Gene description for TMEM120A
Gene name transmembrane protein 120A
Gene symbol TMEM120A
Other names/aliases NET29
TMPIT
Species Homo sapiens
 Database cross references - TMEM120A
ExoCarta ExoCarta_83862
Vesiclepedia VP_83862
Entrez Gene 83862
HGNC 21697
UniProt Q9BXJ8  
 TMEM120A identified in exosomes derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Retinal pigment epithelial cells 35333565    
Retinal pigment epithelial cells 35333565    
 Gene ontology annotations for TMEM120A
Molecular Function
    monoatomic ion channel activity GO:0005216 IDA
    monoatomic ion channel activity GO:0005216 IMP
    monoatomic ion channel activity GO:0005216 ISS
    protein binding GO:0005515 IPI
    coenzyme A binding GO:0120225 IDA
Biological Process
    monoatomic ion transmembrane transport GO:0034220 ISS
    fat cell differentiation GO:0045444 IBA
    fat cell differentiation GO:0045444 IMP
    detection of mechanical stimulus involved in sensory perception of pain GO:0050966 ISS
    protein homooligomerization GO:0051260 IDA
    protein heterooligomerization GO:0051291 IDA
    antiviral innate immune response GO:0140374 IMP
Subcellular Localization
    nuclear inner membrane GO:0005637 IEA
    endoplasmic reticulum GO:0005783 IDA
    plasma membrane GO:0005886 IDA
    plasma membrane GO:0005886 ISS
    membrane GO:0016020 IBA
 Experiment description of studies that identified TMEM120A in exosomes
1
Experiment ID 489
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
5
Experiment ID 834
MISEV standards
✔
EM
Biophysical techniques
✔
LAMP2|CD63|FLOT1|ITGA2B|ICAM1|CD9|CD151|TFRC|RAB5A|GAPDH|AQP1|TSG101
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35333565    
Organism Homo sapiens
Experiment description LAMP2A regulates the loading of proteins into exosomes
Authors "Ferreira JV, da Rosa Soares A, Ramalho J, Máximo Carvalho C, Cardoso MH, Pintado P, Carvalho AS, Beck HC, Matthiesen R, Zuzarte M, Girão H, van Niel G, Pereira P"
Journal name Sci Adv
Publication year 2022
Sample Retinal pigment epithelial cells
Sample name ARPE-19
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
6
Experiment ID 834
MISEV standards
✔
EM
Biophysical techniques
✔
LAMP2|CD63|FLOT1|ITGA2B|ICAM1|CD9|CD151|TFRC|RAB5A|GAPDH|AQP1|TSG101
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35333565    
Organism Homo sapiens
Experiment description LAMP2A regulates the loading of proteins into exosomes
Authors "Ferreira JV, da Rosa Soares A, Ramalho J, Máximo Carvalho C, Cardoso MH, Pintado P, Carvalho AS, Beck HC, Matthiesen R, Zuzarte M, Girão H, van Niel G, Pereira P"
Journal name Sci Adv
Publication year 2022
Sample Retinal pigment epithelial cells
Sample name ARPE-19
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
7
Experiment ID 835
MISEV standards
✔
EM
Biophysical techniques
✔
CD63|FLOT1|ITGA2B|ICAM1|CD9|CD151|TFRC|RAB5A|GAPDH|AQP1|TSG101
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35333565    
Organism Homo sapiens
Experiment description LAMP2A regulates the loading of proteins into exosomes
Authors "Ferreira JV, da Rosa Soares A, Ramalho J, Máximo Carvalho C, Cardoso MH, Pintado P, Carvalho AS, Beck HC, Matthiesen R, Zuzarte M, Girão H, van Niel G, Pereira P"
Journal name Sci Adv
Publication year 2022
Sample Retinal pigment epithelial cells
Sample name ARPE-19
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
8
Experiment ID 835
MISEV standards
✔
EM
Biophysical techniques
✔
CD63|FLOT1|ITGA2B|ICAM1|CD9|CD151|TFRC|RAB5A|GAPDH|AQP1|TSG101
