Gene description for RBBP6
Gene name retinoblastoma binding protein 6
Gene symbol RBBP6
Other names/aliases MY038
P2P-R
PACT
RBQ-1
SNAMA
Species Homo sapiens
 Database cross references - RBBP6
ExoCarta ExoCarta_5930
Vesiclepedia VP_5930
Entrez Gene 5930
HGNC 9889
MIM 600938
UniProt Q7Z6E9  
 RBBP6 identified in exosomes derived from the following tissue/cell type
Hepatocellular carcinoma cells 26054723    
Neuroblastoma cells 25944692    
 Gene ontology annotations for RBBP6
Molecular Function
    RNA binding GO:0003723 HDA
    ubiquitin-protein transferase activity GO:0004842 IDA
    protein binding GO:0005515 IPI
    zinc ion binding GO:0008270 IEA
    protein kinase binding GO:0019901 IPI
    ubiquitin protein ligase activity GO:0061630 IBA
    ubiquitin protein ligase activity GO:0061630 IMP
Biological Process
    in utero embryonic development GO:0001701 IEA
    DNA replication GO:0006260 IEA
    regulation of DNA replication GO:0006275 IMP
    mRNA processing GO:0006397 IEA
    ubiquitin-dependent protein catabolic process GO:0006511 IBA
    ubiquitin-dependent protein catabolic process GO:0006511 IDA
    ubiquitin-dependent protein catabolic process GO:0006511 IMP
    DNA damage response GO:0006974 IMP
    protein ubiquitination GO:0016567 IBA
    protein ubiquitination GO:0016567 IEA
    multicellular organism growth GO:0035264 IEA
    embryonic organ development GO:0048568 IEA
    somite development GO:0061053 IEA
Subcellular Localization
    nucleus GO:0005634 IBA
    chromosome GO:0005694 IEA
    nucleolus GO:0005730 IEA
    centrosome GO:0005813 IDA
    cytosol GO:0005829 TAS
    nuclear speck GO:0016607 IDA
    protein-containing complex GO:0032991 IDA
 Experiment description of studies that identified RBBP6 in exosomes
1
Experiment ID 235
MISEV standards
EM
Biophysical techniques
TSG101|Alix|HSC70|GAPDH
Enriched markers
HSP90B1
Negative markers
qNano
Particle analysis
Identified molecule mRNA
Identification method RNA Sequencing
PubMed ID 26054723    
Organism Homo sapiens
Experiment description Hepatocellular carcinoma-derived exosomes promote motility of immortalized hepatocyte through transfer of oncogenic proteins and RNAs
Authors "He M, Qin H, Poon TC, Sze SC, Ding X, Co NN, Ngai SM, Chan TF, Wong N"
Journal name Carcinogenesis
Publication year 2015
Sample Hepatocellular carcinoma cells
Sample name HKCI-8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
RNA
Methods used in the study Western blotting
Mass spectrometry
RT-PCR
RNA Sequencing
2
Experiment ID 224
MISEV standards
EM|AFM
Biophysical techniques
Alix|TSG101|CD63|CD81
Enriched markers
GOLGA2
Negative markers
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25944692    
Organism Homo sapiens
Experiment description Proteogenomic analysis reveals exosomes are more oncogenic than ectosomes
Authors "Keerthikumar S, Gangoda L, Liem M, Fonseka P, Atukorala I, Ozcitti C, Mechler A, Adda CG, Ang CS, Mathivanan S"
Journal name Oncotarget
Publication year 2015
Sample Neuroblastoma cells
Sample name SH-SY5Y
Isolation/purification methods Differential centrifugation
Ultracentrifugation
OptiPrep density gradient
Flotation density 1.10 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
Western blotting
 Protein-protein interactions for RBBP6
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 UBE2H 7328
Affinity Capture-MS Homo sapiens
2 ZBTB38 253461
Two-hybrid Homo sapiens
3 SLFN11 91607
Affinity Capture-MS Homo sapiens
4 RIT1 6016
Negative Genetic Homo sapiens
5 H2AFJ 55766
Affinity Capture-MS Homo sapiens
6 HNRNPC 3183
Affinity Capture-MS Homo sapiens
7 Tmed2 56334
Affinity Capture-MS Mus musculus
8 KHDRBS3  
Two-hybrid Homo sapiens
9 ZNF346  
Affinity Capture-MS Homo sapiens
10 WWP1 11059
Affinity Capture-MS Homo sapiens
11 PRPF8 10594
Affinity Capture-MS Homo sapiens
12 SRPK2 6733
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
