Gene description for MAGI3
Gene name membrane associated guanylate kinase, WW and PDZ domain containing 3
Gene symbol MAGI3
Other names/aliases MAGI-3
dJ730K3.2
Species Homo sapiens
 Database cross references - MAGI3
ExoCarta ExoCarta_260425
Entrez Gene 260425
HGNC 29647
MIM 615943
UniProt Q5TCQ9  
 MAGI3 identified in exosomes derived from the following tissue/cell type
Hepatocellular carcinoma cells 26054723    
Urine 19056867    
 Gene ontology annotations for MAGI3
Molecular Function
    ATP binding GO:0005524 IEA
    frizzled binding GO:0005109 IEA
    protein complex scaffold GO:0032947 IEA
    protein binding GO:0005515 IPI
    guanylate kinase activity GO:0004385 NAS
Biological Process
    nucleotide phosphorylation GO:0046939 NAS
    apoptotic process GO:0006915 NAS
    intracellular signal transduction GO:0035556 NAS
    viral process GO:0016032 IEA
    positive regulation of JUN kinase activity GO:0043507 IEA
Subcellular Localization
    membrane GO:0016020 NAS
    nucleus GO:0005634 IEA
    bicellular tight junction GO:0005923 IEA
    plasma membrane GO:0005886 IEA
 Experiment description of studies that identified MAGI3 in exosomes
1
Experiment ID 235
ISEV standards
EM
EV Biophysical techniques
TSG101|Alix|HSC70|GAPDH
EV Cytosolic markers
EV Membrane markers
HSP90B1
EV Negative markers
qNano
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 26054723    
Organism Homo sapiens
Experiment description Hepatocellular carcinoma-derived exosomes promote motility of immortalized hepatocyte through transfer of oncogenic proteins and RNAs
Authors He M, Qin H, Poon TC, Sze SC, Ding X, Co NN, Ngai SM, Chan TF, Wong N
Journal name Carcinogenesis
Publication year 2015
Sample Hepatocellular carcinoma cells
Sample name HKCI-8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
RNA
Methods used in the study Western blotting
Mass spectrometry
RT-PCR
RNA Sequencing
2
Experiment ID 63
ISEV standards
EV Biophysical techniques
EV Cytosolic markers
AQP2
EV Membrane markers
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 19056867    
Organism Homo sapiens
Experiment description Large-scale proteomics and phosphoproteomics of urinary exosomes.
Authors Gonzales PA, Pisitkun T, Hoffert JD, Tchapyjnikov D, Star RA, Kleta R, Wang NS, Knepper MA
Journal name JASN
Publication year 2009
Sample Urine
Sample name Urine - Normal
Isolation/purification methods Differential centrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [LTQ]
Western blotting
 Protein-protein interactions for MAGI3
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 PTPRB 5787
Two-hybrid Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
2 PTEN  
Affinity Capture-Western Homo sapiens
Two-hybrid Homo sapiens
Reconstituted Complex Homo sapiens
3 BAI1  
Two-hybrid Homo sapiens
View the network image/svg+xml
 Pathways in which MAGI3 is involved
No pathways found





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