Gene description for PDIA5
Gene name protein disulfide isomerase family A, member 5
Gene symbol PDIA5
Other names/aliases PDIR
Species Homo sapiens
 Database cross references - PDIA5
ExoCarta ExoCarta_10954
Vesiclepedia VP_10954
Entrez Gene 10954
HGNC 24811
UniProt Q14554  
 PDIA5 identified in exosomes derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Mesenchymal stem cells 36408942    
Ovarian cancer cells 23333927    
Ovarian cancer cells 23333927    
Platelets 25332113    
Platelets 25332113    
Platelets 25332113    
 Gene ontology annotations for PDIA5
Molecular Function
    protein disulfide isomerase activity GO:0003756 IBA
    protein disulfide isomerase activity GO:0003756 IDA
    protein disulfide isomerase activity GO:0003756 TAS
    protein binding GO:0005515 IPI
    protein-disulfide reductase activity GO:0015035 IDA
    oxidoreductase activity GO:0016491 TAS
Biological Process
    protein folding GO:0006457 IBA
    protein folding GO:0006457 IDA
    protein folding GO:0006457 TAS
    electron transport chain GO:0022900 TAS
Subcellular Localization
    endoplasmic reticulum GO:0005783 IBA
    endoplasmic reticulum lumen GO:0005788 TAS
    endoplasmic reticulum membrane GO:0005789 TAS
 Experiment description of studies that identified PDIA5 in exosomes
1
Experiment ID 489
MISEV standards
EM
Biophysical techniques
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
Canx
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
EM
Biophysical techniques
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
Canx
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
EM
Biophysical techniques
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
Canx
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
EM
Biophysical techniques
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
Canx
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
5
Experiment ID 488
MISEV standards
EM
Biophysical techniques
CD9|CD81|CD63|GAPDH|SDCBP|LAMP1|TFRC|UCHL1|FLOT2|LAMP2|FLOT1|ICAM1|RAB5B|CD151|RAB35|TSG101|RAB5A|CD82
Enriched markers
CANX
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Homo sapiens
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Mesenchymal stem cells
Sample name UCMSC
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
6
Experiment ID 211
MISEV standards
EM
Biophysical techniques
TSG101|Alix|EpCAM|TFRC
Enriched markers
cytochrome c|GOLGA2
Negative markers
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23333927    
Organism Homo sapiens
Experiment description Characterization and proteomic analysis of ovarian cancer-derived exosomes.
Authors "Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K."
Journal name J Proteomics
Publication year 2013
Sample Ovarian cancer cells
Sample name IGROV1
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.09-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
7
Experiment ID 212
MISEV standards
CEM
Biophysical techniques
TSG101|Alix|EpCAM|TFRC
Enriched markers
Cytochrome C|GOLGA2
Negative markers
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23333927    
Organism Homo sapiens
Experiment description Characterization and proteomic analysis of ovarian cancer-derived exosomes.
Authors "Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K."
Journal name J Proteomics
Publication year 2013
Sample Ovarian cancer cells
Sample name OVCAR-3
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.09-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
8
Experiment ID 231
MISEV standards
Biophysical techniques
Alix|CD63|CD9
Enriched markers
Negative markers
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25332113    
Organism Homo sapiens
Experiment description Lipidomic and proteomic characterization of platelet extracellular vesicle subfractions from senescent platelets
Authors "Pienimaeki-Roemer A, Kuhlmann K, Bottcher A, Konovalova T, Black A, Orso E, Liebisch G, Ahrens M, Eisenacher M, Meyer HE, Schmitz G."
Journal name Transfusion
Publication year 2015
Sample Platelets
Sample name PL-Exs - Rep 1
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Optiprep density gradient
Flotation density 1.12-1.15 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Western blotting
Mass spectrometry
9
Experiment ID 232
MISEV standards
Biophysical techniques
Enriched markers
Negative markers
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25332113    
Organism Homo sapiens
Experiment description Lipidomic and proteomic characterization of platelet extracellular vesicle subfractions from senescent platelets
Authors "Pienimaeki-Roemer A, Kuhlmann K, Bottcher A, Konovalova T, Black A, Orso E, Liebisch G, Ahrens M, Eisenacher M, Meyer HE, Schmitz G."
Journal name Transfusion
Publication year 2015
Sample Platelets
Sample name PL-Exs - Rep 2
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Optiprep density gradient
Flotation density 1.12-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
10
Experiment ID 233
MISEV standards
Biophysical techniques
Enriched markers
Negative markers
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25332113    
Organism Homo sapiens
Experiment description Lipidomic and proteomic characterization of platelet extracellular vesicle subfractions from senescent platelets
Authors "Pienimaeki-Roemer A, Kuhlmann K, Bottcher A, Konovalova T, Black A, Orso E, Liebisch G, Ahrens M, Eisenacher M, Meyer HE, Schmitz G."
