Gene description for Cdc5l
Gene name cell division cycle 5-like (S. pombe)
Gene symbol Cdc5l
Other names/aliases 1200002I02Rik
AA408004
PCDC5RP
Species Mus musculus
 Database cross references - Cdc5l
ExoCarta ExoCarta_71702
Vesiclepedia VP_71702
Entrez Gene 71702
UniProt Q6A068  
 Cdc5l identified in exosomes derived from the following tissue/cell type
Mast cells 17486113    
 Gene ontology annotations for Cdc5l
Molecular Function
    RNA polymerase II transcription regulatory region sequence-specific DNA binding GO:0000977 IBA
    RNA polymerase II transcription regulatory region sequence-specific DNA binding GO:0000977 ISO
    DNA-binding transcription factor activity, RNA polymerase II-specific GO:0000981 IBA
    transcription corepressor binding GO:0001222 ISO
    DNA-binding transcription activator activity, RNA polymerase II-specific GO:0001228 ISO
    DNA binding GO:0003677 IEA
    RNA binding GO:0003723 IEA
    protein phosphatase 1 binding GO:0008157 ISO
    protein kinase binding GO:0019901 ISO
    identical protein binding GO:0042802 ISO
    leucine zipper domain binding GO:0043522 ISO
    WD40-repeat domain binding GO:0071987 ISO
    WD40-repeat domain binding GO:0071987 ISS
Biological Process
    DNA damage checkpoint signaling GO:0000077 ISO
    DNA damage checkpoint signaling GO:0000077 ISS
    mRNA splicing, via spliceosome GO:0000398 IBA
    mRNA splicing, via spliceosome GO:0000398 ISO
    mRNA splicing, via spliceosome GO:0000398 ISS
    mRNA splicing, via spliceosome GO:0000398 NAS
    DNA repair GO:0006281 IEA
    regulation of transcription by RNA polymerase II GO:0006357 IBA
    positive regulation of transcription by RNA polymerase II GO:0045944 ISO
    cellular response to wortmannin GO:1904568 ISO
    cellular response to nerve growth factor stimulus GO:1990090 ISO
Subcellular Localization
    Prp19 complex GO:0000974 IBA
    Prp19 complex GO:0000974 ISO
    Prp19 complex GO:0000974 ISS
    nucleus GO:0005634 ISO
    nucleus GO:0005634 ISS
    nucleoplasm GO:0005654 ISO
    DNA replication factor A complex GO:0005662 ISO
    DNA replication factor A complex GO:0005662 ISS
    spliceosomal complex GO:0005681 IBA
    spliceosomal complex GO:0005681 ISO
    cytoplasm GO:0005737 IEA
    cytoplasm GO:0005737 ISO
    nuclear speck GO:0016607 IEA
    nuclear speck GO:0016607 ISO
    protein-DNA complex GO:0032993 ISO
    perinuclear region of cytoplasm GO:0048471 ISO
    U2-type catalytic step 2 spliceosome GO:0071007 ISO
    U2-type catalytic step 2 spliceosome GO:0071007 ISS
    catalytic step 2 spliceosome GO:0071013 ISO
 Experiment description of studies that identified Cdc5l in exosomes
1
Experiment ID 15
MISEV standards
EM
Biophysical techniques
CD63
Enriched markers
Negative markers
Particle analysis
Identified molecule mrna
Identification method Microarray
PubMed ID 17486113    
Organism Mus musculus
Homo sapiens
Experiment description Exosome-mediated transfer of mRNAs and microRNAs is a novel mechanism of genetic exchange between cells.
