Gene description for RAPGEF2
Gene name Rap guanine nucleotide exchange factor (GEF) 2
Gene symbol RAPGEF2
Other names/aliases CNrasGEF
NRAPGEP
PDZ-GEF1
PDZGEF1
RA-GEF
RA-GEF-1
Rap-GEP
nRap GEP
Species Homo sapiens
 Database cross references - RAPGEF2
ExoCarta ExoCarta_9693
Vesiclepedia VP_9693
Entrez Gene 9693
HGNC 16854
MIM 609530
UniProt Q9Y4G8  
 RAPGEF2 identified in exosomes derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Mesenchymal stem cells 36408942    
 Gene ontology annotations for RAPGEF2
Molecular Function
    guanyl-nucleotide exchange factor activity GO:0005085 IBA
    guanyl-nucleotide exchange factor activity GO:0005085 IDA
    GTPase activator activity GO:0005096 IDA
    calcium ion binding GO:0005509 NAS
    protein binding GO:0005515 IPI
    diacylglycerol binding GO:0019992 NAS
    PDZ domain binding GO:0030165 IDA
    cAMP binding GO:0030552 IDA
    cAMP binding GO:0030552 IDA
    cGMP binding GO:0030553 IDA
    beta-1 adrenergic receptor binding GO:0031697 IDA
    WW domain binding GO:0050699 IDA
    phosphatidic acid binding GO:0070300 IDA
Biological Process
    MAPK cascade GO:0000165 NAS
    blood vessel development GO:0001568 ISS
    neuron migration GO:0001764 ISS
    G protein-coupled receptor signaling pathway GO:0007186 IDA
    adenylate cyclase-modulating G protein-coupled receptor signaling pathway GO:0007188 IDA
    neuropeptide signaling pathway GO:0007218 IDA
    small GTPase-mediated signal transduction GO:0007264 TAS
    Ras protein signal transduction GO:0007265 IBA
    negative regulation of cell population proliferation GO:0008285 IDA
    positive regulation of neuron projection development GO:0010976 ISS
    cAMP-mediated signaling GO:0019933 NAS
    ventricular system development GO:0021591 ISS
    forebrain neuron development GO:0021884 ISS
    microvillus assembly GO:0030033 IGI
    neuron projection development GO:0031175 IDA
    brain-derived neurotrophic factor receptor signaling pathway GO:0031547 ISS
    positive regulation of protein binding GO:0032092 ISS
    Rap protein signal transduction GO:0032486 IMP
    intracellular signal transduction GO:0035556 TAS
    nerve growth factor signaling pathway GO:0038180 ISS
    positive regulation of GTPase activity GO:0043547 IDA
    positive regulation of GTPase activity GO:0043547 IMP
    positive regulation of protein kinase activity GO:0045860 IDA
    negative regulation of melanin biosynthetic process GO:0048022 ISS
    regulation of synaptic plasticity GO:0048167 ISS
    negative regulation of dendrite morphogenesis GO:0050774 IDA
    establishment of endothelial barrier GO:0061028 IMP
    positive regulation of ERK1 and ERK2 cascade GO:0070374 IDA
    cellular response to cAMP GO:0071320 IDA
    cellular response to cGMP GO:0071321 IDA
    adenylate cyclase-activating adrenergic receptor signaling pathway GO:0071880 IDA
    protein localization to plasma membrane GO:0072659 IMP
    establishment of endothelial intestinal barrier GO:0090557 IMP
    regulation of cell junction assembly GO:1901888 IMP
    cellular response to nerve growth factor stimulus GO:1990090 ISS
    positive regulation of cAMP-dependent protein kinase activity GO:2000481 IDA
    positive regulation of dendritic cell apoptotic process GO:2000670 IDA
    positive regulation of vasculogenesis GO:2001214 ISS
    positive regulation of neuron migration GO:2001224 ISS
Subcellular Localization
    cytoplasm GO:0005737 IDA
    late endosome GO:0005770 IDA
    cytosol GO:0005829 IDA
    cytosol GO:0005829 TAS
    plasma membrane GO:0005886 IBA
    plasma membrane GO:0005886 IDA
    plasma membrane GO:0005886 ISS
    plasma membrane GO:0005886 NAS
    cell-cell junction GO:0005911 ISS
    bicellular tight junction GO:0005923 IDA
    membrane GO:0016020 IDA
    apical plasma membrane GO:0016324 IBA
    apical plasma membrane GO:0016324 IDA
    endocytic vesicle GO:0030139 IBA
    endocytic vesicle GO:0030139 IDA
    protein-containing complex GO:0032991 ISS
    neuron projection GO:0043005 ISS
    neuronal cell body GO:0043025 ISS
    synapse GO:0045202 ISS
    perinuclear region of cytoplasm GO:0048471 IDA
 Experiment description of studies that identified RAPGEF2 in exosomes
1
Experiment ID 489
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
5
Experiment ID 488
