Gene description for Rps3
Gene name ribosomal protein S3
Gene symbol Rps3
Other names/aliases D7Ertd795e
Rs_3
Species Mus musculus
 Database cross references - Rps3
ExoCarta ExoCarta_27050
Entrez Gene 27050
UniProt P62908  
 Rps3 identified in exosomes derived from the following tissue/cell type
Fibroblasts 23260141    
Mast cells 17486113    
Mov neuroglial cells 15210972    
Pancreatic cells 19351151    
 Gene ontology annotations for Rps3
Molecular Function
    protein kinase binding GO:0019901 ISO
    poly(A) RNA binding GO:0044822 ISO
    protein binding GO:0005515 IPI
    RNA binding GO:0003723 IEA
    enzyme binding GO:0019899 ISO
    oxidized purine nucleobase lesion DNA N-glycosylase activity GO:0008534 IBA
    protein kinase A binding GO:0051018 ISO
    nucleic acid binding GO:0003676 IEA
    damaged DNA binding GO:0003684 ISO
    NF-kappaB binding GO:0051059 ISO
    structural constituent of ribosome GO:0003735 ISO
    DNA binding GO:0003677 IEA
    lyase activity GO:0016829 IEA
    DNA-(apurinic or apyrimidinic site) lyase activity GO:0003906 ISO
    mRNA binding GO:0003729 ISO
Biological Process
    cell cycle GO:0007049 IEA
    DNA repair GO:0006281 IBA
    positive regulation of NF-kappaB transcription factor activity GO:0051092 ISO
    apoptotic process GO:0006915 IEA
    translation GO:0006412 IEA
    transcription, DNA-templated GO:0006351 IEA
    cell division GO:0051301 IEA
    positive regulation of DNA N-glycosylase activity GO:1902546 ISO
    positive regulation of apoptotic signaling pathway GO:2001235 ISO
    cellular response to DNA damage stimulus GO:0006974 IEA
    regulation of translation GO:0006417 IEA
    regulation of transcription, DNA-templated GO:0006355 IEA
    negative regulation of DNA repair GO:0045738 ISO
    DNA metabolic process GO:0006259 ISO
    nucleic acid phosphodiester bond hydrolysis GO:0090305 IBA
    mitotic nuclear division GO:0007067 IEA
Subcellular Localization
    dendrite GO:0030425 ISO
    cytoplasm GO:0005737 ISO
    ribonucleoprotein complex GO:0030529 ISO
    cytosolic small ribosomal subunit GO:0022627 ISO
    cytoskeleton GO:0005856 IEA
    nucleus GO:0005634 ISO
    extracellular exosome GO:0070062 ISO
    cytosol GO:0005829 ISO
    membrane GO:0016020 ISO
    ribosome GO:0005840 IEA
    small ribosomal subunit GO:0015935 IEA
    NF-kappaB complex GO:0071159 ISO
    mitochondrion GO:0005739 IEA
    ruffle membrane GO:0032587 ISO
    mitochondrial inner membrane GO:0005743 IEA
    endomembrane system GO:0012505 ISO
    focal adhesion GO:0005925 ISO
 Experiment description of studies that identified Rps3 in exosomes
1
Experiment ID 210
ISEV standards
✔
EM
EV Biophysical techniques
✘ EV Cytosolic markers
✔
CD81|FLOT1
EV Membrane markers
✘ EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23260141    
Organism Mus musculus
Experiment description Exosomes Mediate Stromal Mobilization of Autocrine Wnt-PCP Signaling in Breast Cancer Cell Migration.
Authors Luga V, Zhang L, Viloria-Petit AM, Ogunjimi AA, Inanlou MR, Chiu E, Buchanan M, Hosein AN, Basik M, Wrana JL.
Journal name Cell
Publication year 2012
Sample Fibroblasts
Sample name Normal-Fibroblasts (L cells)
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
2
Experiment ID 15
ISEV standards
✔
EM
EV Biophysical techniques
✘ EV Cytosolic markers
✔
CD63
EV Membrane markers
✘ EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 17486113    
Organism Mus musculus
Homo sapiens
Experiment description Exosome-mediated transfer of mRNAs and microRNAs is a novel mechanism of genetic exchange between cells.
Authors Valadi H, Ekstrom K, Bossios A, Sjostrand M, Lee JJ, Lotvall JO
Journal name NCB
Publication year 2007
Sample Mast cells
Sample name MC9
Bone marrow-derived mast cells
HMC-1
Isolation/purification methods Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.11-1.21 g/mL
Molecules identified in the study Protein
mRNA
miRNA
Methods used in the study Mass spectrometry [MALDI TOF]
Western blotting
Microarray
miRCURY LNA Array
3
Experiment ID 39
ISEV standards
✔
IEM
EV Biophysical techniques
✔
TSG101|HSC70
EV Cytosolic markers
✔
FLOT1
EV Membrane markers
✘ EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 15210972    
Organism Mus musculus
Experiment description Cells release prions in association with exosomes.
Authors Fevrier B, Vilette D, Archer F, Loew D, Faigle W, Vidal M, Laude H, Raposo G
Journal name PNAS
Publication year 2004
Sample Mov neuroglial cells
Sample name Mov neuroglial cell
Isolation/purification methods Differential centrifugation
Sucrose density gradient
Flotation density 1.14 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [QSTAR]
Western blotting
Immunoelectron Microscopy
4
Experiment ID 188
ISEV standards
✔
EM
EV Biophysical techniques
✔
GAPDH|UCHL1|HSP90
EV Cytosolic markers
✘ EV Membrane markers
✘ EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 19351151    
Organism Mus musculus
Experiment description Characterization of vesicles secreted from insulinoma NIT-1 cells.
Authors Lee HS, Jeong J, Lee KJ.
Journal name J Proteome Res
Publication year 2009
Sample Pancreatic cells
Sample name Pancreatic beta cell (NIT-1)
Isolation/purification methods Differential centrifugation
Sucrose density gradient
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for Rps3
  Protein Interactor ExoCarta ID Identification method PubMed Species
No interactions are found.



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