ExoCarta: Gene summary

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Gene description for SPP1
Gene name	secreted phosphoprotein 1
Gene symbol	SPP1
Other names/aliases	BNSP BSPI ETA-1 OPN
Species	Homo sapiens
Database cross references - SPP1
ExoCarta	ExoCarta_6696
Vesiclepedia	VP_6696
Entrez Gene	6696
HGNC	11255
MIM	166490
UniProt	P10451
SPP1 identified in exosomes derived from the following tissue/cell type
Bone marrow mesenchymal stem cells	36408942
Bone marrow mesenchymal stem cells	36408942
Bone marrow mesenchymal stem cells	36408942
Bone marrow mesenchymal stem cells	36408942
Ovarian cancer cells	23333927
Ovarian cancer cells	23333927

Gene ontology annotations for SPP1

Molecular Function
	cytokine activity	GO:0005125	IEA
	integrin binding	GO:0005178	IPI
	protein binding	GO:0005515	IPI
	small molecule binding	GO:0036094	EXP
	extracellular matrix binding	GO:0050840	IBA
Biological Process
	osteoblast differentiation	GO:0001649	IBA
	androgen catabolic process	GO:0006710	IDA
	cell adhesion	GO:0007155	IBA
	cell adhesion	GO:0007155	IDA
	signal transduction	GO:0007165	IEA
	embryo implantation	GO:0007566	TAS
	biomineral tissue development	GO:0031214	IEA
	response to vitamin D	GO:0033280	IDA
	response to macrophage colony-stimulating factor	GO:0036005	IEA
	positive regulation of bone resorption	GO:0045780	IBA
	positive regulation of DNA-templated transcription	GO:0045893	IDA
	decidualization	GO:0046697	TAS
	response to steroid hormone	GO:0048545	IEA
	negative regulation of collateral sprouting of intact axon in response to injury	GO:0048685	IEA
	cellular response to testosterone stimulus	GO:0071394	IDA
	response to 2,3,7,8-tetrachlorodibenzodioxine	GO:1904612	IEA
	positive regulation of estradiol secretion	GO:2000866	IDA
Subcellular Localization
	extracellular region	GO:0005576	TAS
	extracellular space	GO:0005615	HDA
	extracellular space	GO:0005615	IBA
	extracellular space	GO:0005615	IDA
	endoplasmic reticulum lumen	GO:0005788	TAS
	Golgi apparatus	GO:0005794	IDA
	cell projection	GO:0042995	IEA
	perinuclear region of cytoplasm	GO:0048471	IEA
	extracellular exosome	GO:0070062	HDA

Experiment description of studies that identified SPP1 in exosomes

Experiment ID

489

MISEV standards
✔ EM	Biophysical techniques
✔ Cd9\|Cd81\|Cd63\|Gapdh\|Sdcbp\|Lamp1\|Aqp1\|Rab5a\|Icam1\|Cd82\|Itga2b\|Tsg101\|Lamp2\|Rab35\|Flot1\|Flot2\|Cd151\|Rab5b\|Tfrc\|Uchl1	Enriched markers
✔ Canx	Negative markers
✔ NTA	Particle analysis

Identified molecule

Protein

Identification method

Mass spectrometry

PubMed ID

36408942

Organism

Rattus norvegicus

Experiment description

Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells

Authors

"Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."

Journal name

Proteomics

Publication year

2023

Sample

Bone marrow mesenchymal stem cells

Sample name

BMSC - Passage 6

Isolation/purification methods

Differential centrifugation
Filtration
Ultracentrifugation

Flotation density

Molecules identified in the study

Protein

Methods used in the study

Western blotting
Mass spectometry

Experiment ID

490

MISEV standards
✔ EM	Biophysical techniques
✔ Cd9\|Cd81\|Cd63\|Gapdh\|Sdcbp\|Lamp1\|Aqp1\|Rab5a\|Icam1\|Cd82\|Itga2b\|Tsg101\|Lamp2\|Rab35\|Flot1\|Flot2\|Cd151\|Rab5b\|Tfrc\|Uchl1	Enriched markers
✔ Canx	Negative markers
✔ NTA	Particle analysis

Identified molecule

Protein

Identification method

Mass spectrometry

PubMed ID

36408942

Organism

Rattus norvegicus

Experiment description

Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells

Authors

"Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."

Journal name

Proteomics

Publication year

2023

Sample

Bone marrow mesenchymal stem cells

Sample name

BMSC - Passage 7

Isolation/purification methods

Differential centrifugation
Filtration
Ultracentrifugation

Flotation density

Molecules identified in the study

Protein

Methods used in the study

Western blotting
Mass spectometry

Experiment ID

491

MISEV standards
✔ EM	Biophysical techniques
✔ Cd9\|Cd81\|Cd63\|Gapdh\|Sdcbp\|Lamp1\|Aqp1\|Rab5a\|Icam1\|Cd82\|Itga2b\|Tsg101\|Lamp2\|Rab35\|Flot1\|Flot2\|Cd151\|Rab5b\|Tfrc\|Uchl1	Enriched markers
✔ Canx	Negative markers
✔ NTA	Particle analysis

Identified molecule

Protein

Identification method

Mass spectrometry

PubMed ID

36408942

Organism

Rattus norvegicus

Experiment description

Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells

Authors

"Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."

