ExoCarta: Gene summary

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Gene description for IRS4
Gene name	insulin receptor substrate 4
Gene symbol	IRS4
Other names/aliases	IRS-4 PY160
Species	Homo sapiens
Database cross references - IRS4
ExoCarta	ExoCarta_8471
Vesiclepedia	VP_8471
Entrez Gene	8471
HGNC	6128
MIM	300904
UniProt	O14654
IRS4 identified in sEVs derived from the following tissue/cell type
Embryonic kidney cells	36706192
Embryonic kidney cells	36706192
Neuroblastoma cells	25944692

Gene ontology annotations for IRS4

Molecular Function
	insulin receptor binding	GO:0005158	IBA
	protein binding	GO:0005515	IPI
	phosphatidylinositol 3-kinase binding	GO:0043548	IBA
Biological Process
	signal transduction	GO:0007165	TAS
	insulin receptor signaling pathway	GO:0008286	IBA
Subcellular Localization
	cytosol	GO:0005829	IBA
	plasma membrane	GO:0005886	IBA
	plasma membrane	GO:0005886	TAS

Experiment description of studies that identified IRS4 in sEVs

Experiment ID

1275

MISEV standards
	Biophysical techniques
✔ CD9\|TSG101\|FLOT1\|FLOT2\|RAB35\|RAB5A\|GAPDH\|TFRC\|CD63\|Alix	Enriched markers
✔ PCNA\|CANX\|GAPDH\|HSP90AA1\|HSP90B1	Negative markers
	Particle analysis

Identified molecule

Protein

Identification method

Mass spectrometry

PubMed ID

36706192

Organism

Homo sapiens

Experiment description

Displaying and delivering viral membrane antigens via WW domain-activated extracellular vesicles

Authors

"Choi S, Yang Z, Wang Q, Qiao Z, Sun M, Wiggins J, Xiang SH, Lu Q."

Journal name

Sci Adv

Publication year

2023

Sample

Embryonic kidney cells

Sample name

HEK293T

Isolation/purification methods

Differential centrifugation
Filtration
Ultracentrifugation

Flotation density

Molecules identified in the study

Protein

Methods used in the study

Western blotting
Mass spectrometry

Experiment ID

1280

MISEV standards
	Biophysical techniques
✔ TSG101\|CD9\|FLOT1\|FLOT2\|RAB35\|RAB5A\|GAPDH\|TFRC\|CD63\|Alix	Enriched markers
✔ PCNA\|CANX\|GAPDH\|HSP90AA1\|HSP90B1	Negative markers
	Particle analysis

Identified molecule

Protein

Identification method

Mass spectrometry

PubMed ID

36706192

Organism

Homo sapiens

Experiment description

Displaying and delivering viral membrane antigens via WW domain-activated extracellular vesicles

Authors

"Choi S, Yang Z, Wang Q, Qiao Z, Sun M, Wiggins J, Xiang SH, Lu Q."

Journal name

Sci Adv

Publication year

2023

Sample

Embryonic kidney cells

Sample name

HEK293T - Fraction 5

Isolation/purification methods

Differential centrifugation
Filtration
Ultracentrifugation
OptiPrep density gradient centrifugation

Flotation density

1.151 g/mL

Molecules identified in the study

Protein

Methods used in the study

Western blotting
Mass spectrometry

Experiment ID

224

MISEV standards
✔ EM\|AFM	Biophysical techniques
✔ Alix\|TSG101\|CD63\|CD81	Enriched markers
✔ GOLGA2	Negative markers
✘	Particle analysis

Identified molecule

protein

Identification method

Mass spectrometry

PubMed ID

25944692

Organism

Homo sapiens

Experiment description

Proteogenomic analysis reveals exosomes are more oncogenic than ectosomes

Authors

"Keerthikumar S, Gangoda L, Liem M, Fonseka P, Atukorala I, Ozcitti C, Mechler A, Adda CG, Ang CS, Mathivanan S"

