Gene description for TIAL1
Gene name TIA1 cytotoxic granule-associated RNA binding protein-like 1
Gene symbol TIAL1
Other names/aliases TCBP
TIAR
Species Homo sapiens
 Database cross references - TIAL1
ExoCarta ExoCarta_7073
Vesiclepedia VP_7073
Entrez Gene 7073
HGNC 11804
MIM 603413
UniProt Q01085  
 TIAL1 identified in exosomes derived from the following tissue/cell type
Gastric cancer cells 33991177    
Gastric cancer cells 33991177    
Gastric cancer cells 33991177    
Thymus 23844026    
 Gene ontology annotations for TIAL1
Molecular Function
    DNA binding GO:0003677 IEA
    RNA binding GO:0003723 HDA
    mRNA 3'-UTR binding GO:0003730 IDA
    protein binding GO:0005515 IPI
    mRNA 3'-UTR AU-rich region binding GO:0035925 IEA
    protein-RNA adaptor activity GO:0140517 IDA
Biological Process
    regulation of transcription by RNA polymerase II GO:0006357 TAS
    apoptotic process GO:0006915 IEA
    defense response GO:0006952 TAS
    germ cell development GO:0007281 IEA
    negative regulation of cell population proliferation GO:0008285 IMP
    stem cell division GO:0017145 IEA
    positive regulation of hippo signaling GO:0035332 IMP
    stem cell proliferation GO:0072089 IEA
    positive regulation of miRNA-mediated gene silencing GO:2000637 IGI
    positive regulation of stem cell proliferation GO:2000648 IEA
Subcellular Localization
    nucleus GO:0005634 ISS
    nucleoplasm GO:0005654 IDA
    nucleoplasm GO:0005654 TAS
    cytoplasm GO:0005737 ISS
    lysosome GO:0005764 TAS
    cytosol GO:0005829 IDA
    cytoplasmic stress granule GO:0010494 ISS
    cytolytic granule GO:0044194 IEA
 Experiment description of studies that identified TIAL1 in exosomes
1
Experiment ID 363
MISEV standards
✘ Biophysical techniques
✔
TSG101|GAPDH|AQP1|CD151|CD81|CD82|CD9|EPCAM|FLOT1|FLOT2|ICAM1|ITGA2B|LAMP2|RAB35|RAB5A|RAB5B|SDCBP|TFRC|UCHL1
Enriched markers
✔
DCLK1
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 33991177    
Organism Homo sapiens
Experiment description Cancer stem cell marker DCLK1 reprograms small extracellular vesicles toward migratory phenotype in gastric cancer cells
Authors "Carli ALE, Afshar-Sterle S, Rai A, Fang H, O'Keefe R, Tse J, Ferguson FM, Gray NS, Ernst M, Greening DW, Buchert M."
Journal name Proteomics
Publication year 2021
Sample Gastric cancer cells
Sample name MKN1 - 100K pellet
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
2
Experiment ID 364
MISEV standards
✘ Biophysical techniques
✔
TSG101|GAPDH|AQP1|CD151|CD81|CD82|CD9|EPCAM|FLOT1|FLOT2|ICAM1|ITGA2B|LAMP2|RAB35|RAB5A|RAB5B|SDCBP|TFRC|UCHL1
Enriched markers
✔
DCLK1
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 33991177    
Organism Homo sapiens
Experiment description Cancer stem cell marker DCLK1 reprograms small extracellular vesicles toward migratory phenotype in gastric cancer cells
Authors "Carli ALE, Afshar-Sterle S, Rai A, Fang H, O'Keefe R, Tse J, Ferguson FM, Gray NS, Ernst M, Greening DW, Buchert M."