Journal name

Proteomics

Publication year

2023

Sample

Bone marrow mesenchymal stem cells

Sample name

BMSC - Passage 8

Isolation/purification methods

Differential centrifugation
Filtration
Ultracentrifugation

Flotation density

Molecules identified in the study

Protein

Methods used in the study

Western blotting
Mass spectometry

Experiment ID

492

MISEV standards
✔ EM	Biophysical techniques
✔ Cd9\|Cd81\|Cd63\|Gapdh\|Sdcbp\|Lamp1\|Aqp1\|Rab5a\|Icam1\|Cd82\|Itga2b\|Tsg101\|Lamp2\|Rab35\|Flot1\|Flot2\|Cd151\|Rab5b\|Tfrc\|Uchl1	Enriched markers
✔ Canx	Negative markers
✔ NTA	Particle analysis

Identified molecule

Protein

Identification method

Mass spectrometry

PubMed ID

36408942

Organism

Rattus norvegicus

Experiment description

Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells

Authors

"Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."

Journal name

Proteomics

Publication year

2023

Sample

Bone marrow mesenchymal stem cells

Sample name

BMSC - Passage 9

Isolation/purification methods

Differential centrifugation
Filtration
Ultracentrifugation

Flotation density

Molecules identified in the study

Protein

Methods used in the study

Western blotting
Mass spectometry

Experiment ID

211

MISEV standards
✔ EM	Biophysical techniques
✔ TSG101\|Alix\|EpCAM\|TFRC	Enriched markers
✔ cytochrome c\|GOLGA2	Negative markers
✘	Particle analysis

Identified molecule

protein

Identification method

Mass spectrometry

PubMed ID

23333927

Organism

Homo sapiens

Experiment description

Characterization and proteomic analysis of ovarian cancer-derived exosomes.

Authors

"Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K."

Journal name

J Proteomics

Publication year

2013

Sample

Ovarian cancer cells

Sample name

IGROV1

Isolation/purification methods

Differential centrifugation
Ultracentrifugation
Sucrose density gradient

Flotation density

1.09-1.15 g/mL

Molecules identified in the study

Protein

Methods used in the study

Mass spectrometry

Experiment ID

212

MISEV standards
✔ CEM	Biophysical techniques
✔ TSG101\|Alix\|EpCAM\|TFRC	Enriched markers
✔ Cytochrome C\|GOLGA2	Negative markers
✘	Particle analysis

Identified molecule

protein

Identification method

Mass spectrometry

PubMed ID

23333927

Organism

Homo sapiens

Experiment description

Characterization and proteomic analysis of ovarian cancer-derived exosomes.

Authors

"Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K."

