Gene description for MAPK3
Gene name mitogen-activated protein kinase 3
Gene symbol MAPK3
Other names/aliases ERK-1
ERK1
ERT2
HS44KDAP
HUMKER1A
P44ERK1
P44MAPK
PRKM3
p44-ERK1
p44-MAPK
Species Homo sapiens
 Database cross references - MAPK3
ExoCarta ExoCarta_5595
Entrez Gene 5595
HGNC 6877
MIM 601795
UniProt P27361  
 MAPK3 identified in exosomes derived from the following tissue/cell type
Keratinocytes 19530224    
Keratinocytes 19530224    
Neuroblastoma cells 25944692    
Ovarian cancer cells 23333927    
Ovarian cancer cells 23333927    
Squamous carcinoma cells 20124223    
Thymus 23844026    
 Gene ontology annotations for MAPK3
Molecular Function
    ATP binding GO:0005524 IEA
    phosphatase binding GO:0019902 IPI
    protein binding GO:0005515 IPI
    phosphotyrosine binding GO:0001784 IEA
    MAP kinase activity GO:0004707 TAS
Biological Process
    toll-like receptor 3 signaling pathway GO:0034138 TAS
    insulin receptor signaling pathway GO:0008286 TAS
    phosphorylation GO:0016310 IDA
    toll-like receptor 9 signaling pathway GO:0034162 TAS
    stress-activated MAPK cascade GO:0051403 TAS
    activation of MAPKK activity GO:0000186 TAS
    epidermal growth factor receptor signaling pathway GO:0007173 TAS
    response to epidermal growth factor GO:0070849 IDA
    Fc-gamma receptor signaling pathway involved in phagocytosis GO:0038096 TAS
    regulation of stress-activated MAPK cascade GO:0032872 TAS
    fibroblast growth factor receptor signaling pathway GO:0008543 TAS
    activation of MAPK activity GO:0000187 TAS
    positive regulation of protein phosphorylation GO:0001934 IMP
    Ras protein signal transduction GO:0007265 TAS
    Fc-epsilon receptor signaling pathway GO:0038095 TAS
    cellular response to mechanical stimulus GO:0071260 IEP
    cytokine-mediated signaling pathway GO:0019221 TAS
    blood coagulation GO:0007596 TAS
    cellular response to heat GO:0034605 TAS
    BMP signaling pathway GO:0030509 IMP
    negative regulation of apolipoprotein binding GO:2000657 IEA
    transcription from RNA polymerase I promoter GO:0006360 TAS
    apoptotic process GO:0006915 IEA
    viral process GO:0016032 IEA
    axon guidance GO:0007411 TAS
    regulation of cytoskeleton organization GO:0051493 TAS
    positive regulation of histone phosphorylation GO:0033129 IMP
    neurotrophin TRK receptor signaling pathway GO:0048011 TAS
    caveolin-mediated endocytosis GO:0072584 TAS
    transcription initiation from RNA polymerase I promoter GO:0006361 TAS
    toll-like receptor TLR6:TLR2 signaling pathway GO:0038124 TAS
    lipopolysaccharide-mediated signaling pathway GO:0031663 IEA
    positive regulation of ERK1 and ERK2 cascade GO:0070374 IMP
    regulation of sequence-specific DNA binding transcription factor activity GO:0051090 TAS
    innate immune response GO:0045087 TAS
    JAK-STAT cascade involved in growth hormone signaling pathway GO:0060397 TAS
    gene expression GO:0010467 TAS
    positive regulation of cyclase activity GO:0031281 IMP
    positive regulation of histone acetylation GO:0035066 IMP
    vascular endothelial growth factor receptor signaling pathway GO:0048010 TAS
    MyD88-dependent toll-like receptor signaling pathway GO:0002755 TAS
    DNA damage induced protein phosphorylation GO:0006975 IDA
    regulation of Golgi inheritance GO:0090170 TAS
    platelet activation GO:0030168 TAS
    sensory perception of pain GO:0019233 IEA
    cartilage development GO:0051216 IEA
    small GTPase mediated signal transduction GO:0007264 TAS
    interleukin-1-mediated signaling pathway GO:0070498 IMP
    MyD88-independent toll-like receptor signaling pathway GO:0002756 TAS
    MAPK cascade GO:0000165 TAS
    regulation of cellular response to heat GO:1900034 TAS
    toll-like receptor 5 signaling pathway GO:0034146 TAS
    cell cycle GO:0007049 IEA
    regulation of early endosome to late endosome transport GO:2000641 TAS
    toll-like receptor 2 signaling pathway GO:0034134 TAS
    peptidyl-tyrosine autophosphorylation GO:0038083 IDA
    toll-like receptor 10 signaling pathway GO:0034166 TAS
    toll-like receptor 4 signaling pathway GO:0034142 TAS
    toll-like receptor TLR1:TLR2 signaling pathway GO:0038123 TAS
    positive regulation of transcription from RNA polymerase II promoter GO:0045944 IMP
    response to exogenous dsRNA GO:0043330 IEA
