Gene description for Rpl22
Gene name ribosomal protein L22
Gene symbol Rpl22
Other names/aliases 2700038K18Rik
Species Mus musculus
 Database cross references - Rpl22
ExoCarta ExoCarta_19934
Vesiclepedia VP_19934
Entrez Gene 19934
UniProt P67984  
 Rpl22 identified in sEVs derived from the following tissue/cell type
Colon cancer cells 37309723    
Mast cells 17486113    
Melanoma cells 34957415    
Melanoma cells 34957415    
T-cell lymphoma cells 37309723    
 Gene ontology annotations for Rpl22
Molecular Function
    RNA binding GO:0003723 IBA
    RNA binding GO:0003723 IEA
    structural constituent of ribosome GO:0003735 IBA
    structural constituent of ribosome GO:0003735 IDA
    structural constituent of ribosome GO:0003735 ISO
    heparin binding GO:0008201 IDA
    identical protein binding GO:0042802 IEA
    identical protein binding GO:0042802 ISO
Biological Process
    cytoplasmic translation GO:0002181 IBA
    alpha-beta T cell differentiation GO:0046632 IMP
    translation at presynapse GO:0140236 IDA
    translation at presynapse GO:0140236 IMP
    translation at postsynapse GO:0140242 NAS
Subcellular Localization
    nucleus GO:0005634 IEA
    nucleus GO:0005634 ISO
    cytoplasm GO:0005737 ISS
    cytosol GO:0005829 TAS
    ribosome GO:0005840 IDA
    ribosome GO:0005840 IMP
    ribosome GO:0005840 ISO
    ribosome GO:0005840 ISS
    ribosome GO:0005840 NAS
    cytosolic large ribosomal subunit GO:0022625 IDA
    cytosolic large ribosomal subunit GO:0022625 ISO
    cytosolic ribosome GO:0022626 ISO
    synapse GO:0045202 IDA
    presynapse GO:0098793 IDA
    presynapse GO:0098793 IMP
    postsynapse GO:0098794 NAS
    glutamatergic synapse GO:0098978 IDA
    glutamatergic synapse GO:0098978 IMP
    ribonucleoprotein complex GO:1990904 ISO
 Experiment description of studies that identified Rpl22 in sEVs
1
Experiment ID 907
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample Colon cancer cells
Sample name C26
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
2
Experiment ID 15
MISEV standards
✔
EM
Biophysical techniques
✔
CD63
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 17486113    
Organism Mus musculus
Homo sapiens
Experiment description Exosome-mediated transfer of mRNAs and microRNAs is a novel mechanism of genetic exchange between cells.
Authors "Valadi H, Ekstrom K, Bossios A, Sjostrand M, Lee JJ, Lotvall JO"
Journal name NCB
Publication year 2007
Sample Mast cells
Sample name MC9
Bone marrow-derived mast cells
HMC-1
Isolation/purification methods Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.11-1.21 g/mL
Molecules identified in the study Protein
mRNA
miRNA
Methods used in the study Mass spectrometry [MALDI TOF]
Western blotting
Microarray
miRCURY LNA Array
3
Experiment ID 15
MISEV standards
✔
EM
Biophysical techniques
✔
CD63
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule mrna
Identification method Microarray
PubMed ID 17486113    
Organism Mus musculus
Homo sapiens
Experiment description Exosome-mediated transfer of mRNAs and microRNAs is a novel mechanism of genetic exchange between cells.
