Gene description for Calr
Gene name calreticulin
Gene symbol Calr
Other names/aliases CRT
Calregulin
Species Mus musculus
 Database cross references - Calr
ExoCarta ExoCarta_12317
Entrez Gene 12317
UniProt P14211  
 Calr identified in exosomes derived from the following tissue/cell type
Brain cancer cells 19109410    
Pancreatic cells 19351151    
 Gene ontology annotations for Calr
Molecular Function
    unfolded protein binding GO:0051082 ISO
    glycoprotein binding GO:0001948 ISO
    hormone binding GO:0042562 ISO
    androgen receptor binding GO:0050681 ISO
    protein binding GO:0005515 IPI
    carbohydrate binding GO:0030246 IDA
    iron ion binding GO:0005506 ISO
    metal ion binding GO:0046872 IEA
    calcium ion binding GO:0005509 ISO
    poly(A) RNA binding GO:0044822 ISO
    ubiquitin protein ligase binding GO:0031625 ISO
    mRNA binding GO:0003729 ISO
    integrin binding GO:0005178 ISO
    peptide binding GO:0042277 ISO
Biological Process
    positive regulation of substrate adhesion-dependent cell spreading GO:1900026 ISO
    chaperone-mediated protein folding GO:0061077 ISO
    peptide antigen assembly with MHC class I protein complex GO:0002502 IMP
    negative regulation of retinoic acid receptor signaling pathway GO:0048387 ISO
    positive regulation of DNA replication GO:0045740 ISO
    positive regulation of cell proliferation GO:0008284 ISO
    cell cycle arrest GO:0007050 ISO
    regulation of meiotic nuclear division GO:0040020 IDA
    positive regulation of phagocytosis GO:0050766 IMP
    positive regulation of dendritic cell chemotaxis GO:2000510 ISO
    protein stabilization GO:0050821 IDA
    negative regulation of transcription, DNA-templated GO:0045892 ISO
    negative regulation of translation GO:0017148 ISO
    protein export from nucleus GO:0006611 ISO
    negative regulation of intracellular steroid hormone receptor signaling pathway GO:0033144 ISO
    protein folding GO:0006457 IEA
    positive regulation of cell cycle GO:0045787 ISO
    protein localization to nucleus GO:0034504 ISO
    positive regulation of gene expression GO:0010628 IMP
    cellular senescence GO:0090398 ISO
    negative regulation of transcription from RNA polymerase II promoter GO:0000122 ISO
    cortical actin cytoskeleton organization GO:0030866 IDA
    negative regulation of neuron differentiation GO:0045665 ISO
Subcellular Localization
    cytoplasm GO:0005737 ISO
    nucleus GO:0005634 ISO
    endoplasmic reticulum GO:0005783 ISS
    smooth endoplasmic reticulum GO:0005790 ISO
    intracellular membrane-bounded organelle GO:0043231 ISO
    focal adhesion GO:0005925 ISO
    extracellular exosome GO:0070062 ISO
    Golgi apparatus GO:0005794 ISO
    perinuclear region of cytoplasm GO:0048471 ISO
    cytosol GO:0005829 ISO
    acrosomal vesicle GO:0001669 ISO
    sarcoplasmic reticulum GO:0016529 ISO
    membrane GO:0016020 ISO
    MHC class I peptide loading complex GO:0042824 IMP
    cell surface GO:0009986 ISO
    endoplasmic reticulum lumen GO:0005788 ISO
    protein complex GO:0043234 ISO
    extracellular space GO:0005615 ISO
    extracellular matrix GO:0031012 ISO
    polysome GO:0005844 IDA
    external side of plasma membrane GO:0009897 IDA
 Experiment description of studies that identified Calr in exosomes
1
Experiment ID 26
ISEV standards
EM
EV Biophysical techniques
Alix|GAPDH|HSP70
EV Cytosolic markers
CD9
EV Membrane markers
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Western blotting
PubMed ID 19109410    
Organism Mus musculus
Experiment description Proteomic and immunologic analyses of brain tumor exosomes.
Authors Graner MW, Alzate O, Dechkovskaia AM, Keene JD, Sampson JH, Mitchell DA, Bigner DD
Journal name FASEB
Publication year 2008
Sample Brain cancer cells
Sample name SMA560vIII
Isolation/purification methods Differential centrifugation
Sucrose density gradient
Flotation density 1.13-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [MALDI TOF/TOF]
Western blotting
2
Experiment ID 188
ISEV standards
EM
EV Biophysical techniques
GAPDH|UCHL1|HSP90
EV Cytosolic markers
EV Membrane markers
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 19351151    
Organism Mus musculus
Experiment description Characterization of vesicles secreted from insulinoma NIT-1 cells.
Authors Lee HS, Jeong J, Lee KJ.
Journal name J Proteome Res
Publication year 2009
Sample Pancreatic cells
Sample name Pancreatic beta cell (NIT-1)
Isolation/purification methods Differential centrifugation
Sucrose density gradient
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for Calr
  Protein Interactor ExoCarta ID Identification method PubMed Species
No interactions are found.



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