Gene description for MMGT1
Gene name membrane magnesium transporter 1
Gene symbol MMGT1
Other names/aliases EMC5
TMEM32
Species Homo sapiens
 Database cross references - MMGT1
ExoCarta ExoCarta_93380
Vesiclepedia VP_93380
Entrez Gene 93380
HGNC 28100
UniProt Q8N4V1  
 MMGT1 identified in exosomes derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Hepatocellular carcinoma cells 26054723    
 Gene ontology annotations for MMGT1
Molecular Function
    protein binding GO:0005515 IPI
    cobalt ion transmembrane transporter activity GO:0015087 IEA
    ferrous iron transmembrane transporter activity GO:0015093 IEA
    magnesium ion transmembrane transporter activity GO:0015095 ISS
    inorganic cation transmembrane transporter activity GO:0022890 IBA
    membrane insertase activity GO:0032977 IMP
Biological Process
    cobalt ion transport GO:0006824 IEA
    copper ion transport GO:0006825 IEA
    magnesium ion transport GO:0015693 ISS
    iron ion transmembrane transport GO:0034755 IEA
    protein insertion into ER membrane by stop-transfer membrane-anchor sequence GO:0045050 IDA
    protein insertion into ER membrane by stop-transfer membrane-anchor sequence GO:0045050 IMP
    tail-anchored membrane protein insertion into ER membrane GO:0071816 IDA
    tail-anchored membrane protein insertion into ER membrane GO:0071816 IMP
    magnesium ion transmembrane transport GO:1903830 IEA
Subcellular Localization
    Golgi membrane GO:0000139 IEA
    early endosome GO:0005769 IBA
    early endosome GO:0005769 ISS
    endoplasmic reticulum membrane GO:0005789 IDA
    endoplasmic reticulum membrane GO:0005789 NAS
    Golgi apparatus GO:0005794 IBA
    Golgi apparatus GO:0005794 ISS
    plasma membrane GO:0005886 IBA
    membrane GO:0016020 HDA
    membrane GO:0016020 IDA
    early endosome membrane GO:0031901 IEA
    EMC complex GO:0072546 IBA
    EMC complex GO:0072546 IDA
    EMC complex GO:0072546 IPI
    EMC complex GO:0072546 ISS
 Experiment description of studies that identified MMGT1 in exosomes
1
Experiment ID 489
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
5
Experiment ID 235
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|Alix|HSC70|GAPDH
Enriched markers
✔
HSP90B1
Negative markers
✔
qNano
Particle analysis
Identified molecule mRNA
Identification method RNA Sequencing
PubMed ID 26054723    
Organism Homo sapiens
Experiment description Hepatocellular carcinoma-derived exosomes promote motility of immortalized hepatocyte through transfer of oncogenic proteins and RNAs
Authors "He M, Qin H, Poon TC, Sze SC, Ding X, Co NN, Ngai SM, Chan TF, Wong N"
Journal name Carcinogenesis
Publication year 2015
Sample Hepatocellular carcinoma cells
Sample name HKCI-8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
RNA
Methods used in the study Western blotting
Mass spectrometry
RT-PCR
RNA Sequencing
 Protein-protein interactions for MMGT1
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 SEC22B 9554
Two-hybrid Homo sapiens
2 CCAR1 55749
Affinity Capture-MS Homo sapiens
3 EMC4 51234
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
4 TAS2R19  
Two-hybrid Homo sapiens
5 HSD17B11 51170
Proximity Label-MS Homo sapiens
6 Tmed2 56334
Affinity Capture-MS Mus musculus
7 TMBIM6 7009
Two-hybrid Homo sapiens