    toll-like receptor signaling pathway GO:0002224 TAS
    organ morphogenesis GO:0009887 IEA
    TRIF-dependent toll-like receptor signaling pathway GO:0035666 TAS
    protein phosphorylation GO:0006468 IDA
Subcellular Localization
    extracellular exosome GO:0070062 IDA
    nucleus GO:0005634 TAS
    cytoskeleton GO:0005856 TAS
    late endosome GO:0005770 TAS
    caveola GO:0005901 TAS
    focal adhesion GO:0005925 TAS
    nucleoplasm GO:0005654 TAS
    pseudopodium GO:0031143 IEA
    early endosome GO:0005769 TAS
    Golgi apparatus GO:0005794 TAS
    mitochondrion GO:0005739 TAS
    nuclear envelope GO:0005635 IDA
    cytosol GO:0005829 TAS
    microtubule cytoskeleton GO:0015630 IDA
 Experiment description of studies that identified MAPK3 in exosomes
1
Experiment ID 189
ISEV standards
EM
EV Biophysical techniques
EV Cytosolic markers
EV Membrane markers
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 19530224    
Organism Homo sapiens
Experiment description Profile of exosomes related proteins released by differentiated and undifferentiated human keratinocytes.
Authors Chavez-Muñoz C, Kilani RT, Ghahary A.
Journal name J Cell Physiol
Publication year 2009
Sample Keratinocytes
Sample name Keratinocytes - Differentiated
Isolation/purification methods Differential centrifugation
Filtration
Sucrose cushion
Filtration
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
2
Experiment ID 190
ISEV standards
EM
EV Biophysical techniques
HSC70
EV Cytosolic markers
EV Membrane markers
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 19530224    
Organism Homo sapiens
Experiment description Profile of exosomes related proteins released by differentiated and undifferentiated human keratinocytes.
Authors Chavez-Muñoz C, Kilani RT, Ghahary A.
Journal name J Cell Physiol
Publication year 2009
Sample Keratinocytes
Sample name Keratinocytes - Undifferentiated
Isolation/purification methods Differential centrifugation
Filtration
Sucrose cushion
Filtration
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
3
Experiment ID 224
ISEV standards
EM|AFM
EV Biophysical techniques
Alix|TSG101
EV Cytosolic markers
CD63|CD81
EV Membrane markers
GOLGA2
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25944692    
Organism Homo sapiens
Experiment description Proteogenomic analysis reveals exosomes are more oncogenic than ectosomes
Authors Keerthikumar S, Gangoda L, Liem M, Fonseka P, Atukorala I, Ozcitti C, Mechler A, Adda CG, Ang CS, Mathivanan S
Journal name Oncotarget
Publication year 2015
Sample Neuroblastoma cells
Sample name SH-SY5Y
Isolation/purification methods Differential centrifugation
Ultracentrifugation
OptiPrep density gradient
Flotation density 1.10 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
Western blotting
4
Experiment ID 211
ISEV standards
EM
EV Biophysical techniques
TSG101|Alix
EV Cytosolic markers
EpCAM|TFRC
EV Membrane markers
cytochrome c|GOLGA2
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23333927    
Organism Homo sapiens
Experiment description Characterization and proteomic analysis of ovarian cancer-derived exosomes.
Authors Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K.
Journal name J Proteomics
Publication year 2013
Sample Ovarian cancer cells
Sample name IGROV1
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.09-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
5
Experiment ID 212
ISEV standards
CEM
EV Biophysical techniques
TSG101|Alix
EV Cytosolic markers
EpCAM|TFRC
EV Membrane markers
Cytochrome C|GOLGA2
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23333927    
Organism Homo sapiens
Experiment description Characterization and proteomic analysis of ovarian cancer-derived exosomes.
Authors Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K.
Journal name J Proteomics
Publication year 2013
Sample Ovarian cancer cells
Sample name OVCAR-3
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.09-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
6
Experiment ID 191
ISEV standards
EV Biophysical techniques
Alix
EV Cytosolic markers
CD81|CD9
EV Membrane markers
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 20124223    
Organism Homo sapiens
Experiment description Hypoxic tumor cell modulates its microenvironment to enhance angiogenic and metastatic potential by secretion of proteins and exosomes.