Journal name Transfusion
Publication year 2015
Sample Platelets
Sample name PL-Exs - Rep 3
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Optiprep density gradient
Flotation density 1.12-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for PDIA5
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 KRTAP10-7  
Two-hybrid Homo sapiens
2 ATG16L1 55054
Affinity Capture-MS Homo sapiens
3 POLR2G 5436
Co-fractionation Homo sapiens
4 CCL3  
Affinity Capture-MS Homo sapiens
5 G0S2  
Affinity Capture-MS Homo sapiens
6 ARSA 410
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
7 HNRNPH1 3187
Affinity Capture-RNA Homo sapiens
8 GPIHBP1  
Affinity Capture-MS Homo sapiens
9 PSMD14 10213
Co-fractionation Homo sapiens
10 CCL22 6367
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
11 TOR1AIP1 26092
Affinity Capture-MS Homo sapiens
12 LAIR2  
Affinity Capture-MS Homo sapiens
13 UBQLN4 56893
Two-hybrid Homo sapiens
14 CCL14  
Affinity Capture-MS Homo sapiens
15 FAM20C 56975
Affinity Capture-MS Homo sapiens
16 CUTA 51596
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
17 TP53 7157
Synthetic Growth Defect Homo sapiens
18 CDC5L 988
Co-fractionation Homo sapiens
19 BIRC2  
Affinity Capture-MS Homo sapiens
20 CALR3  
Proximity Label-MS Homo sapiens
21 PDIA4 9601
Proximity Label-MS Homo sapiens
22 LCN9  
Affinity Capture-MS Homo sapiens
23 HPN  
Affinity Capture-MS Homo sapiens
24 MME 4311
Affinity Capture-MS Homo sapiens
25 PLRG1 5356
Co-fractionation Homo sapiens
26 KRTAP10-3  
Two-hybrid Homo sapiens
27 SAA1 6288
Affinity Capture-MS Homo sapiens
28 IFI30  
Affinity Capture-MS Homo sapiens
29 ACAD11 84129
Affinity Capture-MS Homo sapiens
30 COX16  
Affinity Capture-MS Homo sapiens
31 MBL2 4153
Affinity Capture-MS Homo sapiens
32 CNPY2 10330
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
33 MORF4L2  
Co-fractionation Homo sapiens
34 MDFI  
Two-hybrid Homo sapiens
35 PSMB4 5692
Affinity Capture-MS Homo sapiens
36 MECP2 4204
Affinity Capture-MS Homo sapiens
37 ADPGK 83440
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
38 OIT3 170392
Affinity Capture-MS Homo sapiens
39 NENF 29937
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
40 WFDC2  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
41 LRRC59 55379
Proximity Label-MS Homo sapiens
42 FUCA1 2517
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
43 GGH 8836
Affinity Capture-MS Homo sapiens
44 MUCL1  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
45 C1QTNF7  
Affinity Capture-MS Homo sapiens
46 NXF1 10482
Affinity Capture-RNA Homo sapiens
47 ANTXR1 84168
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
48 CCL18  
Affinity Capture-MS Homo sapiens
49 PRPF19 27339
Co-fractionation Homo sapiens
50 TGIF2LX  
Affinity Capture-MS Homo sapiens
51 IDS  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
52 MYDGF 56005
Affinity Capture-MS Homo sapiens
53 SDF2L1 23753
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
54 CGREF1 10669
Affinity Capture-MS Homo sapiens
55 LOC554223  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
56 OGT 8473
Reconstituted Complex Homo sapiens
57 SCGB2A2  
Affinity Capture-MS Homo sapiens
58 APOC3 345
Affinity Capture-MS Homo sapiens
59 CST6  
Affinity Capture-MS Homo sapiens
60 CRP  
Affinity Capture-MS Homo sapiens
61 EP300 2033
Affinity Capture-MS Homo sapiens
62 TRAF2 7186
Affinity Capture-MS Homo sapiens
63 TRDN 10345
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
64 NTM 50863
Affinity Capture-MS Homo sapiens
65 SPINK1 6690
Affinity Capture-MS Homo sapiens
66 LGMN 5641
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
67 PPP1R2 5504
Co-fractionation Homo sapiens
68 PATE1  
Affinity Capture-MS Homo sapiens
69 RNASET2  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
70 ST14 6768
Affinity Capture-MS Homo sapiens
71 SERPINI2  
Affinity Capture-MS Homo sapiens
72 TSR1 55720
Affinity Capture-MS Homo sapiens
73 ERP27  
Affinity Capture-MS Homo sapiens
74 PDGFRA 5156
Affinity Capture-MS Homo sapiens
75 SEC61B 10952
Proximity Label-MS Homo sapiens
76 LASP1 3927
Affinity Capture-MS Homo sapiens
77 DNAJB11 51726
Proximity Label-MS Homo sapiens
78 FUBP3 8939
Affinity Capture-MS Homo sapiens
79 CTRL  
Affinity Capture-MS Homo sapiens
80 PDIA6 10130
Affinity Capture-MS Homo sapiens
View the network image/svg+xml



Perform bioinformatics analysis of your extracellular vesicle data set using FunRich, a open access standalone tool. NEW UPDATED VERSION OF FunRich available for download (12/09/2016) from here