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35333565    
Organism Homo sapiens
Experiment description LAMP2A regulates the loading of proteins into exosomes
Authors "Ferreira JV, da Rosa Soares A, Ramalho J, Máximo Carvalho C, Cardoso MH, Pintado P, Carvalho AS, Beck HC, Matthiesen R, Zuzarte M, Girão H, van Niel G, Pereira P"
Journal name Sci Adv
Publication year 2022
Sample Retinal pigment epithelial cells
Sample name ARPE-19
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for TMEM120A
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 TMEM35  
Two-hybrid Homo sapiens
2 METTL9 51108
Affinity Capture-MS Homo sapiens
3 GALNT10 55568
Affinity Capture-MS Homo sapiens
4 CD1A 909
Affinity Capture-MS Homo sapiens
5 KCNMB3  
Affinity Capture-MS Homo sapiens
6 RAMP2  
Affinity Capture-MS Homo sapiens
7 CXCR4 7852
Affinity Capture-MS Homo sapiens
8 TTYH1  
Affinity Capture-MS Homo sapiens
9 PAXIP1  
Two-hybrid Homo sapiens
10 ATP5J 522
Affinity Capture-MS Homo sapiens
11 C16orf58 64755
Affinity Capture-MS Homo sapiens
12 SLC39A4 55630
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
13 LDLRAD1  
Affinity Capture-MS Homo sapiens
14 HCST  
Affinity Capture-MS Homo sapiens
15 LEPROT 54741
Two-hybrid Homo sapiens
16 NOMO1 23420
Affinity Capture-MS Homo sapiens
17 LRRC25  
Two-hybrid Homo sapiens
18 LRRC55  
Affinity Capture-MS Homo sapiens
19 CLEC2D  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
20 Bmpr1a  
Affinity Capture-MS Mus musculus
21 TMEM120B  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
22 CRELD1 78987
Affinity Capture-MS Homo sapiens
23 TMEM14B  
Two-hybrid Homo sapiens
24 Atp2a2 11938
Affinity Capture-MS Mus musculus
25 CA3 761
Affinity Capture-MS Homo sapiens
26 TMEM86B  
Two-hybrid Homo sapiens
27 UBC 7316
Reconstituted Complex Homo sapiens
28 TSPAN18 90139
Two-hybrid Homo sapiens
29 HSD17B13  
Two-hybrid Homo sapiens
30 AGPAT3 56894
Affinity Capture-MS Homo sapiens
31 TNFSF18 8995
Affinity Capture-MS Homo sapiens
32 SERINC1 57515
Affinity Capture-MS Homo sapiens
33 PPP1CC 5501
Two-hybrid Homo sapiens
34 FAM209A 200232
Two-hybrid Homo sapiens
35 KDSR 2531
Affinity Capture-MS Homo sapiens
36 SYPL2  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
37 STX1A 6804
Two-hybrid Homo sapiens
38 TPRG1  
Two-hybrid Homo sapiens
39 CTLA4  
Affinity Capture-MS Homo sapiens
40 PRKCA 5578
Affinity Capture-MS Homo sapiens
41 POMK  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
42 NCLN 56926
Affinity Capture-MS Homo sapiens
43 VKORC1 79001
Two-hybrid Homo sapiens
44 ARL15 54622
Affinity Capture-MS Homo sapiens
45 ATP5C1 509
Affinity Capture-MS Homo sapiens
46 B4GALT3 8703
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
47 GPRC5B 51704
Two-hybrid Homo sapiens
48 UPK1A 11045
Affinity Capture-MS Homo sapiens
49 SLC22A4 6583
Affinity Capture-MS Homo sapiens
50 JTB 10899
Affinity Capture-MS Homo sapiens
51 SCN3B  
Two-hybrid Homo sapiens
52 STX2 2054
Two-hybrid Homo sapiens
53 ATP5B 506
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
54 PTAFR  
Two-hybrid Homo sapiens
55 TMX2 51075
Two-hybrid Homo sapiens
56 YIPF3 25844
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
57 ORMDL2 29095
Two-hybrid Homo sapiens
58 SNAP29 9342
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
View the network image/svg+xml
 Pathways in which TMEM120A is involved
No pathways found





Perform bioinformatics analysis of your extracellular vesicle data set using FunRich, a open access standalone tool. NEW UPDATED VERSION OF FunRich available for download (12/09/2016) from here