Journal name Proteomics
Publication year 2021
Sample Gastric cancer cells
Sample name MKN1 - 100K pellet
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
3
Experiment ID 365
MISEV standards
✘ Biophysical techniques
✔
TSG101|GAPDH|AQP1|CD151|CD81|CD82|CD9|EPCAM|FLOT1|FLOT2|ICAM1|ITGA2B|LAMP2|RAB35|RAB5A|RAB5B|SDCBP|TFRC|UCHL1
Enriched markers
✔
DCLK1
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 33991177    
Organism Homo sapiens
Experiment description Cancer stem cell marker DCLK1 reprograms small extracellular vesicles toward migratory phenotype in gastric cancer cells
Authors "Carli ALE, Afshar-Sterle S, Rai A, Fang H, O'Keefe R, Tse J, Ferguson FM, Gray NS, Ernst M, Greening DW, Buchert M."
Journal name Proteomics
Publication year 2021
Sample Gastric cancer cells
Sample name MKN1 - 100K pellet
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
4
Experiment ID 217
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|CD81|CD9|CD63
Enriched markers
✘ Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23844026    
Organism Homo sapiens
Experiment description Characterization of human thymic exosomes.
Authors "Skogberg G, Gudmundsdottir J, van der Post S, Sandstrom K, Bruhn S, Benson M, Mincheva-Nilsson L, Baranov V, Telemo E, Ekwall O."
Journal name PLoS One
Publication year 2013
Sample Thymus
Sample name Normal-Thymus
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for TIAL1
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 BPIFA1 51297
Two-hybrid Homo sapiens
2 PRR13  
Two-hybrid Homo sapiens
3 RPA2 6118
Affinity Capture-MS Homo sapiens
Proximity Label-MS Homo sapiens
4 QKI 9444
Two-hybrid Homo sapiens
5 CAND1 55832
Affinity Capture-MS Homo sapiens
6 PIR 8544
Co-fractionation Homo sapiens
7 SBDS 51119
Co-fractionation Homo sapiens
8 APEX1 328
Affinity Capture-RNA Homo sapiens
9 INA 9118
Affinity Capture-MS Homo sapiens
10 PTRHD1 391356
Co-fractionation Homo sapiens
11 DAZAP1 26528
Co-fractionation Homo sapiens
12 COPS5 10987
Affinity Capture-MS Homo sapiens
13 POU5F1  
Affinity Capture-MS Homo sapiens
14 TRO  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
15 OBSL1 23363
Affinity Capture-MS Homo sapiens
16 CUL4A 8451
Affinity Capture-MS Homo sapiens
17 KRT31 3881
Two-hybrid Homo sapiens
18 SRI 6717
Co-fractionation Homo sapiens
19 SARNP 84324
Co-fractionation Homo sapiens
20 VCAM1 7412
Affinity Capture-MS Homo sapiens
21 TOMM40 10452
Co-fractionation Homo sapiens
22 CUL1 8454
Affinity Capture-MS Homo sapiens
23 MYCN  
Affinity Capture-MS Homo sapiens
24 NEDD8 4738
Affinity Capture-MS Homo sapiens
25 SOD1 6647
Co-fractionation Homo sapiens
26 PRR20A  
Two-hybrid Homo sapiens
27 EMC9  
Affinity Capture-MS Homo sapiens
28 MRPS25 64432
Cross-Linking-MS (XL-MS) Homo sapiens
29 MAPK3 5595
Affinity Capture-MS Homo sapiens
30 ALDH5A1 7915
Co-fractionation Homo sapiens
31 CUL4B 8450
Affinity Capture-MS Homo sapiens
32 DUT 1854
Co-fractionation Homo sapiens
33 KRTAP8-1  
Two-hybrid Homo sapiens
34 DAZAP2  
Two-hybrid Homo sapiens
35 CELF4  
Two-hybrid Homo sapiens
