Gene description for App
Gene name amyloid beta (A4) precursor protein
Gene symbol App
Other names/aliases Abeta
Abpp
Adap
Ag
Cvap
E030013M08Rik
betaApp
Species Mus musculus
 Database cross references - App
ExoCarta ExoCarta_11820
Vesiclepedia VP_11820
Entrez Gene 11820
UniProt P12023  
 App identified in exosomes derived from the following tissue/cell type
Colon cancer cells 37309723    
Fibroblasts 23260141    
Mast cells 17486113    
T-cell lymphoma cells 37309723    
 Gene ontology annotations for App
Molecular Function
    serine-type endopeptidase inhibitor activity GO:0004867 ISO
    peptidase inhibitor activity GO:0030414 IEA
    DNA binding GO:0003677 IDA
    enzyme binding GO:0019899 ISO
    peptidase activator activity GO:0016504 ISO
    protein binding GO:0005515 IPI
    receptor binding GO:0005102 ISO
    growth factor receptor binding GO:0070851 ISO
    identical protein binding GO:0042802 ISO
    metal ion binding GO:0046872 IEA
    PTB domain binding GO:0051425 ISO
    acetylcholine receptor binding GO:0033130 ISO
    heparin binding GO:0008201 IEA
    transition metal ion binding GO:0046914 IEA
Biological Process
    axonogenesis GO:0007409 IMP
    endocytosis GO:0006897 IMP
    locomotory behavior GO:0007626 IGI
    neuron remodeling GO:0016322 IMP
    positive regulation of mitotic cell cycle GO:0045931 IMP
    Notch signaling pathway GO:0007219 IEA
    synaptic growth at neuromuscular junction GO:0051124 IGI
    regulation of translation GO:0006417 IDA
    forebrain development GO:0030900 IMP
    negative regulation of neuron differentiation GO:0045665 IGI
    negative regulation of endopeptidase activity GO:0010951 ISO
    regulation of synapse structure or activity GO:0050803 IMP
    visual learning GO:0008542 IMP
    protein homooligomerization GO:0051260 ISO
    neuron apoptotic process GO:0051402 ISO
    negative regulation of peptidase activity GO:0010466 IEA
    mRNA polyadenylation GO:0006378 IDA
    collateral sprouting in absence of injury GO:0048669 IGI
    extracellular matrix organization GO:0030198 IGI
    dendrite development GO:0016358 IMP
    regulation of multicellular organism growth GO:0040014 IMP
    positive regulation of transcription from RNA polymerase II promoter GO:0045944 IGI
    mating behavior GO:0007617 IGI
    adult locomotory behavior GO:0008344 IMP
    protein phosphorylation GO:0006468 IMP
    apoptotic process GO:0006915 IEA
    axon cargo transport GO:0008088 IMP
    cholesterol metabolic process GO:0008203 IMP
    neuron projection development GO:0031175 IDA
    nervous system development GO:0007399 IEA
    regulation of gene expression GO:0010468 IDA
    axon midline choice point recognition GO:0016199 IMP
    cellular copper ion homeostasis GO:0006878 IMP
    regulation of protein binding GO:0043393 IMP
    positive regulation of peptidase activity GO:0010952 ISO
    neuromuscular process controlling balance GO:0050885 IGI
    smooth endoplasmic reticulum calcium ion homeostasis GO:0051563 IGI
    ionotropic glutamate receptor signaling pathway GO:0035235 IMP
    positive regulation of G2/M transition of mitotic cell cycle GO:0010971 IMP
    response to oxidative stress GO:0006979 IGI
    suckling behavior GO:0001967 IGI
    regulation of epidermal growth factor-activated receptor activity GO:0007176 IGI
    cell adhesion GO:0007155 IEA
Subcellular Localization
    cytoplasm GO:0005737 ISO
    extracellular space GO:0005615 ISO
    ER to Golgi transport vesicle GO:0030134 IDA
    intracellular membrane-bounded organelle GO:0043231 ISO
    neuromuscular junction GO:0031594 IDA
    spindle midzone GO:0051233 IDA
    membrane raft GO:0045121 ISO
    ciliary rootlet GO:0035253 IDA
    apical part of cell GO:0045177 IDA
    cell GO:0005623 IGI
    endosome GO:0005768 ISO
    nuclear envelope lumen GO:0005641 ISO
    intracellular GO:0005622 IGI
    plasma membrane GO:0005886 ISO
    neuron projection GO:0043005 ISO
    cell surface GO:0009986 ISO
    axon GO:0030424 ISO
    terminal bouton GO:0043195 ISO
    membrane GO:0016020 TAS
    integral component of membrane GO:0016021 IDA
    smooth endoplasmic reticulum GO:0005790 IGI
    cell-cell junction GO:0005911 IDA
    Golgi apparatus GO:0005794 ISO
    perinuclear region of cytoplasm GO:0048471 ISO
    receptor complex GO:0043235 ISO
    cytoplasmic vesicle GO:0031410 IDA
    coated pit GO:0005905 IEA
    extracellular exosome GO:0070062 ISO
 Experiment description of studies that identified App in exosomes
1
Experiment ID 907
MISEV standards
✔
EM
EV Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
EV Enriched markers
✔
HSPA5
EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S.
Journal name Proteomics
Publication year 2023
Sample Colon cancer cells
Sample name C26
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
2
Experiment ID 210
MISEV standards
✔
EM
EV Biophysical techniques
✔
CD81|FLOT1
EV Enriched markers
✘ EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23260141    
Organism Mus musculus
Experiment description Exosomes Mediate Stromal Mobilization of Autocrine Wnt-PCP Signaling in Breast Cancer Cell Migration.
Authors Luga V, Zhang L, Viloria-Petit AM, Ogunjimi AA, Inanlou MR, Chiu E, Buchanan M, Hosein AN, Basik M, Wrana JL.
Journal name Cell
Publication year 2012
Sample Fibroblasts
Sample name Normal-Fibroblasts (L cells)
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
3
Experiment ID 15
MISEV standards
✔
EM
EV Biophysical techniques
✔
CD63
EV Enriched markers
✘ EV Negative markers
✘ EV Particle analysis
Identified molecule mrna
Identification method Microarray
PubMed ID 17486113    
Organism Mus musculus
Homo sapiens
Experiment description Exosome-mediated transfer of mRNAs and microRNAs is a novel mechanism of genetic exchange between cells.
Authors Valadi H, Ekstrom K, Bossios A, Sjostrand M, Lee JJ, Lotvall JO
Journal name NCB
Publication year 2007
Sample Mast cells
Sample name MC9
Bone marrow-derived mast cells
HMC-1
Isolation/purification methods Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.11-1.21 g/mL
Molecules identified in the study Protein
mRNA
miRNA
Methods used in the study Mass spectrometry [MALDI TOF]
Western blotting
Microarray
miRCURY LNA Array
4
Experiment ID 908
MISEV standards
✔
EM
EV Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
EV Enriched markers
✔
HSPA5
EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S.
Journal name Proteomics
Publication year 2023
Sample T-cell lymphoma cells
Sample name EL4
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for App
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 Kif1a  
Affinity Capture-Western Mus musculus
View the network image/svg+xml



Perform bioinformatics analysis of your extracellular vesicle data set using FunRich, a open access standalone tool. NEW UPDATED VERSION OF FunRich available for download (12/09/2016) from here