Gene description for PLRG1
Gene name pleiotropic regulator 1
Gene symbol PLRG1
Other names/aliases Cwc1
PRL1
PRP46
PRPF46
TANGO4
Species Homo sapiens
 Database cross references - PLRG1
ExoCarta ExoCarta_5356
Vesiclepedia VP_5356
Entrez Gene 5356
HGNC 9089
MIM 605961
UniProt O43660  
 PLRG1 identified in exosomes derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Urine 22418980    
Urine 22418980    
 Gene ontology annotations for PLRG1
Molecular Function
    protein binding GO:0005515 IPI
Biological Process
    mRNA splicing, via spliceosome GO:0000398 IBA
    mRNA splicing, via spliceosome GO:0000398 IC
    mRNA splicing, via spliceosome GO:0000398 IDA
    mRNA splicing, via spliceosome GO:0000398 NAS
    protein localization to nucleus GO:0034504 IEA
    positive regulation of G1/S transition of mitotic cell cycle GO:1900087 IEA
Subcellular Localization
    Prp19 complex GO:0000974 IBA
    Prp19 complex GO:0000974 IPI
    fibrillar center GO:0001650 IDA
    nucleus GO:0005634 IDA
    nucleoplasm GO:0005654 IDA
    nucleoplasm GO:0005654 TAS
    DNA replication factor A complex GO:0005662 IDA
    spliceosomal complex GO:0005681 IPI
    nuclear speck GO:0016607 IDA
    nuclear membrane GO:0031965 IDA
    U2-type catalytic step 2 spliceosome GO:0071007 IDA
    catalytic step 2 spliceosome GO:0071013 IBA
    catalytic step 2 spliceosome GO:0071013 IDA
 Experiment description of studies that identified PLRG1 in exosomes
1
Experiment ID 489
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
5
Experiment ID 196
MISEV standards
✔
EM
Biophysical techniques
✔
Alix|TSG101|HSP70|CD9
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 22418980    
Organism Homo sapiens
Experiment description A multiplex quantitative proteomics strategy for protein biomarker studies in urinary exosomes.
Authors "Raj DA, Fiume I, Capasso G, Pocsfalvi G."
Journal name Kidney Int
Publication year 2012
Sample Urine
Sample name Urine - Normal high density
Isolation/purification methods Differential centrifugation
Sucrose cushion
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
6
Experiment ID 197
MISEV standards
✔
EM
Biophysical techniques
✔
Alix|TSG101|HSP70|CD9
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 22418980    
Organism Homo sapiens
Experiment description A multiplex quantitative proteomics strategy for protein biomarker studies in urinary exosomes.
Authors "Raj DA, Fiume I, Capasso G, Pocsfalvi G."
Journal name Kidney Int
Publication year 2012
Sample Urine
Sample name Urine - Normal low density
Isolation/purification methods Differential centrifugation
Sucrose cushion
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for PLRG1
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 UBE2H 7328
Affinity Capture-MS Homo sapiens
2 SNRPA 6626
Affinity Capture-MS Homo sapiens
Co-fractionation Homo sapiens
3 CCDC94  
Affinity Capture-MS Homo sapiens
Co-fractionation Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
4 Smek1  
Affinity Capture-MS Mus musculus
5 Prpf8 192159
Affinity Capture-MS Mus musculus
6 KPNA1 3836
Affinity Capture-Western Homo sapiens
7 PCF11 51585
Co-fractionation Homo sapiens
8 HIST1H4A 8359
Affinity Capture-MS Homo sapiens
9 PPIE 10450
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
10 FMR1 2332
Affinity Capture-MS Homo sapiens
11 RPA2 6118
Co-fractionation Homo sapiens
12 CWF19L2  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
13 CTNNBL1 56259
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
14 DHX8 1659
Proximity Label-MS Homo sapiens
15 Ccdc12  
Affinity Capture-MS Mus musculus
16 PRPF3  
Co-fractionation Homo sapiens