8 TMEM60  
Two-hybrid Homo sapiens
9 DNAJC8 22826
Affinity Capture-MS Homo sapiens
10 YIF1A 10897
Two-hybrid Homo sapiens
11 VAPA 9218
Affinity Capture-MS Homo sapiens
12 METTL7A 25840
Proximity Label-MS Homo sapiens
13 PPIL4  
Affinity Capture-MS Homo sapiens
14 THBD 7056
Two-hybrid Homo sapiens
15 TM9SF2 9375
Co-fractionation Homo sapiens
16 LAP3 51056
Affinity Capture-MS Homo sapiens
17 SF3B1 23451
Affinity Capture-MS Homo sapiens
18 MFSD5  
Two-hybrid Homo sapiens
19 CORO7 79585
Affinity Capture-MS Homo sapiens
20 GJD3  
Proximity Label-MS Homo sapiens
21 DNAJC25 548645
Proximity Label-MS Homo sapiens
22 GOSR2 9570
Two-hybrid Homo sapiens
23 LNPEP 4012
Two-hybrid Homo sapiens
24 MED24 9862
Affinity Capture-MS Homo sapiens
25 PTPN1 5770
Proximity Label-MS Homo sapiens
26 Ktn1  
Affinity Capture-MS Mus musculus
27 MFSD3  
Two-hybrid Homo sapiens
28 APP 351
Reconstituted Complex Homo sapiens
29 BRWD1  
Affinity Capture-MS Homo sapiens
30 AGR3 155465
Two-hybrid Homo sapiens
31 RUVBL1 8607
Cross-Linking-MS (XL-MS) Homo sapiens
32 B3GAT1  
Proximity Label-MS Homo sapiens
33 PGAM5 192111
Co-fractionation Homo sapiens
34 STX1A 6804
Two-hybrid Homo sapiens
35 INSRR 3645
Affinity Capture-MS Homo sapiens
36 PRKCDBP 112464
Affinity Capture-MS Homo sapiens
37 NDUFB8 4714
Co-fractionation Homo sapiens
38 SLC35B4  
Two-hybrid Homo sapiens
39 Emc3  
Affinity Capture-MS Mus musculus
40 GRM2 2912
Two-hybrid Homo sapiens
41 C4orf3  
Two-hybrid Homo sapiens
42 RAB3B 5865
Proximity Label-MS Homo sapiens
43 IMPDH2 3615
Affinity Capture-MS Homo sapiens
44 STX8 9482
Two-hybrid Homo sapiens
45 RCBTB2  
Affinity Capture-MS Homo sapiens
46 GPRC5B 51704
Affinity Capture-MS Homo sapiens
47 PNPLA6 10908
Affinity Capture-MS Homo sapiens
48 SSR1 6745
Proximity Label-MS Homo sapiens
49 RIOK3 8780
Affinity Capture-MS Homo sapiens
50 PIGH  
Affinity Capture-MS Homo sapiens
51 MKI67  
Affinity Capture-MS Homo sapiens
52 NDUFA3  
Two-hybrid Homo sapiens
Affinity Capture-MS Homo sapiens
53 MS4A13  
Two-hybrid Homo sapiens
54 C20orf24  
Affinity Capture-MS Homo sapiens
55 STX6 10228
Two-hybrid Homo sapiens
56 VDAC1 7416
Affinity Capture-MS Homo sapiens
57 SLC25A46 91137
Proximity Label-MS Homo sapiens
58 PHB 5245
Affinity Capture-MS Homo sapiens
59 ABCC2 1244
Co-fractionation Homo sapiens
60 NINJ2  
Two-hybrid Homo sapiens
61 NDUFS1 4719
Affinity Capture-MS Homo sapiens
62 ITGAM 3684
Two-hybrid Homo sapiens
63 STX12 23673
Two-hybrid Homo sapiens
64 MARCKS 4082
Proximity Label-MS Homo sapiens
65 TMEM254  
Two-hybrid Homo sapiens
66 EMC1 23065
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
67 EMC9  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
68 INSIG2  
Two-hybrid Homo sapiens
69 STOML2 30968
Affinity Capture-MS Homo sapiens
70 PXMP2  
Proximity Label-MS Homo sapiens
71 ZMAT3  
Affinity Capture-MS Homo sapiens
72 NDUFV2 4729
Affinity Capture-MS Homo sapiens
73 PANX1 24145
Proximity Label-MS Homo sapiens
74 MPV17 4358
Co-fractionation Homo sapiens
75 EMC10 284361
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
76 GPR25  
Two-hybrid Homo sapiens
77 TPR 7175
Affinity Capture-MS Homo sapiens
78 EMD 2010
Two-hybrid Homo sapiens
Proximity Label-MS Homo sapiens
79 NDUFB7 4713
Affinity Capture-MS Homo sapiens
80 RBM6 10180
