Gene description for ARSA
Gene name arylsulfatase A
Gene symbol ARSA
Other names/aliases MLD
Species Homo sapiens
 Database cross references - ARSA
ExoCarta ExoCarta_410
Vesiclepedia VP_410
Entrez Gene 410
HGNC 713
MIM 607574
UniProt P15289  
 ARSA identified in exosomes derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
 Gene ontology annotations for ARSA
Molecular Function
    arylsulfatase activity GO:0004065 IBA
    cerebroside-sulfatase activity GO:0004098 IEA
    calcium ion binding GO:0005509 IDA
    protein binding GO:0005515 IPI
    sulfuric ester hydrolase activity GO:0008484 IDA
Biological Process
    lipid metabolic process GO:0006629 IEA
Subcellular Localization
    extracellular region GO:0005576 TAS
    lysosome GO:0005764 TAS
    endoplasmic reticulum lumen GO:0005788 TAS
    azurophil granule lumen GO:0035578 TAS
    lysosomal lumen GO:0043202 TAS
    extracellular exosome GO:0070062 HDA
 Experiment description of studies that identified ARSA in exosomes
1
Experiment ID 489
MISEV standards
EM
Biophysical techniques
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
Canx
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
EM
Biophysical techniques
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
Canx
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
EM
Biophysical techniques
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
Canx
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
EM
Biophysical techniques
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
Canx
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
 Protein-protein interactions for ARSA
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 MESDC2 23184
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
2 NDUFV3 4731
Affinity Capture-MS Homo sapiens
3 ARSA 410
Co-crystal Structure Homo sapiens
Reconstituted Complex Homo sapiens
Co-crystal Structure Homo sapiens
Reconstituted Complex Homo sapiens
4 PPIC 5480
Two-hybrid Homo sapiens
5 HSPA5 3309
Affinity Capture-MS Homo sapiens
6 NAAA  
Affinity Capture-MS Homo sapiens
7 RAB25 57111
Affinity Capture-MS Homo sapiens
8 CLGN 1047
Affinity Capture-MS Homo sapiens
9 CNTNAP3  
Affinity Capture-MS Homo sapiens
10 PDIA5 10954
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
11 TNFRSF6B  
Affinity Capture-MS Homo sapiens
12 ART5  
Affinity Capture-MS Homo sapiens
13 CTU1 90353
Affinity Capture-MS Homo sapiens
14 SCGB1D1  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
15 KLK5 25818
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
16 NT5C1A  
Affinity Capture-MS Homo sapiens
17 NXPH4  
Affinity Capture-MS Homo sapiens
18 TRIP13 9319
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
19 KDELC1 79070
Affinity Capture-MS Homo sapiens
20 SH2D3A  
Affinity Capture-MS Homo sapiens
21 TMEM25  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
22 FBXO21  
Affinity Capture-MS Homo sapiens
23 CETN2 1069
Two-hybrid Homo sapiens
24 PLAUR 5329
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
25 TUBB8 347688
Affinity Capture-MS Homo sapiens
26 CALR 811
Affinity Capture-MS Homo sapiens
27 CUL3 8452
Affinity Capture-MS Homo sapiens
28 PLD4  
Affinity Capture-MS Homo sapiens
29 PPOX 5498
Affinity Capture-MS Homo sapiens
30 CNPY3 10695
Affinity Capture-MS Homo sapiens
31 MANF 7873
Affinity Capture-MS Homo sapiens
32 FAM132B  
Affinity Capture-MS Homo sapiens
33 PDIA4 9601
Affinity Capture-MS Homo sapiens
34 NCR3  
Affinity Capture-MS Homo sapiens
35 HSP90B1 7184
Affinity Capture-MS Homo sapiens
36 INSL5  
Affinity Capture-MS Homo sapiens
37 FBXL12 54850
Affinity Capture-MS Homo sapiens
38 SEMA3B 7869
Affinity Capture-MS Homo sapiens
39 LRPAP1 4043
Affinity Capture-MS Homo sapiens
40 SGSH 6448
Affinity Capture-MS Homo sapiens
41 DNASE2B  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
42 ADAMTS12 81792
Affinity Capture-MS Homo sapiens
43 CLEC4G  
Two-hybrid Homo sapiens
44 SUSD4  
Affinity Capture-MS Homo sapiens
45 GFPT2 9945
Affinity Capture-MS Homo sapiens
46 DEFB135  
Affinity Capture-MS Homo sapiens
47 CDC45  
Affinity Capture-MS Homo sapiens
48 PTPRK 5796
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
49 DNAJC3 5611
Affinity Capture-MS Homo sapiens
50 EDN3  
Affinity Capture-MS Homo sapiens
51 SUMF1 285362
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
52 GALNS 2588
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
53 HSPD1 3329
Affinity Capture-MS Homo sapiens
54 UPK1A 11045
Affinity Capture-MS Homo sapiens
55 DPEP2  
Affinity Capture-MS Homo sapiens
56 TRAV20  
Affinity Capture-MS Homo sapiens
57 GAS6 2621
Affinity Capture-MS Homo sapiens
58 WNT16  
Affinity Capture-MS Homo sapiens
59 ZBTB43  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
60 NFXL1 152518
Affinity Capture-MS Homo sapiens
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