Gene description for LBR
Gene name lamin B receptor
Gene symbol LBR
Other names/aliases DHCR14B
LMN2R
PHA
TDRD18
Species Homo sapiens
 Database cross references - LBR
ExoCarta ExoCarta_3930
Entrez Gene 3930
HGNC 6518
MIM 600024
UniProt Q14739  
 LBR identified in exosomes derived from the following tissue/cell type
Hepatocellular carcinoma cells 26054723    
Squamous carcinoma cells 20124223    
 Gene ontology annotations for LBR
Molecular Function
    protein binding GO:0005515 IPI
    lamin binding GO:0005521 TAS
    poly(A) RNA binding GO:0044822 IDA
    oxidoreductase activity, acting on the CH-CH group of donors, NAD or NADP as acceptor GO:0016628 IEA
    DNA binding GO:0003677 IEA
    chromo shadow domain binding GO:0070087 IPI
Biological Process
    cholesterol biosynthetic process GO:0006695 TAS
    oxidation-reduction process GO:0055114 IEA
    small molecule metabolic process GO:0044281 TAS
Subcellular Localization
    nuclear envelope GO:0005635 TAS
    integral component of nuclear inner membrane GO:0005639 TAS
    nuclear membrane GO:0031965 IDA
    membrane GO:0016020 IDA
    integral component of membrane GO:0016021 IDA
 Experiment description of studies that identified LBR in exosomes
1
Experiment ID 235
ISEV standards
✔
EM
EV Biophysical techniques
✔
TSG101|Alix|HSC70|GAPDH
EV Cytosolic markers
✘ EV Membrane markers
✔
HSP90B1
EV Negative markers
✔
qNano
EV Particle analysis
Identified molecule mRNA
Identification method RNA Sequencing
PubMed ID 26054723    
Organism Homo sapiens
Experiment description Hepatocellular carcinoma-derived exosomes promote motility of immortalized hepatocyte through transfer of oncogenic proteins and RNAs
Authors He M, Qin H, Poon TC, Sze SC, Ding X, Co NN, Ngai SM, Chan TF, Wong N
Journal name Carcinogenesis
Publication year 2015
Sample Hepatocellular carcinoma cells
Sample name HKCI-8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
RNA
Methods used in the study Western blotting
Mass spectrometry
RT-PCR
RNA Sequencing
2
Experiment ID 191
ISEV standards
✘ EV Biophysical techniques
✔
Alix
EV Cytosolic markers
✔
CD81|CD9
EV Membrane markers
✘ EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 20124223    
Organism Homo sapiens
Experiment description Hypoxic tumor cell modulates its microenvironment to enhance angiogenic and metastatic potential by secretion of proteins and exosomes.
Authors Park JE, Tan HS, Datta A, Lai RC, Zhang H, Meng W, Lim SK, Sze SK.
Journal name Mol Cell Proteomics
Publication year 2010
Sample Squamous carcinoma cells
Sample name Squamous carcinoma cell (A431)
Isolation/purification methods Differential centrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for LBR
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 AKAP8L  
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
2 CBX3 11335
Invivo Homo sapiens
Invitro Homo sapiens
Two-hybrid Homo sapiens
3 FNBP4  
Invitro Homo sapiens
Affinity Capture-MS Homo sapiens
4 YWHAG 7532
Affinity Capture-MS Homo sapiens
5 CBX5 23468
Invivo Homo sapiens
Invitro Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-MS Homo sapiens
6 LMNB1 4001
Invivo Homo sapiens
Affinity Capture-MS Homo sapiens
View the network image/svg+xml
 Pathways in which LBR is involved
PathwayEvidenceSource
Cholesterol biosynthesis TAS Reactome





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