Authors "Valadi H, Ekstrom K, Bossios A, Sjostrand M, Lee JJ, Lotvall JO"
Journal name NCB
Publication year 2007
Sample Mast cells
Sample name MC9
Bone marrow-derived mast cells
HMC-1
Isolation/purification methods Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.11-1.21 g/mL
Molecules identified in the study Protein
mRNA
miRNA
Methods used in the study Mass spectrometry [MALDI TOF]
Western blotting
Microarray
miRCURY LNA Array
4
Experiment ID 1117
MISEV standards
✘ Biophysical techniques
✔
Cd81|Icam1
Enriched markers
✘ Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 34957415    
Organism Mus musculus
Experiment description Melanoma-derived small extracellular vesicles induce lymphangiogenesis and metastasis through an NGFR-dependent mechanism
Authors "García-Silva S, Benito-Martín A, Nogués L, Hernández-Barranco A, Mazariegos MS, Santos V, Hergueta-Redondo M, Ximénez-Embún P, Kataru RP, Lopez AA, Merino C, Sánchez-Redondo S, Graña-Castro O, Matei I, Nicolás-Avila JÁ, Torres-Ruiz R, Rodríguez-Perales S, Martínez L, Pérez-Martínez M, Mata G, Szumera-Ciećkiewicz A, Kalinowska I, Saltari A, Martínez-Gómez JM, Hogan SA, Saragovi HU, Ortega S, Garcia-Martin C, Boskovic J, Levesque MP, Rutkowski P, Hidalgo A, Muñoz J, Megías D, Mehrara BJ, Lyden D, Peinado H."
Journal name Nat Cancer
Publication year 2021
Sample Melanoma cells
Sample name B16-F1R2
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
5
Experiment ID 1118
MISEV standards
✘ Biophysical techniques
✔
Cd81|Icam1
Enriched markers
✘ Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 34957415    
Organism Mus musculus
Experiment description Melanoma-derived small extracellular vesicles induce lymphangiogenesis and metastasis through an NGFR-dependent mechanism
Authors "García-Silva S, Benito-Martín A, Nogués L, Hernández-Barranco A, Mazariegos MS, Santos V, Hergueta-Redondo M, Ximénez-Embún P, Kataru RP, Lopez AA, Merino C, Sánchez-Redondo S, Graña-Castro O, Matei I, Nicolás-Avila JÁ, Torres-Ruiz R, Rodríguez-Perales S, Martínez L, Pérez-Martínez M, Mata G, Szumera-Ciećkiewicz A, Kalinowska I, Saltari A, Martínez-Gómez JM, Hogan SA, Saragovi HU, Ortega S, Garcia-Martin C, Boskovic J, Levesque MP, Rutkowski P, Hidalgo A, Muñoz J, Megías D, Mehrara BJ, Lyden D, Peinado H."
Journal name Nat Cancer
Publication year 2021
Sample Melanoma cells
Sample name B16-F1R2
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
6
Experiment ID 908
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample T-cell lymphoma cells
Sample name EL4
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for Rpl22
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 Rps3 27050
Co-fractionation Mus musculus
2 Rpl28 19943
Co-fractionation Mus musculus
3 Lars 107045
Co-fractionation Mus musculus
4 Rpl27 19942
Co-fractionation Mus musculus
5 Rpl6 19988
Co-fractionation Mus musculus
6 Rps24 20088
Co-fractionation Mus musculus
7 Actb 11461
Co-fractionation Mus musculus
8 Rcsd1  
Co-fractionation Mus musculus
9 Rpl35a 57808
Co-fractionation Mus musculus
10 Tmem173  
Proximity Label-MS Mus musculus
11 Rpl7 19989
Co-fractionation Mus musculus
12 Plod3 26433
Co-fractionation Mus musculus
13 Casp6 12368
Co-fractionation Mus musculus
14 Rpl36a 19982
Co-fractionation Mus musculus
15 Rpl26 19941
Co-fractionation Mus musculus
16 Rpsa 16785
Co-fractionation Mus musculus
17 Rpl3 27367
