Gene description for PLAUR
Gene name plasminogen activator, urokinase receptor
Gene symbol PLAUR
Other names/aliases CD87
U-PAR
UPAR
URKR
Species Homo sapiens
 Database cross references - PLAUR
ExoCarta ExoCarta_5329
Entrez Gene 5329
HGNC 9053
MIM 173391
UniProt Q03405  
 PLAUR identified in exosomes derived from the following tissue/cell type
Colorectal cancer cells 23161513    
Colorectal cancer cells 23161513    
Colorectal cancer cells 23161513    
Melanoma cells 25950383    
Melanoma cells 25950383    
Prostate cancer cells 25844599    
Prostate cancer cells 25844599    
 Gene ontology annotations for PLAUR
Molecular Function
    receptor activity GO:0004872 NAS
    receptor binding GO:0005102 IPI
    enzyme binding GO:0019899 IPI
    protein binding GO:0005515 IPI
    protein domain specific binding GO:0019904 IPI
    urokinase plasminogen activator receptor activity GO:0030377 NAS
Biological Process
    blood coagulation GO:0007596 TAS
    negative regulation of intrinsic apoptotic signaling pathway GO:2001243 IMP
    movement of cell or subcellular component GO:0006928 NAS
    negative regulation of apoptotic process GO:0043066 IMP
    chemotaxis GO:0006935 TAS
    negative regulation of cysteine-type endopeptidase activity involved in apoptotic signaling pathway GO:2001268 IMP
    positive regulation of protein phosphorylation GO:0001934 IMP
    positive regulation of epidermal growth factor receptor signaling pathway GO:0045742 IMP
    post-translational protein modification GO:0043687 TAS
    attachment of GPI anchor to protein GO:0016255 TAS
    signal transduction GO:0007165 TAS
    positive regulation of DNA binding GO:0043388 IDA
    cellular protein metabolic process GO:0044267 TAS
    positive regulation of release of cytochrome c from mitochondria GO:0090200 IMP
    regulation of proteolysis GO:0030162 NAS
    C-terminal protein lipidation GO:0006501 TAS
    urokinase plasminogen activator signaling pathway GO:0038195 NAS
    fibrinolysis GO:0042730 TAS
Subcellular Localization
    endoplasmic reticulum lumen GO:0005788 TAS
    integral component of membrane GO:0016021 NAS
    integral component of plasma membrane GO:0005887 IEA
    extracellular exosome GO:0070062 IDA
    anchored component of membrane GO:0031225 IEA
    endoplasmic reticulum membrane GO:0005789 TAS
    plasma membrane GO:0005886 TAS
    invadopodium membrane GO:0071438 IEA
    extrinsic component of membrane GO:0019898 TAS
    focal adhesion GO:0005925 IDA
 Experiment description of studies that identified PLAUR in exosomes
1
Experiment ID 207
ISEV standards
✔
EM
EV Biophysical techniques
✔
TSG101|HSP70
EV Cytosolic markers
✔
FLOT1
EV Membrane markers
✔
VDAC
EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23161513    
Organism Homo sapiens
Experiment description Proteomic analysis of exosomes from mutant KRAS colon cancer cells identifies intercellular transfer of mutant KRAS.
Authors Demory Beckler M, Higginbotham JN, Franklin JL, Ham AJ, Halvey PJ, Imasuen IE, Whitwell C, Li M, Liebler DC, Coffey RJ.
Journal name Mol Cell Proteomics
Publication year 2012
Sample Colorectal cancer cells
Sample name DKO-1
Isolation/purification methods Differential centrifugation
Filtration
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
2
Experiment ID 208
ISEV standards
✔
EM
EV Biophysical techniques
✔
TSG101|HSP70
EV Cytosolic markers
✔
FLOT1
EV Membrane markers
✔
VDAC
EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23161513    
Organism Homo sapiens
Experiment description Proteomic analysis of exosomes from mutant KRAS colon cancer cells identifies intercellular transfer of mutant KRAS.
Authors Demory Beckler M, Higginbotham JN, Franklin JL, Ham AJ, Halvey PJ, Imasuen IE, Whitwell C, Li M, Liebler DC, Coffey RJ.
Journal name Mol Cell Proteomics
Publication year 2012
Sample Colorectal cancer cells
Sample name Dks-8
Isolation/purification methods Differential centrifugation
Filtration
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
3
Experiment ID 209
ISEV standards
✘ EV Biophysical techniques
✔
TSG101|HSP70
EV Cytosolic markers
✔
FLOT1
EV Membrane markers
✔
VDAC
EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23161513    
Organism Homo sapiens
Experiment description Proteomic analysis of exosomes from mutant KRAS colon cancer cells identifies intercellular transfer of mutant KRAS.
Authors Demory Beckler M, Higginbotham JN, Franklin JL, Ham AJ, Halvey PJ, Imasuen IE, Whitwell C, Li M, Liebler DC, Coffey RJ.