36 FUBP1 8880
Two-hybrid Homo sapiens
37 C10orf55  
Two-hybrid Homo sapiens
38 ITGA4 3676
Affinity Capture-MS Homo sapiens
39 CUL2 8453
Affinity Capture-MS Homo sapiens
40 MBNL2 10150
Two-hybrid Homo sapiens
41 SND1 27044
Cross-Linking-MS (XL-MS) Homo sapiens
42 CEBPA  
Protein-peptide Homo sapiens
43 PCBP2 5094
Co-fractionation Homo sapiens
44 CUL3 8452
Affinity Capture-MS Homo sapiens
45 PITRM1 10531
Affinity Capture-MS Homo sapiens
46 XPO1 7514
Affinity Capture-MS Homo sapiens
47 RSU1 6251
Co-fractionation Homo sapiens
48 KLF1  
Two-hybrid Homo sapiens
49 PRR20D  
Two-hybrid Homo sapiens
50 KBTBD7  
Affinity Capture-MS Homo sapiens
51 FN1 2335
Affinity Capture-MS Homo sapiens
52 MRPS28  
Co-fractionation Homo sapiens
53 UBE2D2 7322
Co-fractionation Homo sapiens
54 PRR20B  
Two-hybrid Homo sapiens
55 PCBP1 5093
Co-fractionation Homo sapiens
56 DCUN1D1 54165
Affinity Capture-MS Homo sapiens
57 MYC  
Affinity Capture-MS Homo sapiens
58 RPA3 6119
Affinity Capture-MS Homo sapiens
Proximity Label-MS Homo sapiens
59 SAV1  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
60 PCGF1 84759
Affinity Capture-MS Homo sapiens
61 BMI1  
Affinity Capture-MS Homo sapiens
62 VDAC1 7416
Co-fractionation Homo sapiens
63 RNF26  
Affinity Capture-MS Homo sapiens
64 NANOG  
Affinity Capture-MS Homo sapiens
65 THY1 7070
Co-fractionation Homo sapiens
66 GNB2L1 10399
Co-fractionation Homo sapiens
67 PRR20C  
Two-hybrid Homo sapiens
68 MAPK1IP1L 93487
Two-hybrid Homo sapiens
69 DZIP3  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
70 KIF2A 3796
Affinity Capture-MS Homo sapiens
71 CCDC58 131076
Co-fractionation Homo sapiens
72 PLEKHA4 57664
Affinity Capture-MS Homo sapiens
73 VPS37C 55048
Two-hybrid Homo sapiens
74 RAB10 10890
Co-fractionation Homo sapiens
75 SF3B4 10262
Co-fractionation Homo sapiens
76 MRPL53  
Co-fractionation Homo sapiens
77 INCA1  
Two-hybrid Homo sapiens
78 ACAA2 10449
Co-fractionation Homo sapiens
79 SNRPA 6626
Affinity Capture-MS Homo sapiens
80 CREBBP  
Two-hybrid Homo sapiens
81 FAM83H 286077
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
82 DDX58 23586
Affinity Capture-RNA Homo sapiens
83 SSBP1 6742
Co-fractionation Homo sapiens
84 COPS6 10980
Affinity Capture-MS Homo sapiens
85 Ppp6r1  
Affinity Capture-MS Mus musculus
86 MBNL1 4154
Co-fractionation Homo sapiens
87 MAGED1 9500
Two-hybrid Homo sapiens
88 PRR20E  
Two-hybrid Homo sapiens
89 TRIM31  
Affinity Capture-MS Homo sapiens
90 RBPMS2  
Two-hybrid Homo sapiens
91 CIT 11113
Affinity Capture-MS Homo sapiens
92 CUL7 9820
Affinity Capture-MS Homo sapiens
93 C9orf72  
Affinity Capture-MS Homo sapiens
94 RPA1 6117
Affinity Capture-MS Homo sapiens
95 GRINA  
Two-hybrid Homo sapiens
96 SCAMP3 10067
Co-fractionation Homo sapiens
97 FAM168B 130074
Two-hybrid Homo sapiens
98 UFM1 51569
Co-fractionation Homo sapiens
99 SCO2  
Co-fractionation Homo sapiens
100 CUL5 8065
Affinity Capture-MS Homo sapiens
101 COTL1 23406
Co-fractionation Homo sapiens
View the network image/svg+xml



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