptidyl-proline hydroxylation to 4-hydroxy-L-proline GO:0018401 ISO
    response to endoplasmic reticulum stress GO:0034976 ISO
    cellular response to hypoxia GO:0071456 ISO
    protein folding GO:0006457 ISO
Subcellular Localization
    extracellular exosome GO:0070062 ISO
    plasma membrane GO:0005886 IEA
    membrane GO:0016020 IEA
    procollagen-proline 4-dioxygenase complex GO:0016222 ISO
    focal adhesion GO:0005925 ISO
    endoplasmic reticulum GO:0005783 ISO
    endoplasmic reticulum chaperone complex GO:0034663 IDA
    endoplasmic reticulum-Golgi intermediate compartment GO:0005793 ISO
 Experiment description of studies that identified P4hb in exosomes
1
Experiment ID 73
ISEV standards
EM
EV Biophysical techniques
HSP70|HSP90
EV Cytosolic markers
EV Membrane markers
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 18494037    
Organism Mus musculus
Experiment description Difference gel electrophoresis analysis of Ras-transformed fibroblast cell-derived exosomes.
Authors Ji H, Erfani N, Tauro BJ, Kapp EA, Zhu HJ, Moritz RL, Lim JW, Simpson RJ
Journal name ELEC
Publication year 2008
Sample Embryonic fibroblasts
Sample name NIH3T3
Isolation/purification methods Differential centrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [LCQ DECA]
Western blotting
2
Experiment ID 15
ISEV standards
EM
EV Biophysical techniques
EV Cytosolic markers
CD63
EV Membrane markers
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 17486113    
Organism Mus musculus
Homo sapiens
Experiment description Exosome-mediated transfer of mRNAs and microRNAs is a novel mechanism of genetic exchange between cells.
Authors Valadi H, Ekstrom K, Bossios A, Sjostrand M, Lee JJ, Lotvall JO
Journal name NCB
Publication year 2007
Sample Mast cells
Sample name MC9
Bone marrow-derived mast cells
HMC-1
Isolation/purification methods Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.11-1.21 g/mL
Molecules identified in the study Protein
mRNA
miRNA
Methods used in the study Mass spectrometry [MALDI TOF]
Western blotting
Microarray
miRCURY LNA Array
3
Experiment ID 188
ISEV standards
EM
EV Biophysical techniques
GAPDH|UCHL1|HSP90
EV Cytosolic markers
EV Membrane markers
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 19351151    
Organism Mus musculus
Experiment description Characterization of vesicles secreted from insulinoma NIT-1 cells.
Authors Lee HS, Jeong J, Lee KJ.
Journal name J Proteome Res
Publication year 2009
Sample Pancreatic cells
Sample name Pancreatic beta cell (NIT-1)
Isolation/purification methods Differential centrifugation
Sucrose density gradient
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for P4hb
  Protein Interactor ExoCarta ID Identification method PubMed Species
No interactions are found.



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