Journal name Mol Cell Proteomics
Publication year 2012
Sample Colorectal cancer cells
Sample name DLD-1
Isolation/purification methods Differential centrifugation
Filtration
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
4
Experiment ID 259
ISEV standards
✔
EM
EV Biophysical techniques
✔
TSG101
EV Cytosolic markers
✔
FLOT1
EV Membrane markers
✘ EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25950383    
Organism Homo sapiens
Experiment description Proteome characterization of melanoma exosomes reveals a specific signature for metastatic cell lines
Authors Lazar I, Clement E, Ducoux-Petit M, Denat L, Soldan V, Dauvillier S, Balor S4, Burlet-Schiltz O1, Larue L, Muller C Nieto L
Journal name Pigment Cell Melanoma Res
Publication year 2015
Sample Melanoma cells
Sample name A375M
Isolation/purification methods Differential centrifugation
Unltracentrifugation
Sucrose density gradient
Flotation density 1.13 - 1.19 g/mL
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
5
Experiment ID 260
ISEV standards
✔
EM
EV Biophysical techniques
✔
TSG101
EV Cytosolic markers
✔
FLOT1|CD81
EV Membrane markers
✘ EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25950383    
Organism Homo sapiens
Experiment description Proteome characterization of melanoma exosomes reveals a specific signature for metastatic cell lines
Authors Lazar I, Clement E, Ducoux-Petit M, Denat L, Soldan V, Dauvillier S, Balor S4, Burlet-Schiltz O1, Larue L, Muller C Nieto L
Journal name Pigment Cell Melanoma Res
Publication year 2015
Sample Melanoma cells
Sample name 1205Lu
Isolation/purification methods Differential centrifugation
Unltracentrifugation
Sucrose density gradient
Flotation density 1.13 - 1.19 g/mL
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
6
Experiment ID 275
ISEV standards
✔
EM
EV Biophysical techniques
✔
TSG101|Alix|RAB5A
EV Cytosolic markers
✔
CD9|CD82|CD63|CD81
EV Membrane markers
✔
AIF
EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25844599    
Organism Homo sapiens
Experiment description Molecular profiling of prostate cancer derived exosomes may reveal a predictive signature for response to docetaxel.
Authors Kharaziha P, Chioureas D, Rutishauser D, Baltatzis G, Lennartsson L, Fonseca P, Azimi A, Hultenby K, Zubarev R, Ullen A, Yachnin J, Nilsson S, Panaretakis T.
Journal name Oncotarget
Publication year 2015
Sample Prostate cancer cells
Sample name DU145 - Docetaxel sensitive
Isolation/purification methods Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.12-1.19 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry/Flow cytometry/Western blotting
7
Experiment ID 274
ISEV standards
✔
EM
EV Biophysical techniques
✔
TSG101|Alix|RAB5A
EV Cytosolic markers
✔
CD9|CD82|CD63|CD81
EV Membrane markers
✔
AIF
EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25844599    
Organism Homo sapiens
Experiment description Molecular profiling of prostate cancer derived exosomes may reveal a predictive signature for response to docetaxel.
Authors Kharaziha P, Chioureas D, Rutishauser D, Baltatzis G, Lennartsson L, Fonseca P, Azimi A, Hultenby K, Zubarev R, Ullen A, Yachnin J, Nilsson S, Panaretakis T.
Journal name Oncotarget
Publication year 2015
Sample Prostate cancer cells
Sample name DU145 - Docetaxel resistant
Isolation/purification methods Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.18 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
Flow cytometry
Western blotting
 Protein-protein interactions for PLAUR
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 VTN 7448
Affinity Capture-MS Homo sapiens
2 ITGAM 3684
Reconstituted Complex Homo sapiens
3 LRP1 4035
Invitro Homo sapiens
4 PFN2 5217
Two-hybrid Homo sapiens
5 ITGB2 3689
Affinity Capture-Western Homo sapiens
6 FYN 2534
Affinity Capture-MS Homo sapiens
Invitro Homo sapiens
Invivo Homo sapiens
7 JAK1 3716
Invivo Homo sapiens
Invitro Homo sapiens
8 EEF1A1 1915
Two-hybrid Homo sapiens
9 HCK 3055
Affinity Capture-MS Homo sapiens
Invitro Homo sapiens
10 PLAU 5328
Affinity Capture-MS Homo sapiens
Invivo Homo sapiens
Invitro Homo sapiens
Reconstituted Complex Homo sapiens
11 FAP 2191
FRET Homo sapiens
12 IGF2R 3482
Reconstituted Complex Homo sapiens
13 KNG1 3827
Reconstituted Complex Homo sapiens
View the network image/svg+xml
 Pathways in which PLAUR is involved
PathwayEvidenceSource
Attachment of GPI anchor to uPAR TAS Reactome
Dissolution of Fibrin Clot TAS Reactome





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