MISEV standards
✔
EM
Biophysical techniques
✔
CD9|CD81|CD63|GAPDH|SDCBP|LAMP1|TFRC|UCHL1|FLOT2|LAMP2|FLOT1|ICAM1|RAB5B|CD151|RAB35|TSG101|RAB5A|CD82
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Homo sapiens
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Mesenchymal stem cells
Sample name UCMSC
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
 Protein-protein interactions for RAPGEF2
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 YWHAH 7533
Affinity Capture-MS Homo sapiens
2 UBE2H 7328
Affinity Capture-MS Homo sapiens
3 COX5A 9377
Affinity Capture-MS Homo sapiens
4 RAP2B 5912
Reconstituted Complex Homo sapiens
5 WDR91 29062
Affinity Capture-MS Homo sapiens
6 MESDC2 23184
Affinity Capture-MS Homo sapiens
7 SNX27 81609
Affinity Capture-MS Homo sapiens
8 BTRC 8945
Affinity Capture-Western Homo sapiens
Co-localization Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
9 FBXW11  
Affinity Capture-Western Homo sapiens
Co-localization Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
10 HMCN1 83872
Affinity Capture-MS Homo sapiens
11 KCTD17 79734
Affinity Capture-MS Homo sapiens
12 TNKS 8658
Affinity Capture-MS Homo sapiens
13 PLEC 5339
Affinity Capture-MS Homo sapiens
14 NEDD4 4734
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
15 RALGDS  
Reconstituted Complex Homo sapiens
Two-hybrid Homo sapiens
16 XPO1 7514
Affinity Capture-MS Homo sapiens
17 YWHAB 7529
Affinity Capture-MS Homo sapiens
18 WWOX 51741
Affinity Capture-MS Homo sapiens
19 RACGAP1 29127
Affinity Capture-MS Homo sapiens
20 SPP1 6696
Two-hybrid Homo sapiens
21 YWHAE 7531
Affinity Capture-MS Homo sapiens
22 LAP3 51056
Affinity Capture-MS Homo sapiens
23 HTT 3064
Affinity Capture-MS Homo sapiens
24 ANO3  
Affinity Capture-MS Homo sapiens
25 EPB41L5 57669
Affinity Capture-MS Homo sapiens
26 BRD8 10902
Affinity Capture-MS Homo sapiens
27 TJP1 7082
Affinity Capture-MS Homo sapiens
28 LMNA 4000
Affinity Capture-MS Homo sapiens
29 YWHAZ 7534
Affinity Capture-MS Homo sapiens
30 VSIG8  
Affinity Capture-MS Homo sapiens
31 HIST2H4B 554313
Affinity Capture-MS Homo sapiens
32 UBC 7316
Affinity Capture-Western Homo sapiens
33 MAGI1  
Affinity Capture-MS Homo sapiens
34 TRMT6 51605
Affinity Capture-MS Homo sapiens
35 KIF5B 3799
Two-hybrid Homo sapiens
36 SND1 27044
Affinity Capture-MS Homo sapiens
37 STOM 2040
Affinity Capture-MS Homo sapiens
38 MAGEA9  
Affinity Capture-MS Homo sapiens
39 SEC62 7095
Affinity Capture-MS Homo sapiens
40 MOB4 25843
Two-hybrid Homo sapiens
41 CCDC132 55610
Affinity Capture-MS Homo sapiens
42 SCYL2 55681
Affinity Capture-MS Homo sapiens
43 USP11 8237
Affinity Capture-MS Homo sapiens
44 PRKCI 5584
Affinity Capture-MS Homo sapiens
45 Msn 17698
Affinity Capture-MS Mus musculus
46 SLC25A11 8402
Affinity Capture-MS Homo sapiens
47 BDP1  
Affinity Capture-MS Homo sapiens
48 KIF5A 3798
Two-hybrid Homo sapiens
49 MAGI3 260425
Affinity Capture-MS Homo sapiens
50 USMG5 84833
Affinity Capture-MS Homo sapiens
51 YAP1 10413
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
52 SREK1IP1  
Affinity Capture-MS Homo sapiens
53 TTN 7273
Affinity Capture-MS Homo sapiens
54 UHRF1BP1 54887
Affinity Capture-MS Homo sapiens
55 PRPSAP1 5635
Affinity Capture-MS Homo sapiens
56 XIAP  
Affinity Capture-MS Homo sapiens
57 SSSCA1 10534
Affinity Capture-MS Homo sapiens
58 RAP1A 5906
Reconstituted Complex Homo sapiens
Biochemical Activity Homo sapiens
59 HSPA1A 3303
Two-hybrid Homo sapiens
60 FLNC 2318
Affinity Capture-MS Homo sapiens
61 KIF11 3832
Affinity Capture-MS Homo sapiens
62 IL16  
Two-hybrid Homo sapiens
63 KCTD5 54442
Affinity Capture-MS Homo sapiens
64 ZNF512B  
Affinity Capture-MS Homo sapiens
65 YWHAG 7532
Affinity Capture-MS Homo sapiens
66 RAPGEF6 51735
Affinity Capture-MS Homo sapiens
67 PTPN13 5783
Affinity Capture-MS Homo sapiens
68 ATP8  
Two-hybrid Homo sapiens
69 ERI3 79033
Affinity Capture-MS Homo sapiens
70 LRR1  
Affinity Capture-MS Homo sapiens
71 CTTNBP2  
Affinity Capture-MS Homo sapiens
72 SP140L  
Affinity Capture-MS Homo sapiens
View the network image/svg+xml
 Pathways in which RAPGEF2 is involved
PathwayEvidenceSource
MAPK family signaling cascades TAS Reactome
MAPK1/MAPK3 signaling TAS Reactome
RAF/MAP kinase cascade TAS Reactome
Signal Transduction TAS Reactome





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