17 KPNA2 3838
Affinity Capture-Western Homo sapiens
18 AAR2 25980
Affinity Capture-MS Homo sapiens
19 AQR 9716
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
20 SYF2  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
21 SRSF2 6427
Co-fractionation Homo sapiens
22 SNRPF 6636
Affinity Capture-MS Homo sapiens
23 GCN1L1 10985
Co-fractionation Homo sapiens
24 SNRPD2 6633
Co-fractionation Homo sapiens
Co-fractionation Homo sapiens
25 APP 351
Reconstituted Complex Homo sapiens
Reconstituted Complex Homo sapiens
26 SF3B2 10992
Co-fractionation Homo sapiens
27 ERCC5  
Co-fractionation Homo sapiens
28 GSK3A 2931
Affinity Capture-MS Homo sapiens
29 USB1  
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
30 TOP2A 7153
Co-fractionation Homo sapiens
31 CRNKL1 51340
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
32 GPATCH1  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
33 MRPL40 64976
Co-fractionation Homo sapiens
34 Snw1  
Affinity Capture-MS Mus musculus
Affinity Capture-MS Mus musculus
35 SF3B1 23451
Two-hybrid Homo sapiens
Affinity Capture-MS Homo sapiens
Two-hybrid Homo sapiens
Co-fractionation Homo sapiens
36 MDC1  
Affinity Capture-MS Homo sapiens
37 SNRPC 6631
Affinity Capture-MS Homo sapiens
38 DLST 1743
Affinity Capture-MS Homo sapiens
39 SF3A2 8175
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Co-fractionation Homo sapiens
40 SF3B3 23450
Co-fractionation Homo sapiens
41 SNRPD3 6634
Co-fractionation Homo sapiens
42 UCHL5 51377
Co-fractionation Homo sapiens
43 MECP2 4204
Affinity Capture-MS Homo sapiens
44 MYCN  
Affinity Capture-MS Homo sapiens
45 PABPC4 8761
Co-fractionation Homo sapiens
46 RBM14 10432
Co-fractionation Homo sapiens
47 KIF14 9928
Affinity Capture-MS Homo sapiens
48 DYNC1H1 1778
Co-fractionation Homo sapiens
49 HSPA8 3312
Co-fractionation Homo sapiens
50 ZCRB1  
Co-fractionation Homo sapiens
51 RBMX 27316
Co-fractionation Homo sapiens
52 Bcas2  
Affinity Capture-MS Mus musculus
53 E2F4  
Affinity Capture-MS Homo sapiens
54 PPP1CA 5499
Co-fractionation Homo sapiens
55 PRPF8 10594
Co-fractionation Homo sapiens
Proximity Label-MS Homo sapiens
56 RPL9 6133
Co-fractionation Homo sapiens
57 PARK2  
Affinity Capture-MS Homo sapiens
58 RBM6 10180
Affinity Capture-MS Homo sapiens
59 XPA  
Co-fractionation Homo sapiens
60 Crnkl1  
Affinity Capture-MS Mus musculus
61 BACH1 571
Affinity Capture-MS Homo sapiens
62 CPVL 54504
Affinity Capture-MS Homo sapiens
63 SNRPB 6628
Affinity Capture-MS Homo sapiens
64 PABPC1 26986
Co-fractionation Homo sapiens
65 MKRN2 23609
Affinity Capture-MS Homo sapiens
66 SNRPD1 6632
Co-fractionation Homo sapiens
67 NTRK1 4914
Affinity Capture-MS Homo sapiens
68 U2AF2 11338
Affinity Capture-Western Homo sapiens
69 HECTD1 25831
Affinity Capture-MS Homo sapiens
70 MAGOH 4116
Affinity Capture-MS Homo sapiens
71 CEBPA  
Protein-peptide Homo sapiens
72 SF3A3 10946
Co-fractionation Homo sapiens
73 BZW1 9689
Co-fractionation Homo sapiens
74 SNRPB2 6629
Affinity Capture-MS Homo sapiens
75 SCARNA22  
Affinity Capture-RNA Homo sapiens
76 RNF40 9810
Affinity Capture-MS Homo sapiens
77 RBM39 9584
Affinity Capture-MS Homo sapiens
Co-fractionation Homo sapiens
78 USP42  
Affinity Capture-MS Homo sapiens
79 PRPF4B 8899
Affinity Capture-MS Homo sapiens
80 FANCD2  
Affinity Capture-MS Homo sapiens
81 CUL3 8452
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
82 XPO1 7514
Affinity Capture-MS Homo sapiens
83 RC3H2  
Affinity Capture-MS Homo sapiens
84 DHX35  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
85 CD2BP2 10421
Affinity Capture-MS Homo sapiens
86 ZSCAN1  
Two-hybrid Homo sapiens
87 SRSF1 6426