13 FMR1 2332
Affinity Capture-MS Homo sapiens
14 KLC3  
Affinity Capture-MS Homo sapiens
15 ACTC1 70
Proximity Label-MS Homo sapiens
16 Chmp4b 75608
Affinity Capture-MS Mus musculus
17 DHX8 1659
Proximity Label-MS Homo sapiens
18 Ndc80  
Affinity Capture-MS Mus musculus
19 KIF20A 10112
Affinity Capture-MS Homo sapiens
20 CSTF2 1478
Affinity Capture-Western Homo sapiens
21 CPSF2 53981
Affinity Capture-Western Homo sapiens
22 NLRP2  
Affinity Capture-MS Homo sapiens
23 PLG 5340
Two-hybrid Homo sapiens
24 HSF2  
Affinity Capture-MS Homo sapiens
25 HNRNPUL2 221092
Affinity Capture-MS Homo sapiens
26 LARP4B 23185
Affinity Capture-MS Homo sapiens
27 EIF4ENIF1 56478
Two-hybrid Homo sapiens
28 DDX23 9416
Proximity Label-MS Homo sapiens
29 LRCH4  
Two-hybrid Homo sapiens
30 HP 3240
Two-hybrid Homo sapiens
31 GSPT2 23708
Affinity Capture-MS Homo sapiens
32 RNPS1 10921
Affinity Capture-MS Homo sapiens
33 UFM1 51569
Co-fractionation Homo sapiens
34 DDX24 57062
Co-fractionation Homo sapiens
35 CPSF4  
Affinity Capture-Western Homo sapiens
36 YES1 7525
Affinity Capture-MS Homo sapiens
37 PRC1 9055
Affinity Capture-MS Homo sapiens
38 SRRM2 23524
Affinity Capture-MS Homo sapiens
39 SNRPC 6631
Affinity Capture-MS Homo sapiens
40 FBXW8 26259
Affinity Capture-MS Homo sapiens
41 MAD2L2 10459
Two-hybrid Homo sapiens
42 DDX58 23586
Affinity Capture-RNA Homo sapiens
43 POTEI 653269
Affinity Capture-MS Homo sapiens
44 SRSF3 6428
Affinity Capture-MS Homo sapiens
45 ATG16L1 55054
Affinity Capture-MS Homo sapiens
46 ZNF576  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
47 MECP2 4204
Affinity Capture-MS Homo sapiens
48 CUL1 8454
Affinity Capture-MS Homo sapiens
49 MYCN  
Affinity Capture-MS Homo sapiens
50 APOH 350
Two-hybrid Homo sapiens
51 ZFYVE9  
Two-hybrid Homo sapiens
52 TFIP11  
Two-hybrid Homo sapiens
53 Tmed10 68581
Affinity Capture-MS Mus musculus
54 MZT1  
Affinity Capture-MS Homo sapiens
55 NCOA1  
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
56 CASK 8573
Two-hybrid Homo sapiens
57 CLK2 1196
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
58 RBP4 5950
Two-hybrid Homo sapiens
59 CLK4  
Affinity Capture-MS Homo sapiens
60 PPP5C 5536
Affinity Capture-MS Homo sapiens
61 HIST1H4A 8359
Affinity Capture-MS Homo sapiens
62 ACOT13  
Two-hybrid Homo sapiens
63 PSME1 5720
Co-fractionation Homo sapiens
64 TRA2A 29896
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
65 CAPZB 832
Affinity Capture-MS Homo sapiens
66 ACIN1 22985
Affinity Capture-MS Homo sapiens
67 SRSF6 6431
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
68 PURG  
Affinity Capture-MS Homo sapiens
69 CBX3 11335
Proximity Label-MS Homo sapiens
70 UBE2I 7329
Two-hybrid Homo sapiens
71 SNRPB 6628
Affinity Capture-MS Homo sapiens
72 CSTF3 1479
Affinity Capture-Western Homo sapiens
73 KMT2E  
Affinity Capture-MS Homo sapiens
74 RAD18  
Affinity Capture-MS Homo sapiens
75 CD81 975
Two-hybrid Homo sapiens
76 ARRDC3 57561
Affinity Capture-MS Homo sapiens
77 HECTD1 25831
Affinity Capture-MS Homo sapiens
78 LINC00305  
Two-hybrid Homo sapiens
79 VAV2 7410
Two-hybrid Homo sapiens
80 MAGEB2 4113
Affinity Capture-MS Homo sapiens
81 CDX1  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
82 DDX27 55661
Co-fractionation Homo sapiens
83 BCLAF1 9774
Affinity Capture-MS Homo sapiens
84 CPSF1 29894
Affinity Capture-Western Homo sapiens
85 WBSCR16  
Two-hybrid Homo sapiens
86 FANCD2  
Affinity Capture-MS Homo sapiens
87 CUL3 8452
Affinity Capture-MS Homo sapiens
88 CLK3  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
89 NUBP2 10101
Co-fractionation Homo sapiens
90 RC3H2  
Affinity Capture-MS Homo sapiens
91 Dennd6a  
Affinity Capture-MS Mus musculus
92 PNN 5411
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
93 SRSF4 6429
Affinity Capture-MS Homo sapiens
94 Plk2  
Affinity Capture-MS Mus musculus
95 Smad3  
Affinity Capture-MS Mus musculus
96 NUDT21 11051