Co-fractionation Mus musculus
18 Abce1 24015
Co-fractionation Mus musculus
19 Rpl11 67025
Co-fractionation Mus musculus
20 Eed  
Affinity Capture-MS Mus musculus
21 Rpl31 114641
Co-fractionation Mus musculus
22 Rplp2 67186
Co-fractionation Mus musculus
23 Rps4x 20102
Co-fractionation Mus musculus
24 Rps23 66475
Co-fractionation Mus musculus
25 Scyl2  
Co-fractionation Mus musculus
26 Tmem165  
Co-fractionation Mus musculus
27 Tfe3  
Affinity Capture-MS Mus musculus
28 Rpl24 68193
Co-fractionation Mus musculus
29 Rps7 20115
Co-fractionation Mus musculus
30 Rpl17 319195
Co-fractionation Mus musculus
31 Rpl5 100503670
Co-fractionation Mus musculus
32 Gtf2f1  
Co-fractionation Mus musculus
33 Rps5 20103
Co-fractionation Mus musculus
34 Rps15a 267019
Co-fractionation Mus musculus
35 Fancd2  
Affinity Capture-MS Mus musculus
36 Rplp0 11837
Co-fractionation Mus musculus
37 Rps6 20104
Co-fractionation Mus musculus
38 Rpl30 19946
Co-fractionation Mus musculus
39 Rpl39 67248
Co-fractionation Mus musculus
40 Ube2i  
Two-hybrid Mus musculus
41 Rps16 20055
Co-fractionation Mus musculus
42 Zfp330  
Co-fractionation Mus musculus
43 Rps17 20068
Co-fractionation Mus musculus
44 Sf3a3 75062
Co-fractionation Mus musculus
45 Dhx29  
Co-fractionation Mus musculus
46 Rps18 20084
Co-fractionation Mus musculus
47 Rpl13a 22121
Co-fractionation Mus musculus
48 Rpl14 67115
Co-fractionation Mus musculus
49 Rps2 16898
Co-fractionation Mus musculus
50 Rps8 20116
Co-fractionation Mus musculus
51 Rpl7a 27176
Co-fractionation Mus musculus
52 Pde12  
Co-fractionation Mus musculus
53 Rpl32 19951
Co-fractionation Mus musculus
54 Rps28 54127
Co-fractionation Mus musculus
55 Rps11 27207
Co-fractionation Mus musculus
56 Rpl18a 76808
Co-fractionation Mus musculus
57 Rpl23 65019
Co-fractionation Mus musculus
58 Lrrc59  
Co-fractionation Mus musculus
59 Cbx5  
Affinity Capture-MS Mus musculus
60 Rpl38  
Co-fractionation Mus musculus
61 Rps3a1 20091
Co-fractionation Mus musculus
62 Rps21 66481
Co-fractionation Mus musculus
63 Rps14 20044
Co-fractionation Mus musculus
64 Rps9 76846
Co-fractionation Mus musculus
65 Rpl35 66489
Co-fractionation Mus musculus
66 Dhfr 13361
Co-fractionation Mus musculus
67 Rpl29 19944
Co-fractionation Mus musculus
68 Rps20 67427
Co-fractionation Mus musculus
69 Rps27a 78294
Co-fractionation Mus musculus
70 Rps25 75617
Co-fractionation Mus musculus
71 Rpl10a 19896
Co-fractionation Mus musculus
72 Top1 21969
Co-fractionation Mus musculus
73 Kctd13  
Affinity Capture-MS Mus musculus
74 Prpf3 70767
Co-fractionation Mus musculus
75 Fzr1  
Affinity Capture-MS Mus musculus
76 Rpl12 269261
Co-fractionation Mus musculus
77 Rpl18 19899
Co-fractionation Mus musculus
78 Gnb2l1 14694
Co-fractionation Mus musculus
79 Rps15 20054
Co-fractionation Mus musculus
80 Npm1 18148
Affinity Capture-MS Mus musculus
81 Rps13 68052
Co-fractionation Mus musculus
82 Tcf25  
Co-fractionation Mus musculus
83 Rps26 27370
Co-fractionation Mus musculus
84 Rpl23a 268449
Co-fractionation Mus musculus
85 Rpl15 66480
Co-fractionation Mus musculus
86 Eef1b2 55949
Co-fractionation Mus musculus
87 Rbp2  
Co-fractionation Mus musculus
88 Rpl4 67891
Co-fractionation Mus musculus
89 Mlec  
Co-fractionation Mus musculus
90 Rpl13 270106
Co-fractionation Mus musculus
91 Rpl8 26961
Co-fractionation Mus musculus
View the network image/svg+xml



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