Gene description for Hsp90b1
Gene name heat shock protein 90, beta (Grp94), member 1
Gene symbol Hsp90b1
Other names/aliases ERp99
GRP94
TA-3
Targ2
Tra-1
Tra1
endoplasmin
gp96
Species Mus musculus
 Database cross references - Hsp90b1
ExoCarta ExoCarta_22027
Entrez Gene 22027
UniProt P08113  
 Hsp90b1 identified in exosomes derived from the following tissue/cell type
Embryonic fibroblasts 18494037    
Fibroblasts 23260141    
Mast cells 17486113    
 Gene ontology annotations for Hsp90b1
Molecular Function
    low-density lipoprotein particle receptor binding GO:0050750 ISO
    RNA binding GO:0003723 ISO
    protein binding GO:0005515 IPI
    virion binding GO:0046790 ISO
    protein phosphatase binding GO:0019903 ISO
    nucleotide binding GO:0000166 IEA
    unfolded protein binding GO:0051082 IEA
    ATP binding GO:0005524 IEA
Biological Process
    response to hypoxia GO:0001666 ISO
    actin rod assembly GO:0031247 ISO
    response to stress GO:0006950 IEA
    negative regulation of apoptotic process GO:0043066 ISO
    retrograde protein transport, ER to cytosol GO:0030970 ISO
    ER-associated ubiquitin-dependent protein catabolic process GO:0030433 ISO
    regulation of phosphoprotein phosphatase activity GO:0043666 ISO
    protein folding GO:0006457 IEA
    cellular response to ATP GO:0071318 ISO
Subcellular Localization
    endoplasmic reticulum membrane GO:0005789 ISO
    extracellular exosome GO:0070062 ISO
    smooth endoplasmic reticulum GO:0005790 ISO
    midbody GO:0030496 ISO
    plasma membrane GO:0005886 IDA
    nucleus GO:0005634 ISO
    perinuclear region of cytoplasm GO:0048471 ISO
    endoplasmic reticulum lumen GO:0005788 ISO
    extracellular matrix GO:0031012 ISO
    cytosol GO:0005829 ISO
    focal adhesion GO:0005925 ISO
    endoplasmic reticulum GO:0005783 ISO
    membrane GO:0016020 ISO
    endoplasmic reticulum chaperone complex GO:0034663 IDA
 Experiment description of studies that identified Hsp90b1 in exosomes
1
Experiment ID 73
ISEV standards
EM
EV Biophysical techniques
HSP70|HSP90
EV Cytosolic markers
EV Membrane markers
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 18494037    
Organism Mus musculus
Experiment description Difference gel electrophoresis analysis of Ras-transformed fibroblast cell-derived exosomes.
Authors Ji H, Erfani N, Tauro BJ, Kapp EA, Zhu HJ, Moritz RL, Lim JW, Simpson RJ
Journal name ELEC
Publication year 2008
Sample Embryonic fibroblasts
Sample name NIH3T3
Isolation/purification methods Differential centrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [LCQ DECA]
Western blotting
2
Experiment ID 210
ISEV standards
EM
EV Biophysical techniques
EV Cytosolic markers
CD81|FLOT1
EV Membrane markers
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23260141    
Organism Mus musculus
Experiment description Exosomes Mediate Stromal Mobilization of Autocrine Wnt-PCP Signaling in Breast Cancer Cell Migration.
Authors Luga V, Zhang L, Viloria-Petit AM, Ogunjimi AA, Inanlou MR, Chiu E, Buchanan M, Hosein AN, Basik M, Wrana JL.
Journal name Cell
Publication year 2012
Sample Fibroblasts
Sample name Normal-Fibroblasts (L cells)
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
3
Experiment ID 15
ISEV standards
EM
EV Biophysical techniques
EV Cytosolic markers
CD63
EV Membrane markers
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 17486113    
Organism Mus musculus
Homo sapiens
Experiment description Exosome-mediated transfer of mRNAs and microRNAs is a novel mechanism of genetic exchange between cells.
Authors Valadi H, Ekstrom K, Bossios A, Sjostrand M, Lee JJ, Lotvall JO
Journal name NCB
Publication year 2007
Sample Mast cells
Sample name MC9
Bone marrow-derived mast cells
HMC-1
Isolation/purification methods Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.11-1.21 g/mL
Molecules identified in the study Protein
mRNA
miRNA
Methods used in the study Mass spectrometry [MALDI TOF]
Western blotting
Microarray
miRCURY LNA Array
 Protein-protein interactions for Hsp90b1
  Protein Interactor ExoCarta ID Identification method PubMed Species
No interactions are found.
 Pathways in which Hsp90b1 is involved
PathwayEvidenceSource
Scavenging by Class A Receptors IEA Reactome
Trafficking and processing of endosomal TLR IEA Reactome





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