Gene description for Ago1
Gene name argonaute RISC catalytic subunit 1
Gene symbol Ago1
Other names/aliases Eif2c1
Species Mus musculus
 Database cross references - Ago1
ExoCarta ExoCarta_236511
Entrez Gene 236511
UniProt Q8CJG1  
 Ago1 identified in exosomes derived from the following tissue/cell type
Mast cells 17486113    
Neural stem cells 25242146    
 Gene ontology annotations for Ago1
Molecular Function
    RNA polymerase II core binding GO:0000993 ISO
    RNA binding GO:0003723 ISO
    RNA polymerase II core promoter proximal region sequence-specific DNA binding GO:0000978 IDA
    double-stranded RNA binding GO:0003725 ISO
    nucleic acid binding GO:0003676 IEA
    core promoter binding GO:0001047 ISO
    protein binding GO:0005515 IPI
    single-stranded RNA binding GO:0003727 ISO
    pre-miRNA binding GO:0070883 ISO
    poly(A) RNA binding GO:0044822 ISO
    transcription regulatory region DNA binding GO:0044212 IMP
    miRNA binding GO:0035198 ISO
Biological Process
    production of miRNAs involved in gene silencing by miRNA GO:0035196 ISO
    pre-miRNA processing GO:0031054 ISO
    transcription, DNA-templated GO:0006351 IEA
    miRNA loading onto RISC involved in gene silencing by miRNA GO:0035280 ISO
    regulation of transcription, DNA-templated GO:0006355 IEA
    miRNA metabolic process GO:0010586 IDA
    gene silencing by RNA GO:0031047 IEA
    positive regulation of transcription from RNA polymerase II promoter GO:0045944 ISO
    regulation of translation GO:0006417 IEA
    negative regulation of translation involved in gene silencing by miRNA GO:0035278 ISO
    RNA secondary structure unwinding GO:0010501 ISO
    nuclear-transcribed mRNA catabolic process GO:0000956 ISO
    positive regulation of gene expression GO:0010628 IMP
Subcellular Localization
    ribonucleoprotein complex GO:0030529 ISO
    cytosol GO:0005829 IDA
    micro-ribonucleoprotein complex GO:0035068 ISO
    cytoplasmic mRNA processing body GO:0000932 IDA
    RISC-loading complex GO:0070578 ISO
    polysome GO:0005844 ISO
    nucleus GO:0005634 IDA
    RISC complex GO:0016442 ISO
    cytoplasm GO:0005737 ISO
 Experiment description of studies that identified Ago1 in exosomes
1
Experiment ID 15
ISEV standards
✔
EM
EV Biophysical techniques
✘ EV Cytosolic markers
✔
CD63
EV Membrane markers
✘ EV Negative markers
✘ EV Particle analysis
Identified molecule mrna
Identification method Microarray
PubMed ID 17486113    
Organism Mus musculus
Homo sapiens
Experiment description Exosome-mediated transfer of mRNAs and microRNAs is a novel mechanism of genetic exchange between cells.
Authors Valadi H, Ekstrom K, Bossios A, Sjostrand M, Lee JJ, Lotvall JO
Journal name NCB
Publication year 2007
Sample Mast cells
Sample name MC9
Bone marrow-derived mast cells
HMC-1
Isolation/purification methods Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.11-1.21 g/mL
Molecules identified in the study Protein
mRNA
miRNA
Methods used in the study Mass spectrometry [MALDI TOF]
Western blotting
Microarray
miRCURY LNA Array
2
Experiment ID 262
ISEV standards
✔
EM
EV Biophysical techniques
✔
Alix|TSG101|HSP70|HSP90
EV Cytosolic markers
✔
CD63|CD9|
EV Membrane markers
✘ EV Negative markers
✔
Y
EV Particle analysis
Identified molecule protein
Identification method Western blotting
PubMed ID 25242146    
Organism Mus musculus
Experiment description Extracellular Vesicles from Neural Stem Cells Transfer IFN-γ via Ifngr1 to Activate Stat1 Signaling in Target Cells
Authors Chiara Cossetti, Nunzio Iraci, Tim R. Mercer, Tommaso Leonardi, Emanuele Alpi, Denise Drago, Clara Alfaro-Cervello, Harpreet K. Saini, Matthew P. Davis, Julia Schaeffer, Beatriz Vega, Matilde Stefanini, CongJian Zhao, Werner Muller, Jose Manuel Garcia-Verdugo, Suresh Mathivanan, Angela Bachi, Anton J. Enright, John S. Mattick, Stefano Pluchino
Journal name Molecular Cell
Publication year 2014
Sample Neural stem cells
Sample name NPCs
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.20 g/mL
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
Microarray
 Protein-protein interactions for Ago1
  Protein Interactor ExoCarta ID Identification method PubMed Species
No interactions are found.



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