Gene description for Top1
Gene name topoisomerase (DNA) I
Gene symbol Top1
Other names/aliases AI467334
D130064I21Rik
Top-1
Species Mus musculus
 Database cross references - Top1
ExoCarta ExoCarta_21969
Entrez Gene 21969
UniProt Q04750  
 Top1 identified in exosomes derived from the following tissue/cell type
Neural stem cells 25242146    
 Gene ontology annotations for Top1
Molecular Function
    protein complex binding GO:0032403 ISO
    DNA topoisomerase type II (ATP-hydrolyzing) activity GO:0003918 IEA
    poly(A) RNA binding GO:0044822 ISO
    DNA topoisomerase type I activity GO:0003917 ISO
    chromatin DNA binding GO:0031490 ISO
    DNA binding GO:0003677 ISO
    DNA topoisomerase activity GO:0003916 IEA
    chromatin binding GO:0003682 ISO
    core promoter sequence-specific DNA binding GO:0001046 ISO
    isomerase activity GO:0016853 IEA
Biological Process
    circadian regulation of gene expression GO:0032922 ISO
    chromosome segregation GO:0007059 IBA
    DNA topological change GO:0006265 ISO
    chromatin remodeling GO:0006338 ISO
    embryonic cleavage GO:0040016 IMP
    DNA replication GO:0006260 IMP
    rRNA transcription GO:0009303 ISO
    rhythmic process GO:0048511 IEA
Subcellular Localization
    nuclear chromosome GO:0000228 ISO
    nucleoplasm GO:0005654 ISO
    perikaryon GO:0043204 IDA
    replication fork protection complex GO:0031298 IBA
    fibrillar center GO:0001650 ISO
    chromosome GO:0005694 IEA
    cytoplasmic mRNA processing body GO:0000932 ISO
    cytoplasm GO:0005737 IDA
    nucleus GO:0005634 ISO
    dense fibrillar component GO:0001651 ISO
    nucleolus GO:0005730 ISO
 Experiment description of studies that identified Top1 in exosomes
1
Experiment ID 263
ISEV standards
EV Biophysical techniques
EV Cytosolic markers
CD63|CD9
EV Membrane markers
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25242146    
Organism Mus musculus
Experiment description Extracellular Vesicles from Neural Stem Cells Transfer IFN-γ via Ifngr1 to Activate Stat1 Signaling in Target Cells
Authors Chiara Cossetti, Nunzio Iraci, Tim R. Mercer, Tommaso Leonardi, Emanuele Alpi, Denise Drago, Clara Alfaro-Cervello, Harpreet K. Saini, Matthew P. Davis, Julia Schaeffer, Beatriz Vega, Matilde Stefanini, CongJian Zhao, Werner Muller, Jose Manuel Garcia-Verdugo, Suresh Mathivanan, Angela Bachi, Anton J. Enright, John S. Mattick, Stefano Pluchino
Journal name Molecular Cell
Publication year 2014
Sample Neural stem cells
Sample name NPCs - Th1 treated
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.20 g/mL
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for Top1
  Protein Interactor ExoCarta ID Identification method PubMed Species
No interactions are found.
 Pathways in which Top1 is involved
No pathways found





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