Journal name

J Proteomics

Publication year

2013

Sample

Ovarian cancer cells

Sample name

OVCAR-3

Isolation/purification methods

Differential centrifugation
Ultracentrifugation
Sucrose density gradient

Flotation density

1.09-1.15 g/mL

Molecules identified in the study

Protein

Methods used in the study

Mass spectrometry

Protein-protein interactions for SPP1

Protein Interactor

ExoCarta ID

Identification method

PubMed

Species

ITGB1

3688

Reconstituted Complex

Homo sapiens

MARCKS

4082

Two-hybrid

Homo sapiens

EIF3K

27335

Two-hybrid

Homo sapiens

NACA

4666

Two-hybrid

Homo sapiens

CTNNBL1

56259

Two-hybrid

Homo sapiens

STK39

27347

Two-hybrid

Homo sapiens

C2orf50

Two-hybrid

Homo sapiens

RPL15

6138

Two-hybrid

Homo sapiens

GRIPAP1

56850

Two-hybrid

Homo sapiens

SSB

6741

Two-hybrid

Homo sapiens

CALM1

801

Two-hybrid

Homo sapiens

TBC1D8

Two-hybrid

Homo sapiens

AFF4

27125

Two-hybrid

Homo sapiens

TMEM168

64418

Two-hybrid

Homo sapiens

ZNF638

27332

Two-hybrid

Homo sapiens

MRPL23

6150

Two-hybrid

Homo sapiens

SEC24C

9632

Two-hybrid

Homo sapiens

COPS5

10987

Two-hybrid

Homo sapiens

RIMS4

Two-hybrid

Homo sapiens

USP42

Two-hybrid

Homo sapiens

DNAJB1

3337

Two-hybrid

Homo sapiens

CEP162

Two-hybrid

Homo sapiens

PEPD

5184

Two-hybrid

Homo sapiens

UBQLN4

56893

Two-hybrid		Homo sapiens
Two-hybrid		Homo sapiens

NCSTN

23385

Two-hybrid

Homo sapiens

EEF1A1

1915

Two-hybrid

Homo sapiens

TPT1

7178

Two-hybrid

Homo sapiens

CHD1

1105

Two-hybrid

Homo sapiens

AMOTL2

51421

Two-hybrid

Homo sapiens

CC2D1B

200014

Two-hybrid

Homo sapiens

IGFBP5

3488

Reconstituted Complex

Homo sapiens

MAP1B

4131

Two-hybrid

Homo sapiens

STAU1

6780

Two-hybrid

Homo sapiens

PEMT

Two-hybrid

Homo sapiens

BAG6

7917

Two-hybrid		Homo sapiens
Two-hybrid		Homo sapiens

MPPED1

Two-hybrid

Homo sapiens

ASNA1

439

Two-hybrid

Homo sapiens

USP8

9101

Two-hybrid

Homo sapiens

HMGN1

Two-hybrid

Homo sapiens

MEOX2

Two-hybrid

Homo sapiens

NCS1

23413

Two-hybrid

Homo sapiens

RPL5

6125

Two-hybrid

Homo sapiens

DNTTIP2

Two-hybrid

Homo sapiens

PRKACB

5567

Two-hybrid

Homo sapiens

CACNG4

Two-hybrid

Homo sapiens

RBBP6

5930

Two-hybrid

Homo sapiens

TUBB

203068

Two-hybrid

Homo sapiens

TMEM30A

55754

Two-hybrid

Homo sapiens

SLC39A6

25800

Two-hybrid

Homo sapiens

EPAS1

Two-hybrid

Homo sapiens

NCKAP1

10787

Two-hybrid

Homo sapiens

MEX3A

Affinity Capture-RNA

Homo sapiens

KCNIP1

Two-hybrid

Homo sapiens

SKP2

Affinity Capture-MS

Homo sapiens

ATP5F1

515

Two-hybrid

Homo sapiens

FTH1

2495

Two-hybrid

Homo sapiens

TRAF3

7187

Affinity Capture-Western		Homo sapiens
Affinity Capture-Western		Homo sapiens

ZNF804A

Two-hybrid

Homo sapiens

RANBP9

10048

Two-hybrid

Homo sapiens

C1RL

51279

Two-hybrid

Homo sapiens

RAPGEF2

9693

Two-hybrid

Homo sapiens

RPS13

6207

Two-hybrid

Homo sapiens

DSEL

Two-hybrid

Homo sapiens

DCX

Two-hybrid

Homo sapiens

SGTA

6449

Two-hybrid		Homo sapiens
Two-hybrid		Homo sapiens
Two-hybrid		Homo sapiens
Two-hybrid		Homo sapiens
Two-hybrid		Homo sapiens

BRD3

8019

Two-hybrid

Homo sapiens

CCR8

Two-hybrid

Homo sapiens

DRD2

Two-hybrid

Homo sapiens

COMMD1

150684

Two-hybrid

Homo sapiens

RPL13

6137

Two-hybrid

Homo sapiens

PDLIM7

9260

Two-hybrid

Homo sapiens

TTC1

7265

Two-hybrid

Homo sapiens

PARP1

142

Affinity Capture-MS

Homo sapiens

HBA2

3040

Two-hybrid

Homo sapiens

CMTM3

123920

Two-hybrid

Homo sapiens

SGTB

Two-hybrid

Homo sapiens

SNAPIN

23557

Two-hybrid

Homo sapiens

LRRC4

Two-hybrid

Homo sapiens

ATP2A2

488

Two-hybrid

Homo sapiens

ITGAV

3685

Reconstituted Complex

Homo sapiens

PLD3

23646

Two-hybrid

Homo sapiens

2147

Co-localization

Homo sapiens

CYB5R3

1727

Two-hybrid

Homo sapiens

CISD2

493856

Two-hybrid

Homo sapiens

MAP1A

4130

Two-hybrid

Homo sapiens

ITGB5

3693

Reconstituted Complex

Homo sapiens

PTGER4

Two-hybrid

Homo sapiens

APOA1

335

Two-hybrid

Homo sapiens

View the network

Pathways in which SPP1 is involved

Pathway	Evidence	Source
Degradation of the extracellular matrix	TAS	Reactome
Extracellular matrix organization	TAS	Reactome
Extracellular matrix organization	IEA	Reactome
Gene expression (Transcription)	TAS	Reactome
Generic Transcription Pathway	TAS	Reactome
Integrin cell surface interactions	TAS	Reactome
Integrin cell surface interactions	IEA	Reactome
Metabolism of proteins	TAS	Reactome
Post-translational protein modification	TAS	Reactome
Post-translational protein phosphorylation	TAS	Reactome
Regulation of Insulin-like Growth Factor (IGF) transport and uptake by Insulin-like Growth Factor Binding Proteins (IGFBPs)	TAS	Reactome
RNA Polymerase II Transcription	TAS	Reactome
RUNX3 Regulates Immune Response and Cell Migration	TAS	Reactome
Signal Transduction	TAS	Reactome
Signaling by PDGF	TAS	Reactome
Signaling by Receptor Tyrosine Kinases	TAS	Reactome
Transcriptional regulation by RUNX3	TAS	Reactome

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