Affinity Capture-MS Homo sapiens
97 Itga5 16402
Affinity Capture-MS Mus musculus
98 ERLEC1 27248
Affinity Capture-MS Homo sapiens
99 EGFR 1956
Negative Genetic Homo sapiens
100 SERINC5 256987
Affinity Capture-MS Homo sapiens
101 Max  
Affinity Capture-MS Mus musculus
102 SF3A1 10291
Affinity Capture-MS Homo sapiens
103 YTHDC1  
Affinity Capture-MS Homo sapiens
104 ANAPC16  
Two-hybrid Homo sapiens
105 RPA3 6119
Proximity Label-MS Homo sapiens
106 PCBP1 5093
Affinity Capture-MS Homo sapiens
107 PIAS1 8554
Two-hybrid Homo sapiens
108 MYC  
Affinity Capture-MS Homo sapiens
109 HECW2  
Affinity Capture-MS Homo sapiens
110 ZC3H18  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
111 PABPC5 140886
Affinity Capture-MS Homo sapiens
112 RPS14 6208
Two-hybrid Homo sapiens
113 TOP1 7150
Affinity Capture-MS Homo sapiens
114 ETS1  
Two-hybrid Homo sapiens
115 HSPA4 3308
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
116 EPRS 2058
Affinity Capture-MS Homo sapiens
117 RBM4 5936
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
118 BRD3 8019
Affinity Capture-MS Homo sapiens
119 MDM2  
Affinity Capture-Western Homo sapiens
Biochemical Activity Homo sapiens
120 Sod1 20655
Affinity Capture-MS Mus musculus
121 ATIC 471
Co-fractionation Homo sapiens
122 YBX1 4904
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
Two-hybrid Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
Biochemical Activity Homo sapiens
123 MAPKAPK2 9261
Affinity Capture-MS Homo sapiens
124 LLGL2 3993
Affinity Capture-MS Homo sapiens
125 FBXL6  
Proximity Label-MS Homo sapiens
126 Tubg1 103733
Affinity Capture-MS Mus musculus
127 SMN1 6606
Two-hybrid Homo sapiens
128 GRB2 2885
Two-hybrid Homo sapiens
Affinity Capture-MS Homo sapiens
129 ASGR1 432
Two-hybrid Homo sapiens
130 CPSF6 11052
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
131 ANKRD50 57182
Affinity Capture-MS Homo sapiens
132 FOLR1 2348
Affinity Capture-MS Homo sapiens
133 NEK6 10783
Two-hybrid Homo sapiens
Reconstituted Complex Homo sapiens
Biochemical Activity Homo sapiens
134 SPP1 6696
Two-hybrid Homo sapiens
135 PPIG 9360
Affinity Capture-MS Homo sapiens
136 SON 6651
Affinity Capture-MS Homo sapiens
137 SAP18 10284
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
138 TPT1 7178
Two-hybrid Homo sapiens
139 SNRPA 6626
Affinity Capture-MS Homo sapiens
140 PRPF19 27339
Affinity Capture-MS Homo sapiens
141 CAPZA2 830
Affinity Capture-MS Homo sapiens
Two-hybrid Homo sapiens
142 THRAP3 9967
Affinity Capture-MS Homo sapiens
143 SP7  
Proximity Label-MS Homo sapiens
144 Kifc5b  
Two-hybrid Mus musculus
145 CHD4 1108
Affinity Capture-MS Homo sapiens
146 EIF4A3 9775
Affinity Capture-MS Homo sapiens
147 EZH1  
Affinity Capture-MS Homo sapiens
148 NIFK 84365
Affinity Capture-MS Homo sapiens
149 NDUFB9 4715
Two-hybrid Homo sapiens
150 ESR1  
Affinity Capture-Western Homo sapiens
151 DHX40  
Proximity Label-MS Homo sapiens
152 EVA1C  
Two-hybrid Homo sapiens
153 NUP50 10762
Proximity Label-MS Homo sapiens
154 TRIM37  
Proximity Label-MS Homo sapiens
155 GLUL 2752
Two-hybrid Homo sapiens
156 SRSF7 6432
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Two-hybrid Homo sapiens
157 ZCCHC10  
Affinity Capture-MS Homo sapiens
158 CIT 11113
Affinity Capture-MS Homo sapiens
159 KRAS 3845
Synthetic Lethality Homo sapiens
160 Ufl1  
Affinity Capture-MS Mus musculus
161 FBXO7 25793
Two-hybrid Homo sapiens
162 PCNA 5111
Affinity Capture-MS Homo sapiens
163 RBM34  
Co-fractionation Homo sapiens
164 Prkaa1  
Affinity Capture-MS Mus musculus
165 SSRP1 6749
Affinity Capture-MS Homo sapiens
166 MEN1 4221
Affinity Capture-MS Homo sapiens
167 Uso1 56041
Affinity Capture-MS Mus musculus
168 LUC7L 55692
Affinity Capture-MS Homo sapiens
169 TRPC4AP  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
170 EP300 2033
Affinity Capture-MS Homo sapiens
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