Gene description for Eef1b2
Gene name eukaryotic translation elongation factor 1 beta 2
Gene symbol Eef1b2
Other names/aliases 2810017J07Rik
Eef1b
Species Mus musculus
 Database cross references - Eef1b2
ExoCarta ExoCarta_55949
Entrez Gene 55949
UniProt O70251  
 Eef1b2 identified in exosomes derived from the following tissue/cell type
Brain cancer cells 19109410    
Fibroblasts 23260141    
Pancreatic cells 19351151    
 Gene ontology annotations for Eef1b2
Molecular Function
    protein binding GO:0005515 IPI
    translation elongation factor activity GO:0003746 ISS
Biological Process
    translational elongation GO:0006414 ISS
    translation GO:0006412 ISS
Subcellular Localization
    eukaryotic translation elongation factor 1 complex GO:0005853 ISS
    cytoplasm GO:0005737 ISO
    endoplasmic reticulum GO:0005783 ISO
 Experiment description of studies that identified Eef1b2 in exosomes
1
Experiment ID 26
ISEV standards
EM
EV Biophysical techniques
Alix|GAPDH|HSP70
EV Cytosolic markers
CD9
EV Membrane markers
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 19109410    
Organism Mus musculus
Experiment description Proteomic and immunologic analyses of brain tumor exosomes.
Authors Graner MW, Alzate O, Dechkovskaia AM, Keene JD, Sampson JH, Mitchell DA, Bigner DD
Journal name FASEB
Publication year 2008
Sample Brain cancer cells
Sample name SMA560vIII
Isolation/purification methods Differential centrifugation
Sucrose density gradient
Flotation density 1.13-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [MALDI TOF/TOF]
Western blotting
2
Experiment ID 210
ISEV standards
EM
EV Biophysical techniques
EV Cytosolic markers
CD81|FLOT1
EV Membrane markers
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23260141    
Organism Mus musculus
Experiment description Exosomes Mediate Stromal Mobilization of Autocrine Wnt-PCP Signaling in Breast Cancer Cell Migration.
Authors Luga V, Zhang L, Viloria-Petit AM, Ogunjimi AA, Inanlou MR, Chiu E, Buchanan M, Hosein AN, Basik M, Wrana JL.
Journal name Cell
Publication year 2012
Sample Fibroblasts
Sample name Normal-Fibroblasts (L cells)
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
3
Experiment ID 188
ISEV standards
EM
EV Biophysical techniques
GAPDH|UCHL1|HSP90
EV Cytosolic markers
EV Membrane markers
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 19351151    
Organism Mus musculus
Experiment description Characterization of vesicles secreted from insulinoma NIT-1 cells.
Authors Lee HS, Jeong J, Lee KJ.
Journal name J Proteome Res
Publication year 2009
Sample Pancreatic cells
Sample name Pancreatic beta cell (NIT-1)
Isolation/purification methods Differential centrifugation
Sucrose density gradient
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for Eef1b2
  Protein Interactor ExoCarta ID Identification method PubMed Species
No interactions are found.
 Pathways in which Eef1b2 is involved
PathwayEvidenceSource
Eukaryotic Translation Elongation IEA Reactome





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