Gene description for NCLN
Gene name nicalin
Gene symbol NCLN
Other names/aliases NET59
Species Homo sapiens
 Database cross references - NCLN
ExoCarta ExoCarta_56926
Vesiclepedia VP_56926
Entrez Gene 56926
HGNC 26923
MIM 609156
UniProt Q969V3  
 NCLN identified in exosomes derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Retinal pigment epithelial cells 35333565    
Retinal pigment epithelial cells 35333565    
 Gene ontology annotations for NCLN
Molecular Function
    protein binding GO:0005515 IPI
    ribosome binding GO:0043022 IDA
Biological Process
    determination of left/right asymmetry in lateral mesoderm GO:0003140 ISS
    regulation of signal transduction GO:0009966 IBA
    regulation of protein-containing complex assembly GO:0043254 IDA
    protein stabilization GO:0050821 IMP
    regulation of protein complex stability GO:0061635 IDA
    multi-pass transmembrane protein insertion into ER membrane GO:0160063 IDA
    negative regulation of nodal signaling pathway GO:1900108 ISS
Subcellular Localization
    endoplasmic reticulum membrane GO:0005789 IBA
    endoplasmic reticulum membrane GO:0005789 IDA
    membrane GO:0016020 HDA
    protein-containing complex GO:0032991 IDA
    multi-pass translocon complex GO:0160064 IDA
 Experiment description of studies that identified NCLN in exosomes
1
Experiment ID 489
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
5
Experiment ID 834
MISEV standards
✔
EM
Biophysical techniques
✔
LAMP2|CD63|FLOT1|ITGA2B|ICAM1|CD9|CD151|TFRC|RAB5A|GAPDH|AQP1|TSG101
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35333565    
Organism Homo sapiens
Experiment description LAMP2A regulates the loading of proteins into exosomes
Authors "Ferreira JV, da Rosa Soares A, Ramalho J, Máximo Carvalho C, Cardoso MH, Pintado P, Carvalho AS, Beck HC, Matthiesen R, Zuzarte M, Girão H, van Niel G, Pereira P"
Journal name Sci Adv
Publication year 2022
Sample Retinal pigment epithelial cells
Sample name ARPE-19
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
6
Experiment ID 835
MISEV standards
✔
EM
Biophysical techniques
✔
CD63|FLOT1|ITGA2B|ICAM1|CD9|CD151|TFRC|RAB5A|GAPDH|AQP1|TSG101
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35333565    
Organism Homo sapiens
Experiment description LAMP2A regulates the loading of proteins into exosomes
Authors "Ferreira JV, da Rosa Soares A, Ramalho J, Máximo Carvalho C, Cardoso MH, Pintado P, Carvalho AS, Beck HC, Matthiesen R, Zuzarte M, Girão H, van Niel G, Pereira P"
Journal name Sci Adv
Publication year 2022
Sample Retinal pigment epithelial cells
Sample name ARPE-19
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for NCLN
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 UBE2H 7328
Affinity Capture-MS Homo sapiens
2 EMC2 9694
Affinity Capture-MS Homo sapiens
3 COIL  
Proximity Label-MS Homo sapiens
4 Psmb5 19173
Affinity Capture-MS Mus musculus
5 VAPA 9218
Affinity Capture-MS Homo sapiens
6 ARL15 54622
Affinity Capture-MS Homo sapiens
7 TMEM147 10430
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
8 NOP56 10528
Proximity Label-MS Homo sapiens
9 SLC2A1 6513
Affinity Capture-MS Homo sapiens
10 PLD4  
Affinity Capture-MS Homo sapiens
11 KRTAP5-9  
Two-hybrid Homo sapiens
12 CKB 1152
Affinity Capture-MS Homo sapiens
13 KIAA1429 25962
Affinity Capture-MS Homo sapiens
14 PARK2  
Affinity Capture-MS Homo sapiens
15 TBL2 26608
Affinity Capture-MS Homo sapiens
16 Kcnk1  
Affinity Capture-MS Mus musculus
17 MCM2 4171
Affinity Capture-MS Homo sapiens
18 CYP19A1  
Affinity Capture-MS Homo sapiens
19 FBXW7  
Affinity Capture-MS Homo sapiens
20 GLMP 112770
Affinity Capture-MS Homo sapiens
21 LRRC8C 84230
Affinity Capture-MS Homo sapiens
22 GPR55  
Affinity Capture-MS Homo sapiens
23 ATP2A2 488
Affinity Capture-MS Homo sapiens
24 PIGH  
Affinity Capture-MS Homo sapiens
25 PGRMC2 10424
Affinity Capture-MS Homo sapiens
26 DHCR7 1717
Affinity Capture-MS Homo sapiens
27 EXD2  
Proximity Label-MS Homo sapiens
28 CCDC47 57003
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
29 SEC11C 90701
Affinity Capture-MS Homo sapiens
30 F2RL1  
Affinity Capture-MS Homo sapiens
31 TMEM106A  
Affinity Capture-MS Homo sapiens
32 EBAG9 9166
Proximity Label-MS Homo sapiens
33 FAM20C 56975
Affinity Capture-MS Homo sapiens
34 RPL19 6143
Affinity Capture-MS Homo sapiens
35 RBM8A 9939
Affinity Capture-MS Homo sapiens
36 EMC1 23065
Affinity Capture-MS Homo sapiens