Affinity Capture-MS Homo sapiens
81 TMED9 54732
Affinity Capture-MS Homo sapiens
82 CHCHD3 54927
Affinity Capture-MS Homo sapiens
83 C5 727
Two-hybrid Homo sapiens
84 HDGFRP2 84717
Affinity Capture-MS Homo sapiens
85 TMEM43 79188
Affinity Capture-MS Homo sapiens
86 DEK 7913
Affinity Capture-MS Homo sapiens
87 PARD3 56288
Affinity Capture-MS Homo sapiens
88 C16orf58 64755
Two-hybrid Homo sapiens
89 UBQLN2 29978
Affinity Capture-MS Homo sapiens
90 HLA-DRB5 3127
Affinity Capture-MS Homo sapiens
91 KIAA1143  
Affinity Capture-MS Homo sapiens
92 OCIAD2 132299
Co-fractionation Homo sapiens
93 UQCRC1 7384
Affinity Capture-MS Homo sapiens
94 RPN1 6184
Proximity Label-MS Homo sapiens
95 PTRF 284119
Affinity Capture-MS Homo sapiens
96 TMEM63A 9725
Affinity Capture-MS Homo sapiens
97 GCSH 2653
Affinity Capture-MS Homo sapiens
98 POLB 5423
Affinity Capture-MS Homo sapiens
99 TMEM33 55161
Affinity Capture-MS Homo sapiens
100 FLOT2 2319
Co-fractionation Homo sapiens
Affinity Capture-MS Homo sapiens
101 ELOVL5 60481
Proximity Label-MS Homo sapiens
102 MFF 56947
Two-hybrid Homo sapiens
103 TTC31  
Affinity Capture-MS Homo sapiens
104 SEC61B 10952
Proximity Label-MS Homo sapiens
105 GLTSCR2  
Affinity Capture-MS Homo sapiens
106 RMDN3 55177
Proximity Label-MS Homo sapiens
107 COX4I1 1327
Affinity Capture-MS Homo sapiens
108 REEP5 7905
Proximity Label-MS Homo sapiens
109 ANLN 54443
Affinity Capture-MS Homo sapiens
110 TMEM14C 51522
Two-hybrid Homo sapiens
111 CT45A1  
Affinity Capture-MS Homo sapiens
112 SEC63 11231
Proximity Label-MS Homo sapiens
113 Rab5c 19345
Affinity Capture-MS Mus musculus
114 RBM5  
Affinity Capture-MS Homo sapiens
115 C4orf32  
Affinity Capture-MS Homo sapiens
116 HADHB 3032
Co-fractionation Homo sapiens
117 EMC3 55831
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
118 RPN2 6185
Proximity Label-MS Homo sapiens
119 CANX 821
Affinity Capture-MS Homo sapiens
120 EMC8 10328
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
121 LRRC59 55379
Proximity Label-MS Homo sapiens
122 EGLN3  
Affinity Capture-MS Homo sapiens
123 RC3H2  
Affinity Capture-MS Homo sapiens
124 MTX2 10651
Co-fractionation Homo sapiens
125 TMED8 283578
Two-hybrid Homo sapiens
126 Emc6  
Affinity Capture-MS Mus musculus
127 C19orf43  
Affinity Capture-MS Homo sapiens
128 GOLT1B 51026
Affinity Capture-MS Homo sapiens
129 Rmdn3  
Affinity Capture-MS Mus musculus
130 BCAP31 10134
Proximity Label-MS Homo sapiens
131 HSD3B7 80270
Proximity Label-MS Homo sapiens
132 STIM1 6786
Proximity Label-MS Homo sapiens
133 FXYD6  
Two-hybrid Homo sapiens
134 TMEM42  
Two-hybrid Homo sapiens
135 FKBP8 23770
Proximity Label-MS Homo sapiens
136 CORO1C 23603
Co-fractionation Homo sapiens
137 LPAR1 1902
Affinity Capture-MS Homo sapiens
138 TTC9C 283237
Affinity Capture-MS Homo sapiens
139 MRPL1  
Co-fractionation Homo sapiens
140 PHB2 11331
Affinity Capture-MS Homo sapiens
141 NSRP1  
Affinity Capture-MS Homo sapiens
142 PEX16 9409
Two-hybrid Homo sapiens
143 MFAP1  
Affinity Capture-MS Homo sapiens
144 TMEM229B  
Two-hybrid Homo sapiens
145 ATP2A1 487
Proximity Label-MS Homo sapiens
146 EMC2 9694
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
147 RAB9A 9367
Proximity Label-MS Homo sapiens
148 BRD4 23476
Affinity Capture-MS Homo sapiens
149 NCLN 56926
