Gene description for NET1
Gene name neuroepithelial cell transforming 1
Gene symbol NET1
Other names/aliases ARHGEF8
NET1A
Species Homo sapiens
 Database cross references - NET1
ExoCarta ExoCarta_10276
Entrez Gene 10276
HGNC 14592
MIM 606450
UniProt Q7Z628  
 NET1 identified in exosomes derived from the following tissue/cell type
Hepatocellular carcinoma cells 26054723    
Hepatocellular carcinoma cells 26054723    
Hepatocytes 26054723    
 Gene ontology annotations for NET1
Molecular Function
    GTP-Rho binding GO:0017049 IEA
    guanyl-nucleotide exchange factor activity GO:0005085 TAS
    Rho guanyl-nucleotide exchange factor activity GO:0005089 IEA
Biological Process
    positive regulation of substrate adhesion-dependent cell spreading GO:1900026 IEA
    small GTPase mediated signal transduction GO:0007264 TAS
    apoptotic signaling pathway GO:0097190 TAS
    cellular response to ionizing radiation GO:0071479 IDA
    signal transduction GO:0007165 TAS
    neurotrophin TRK receptor signaling pathway GO:0048011 TAS
    cellular response to hydrogen peroxide GO:0070301 IDA
    regulation of cell growth GO:0001558 NAS
    myoblast migration GO:0051451 IEA
    positive regulation of apoptotic process GO:0043065 TAS
    positive regulation of GTPase activity GO:0043547 IMP
    regulation of small GTPase mediated signal transduction GO:0051056 TAS
Subcellular Localization
    cytosol GO:0005829 TAS
    nucleus GO:0005634 IEA
    plasma membrane GO:0005886 IEA
 Experiment description of studies that identified NET1 in exosomes
1
Experiment ID 235
ISEV standards
✔
EM
EV Biophysical techniques
✔
TSG101|Alix|HSC70|GAPDH
EV Cytosolic markers
✘ EV Membrane markers
✔
HSP90B1
EV Negative markers
✔
qNano
EV Particle analysis
Identified molecule mRNA
Identification method RNA Sequencing
PubMed ID 26054723    
Organism Homo sapiens
Experiment description Hepatocellular carcinoma-derived exosomes promote motility of immortalized hepatocyte through transfer of oncogenic proteins and RNAs
Authors He M, Qin H, Poon TC, Sze SC, Ding X, Co NN, Ngai SM, Chan TF, Wong N
Journal name Carcinogenesis
Publication year 2015
Sample Hepatocellular carcinoma cells
Sample name HKCI-8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
RNA
Methods used in the study Western blotting
Mass spectrometry
RT-PCR
RNA Sequencing
2
Experiment ID 236
ISEV standards
✔
EM
EV Biophysical techniques
✔
TSG101|Alix|HSC70|GAPDH
EV Cytosolic markers
✘ EV Membrane markers
✔
HSP90B1
EV Negative markers
✔
qNano
EV Particle analysis
Identified molecule mRNA
Identification method RNA Sequencing
PubMed ID 26054723    
Organism Homo sapiens
Experiment description Hepatocellular carcinoma-derived exosomes promote motility of immortalized hepatocyte through transfer of oncogenic proteins and RNAs
Authors He M, Qin H, Poon TC, Sze SC, Ding X, Co NN, Ngai SM, Chan TF, Wong N
Journal name Carcinogenesis
Publication year 2015
Sample Hepatocellular carcinoma cells
Sample name MHCC97L
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
RNA
Methods used in the study Western blotting
Mass spectrometry
RT-PCR
RNA Sequencing
3
Experiment ID 237
ISEV standards
✔
EM
EV Biophysical techniques
✔
TSG101|Alix|HSC70|GAPDH
EV Cytosolic markers
✘ EV Membrane markers
✔
HSP90B1
EV Negative markers
✔
qNano
EV Particle analysis
Identified molecule mRNA
Identification method RNA Sequencing
PubMed ID 26054723    
Organism Homo sapiens
Experiment description Hepatocellular carcinoma-derived exosomes promote motility of immortalized hepatocyte through transfer of oncogenic proteins and RNAs
Authors He M, Qin H, Poon TC, Sze SC, Ding X, Co NN, Ngai SM, Chan TF, Wong N
Journal name Carcinogenesis
Publication year 2015
Sample Hepatocytes
Sample name MIHA
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
RNA
Methods used in the study Western blotting
Mass spectrometry
RNA Sequencing
 Protein-protein interactions for NET1
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 RHOA 387
Two-hybrid Homo sapiens
2 MAGI1  
Affinity Capture-Western Homo sapiens
View the network image/svg+xml
 Pathways in which NET1 is involved
PathwayEvidenceSource
G alpha (12/13) signalling events TAS Reactome
NRAGE signals death through JNK TAS Reactome
Rho GTPase cycle TAS Reactome





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