37 TNF  
Affinity Capture-MS Homo sapiens
38 APOO 79135
Proximity Label-MS Homo sapiens
39 TMEM120A 83862
Affinity Capture-MS Homo sapiens
40 KCNK1  
Affinity Capture-MS Homo sapiens
41 NOMO1 23420
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
42 HIST1H4A 8359
Affinity Capture-MS Homo sapiens
43 CTDP1 9150
Affinity Capture-MS Homo sapiens
44 RB1CC1 9821
Affinity Capture-MS Homo sapiens
45 RPL4 6124
Affinity Capture-MS Homo sapiens
46 DRG1 4733
Affinity Capture-MS Homo sapiens
47 MAGT1 84061
Affinity Capture-MS Homo sapiens
48 XRCC3  
Affinity Capture-MS Homo sapiens
49 NTRK1 4914
Affinity Capture-MS Homo sapiens
50 PIGT 51604
Affinity Capture-MS Homo sapiens
51 GPR182  
Affinity Capture-MS Homo sapiens
52 C20orf24  
Affinity Capture-MS Homo sapiens
53 NOMO3 408050
Affinity Capture-MS Homo sapiens
54 HUWE1 10075
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
55 RPN1 6184
Affinity Capture-MS Homo sapiens
56 DNAJC30  
Proximity Label-MS Homo sapiens
57 TMEM33 55161
Affinity Capture-MS Homo sapiens
58 SEC61B 10952
Proximity Label-MS Homo sapiens
59 SLC33A1 9197
Affinity Capture-MS Homo sapiens
60 COX4I1 1327
Proximity Label-MS Homo sapiens
61 SSR4 6748
Co-fractionation Homo sapiens
62 SEC63 11231
Affinity Capture-MS Homo sapiens
63 NOMO2 283820
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
64 RPN2 6185
Co-fractionation Homo sapiens
Affinity Capture-MS Homo sapiens
65 CANX 821
Co-fractionation Homo sapiens
66 SELT 51714
Affinity Capture-MS Homo sapiens
67 LRRC59 55379
Proximity Label-MS Homo sapiens
68 LAMP1 3916
Proximity Label-MS Homo sapiens
69 CUL3 8452
Affinity Capture-MS Homo sapiens
70 NR3C1 2908
Proximity Label-MS Homo sapiens
71 CBLN4  
Affinity Capture-MS Homo sapiens
72 EGFR 1956
Affinity Capture-MS Homo sapiens
73 ARF6 382
Proximity Label-MS Homo sapiens
74 FBXO6 26270
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
75 EPRS 2058
Co-fractionation Homo sapiens
76 SERINC1 57515
Affinity Capture-MS Homo sapiens
77 SPCS3 60559
Affinity Capture-MS Homo sapiens
78 RPA3 6119
Proximity Label-MS Homo sapiens
79 FBXO2 26232
Affinity Capture-MS Homo sapiens
80 TMCO1 54499
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
81 Cct8 12469
Affinity Capture-MS Mus musculus
82 DDRGK1 65992
Affinity Capture-MS Homo sapiens
83 RHOB 388
Proximity Label-MS Homo sapiens
84 METTL9 51108
Affinity Capture-MS Homo sapiens
85 PEX3 8504
Proximity Label-MS Homo sapiens
86 AKAP1 8165
Proximity Label-MS Homo sapiens
87 MLEC 9761
Affinity Capture-MS Homo sapiens
88 CCDC8  
Affinity Capture-MS Homo sapiens
89 TMX2 51075
Affinity Capture-MS Homo sapiens
90 EMC6 83460
Affinity Capture-MS Homo sapiens
91 LBR 3930
Affinity Capture-MS Homo sapiens
92 CLPP 8192
Proximity Label-MS Homo sapiens
93 PLEKHA4 57664
Affinity Capture-MS Homo sapiens
94 RAB3D 9545
Affinity Capture-MS Homo sapiens
95 ILF3 3609
Co-fractionation Homo sapiens
96 PGRMC1 10857
Affinity Capture-MS Homo sapiens
97 TMEM214 54867
Affinity Capture-MS Homo sapiens
98 PDIA4 9601
Proximity Label-MS Homo sapiens
99 CALU 813
Proximity Label-MS Homo sapiens
100 SEC11A 23478
Affinity Capture-MS Homo sapiens
101 EMC7 56851
Affinity Capture-MS Homo sapiens
102 TMEM120B  
Affinity Capture-MS Homo sapiens
103 EMC4 51234
Affinity Capture-MS Homo sapiens
104 PTDSS1 9791
Affinity Capture-MS Homo sapiens
105 DLK1  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
106 AGPAT3 56894
Affinity Capture-MS Homo sapiens
107 DDX58 23586
Affinity Capture-RNA Homo sapiens
108 MMGT1 93380
Affinity Capture-MS Homo sapiens
109 UFL1 23376
Affinity Capture-MS Homo sapiens
110 SPCS1 28972
Affinity Capture-MS Homo sapiens
111 BFSP1  
Affinity Capture-MS Homo sapiens
112 TMEM38B  
Affinity Capture-MS Homo sapiens
113 CDK5RAP3 80279
Affinity Capture-MS Homo sapiens
114 EI24  
Affinity Capture-MS Homo sapiens
115 STT3B 201595
Affinity Capture-MS Homo sapiens
116 SGCZ  
Affinity Capture-MS Homo sapiens
117 C9orf72  
Affinity Capture-MS Homo sapiens
118 FPR2  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
119 CALR3  
Proximity Label-MS Homo sapiens
120 SPCS2 9789
Affinity Capture-MS Homo sapiens
121 RPL17 6139
Affinity Capture-Western Homo sapiens
122 EP300 2033
Affinity Capture-MS Homo sapiens
123 DERL1 79139
Affinity Capture-MS Homo sapiens
View the network image/svg+xml
 Pathways in which NCLN is involved
No pathways found





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