Gene description for ORMDL2
Gene name ORMDL sphingolipid biosynthesis regulator 2
Gene symbol ORMDL2
Other names/aliases HSPC160
MST095
MSTP095
adoplin-2
Species Homo sapiens
 Database cross references - ORMDL2
ExoCarta ExoCarta_29095
Vesiclepedia VP_29095
Entrez Gene 29095
HGNC 16037
MIM 610074
UniProt Q53FV1  
 ORMDL2 identified in exosomes derived from the following tissue/cell type
Platelets 25332113    
Platelets 25332113    
Platelets 25332113    
Retinal pigment epithelial cells 35333565    
Retinal pigment epithelial cells 35333565    
 Gene ontology annotations for ORMDL2
Molecular Function
    protein binding GO:0005515 IPI
Biological Process
    ceramide metabolic process GO:0006672 IBA
    ceramide metabolic process GO:0006672 IMP
    sphingolipid biosynthetic process GO:0030148 IBA
    intracellular sphingolipid homeostasis GO:0090156 IBA
    negative regulation of ceramide biosynthetic process GO:1900060 IMP
Subcellular Localization
    endoplasmic reticulum GO:0005783 IDA
    endoplasmic reticulum membrane GO:0005789 IEA
    serine palmitoyltransferase complex GO:0017059 IBA
 Experiment description of studies that identified ORMDL2 in exosomes
1
Experiment ID 231
MISEV standards
✘ Biophysical techniques
✔
Alix|CD63|CD9
Enriched markers
✘ Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25332113    
Organism Homo sapiens
Experiment description Lipidomic and proteomic characterization of platelet extracellular vesicle subfractions from senescent platelets
Authors "Pienimaeki-Roemer A, Kuhlmann K, Bottcher A, Konovalova T, Black A, Orso E, Liebisch G, Ahrens M, Eisenacher M, Meyer HE, Schmitz G."
Journal name Transfusion
Publication year 2015
Sample Platelets
Sample name PL-Exs - Rep 1
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Optiprep density gradient
Flotation density 1.12-1.15 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Western blotting
Mass spectrometry
2
Experiment ID 232
MISEV standards
✘ Biophysical techniques
✘ Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25332113    
Organism Homo sapiens
Experiment description Lipidomic and proteomic characterization of platelet extracellular vesicle subfractions from senescent platelets
Authors "Pienimaeki-Roemer A, Kuhlmann K, Bottcher A, Konovalova T, Black A, Orso E, Liebisch G, Ahrens M, Eisenacher M, Meyer HE, Schmitz G."
Journal name Transfusion
Publication year 2015
Sample Platelets
Sample name PL-Exs - Rep 2
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Optiprep density gradient
Flotation density 1.12-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
3
Experiment ID 233
MISEV standards
✘ Biophysical techniques
✘ Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25332113    
Organism Homo sapiens
Experiment description Lipidomic and proteomic characterization of platelet extracellular vesicle subfractions from senescent platelets
Authors "Pienimaeki-Roemer A, Kuhlmann K, Bottcher A, Konovalova T, Black A, Orso E, Liebisch G, Ahrens M, Eisenacher M, Meyer HE, Schmitz G."
Journal name Transfusion
Publication year 2015
Sample Platelets
Sample name PL-Exs - Rep 3
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Optiprep density gradient
Flotation density 1.12-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
4
Experiment ID 834
MISEV standards
✔
EM
Biophysical techniques
✔
LAMP2|CD63|FLOT1|ITGA2B|ICAM1|CD9|CD151|TFRC|RAB5A|GAPDH|AQP1|TSG101
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35333565    
Organism Homo sapiens
Experiment description LAMP2A regulates the loading of proteins into exosomes
Authors "Ferreira JV, da Rosa Soares A, Ramalho J, Máximo Carvalho C, Cardoso MH, Pintado P, Carvalho AS, Beck HC, Matthiesen R, Zuzarte M, Girão H, van Niel G, Pereira P"
Journal name Sci Adv
Publication year 2022
Sample Retinal pigment epithelial cells
Sample name ARPE-19
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
5
Experiment ID 835
MISEV standards
✔
EM
Biophysical techniques
✔
CD63|FLOT1|ITGA2B|ICAM1|CD9|CD151|TFRC|RAB5A|GAPDH|AQP1|TSG101
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35333565    
Organism Homo sapiens
Experiment description LAMP2A regulates the loading of proteins into exosomes
Authors "Ferreira JV, da Rosa Soares A, Ramalho J, Máximo Carvalho C, Cardoso MH, Pintado P, Carvalho AS, Beck HC, Matthiesen R, Zuzarte M, Girão H, van Niel G, Pereira P"
Journal name Sci Adv
Publication year 2022
Sample Retinal pigment epithelial cells
Sample name ARPE-19
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for ORMDL2
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 SLC2A12 154091
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
2 GJA8  
Two-hybrid Homo sapiens
3 RPN2 6185
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
4 ATP5J 522
Affinity Capture-MS Homo sapiens
5 SLC5A5 6528
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
6 SLC39A4 55630
Affinity Capture-MS Homo sapiens
7 TMEM120A 83862
Two-hybrid Homo sapiens
8 PTH2R  
Affinity Capture-MS Homo sapiens
9 SLC26A6 65010
Affinity Capture-MS Homo sapiens
10 FCER1G 2207
Two-hybrid Homo sapiens
11 CRELD1 78987
Affinity Capture-MS Homo sapiens
12 BSCL2  
Affinity Capture-MS Homo sapiens
13 SPTLC1 10558
Affinity Capture-MS Homo sapiens
14 NPY2R  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
15 CPQ 10404
Affinity Capture-MS Homo sapiens
16 SLC5A6 8884
Affinity Capture-MS Homo sapiens
17 EBP  
Two-hybrid Homo sapiens
18 CD79A  
Two-hybrid Homo sapiens
19 SLCO1B1  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
20 OPRL1 4987
Affinity Capture-MS Homo sapiens
21 SLC22A9  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
22 MMGT1 93380
Two-hybrid Homo sapiens
23 TMEM86B  
Two-hybrid Homo sapiens
24 SLC18A2  
Affinity Capture-MS Homo sapiens
25 FAM134C 162427
Two-hybrid Homo sapiens
26 SLC10A1  
Two-hybrid Homo sapiens
27 GABBR1  
Affinity Capture-MS Homo sapiens
28 KCNJ6  
Two-hybrid Homo sapiens
29 TMX2 51075
Two-hybrid Homo sapiens
30 TMEM31  
Two-hybrid Homo sapiens
31 SLC22A4 6583
Affinity Capture-MS Homo sapiens
32 SCN3B  
Two-hybrid Homo sapiens
33 ELOVL4  
Two-hybrid Homo sapiens
34 GPR183  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
35 ZDHHC15 158866
Two-hybrid Homo sapiens
36 TTYH1  
Affinity Capture-MS Homo sapiens
37 CREB3L1  
Two-hybrid Homo sapiens
38 FAM210B  
Two-hybrid Homo sapiens
View the network image/svg+xml
 Pathways in which ORMDL2 is involved
PathwayEvidenceSource
Metabolism TAS Reactome
Metabolism of lipids TAS Reactome
Sphingolipid de novo biosynthesis TAS Reactome
Sphingolipid metabolism TAS Reactome





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