Authors Park JE, Tan HS, Datta A, Lai RC, Zhang H, Meng W, Lim SK, Sze SK.
Journal name Mol Cell Proteomics
Publication year 2010
Sample Squamous carcinoma cells
Sample name Squamous carcinoma cell (A431)
Isolation/purification methods Differential centrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
7
Experiment ID 217
ISEV standards
EM
EV Biophysical techniques
TSG101
EV Cytosolic markers
CD81|CD9|CD63
EV Membrane markers
EV Negative markers
NTA
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23844026    
Organism Homo sapiens
Experiment description Characterization of human thymic exosomes.
Authors Skogberg G, Gudmundsdottir J, van der Post S, Sandstrom K, Bruhn S, Benson M, Mincheva-Nilsson L, Baranov V, Telemo E, Ekwall O.
Journal name PLoS One
Publication year 2013
Sample Thymus
Sample name Normal-Thymus
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for MAPK3
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 SNCA 6622
Affinity Capture-Western Homo sapiens
2 MAP2K2 5605
Invitro Homo sapiens
Two-hybrid Homo sapiens
Biochemical Activity Homo sapiens
3 HDAC4  
Invitro Homo sapiens
4 DUSP1  
Invivo Homo sapiens
Invitro Homo sapiens
5 SPIB  
Co-purification Homo sapiens
Biochemical Activity Homo sapiens
6 DUSP5  
Invitro Homo sapiens
7 Rps6kb1  
Reconstituted Complex Mus musculus
8 EDD  
Affinity Capture-Western Homo sapiens
9 TH  
Invivo Homo sapiens
10 GMFB  
Invivo Homo sapiens
11 GTF2I 2969
Invivo Homo sapiens
Invitro Homo sapiens
12 DUSP3 1845
Invivo Homo sapiens
Invitro Homo sapiens
13 SNCG 6623
Affinity Capture-Western Homo sapiens
14 JUN 3725
Biochemical Activity Homo sapiens
15 INSR 3643
Affinity Capture-Western Homo sapiens
16 RPS6KA4 8986
Affinity Capture-Western Homo sapiens
17 Rps6ka3  
Affinity Capture-Western Mus musculus
18 IER3  
Affinity Capture-Western Homo sapiens
19 RPS6KA2  
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
20 ARRB2  
Affinity Capture-Western Homo sapiens
21 MAP2K1 5604
Two-hybrid Homo sapiens
Biochemical Activity Homo sapiens
Invivo Homo sapiens
Invivo Homo sapiens
Invitro Homo sapiens
Invitro Homo sapiens
Affinity Capture-Western Homo sapiens
22 LYN 4067
Affinity Capture-Western Homo sapiens
23 DUSP6 1848
Invitro Homo sapiens
24 HIF1A  
Invivo Homo sapiens
Invitro Homo sapiens
25 PTPN7 5778
Biochemical Activity Homo sapiens
Reconstituted Complex Homo sapiens
Invivo Homo sapiens
Invitro Homo sapiens
26 ELK1  
Invivo Homo sapiens
27 PTPN5  
Reconstituted Complex Homo sapiens
28 RPS6KA3 6197
Affinity Capture-Western Homo sapiens
29 MAP2K3 5606
Invitro Homo sapiens
30 MYC  
Reconstituted Complex Homo sapiens
31 MYLK 4638
Invitro Homo sapiens
32 ATP1A1 476
Biochemical Activity Homo sapiens
33 TP53  
Invivo Homo sapiens
34 DCC  
Two-hybrid Homo sapiens
35 C1QBP 708
Invitro Homo sapiens
Invivo Homo sapiens
36 RAF1 5894
Affinity Capture-Western Homo sapiens
37 BRAF  
Phenotypic Enhancement Homo sapiens
38 MAPK14 1432
Affinity Capture-Western Homo sapiens
39 SREBF2 6721
Invitro Homo sapiens
40 PTPRR  
Reconstituted Complex Homo sapiens
41 RPS6KA1 6195
Affinity Capture-Western Homo sapiens
42 LAMTOR3 8649
Affinity Capture-Western Homo sapiens
43 PEA15  
Invivo Homo sapiens
Invitro Homo sapiens
Two-hybrid Homo sapiens
44 AVPR2  
Affinity Capture-Western Homo sapiens
View the network image/svg+xml



Perform bioinformatics analysis of your extracellular vesicle data set using FunRich, a open access standalone tool. NEW UPDATED VERSION OF FunRich available for download (12/09/2016) from here