Gene description for MAGED1
Gene name melanoma antigen family D1
Gene symbol MAGED1
Other names/aliases DLXIN-1
NRAGE
Species Homo sapiens
 Database cross references - MAGED1
ExoCarta ExoCarta_9500
Vesiclepedia VP_9500
Entrez Gene 9500
HGNC 6813
MIM 300224
UniProt Q9Y5V3  
 MAGED1 identified in exosomes derived from the following tissue/cell type
Mesenchymal stem cells 36408942    
 Gene ontology annotations for MAGED1
Molecular Function
    transcription coactivator activity GO:0003713 IEA
    protein binding GO:0005515 IPI
Biological Process
    negative regulation of transcription, DNA-templated GO:0045892 IEA
    regulation of apoptotic process GO:0042981 TAS
    positive regulation of apoptotic process GO:0043065 TAS
    positive regulation of branching involved in ureteric bud morphogenesis GO:0090190 IEA
    negative regulation of epithelial cell proliferation GO:0050680 IDA
    regulation of circadian rhythm GO:0042752 IEA
    neurotrophin TRK receptor signaling pathway GO:0048011 TAS
    positive regulation of apoptotic signaling pathway GO:2001235 IEA
    positive regulation of transcription, DNA-templated GO:0045893 IEA
    extrinsic apoptotic signaling pathway GO:0097191 TAS
    positive regulation of MAP kinase activity GO:0043406 IEA
    regulation of transcription, DNA-templated GO:0006355 TAS
    apoptotic process GO:0006915 TAS
    regulation of transcription from RNA polymerase II promoter GO:0006357 IEA
    extrinsic apoptotic signaling pathway in absence of ligand GO:0097192 TAS
    programmed cell death GO:0012501 TAS
    circadian regulation of gene expression GO:0032922 ISS
    apoptotic signaling pathway GO:0097190 TAS
Subcellular Localization
    chromatin GO:0000785 ISS
    protein complex GO:0043234 IDA
    nucleus GO:0005634 ISS
    plasma membrane GO:0005886 IEA
    cytoplasm GO:0005737 IEA
 Experiment description of studies that identified MAGED1 in exosomes
1
Experiment ID 488
MISEV standards
✔
EM
EV Biophysical techniques
✔
CD9|CD81|CD63|GAPDH|SDCBP|LAMP1|TFRC|UCHL1|FLOT2|LAMP2|FLOT1|ICAM1|RAB5B|CD151|RAB35|TSG101|RAB5A|CD82
EV Enriched markers
✔
CANX
EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Homo sapiens
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ.
Journal name Proteomics
Publication year 2023
Sample Mesenchymal stem cells
Sample name UCMSC
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
 Protein-protein interactions for MAGED1
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 COPG2 26958
Affinity Capture-MS Homo sapiens
2 SMC3 9126
Affinity Capture-MS Homo sapiens
3 TIMM50 92609
Affinity Capture-MS Homo sapiens
4 NUP160 23279
Affinity Capture-MS Homo sapiens
5 XIAP  
Invitro Homo sapiens
Invivo Homo sapiens
Two-hybrid Homo sapiens
6 NUP205 23165
Affinity Capture-MS Homo sapiens
7 MPC1 51660
Two-hybrid Homo sapiens
8 DLX5  
Invivo Homo sapiens
Two-hybrid Homo sapiens
9 SIRT7  
Two-hybrid Homo sapiens
10 DLX4  
Invivo Homo sapiens
11 SPTLC1 10558
Affinity Capture-MS Homo sapiens
12 DNAAF5 54919
Affinity Capture-MS Homo sapiens
13 SLC25A1 6576
Affinity Capture-MS Homo sapiens
14 FANCI 55215
Affinity Capture-MS Homo sapiens
15 RFX1  
Two-hybrid Homo sapiens
16 PJA2  
Two-hybrid Homo sapiens
17 ATXN10 25814
Affinity Capture-MS Homo sapiens
18 CCAR2 57805
Affinity Capture-MS Homo sapiens
19 MSX2  
Invivo Homo sapiens
Invivo Homo sapiens
20 GCN1L1 10985
Affinity Capture-MS Homo sapiens
21 AKAP8L  
Affinity Capture-MS Homo sapiens
22 ROR2 4920
Affinity Capture-Western Homo sapiens
23 BAG6 7917
Affinity Capture-MS Homo sapiens
24 LRPPRC 10128
Affinity Capture-MS Homo sapiens
25 Tuba1a 22142
Two-hybrid Mus musculus
26 NOC2L 26155
Affinity Capture-MS Homo sapiens
27 UBR5 51366
Affinity Capture-MS Homo sapiens
28 MAGED1 9500
Invivo Homo sapiens
Invivo Homo sapiens
29 EIF3J 8669
Two-hybrid Homo sapiens
30 CALML5 51806
Affinity Capture-MS Homo sapiens
31 PJA1  
Invivo Homo sapiens
Invitro Homo sapiens
Two-hybrid Homo sapiens
32 PLK1 5347
Two-hybrid Homo sapiens
33 TELO2 9894
Affinity Capture-MS Homo sapiens
34 GPR135  
Two-hybrid Homo sapiens
35 HSPC128  
Affinity Capture-MS Homo sapiens
36 HSPB1 3315
Affinity Capture-MS Homo sapiens
37 IARS 3376
Affinity Capture-MS Homo sapiens
38 HEATR1 55127
Affinity Capture-MS Homo sapiens
39 COPG1 22820
Affinity Capture-MS Homo sapiens
40 EIF3E 3646
Affinity Capture-MS Homo sapiens
41 UNC5A  
Two-hybrid Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
42 NEDD8 4738
Affinity Capture-MS Homo sapiens
43 MTHFD1L 25902
Affinity Capture-MS Homo sapiens
44 NGFR 4804
Affinity Capture-Western Homo sapiens
View the network image/svg+xml
 Pathways in which MAGED1 is involved
PathwayEvidenceSource
Ligand-independent caspase activation via DCC TAS Reactome
NRAGE signals death through JNK TAS Reactome





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