Affinity Capture-MS Homo sapiens
150 NDUFA7 4701
Affinity Capture-MS Homo sapiens
151 CCDC124 115098
Affinity Capture-MS Homo sapiens
152 DHFRL1  
Proximity Label-MS Homo sapiens
153 RPS11 6205
Affinity Capture-MS Homo sapiens
154 KTN1 3895
Affinity Capture-MS Homo sapiens
155 GALNT2 2590
Affinity Capture-MS Homo sapiens
156 PPAPDC2  
Two-hybrid Homo sapiens
157 DLAT 1737
Co-fractionation Homo sapiens
158 LMNA 4000
Proximity Label-MS Homo sapiens
Proximity Label-MS Homo sapiens
159 ADAR 103
Affinity Capture-MS Homo sapiens
160 CHCHD6 84303
Affinity Capture-MS Homo sapiens
161 FLOT1 10211
Affinity Capture-MS Homo sapiens
162 RBM17 84991
Affinity Capture-MS Homo sapiens
163 KIAA1522 57648
Affinity Capture-MS Homo sapiens
164 ACBD5 91452
Affinity Capture-MS Homo sapiens
165 ORMDL2 29095
Two-hybrid Homo sapiens
166 IMMT 10989
Affinity Capture-MS Homo sapiens
167 EMC6 83460
Two-hybrid Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
168 LBR 3930
Affinity Capture-MS Homo sapiens
169 DHX15 1665
Co-fractionation Homo sapiens
170 TMEM55B 90809
Affinity Capture-MS Homo sapiens
171 TRAF3IP2  
Affinity Capture-MS Homo sapiens
172 Emc1 230866
Affinity Capture-MS Mus musculus
173 KIF1C 10749
Affinity Capture-MS Homo sapiens
174 LIG3 3980
Affinity Capture-MS Homo sapiens
175 HLA-DRB1 3123
Affinity Capture-MS Homo sapiens
176 MCAT 27349
Affinity Capture-MS Homo sapiens
177 C5orf24 134553
Affinity Capture-MS Homo sapiens
178 EMC7 56851
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
179 EMR2  
Two-hybrid Homo sapiens
180 SAP18 10284
Affinity Capture-MS Homo sapiens
181 HNRNPA2B1 3181
Affinity Capture-MS Homo sapiens
182 PDHA1 5160
Affinity Capture-MS Homo sapiens
183 IMPDH1 3614
Affinity Capture-MS Homo sapiens
184 TMEM86B  
Two-hybrid Homo sapiens
185 NDUFA12 55967
Co-fractionation Homo sapiens
Affinity Capture-MS Homo sapiens
186 ELAVL1 1994
Affinity Capture-RNA Homo sapiens
187 NADSYN1 55191
Affinity Capture-MS Homo sapiens
188 DMAP1 55929
Affinity Capture-MS Homo sapiens
189 CAV1 857
Affinity Capture-MS Homo sapiens
190 ERGIC1 57222
Proximity Label-MS Homo sapiens
191 ATP2A2 488
Affinity Capture-MS Homo sapiens
192 SLC38A7 55238
Two-hybrid Homo sapiens
193 VDAC2 7417
Affinity Capture-MS Homo sapiens
194 GOLIM4 27333
Affinity Capture-MS Homo sapiens
195 ERGIC2 51290
Proximity Label-MS Homo sapiens
196 OSBPL3 26031
Affinity Capture-MS Homo sapiens
197 FIS1 51024
Two-hybrid Homo sapiens
198 NDUFA2 4695
Affinity Capture-MS Homo sapiens
199 SEC62 7095
Proximity Label-MS Homo sapiens
200 STX3 6809
Two-hybrid Homo sapiens
201 TMEM109 79073
Affinity Capture-MS Homo sapiens
202 CYB5R3 1727
Co-fractionation Homo sapiens
203 SCAF8 22828
Affinity Capture-MS Homo sapiens
204 DNAJC1 64215
Proximity Label-MS Homo sapiens
205 RAB5C 5878
Proximity Label-MS Homo sapiens
206 GOLM1 51280
Affinity Capture-MS Homo sapiens
207 CCDC47 57003
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
208 CKAP4 10970
Proximity Label-MS Homo sapiens
Affinity Capture-MS Homo sapiens
209 PCDHB3  
Affinity Capture-MS Homo sapiens
210 ANKRD46 157567
Two-hybrid Homo sapiens
211 NEMF 9147
Affinity Capture-MS Homo sapiens
212 C8A 731
Two-hybrid Homo sapiens
213 NDUFS6  
Affinity Capture-MS Homo sapiens
214 PCNP 57092
Affinity Capture-MS Homo sapiens