Gene description for ITGAM
Gene name integrin, alpha M (complement component 3 receptor 3 subunit)
Gene symbol ITGAM
Other names/aliases CD11B
CR3A
MAC-1
MAC1A
MO1A
SLEB6
Species Homo sapiens
 Database cross references - ITGAM
ExoCarta ExoCarta_3684
Vesiclepedia VP_3684
Entrez Gene 3684
HGNC 6149
MIM 120980
UniProt P11215  
 ITGAM identified in exosomes derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Thymus 23844026    
 Gene ontology annotations for ITGAM
Molecular Function
    heparin binding GO:0008201 IEA
    protein heterodimerization activity GO:0046982 IEA
    opsonin binding GO:0001846 IEA
    protein binding GO:0005515 IPI
    glycoprotein binding GO:0001948 IPI
    heparan sulfate proteoglycan binding GO:0043395 IEA
    metal ion binding GO:0046872 IEA
Biological Process
    activated T cell proliferation GO:0050798 IEA
    blood coagulation GO:0007596 TAS
    cellular extravasation GO:0045123 IEA
    innate immune response GO:0045087 TAS
    leukocyte migration GO:0050900 TAS
    microglia development GO:0014005 IEA
    leukocyte migration involved in inflammatory response GO:0002523 IEA
    integrin-mediated signaling pathway GO:0007229 IEA
    toll-like receptor signaling pathway GO:0002224 TAS
    cell adhesion GO:0007155 TAS
    toll-like receptor 4 signaling pathway GO:0034142 TAS
    neutrophil chemotaxis GO:0030593 IEA
    extracellular matrix organization GO:0030198 TAS
    ectodermal cell differentiation GO:0010668 IEP
Subcellular Localization
    extracellular exosome GO:0070062 IDA
    cell surface GO:0009986 IDA
    plasma membrane GO:0005886 TAS
    integrin complex GO:0008305 IEA
    external side of plasma membrane GO:0009897 IEA
    nucleus GO:0005634 IEA
 Experiment description of studies that identified ITGAM in exosomes
1
Experiment ID 489
MISEV standards
✔
EM
EV Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
EV Enriched markers
✔
Canx
EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ.
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
✔
EM
EV Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
EV Enriched markers
✔
Canx
EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ.
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
✔
EM
EV Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
EV Enriched markers
✔
Canx
EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ.
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
✔
EM
EV Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
EV Enriched markers
✔
Canx
EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ.
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
5
Experiment ID 217
MISEV standards
✔
EM
EV Biophysical techniques
✔
TSG101|CD81|CD9|CD63
EV Enriched markers
✘ EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23844026    
Organism Homo sapiens
Experiment description Characterization of human thymic exosomes.
Authors Skogberg G, Gudmundsdottir J, van der Post S, Sandstrom K, Bruhn S, Benson M, Mincheva-Nilsson L, Baranov V, Telemo E, Ekwall O.
Journal name PLoS One
Publication year 2013
Sample Thymus
Sample name Normal-Thymus
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for ITGAM
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 AUP1 550
Two-hybrid Homo sapiens
2 CFH 3075
Invitro Homo sapiens
3 PLAUR 5329
Reconstituted Complex Homo sapiens
4 PRTN3 5657
Affinity Capture-Western Homo sapiens
5 JAM3 83700
Invitro Homo sapiens
Invivo Homo sapiens
6 HP 3240
Invivo Homo sapiens
7 ITGB2 3689
Invivo Homo sapiens
8 IRAK1  
Invivo Homo sapiens
9 FGG 2266
Invivo Homo sapiens
Invivo Homo sapiens
10 ICAM1 3383
Invivo Homo sapiens
Invivo Homo sapiens
Invitro Homo sapiens
Invitro Homo sapiens
11 C3 718
Invivo Homo sapiens
Invitro Homo sapiens
12 ICAM4  
Invivo Homo sapiens
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