Gene description for MAGI3
Gene name membrane associated guanylate kinase, WW and PDZ domain containing 3
Gene symbol MAGI3
Other names/aliases MAGI-3
dJ730K3.2
Species Homo sapiens
 Database cross references - MAGI3
ExoCarta ExoCarta_260425
Vesiclepedia VP_260425
Entrez Gene 260425
HGNC 29647
MIM 615943
UniProt Q5TCQ9  
 MAGI3 identified in exosomes derived from the following tissue/cell type
Hepatocellular carcinoma cells 26054723    
Urine 19056867    
 Gene ontology annotations for MAGI3
Molecular Function
    guanylate kinase activity GO:0004385 NAS
    protein binding GO:0005515 IPI
    ATP binding GO:0005524 IEA
Biological Process
    apoptotic process GO:0006915 NAS
    signal transduction GO:0007165 IBA
    intracellular signal transduction GO:0035556 NAS
    GMP metabolic process GO:0046037 IEA
    GDP metabolic process GO:0046710 IEA
Subcellular Localization
    nucleus GO:0005634 IEA
    cytoplasm GO:0005737 IBA
    plasma membrane GO:0005886 IEA
    cell-cell junction GO:0005911 IBA
    bicellular tight junction GO:0005923 IEA
    membrane GO:0016020 NAS
    cell junction GO:0030054 IDA
 Experiment description of studies that identified MAGI3 in exosomes
1
Experiment ID 235
MISEV standards
EM
Biophysical techniques
TSG101|Alix|HSC70|GAPDH
Enriched markers
HSP90B1
Negative markers
qNano
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 26054723    
Organism Homo sapiens
Experiment description Hepatocellular carcinoma-derived exosomes promote motility of immortalized hepatocyte through transfer of oncogenic proteins and RNAs
Authors "He M, Qin H, Poon TC, Sze SC, Ding X, Co NN, Ngai SM, Chan TF, Wong N"
Journal name Carcinogenesis
Publication year 2015
Sample Hepatocellular carcinoma cells
Sample name HKCI-8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
RNA
Methods used in the study Western blotting
Mass spectrometry
RT-PCR
RNA Sequencing
2
Experiment ID 63
MISEV standards
Biophysical techniques
AQP2
Enriched markers
Negative markers
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 19056867    
Organism Homo sapiens
Experiment description Large-scale proteomics and phosphoproteomics of urinary exosomes.
Authors "Gonzales PA, Pisitkun T, Hoffert JD, Tchapyjnikov D, Star RA, Kleta R, Wang NS, Knepper MA"
Journal name JASN
Publication year 2009
Sample Urine
Sample name Urine - Normal
Isolation/purification methods Differential centrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [LTQ]
Western blotting
 Protein-protein interactions for MAGI3
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 PCBP2 5094
Co-fractionation Homo sapiens
2 NET1 10276
Affinity Capture-MS Homo sapiens
3 PRRG4  
Reconstituted Complex Homo sapiens
4 SSTR3  
Affinity Capture-MS Homo sapiens
5 HSPA5 3309
Cross-Linking-MS (XL-MS) Homo sapiens
6 NRCAM  
Two-hybrid Homo sapiens
7 USP43  
Affinity Capture-MS Homo sapiens
8 TP53 7157
Negative Genetic Homo sapiens
9 MDH2 4191
Cross-Linking-MS (XL-MS) Homo sapiens
10 PARD3 56288
Proximity Label-MS Homo sapiens
11 PLXNB3 5365
Two-hybrid Homo sapiens
12 LDLRAD4  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
13 PTEN 5728
Affinity Capture-Western Homo sapiens
Two-hybrid Homo sapiens
Reconstituted Complex Homo sapiens
14 TNK2 10188
Reconstituted Complex Homo sapiens
15 LOC254896  
Affinity Capture-MS Homo sapiens
16 DLGAP4 22839
Affinity Capture-MS Homo sapiens
17 LATS1  
Protein-peptide Homo sapiens
18 DSCAML1  
Two-hybrid Homo sapiens
19 RPE  
Co-fractionation Homo sapiens
20 TCL1B  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
21 CDH1 999
Proximity Label-MS Homo sapiens
22 RAPGEF2 9693
Affinity Capture-MS Homo sapiens
23 TFAM 7019
Cross-Linking-MS (XL-MS) Homo sapiens
24 CIT 11113
Affinity Capture-MS Homo sapiens
25 PTPRB 5787
Two-hybrid Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
26 RHBDL1  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
27 BICD2 23299
Proximity Label-MS Homo sapiens
28 FERMT1 55612
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
29 PTRF 284119
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
30 MED28  
Affinity Capture-Western Homo sapiens
31 PIN4 5303
Cross-Linking-MS (XL-MS) Homo sapiens
32 BAI1  
Protein-peptide Homo sapiens
Two-hybrid Homo sapiens
33 PTPN14 5784
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
View the network image/svg+xml
 Pathways in which MAGI3 is involved
No pathways found





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