Gene description for Hnrnpu
Gene name heterogeneous nuclear ribonucleoprotein U
Gene symbol Hnrnpu
Other names/aliases AA408410
AI256620
AL024194
AL024437
AW557595
C86794
Hnrpu
SAFA
Sp120
hnRNP U
Species Mus musculus
 Database cross references - Hnrnpu
ExoCarta ExoCarta_51810
Entrez Gene 51810
UniProt Q8VEK3  
 Hnrnpu identified in exosomes derived from the following tissue/cell type
Macrophages 23658846    
Macrophages 23658846    
Macrophages 23658846    
Neural stem cells 25242146    
 Gene ontology annotations for Hnrnpu
Molecular Function
    RNA binding GO:0003723 IEA
    DNA binding GO:0003677 IEA
    core promoter binding GO:0001047 IDA
    poly(A) RNA binding GO:0044822 ISO
    ATP binding GO:0005524 IEA
    nucleotide binding GO:0000166 IEA
    protein binding GO:0005515 IPI
Biological Process
    CRD-mediated mRNA stabilization GO:0070934 ISO
    osteoblast differentiation GO:0001649 ISO
    mRNA processing GO:0006397 IEA
    rhythmic process GO:0048511 IEA
    circadian regulation of gene expression GO:0032922 IDA
    RNA splicing GO:0008380 IEA
Subcellular Localization
    nucleus GO:0005634 ISO
    nucleoplasm GO:0005654 ISO
    CRD-mediated mRNA stability complex GO:0070937 ISO
    cytoplasmic ribonucleoprotein granule GO:0036464 ISO
    spliceosomal complex GO:0005681 IEA
    catalytic step 2 spliceosome GO:0071013 ISO
    ribonucleoprotein complex GO:0030529 ISO
    membrane GO:0016020 ISO
    cytoplasm GO:0005737 IEA
 Experiment description of studies that identified Hnrnpu in exosomes
1
Experiment ID 214
ISEV standards
EM
EV Biophysical techniques
EV Cytosolic markers
EV Membrane markers
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23658846    
Organism Mus musculus
Experiment description Immunomodulatory impact of leishmania-induced macrophage exosomes: a comparative proteomic and functional analysis.
Authors Hassani K, Olivier M.
Journal name PLoS Negl Trop Dis
Publication year 2013
Sample Macrophages
Sample name Leishmania-infected-Macrophage (J774A.1)
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Filtration
Protease inhibitors
Sucrose density gradient
Filtration
Ultracentrifugation
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
2
Experiment ID 215
ISEV standards
EM
EV Biophysical techniques
EV Cytosolic markers
EV Membrane markers
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23658846    
Organism Mus musculus
Experiment description Immunomodulatory impact of leishmania-induced macrophage exosomes: a comparative proteomic and functional analysis.
Authors Hassani K, Olivier M.
Journal name PLoS Negl Trop Dis
Publication year 2013
Sample Macrophages
Sample name LPS-treated-Macrophage (J774A.1)
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Filtration
Protease inhibitors
Sucrose density gradient
Filtration
Ultracentrifugation
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
3
Experiment ID 216
ISEV standards
EM
EV Biophysical techniques
EV Cytosolic markers
EV Membrane markers
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23658846    
Organism Mus musculus
Experiment description Immunomodulatory impact of leishmania-induced macrophage exosomes: a comparative proteomic and functional analysis.
Authors Hassani K, Olivier M.
Journal name PLoS Negl Trop Dis
Publication year 2013
Sample Macrophages
Sample name Normal-Macrophage (J774A.1)
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Filtration
Protease inhibitors
Sucrose density gradient
Filtration
Ultracentrifugation
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
4
Experiment ID 263
ISEV standards
EV Biophysical techniques
EV Cytosolic markers
CD63|CD9
EV Membrane markers
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25242146    
Organism Mus musculus
Experiment description Extracellular Vesicles from Neural Stem Cells Transfer IFN-γ via Ifngr1 to Activate Stat1 Signaling in Target Cells
Authors Chiara Cossetti, Nunzio Iraci, Tim R. Mercer, Tommaso Leonardi, Emanuele Alpi, Denise Drago, Clara Alfaro-Cervello, Harpreet K. Saini, Matthew P. Davis, Julia Schaeffer, Beatriz Vega, Matilde Stefanini, CongJian Zhao, Werner Muller, Jose Manuel Garcia-Verdugo, Suresh Mathivanan, Angela Bachi, Anton J. Enright, John S. Mattick, Stefano Pluchino
Journal name Molecular Cell
Publication year 2014
Sample Neural stem cells
Sample name NPCs - Th1 treated
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.20 g/mL
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for Hnrnpu
  Protein Interactor ExoCarta ID Identification method PubMed Species
No interactions are found.
 Pathways in which Hnrnpu is involved
PathwayEvidenceSource
mRNA Splicing - Major Pathway IEA Reactome
Processing of Capped Intron-Containing Pre-mRNA IEA Reactome





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