Authors "Valadi H, Ekstrom K, Bossios A, Sjostrand M, Lee JJ, Lotvall JO"
Journal name NCB
Publication year 2007
Sample Mast cells
Sample name MC9
Bone marrow-derived mast cells
HMC-1
Isolation/purification methods Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.11-1.21 g/mL
Molecules identified in the study Protein
mRNA
miRNA
Methods used in the study Mass spectrometry [MALDI TOF]
Western blotting
Microarray
miRCURY LNA Array
 Protein-protein interactions for Cdc5l
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 CCDC12  
Affinity Capture-MS Homo sapiens
2 Sf3a3 75062
Co-fractionation Mus musculus
3 Fxr1 14359
Affinity Capture-MS Mus musculus
4 Tnik  
Affinity Capture-MS Mus musculus
5 Snrnp200 320632
Co-fractionation Mus musculus
6 Sf3b3 101943
Co-fractionation Mus musculus
7 Gpatch1  
Co-fractionation Mus musculus
8 Prpf40a  
Co-fractionation Mus musculus
9 BCAS2 10286
Affinity Capture-MS Homo sapiens
10 Hnrnpc 15381
Co-fractionation Mus musculus
11 Thoc1  
Co-fractionation Mus musculus
12 Prpf19 28000
Co-fractionation Mus musculus
13 Prpf8 192159
Co-fractionation Mus musculus
14 PRPF8 10594
Affinity Capture-MS Homo sapiens
15 Smu1  
Co-fractionation Mus musculus
16 Alyref  
Co-fractionation Mus musculus
17 PLRG1 5356
Affinity Capture-MS Homo sapiens
18 Hnrnpu 51810
Co-fractionation Mus musculus
19 Fmr1  
Affinity Capture-MS Mus musculus
20 CTNNBL1 56259
Affinity Capture-MS Homo sapiens
21 Foxp3  
Affinity Capture-MS Mus musculus
22 Kctd13  
Affinity Capture-MS Mus musculus
23 XAB2 56949
Affinity Capture-MS Homo sapiens
24 Snrpd2 107686
Co-fractionation Mus musculus
25 Frg1  
Co-fractionation Mus musculus
26 Bzw1 66882
Co-fractionation Mus musculus
27 CALM1 801
Reconstituted Complex Homo sapiens
28 Rpa1 68275
Co-fractionation Mus musculus
29 AQR 9716
Affinity Capture-MS Homo sapiens
30 Snrnp27  
Co-fractionation Mus musculus
31 Ddx17 67040
Co-fractionation Mus musculus
32 CWC15  
Affinity Capture-MS Homo sapiens
33 Cwc22  
Co-fractionation Mus musculus
34 Lsm7 66094
Co-fractionation Mus musculus
35 Prpf3 70767
Co-fractionation Mus musculus
36 Pnn  
Co-fractionation Mus musculus
37 Cwc15  
Co-fractionation Mus musculus
38 Fzr1  
Affinity Capture-MS Mus musculus
39 SNRNP200 23020
Affinity Capture-MS Homo sapiens
40 Thoc2  
Co-fractionation Mus musculus
41 Plrg1  
Co-fractionation Mus musculus
42 Ctnnbl1  
Co-fractionation Mus musculus
43 CRNKL1 51340
Affinity Capture-MS Homo sapiens
44 Sf3b1 81898
Co-fractionation Mus musculus
45 Spen  
Co-fractionation Mus musculus
46 2310036O22Rik  
Co-fractionation Mus musculus
47 SNRPD1 6632
Affinity Capture-MS Homo sapiens
48 Snw1  
Co-fractionation Mus musculus
49 PPIE 10450
Affinity Capture-MS Homo sapiens
50 Srrm2  
Co-fractionation Mus musculus
51 Ppwd1  
Co-fractionation Mus musculus
52 Sfpq 71514
Co-fractionation Mus musculus
53 Hnrnpm 76936
Co-fractionation Mus musculus
54 Snrpa1 68981
Co-fractionation Mus musculus
55 PRPF19 27339
Affinity Capture-MS Homo sapiens
56 Ppil3  
Co-fractionation Mus musculus
57 Ddx42 72047
Co-fractionation Mus musculus
58 Eed  
Affinity Capture-MS Mus musculus
59 Syncrip 56403
Co-fractionation Mus musculus
60 Cwc27  
Co-fractionation Mus musculus
61 EFTUD2 9343
Affinity Capture-MS Homo sapiens
62 Skiv2l2  
Co-fractionation Mus musculus
63 Pcbd2  
Co-fractionation Mus musculus
64 Rbm39 170791
Co-fractionation Mus musculus
65 Otx2  
Affinity Capture-MS Mus musculus
66 Fancd2  
Affinity Capture-MS Mus musculus
67 CDC5L 988
Affinity Capture-MS Homo sapiens
68 Paxbp1  
Co-fractionation Mus musculus
69 Ppie  
Co-fractionation Mus musculus
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