Co-fractionation Homo sapiens
88 MX1 4599
Two-hybrid Homo sapiens
89 XAB2 56949
Co-fractionation Homo sapiens
Co-fractionation Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
90 DNAJC17  
Proximity Label-MS Homo sapiens
91 FUS 2521
Affinity Capture-MS Homo sapiens
92 TSSC4  
Affinity Capture-MS Homo sapiens
93 SUZ12  
Affinity Capture-MS Homo sapiens
94 AP2M1 1173
Co-fractionation Homo sapiens
95 SF3A1 10291
Affinity Capture-MS Homo sapiens
Co-fractionation Homo sapiens
Co-fractionation Homo sapiens
96 TADA2A  
Affinity Capture-MS Homo sapiens
97 PRPF19 27339
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Co-fractionation Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Co-fractionation Homo sapiens
98 MYC  
Affinity Capture-MS Homo sapiens
99 RPA3 6119
Proximity Label-MS Homo sapiens
Co-fractionation Homo sapiens
100 EGFR 1956
Negative Genetic Homo sapiens
101 CSNK2A2 1459
Affinity Capture-MS Homo sapiens
102 TOP1 7150
Affinity Capture-MS Homo sapiens
103 CHD8 57680
Affinity Capture-MS Homo sapiens
104 DDRGK1 65992
Affinity Capture-MS Homo sapiens
105 BMI1  
Affinity Capture-MS Homo sapiens
106 PRKDC 5591
Co-fractionation Homo sapiens
107 ISY1-RAB43 100534599
Co-fractionation Homo sapiens
108 ISY1 57461
Co-fractionation Homo sapiens
Co-fractionation Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
109 PLEKHA4 57664
Affinity Capture-MS Homo sapiens
110 EAPP  
Affinity Capture-MS Homo sapiens
111 LINS  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
112 BCAS2 10286
Affinity Capture-MS Homo sapiens
Co-fractionation Homo sapiens
Co-purification Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Co-fractionation Homo sapiens
Co-fractionation Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
113 PDIA5 10954
Co-fractionation Homo sapiens
114 SRRM1 10250
Affinity Capture-MS Homo sapiens
Co-fractionation Homo sapiens
115 ILF2 3608
Co-fractionation Homo sapiens
116 Cdc5l 71702
Affinity Capture-MS Mus musculus
117 SNRPA1 6627
Co-fractionation Homo sapiens
Co-fractionation Homo sapiens
118 SF3B4 10262
Co-fractionation Homo sapiens
119 RPA1 6117
Co-fractionation Homo sapiens
120 MEN1 4221
Affinity Capture-MS Homo sapiens
121 PDHA1 5160
Affinity Capture-MS Homo sapiens
122 HNRNPM 4670
Affinity Capture-Western Homo sapiens
FRET Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
123 HDAC1 3065
Affinity Capture-MS Homo sapiens
124 EIF4A3 9775
Affinity Capture-MS Homo sapiens
125 CWC15  
Co-fractionation Homo sapiens
Co-fractionation Homo sapiens
126 ESR1  
Affinity Capture-MS Homo sapiens
127 UFL1 23376
Affinity Capture-MS Homo sapiens
128 ARHGAP36  
Affinity Capture-MS Homo sapiens
129 FLNC 2318
Co-fractionation Homo sapiens
130 PRPF4 9128
Co-fractionation Homo sapiens
131 SNW1 22938
Affinity Capture-MS Homo sapiens
Co-fractionation Homo sapiens
132 KRAS 3845
Negative Genetic Homo sapiens
133 ARHGAP23 57636
Affinity Capture-MS Homo sapiens
134 C9orf72  
Affinity Capture-MS Homo sapiens
135 RC3H1 149041
Affinity Capture-MS Homo sapiens
136 SSRP1 6749
Affinity Capture-MS Homo sapiens
137 PRMT1 3276
Affinity Capture-MS Homo sapiens
138 CPSF6 11052
Affinity Capture-MS Homo sapiens
139 EP300 2033
Affinity Capture-MS Homo sapiens
140 CDC5L 988
Protein-peptide Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-MS Homo sapiens
Co-purification Homo sapiens
Co-fractionation Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Reconstituted Complex Homo sapiens
Far Western Homo sapiens
FRET Homo sapiens
Co-fractionation Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Co-fractionation Homo sapiens
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