Gene description for MAPKAPK2
Gene name mitogen-activated protein kinase-activated protein kinase 2
Gene symbol MAPKAPK2
Other names/aliases MAPKAP-K2
MK-2
MK2
Species Homo sapiens
 Database cross references - MAPKAPK2
ExoCarta ExoCarta_9261
Vesiclepedia VP_9261
Entrez Gene 9261
HGNC 6887
MIM 602006
UniProt P49137  
 MAPKAPK2 identified in exosomes derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Mesenchymal stem cells 36408942    
Thymus 23844026    
 Gene ontology annotations for MAPKAPK2
Molecular Function
    calmodulin-dependent protein kinase activity GO:0004683 IBA
    calmodulin binding GO:0005516 IBA
    protein serine/threonine kinase activity GO:0004674 IDA
    ATP binding GO:0005524 IEA
    mitogen-activated protein kinase binding GO:0051019 IBA
    calcium-dependent protein serine/threonine kinase activity GO:0009931 IBA
    protein binding GO:0005515 IPI
    signal transducer activity GO:0004871 TAS
    protein kinase activity GO:0004672 TAS
Biological Process
    toll-like receptor 9 signaling pathway GO:0034162 TAS
    G2 DNA damage checkpoint GO:0031572 IMP
    toll-like receptor TLR6:TLR2 signaling pathway GO:0038124 TAS
    vascular endothelial growth factor receptor signaling pathway GO:0048010 TAS
    TRIF-dependent toll-like receptor signaling pathway GO:0035666 TAS
    Ras protein signal transduction GO:0007265 TAS
    toll-like receptor signaling pathway GO:0002224 TAS
    inflammatory response GO:0006954 ISS
    peptidyl-serine phosphorylation GO:0018105 IDA
    protein autophosphorylation GO:0046777 IBA
    gene expression GO:0010467 TAS
    MAPK cascade GO:0000165 TAS
    regulation of interleukin-6 production GO:0032675 ISS
    neurotrophin TRK receptor signaling pathway GO:0048011 TAS
    stress-activated MAPK cascade GO:0051403 TAS
    innate immune response GO:0045087 TAS
    regulation of tumor necrosis factor production GO:0032680 IDA
    MyD88-dependent toll-like receptor signaling pathway GO:0002755 TAS
    cellular response to heat GO:0034605 TAS
    response to lipopolysaccharide GO:0032496 ISS
    toll-like receptor 2 signaling pathway GO:0034134 TAS
    toll-like receptor 10 signaling pathway GO:0034166 TAS
    3'-UTR-mediated mRNA stabilization GO:0070935 IDA
    activation of MAPK activity GO:0000187 TAS
    toll-like receptor 5 signaling pathway GO:0034146 TAS
    MyD88-independent toll-like receptor signaling pathway GO:0002756 TAS
    toll-like receptor TLR1:TLR2 signaling pathway GO:0038123 TAS
    toll-like receptor 3 signaling pathway GO:0034138 TAS
    inner ear development GO:0048839 IEA
    macropinocytosis GO:0044351 ISS
    protein phosphorylation GO:0006468 TAS
    response to cytokine GO:0034097 IDA
    arachidonic acid metabolic process GO:0019369 TAS
    leukotriene metabolic process GO:0006691 TAS
    small molecule metabolic process GO:0044281 TAS
    cellular response to DNA damage stimulus GO:0006974 IMP
    cellular response to vascular endothelial growth factor stimulus GO:0035924 IMP
    toll-like receptor 4 signaling pathway GO:0034142 TAS
    regulation of cellular response to heat GO:1900034 TAS
    response to stress GO:0006950 IDA
Subcellular Localization
    centrosome GO:0005813 IDA
    extracellular exosome GO:0070062 IDA
    nucleoplasm GO:0005654 TAS
    cytosol GO:0005829 TAS
    cytoplasm GO:0005737 IDA
    nucleus GO:0005634 IDA
 Experiment description of studies that identified MAPKAPK2 in exosomes
1
Experiment ID 489
MISEV standards
EM
EV Biophysical techniques
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
EV Enriched markers
Canx
EV Negative markers
NTA
EV Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ.
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
EM
EV Biophysical techniques
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
EV Enriched markers
Canx
EV Negative markers
NTA
EV Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ.
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
EM
EV Biophysical techniques
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
EV Enriched markers
Canx
EV Negative markers
NTA
EV Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ.
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
EM
EV Biophysical techniques
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
EV Enriched markers
Canx
EV Negative markers
NTA
EV Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ.
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
5
Experiment ID 488
MISEV standards
EM
EV Biophysical techniques
CD9|CD81|CD63|GAPDH|SDCBP|LAMP1|TFRC|UCHL1|FLOT2|LAMP2|FLOT1|ICAM1|RAB5B|CD151|RAB35|TSG101|RAB5A|CD82
EV Enriched markers
CANX
EV Negative markers
NTA
EV Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Homo sapiens
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ.
Journal name Proteomics
Publication year 2023
Sample Mesenchymal stem cells
Sample name UCMSC
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
6
Experiment ID 217
MISEV standards
EM
EV Biophysical techniques
TSG101|CD81|CD9|CD63
EV Enriched markers
EV Negative markers
NTA
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23844026    
Organism Homo sapiens
Experiment description Characterization of human thymic exosomes.
Authors Skogberg G, Gudmundsdottir J, van der Post S, Sandstrom K, Bruhn S, Benson M, Mincheva-Nilsson L, Baranov V, Telemo E, Ekwall O.
Journal name PLoS One
Publication year 2013
Sample Thymus
Sample name Normal-Thymus
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for MAPKAPK2
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 MAPK1 5594
Affinity Capture-Western Homo sapiens
2 EGF 1950
Phenotypic Enhancement Homo sapiens
3 PHC2  
Affinity Capture-Western Homo sapiens
Two-hybrid Homo sapiens
4 MAPK14 1432
Invivo Homo sapiens
Affinity Capture-Western Homo sapiens
5 AKT1 207
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
6 Tsc2 24855
Biochemical Activity Rattus norvegicus
7 HSPB1 3315
Affinity Capture-Western Homo sapiens
8 SHC1 6464
Affinity Capture-Western Homo sapiens
Two-hybrid Homo sapiens
Biochemical Activity Homo sapiens
9 TCF3  
Invitro Homo sapiens
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