Journal name

Oncotarget

Publication year

2015

Sample

Neuroblastoma cells

Sample name

SH-SY5Y

Isolation/purification methods

Differential centrifugation
Ultracentrifugation
OptiPrep density gradient

Flotation density

1.10 g/mL

Molecules identified in the study

Protein

Methods used in the study

Mass spectrometry
Western blotting

Protein-protein interactions for IRS4

Protein Interactor

ExoCarta ID

Identification method

PubMed

Species

UBE2H

7328

Affinity Capture-MS

Homo sapiens

TUBGCP3

10426

Affinity Capture-MS

Homo sapiens

GJD3

Proximity Label-MS

Homo sapiens

P4HA1

5033

Affinity Capture-Luminescence		Homo sapiens
Affinity Capture-MS		Homo sapiens

KLF8

Affinity Capture-MS

Homo sapiens

SPRTN

Affinity Capture-MS

Homo sapiens

LGR4

55366

Affinity Capture-MS

Homo sapiens

OCLN

100506658

Proximity Label-MS

Homo sapiens

MARCKS

4082

Proximity Label-MS

Homo sapiens

LAMP3

Proximity Label-MS

Homo sapiens

RIN3

Affinity Capture-MS

Homo sapiens

PARD3

56288

Proximity Label-MS

Homo sapiens

CRK

1398

Affinity Capture-Western		Homo sapiens
Reconstituted Complex		Homo sapiens

RAB35

11021

Proximity Label-MS

Homo sapiens

ACTC1

Affinity Capture-MS

Homo sapiens

FAM60A

Affinity Capture-MS

Homo sapiens

USP9X

8239

Affinity Capture-Luminescence		Homo sapiens
Affinity Capture-MS		Homo sapiens

LAMP2

3920

Proximity Label-MS

Homo sapiens

GJA1

2697

Proximity Label-MS

Homo sapiens

AKAP8L

Affinity Capture-Luminescence		Homo sapiens
Affinity Capture-MS		Homo sapiens

OTUD3

23252

Affinity Capture-MS

Homo sapiens

CDKN1A

Affinity Capture-MS

Homo sapiens

LGALS3BP

3959

Affinity Capture-MS

Homo sapiens

COPS5

10987

Affinity Capture-MS

Homo sapiens

CAPZB

832

Affinity Capture-MS

Homo sapiens

TRIM28

10155

Affinity Capture-MS

Homo sapiens

YWHAZ

7534

Affinity Capture-MS		Homo sapiens
Affinity Capture-Luminescence		Homo sapiens
Affinity Capture-MS		Homo sapiens

OTUB1

55611

Affinity Capture-MS

Homo sapiens

PCK1

5105

Affinity Capture-MS

Homo sapiens

TBL1XR1

79718

Affinity Capture-Luminescence		Homo sapiens
Affinity Capture-MS		Homo sapiens

USP18

Affinity Capture-MS		Homo sapiens
Affinity Capture-Western		Homo sapiens
Affinity Capture-Western		Homo sapiens

YWHAG

7532

Affinity Capture-MS		Homo sapiens
Affinity Capture-Luminescence		Homo sapiens
Affinity Capture-MS		Homo sapiens

KLF15

Affinity Capture-MS

Homo sapiens

PPEF1

Affinity Capture-MS

Homo sapiens

MAGED2

10916

Affinity Capture-MS		Homo sapiens
Affinity Capture-Luminescence		Homo sapiens
Affinity Capture-MS		Homo sapiens

CLPB

81570

Affinity Capture-Luminescence		Homo sapiens
Affinity Capture-MS		Homo sapiens

ALK

238

Affinity Capture-MS

Homo sapiens

RC3H1

149041

Affinity Capture-MS		Homo sapiens
Affinity Capture-MS		Homo sapiens

SLX4

Affinity Capture-MS

Homo sapiens

YWHAH

7533

Affinity Capture-Luminescence		Homo sapiens
Affinity Capture-MS		Homo sapiens

CEP57

Affinity Capture-MS

Homo sapiens

TUBA1A

7846

Affinity Capture-Luminescence		Homo sapiens
Affinity Capture-MS		Homo sapiens

CUL1

8454

Affinity Capture-MS

Homo sapiens

TFCP2

7024

Affinity Capture-MS

Homo sapiens

MYCN

Affinity Capture-MS

Homo sapiens

ERBB2

2064

Protein-peptide

Homo sapiens

EBAG9

9166

Proximity Label-MS

Homo sapiens

TUBB6

84617

Affinity Capture-Luminescence		Homo sapiens
Affinity Capture-MS		Homo sapiens

KIF14

9928

Affinity Capture-MS

Homo sapiens

YWHAB

7529

Affinity Capture-MS		Homo sapiens
Affinity Capture-MS		Homo sapiens
Affinity Capture-Luminescence		Homo sapiens
Affinity Capture-MS		Homo sapiens

MTNR1B

Reconstituted Complex

Homo sapiens

KLF16

Affinity Capture-MS

Homo sapiens

AURKA

6790

Affinity Capture-MS

Homo sapiens

NF2

4771

Affinity Capture-MS

Homo sapiens

HAUS2

Affinity Capture-MS

Homo sapiens

DLD

1738

Affinity Capture-MS

Homo sapiens

MIB1

57534

Proximity Label-MS

Homo sapiens

PPP5C

5536

Affinity Capture-MS		Homo sapiens
Proximity Label-MS		Homo sapiens
Affinity Capture-Luminescence		Homo sapiens
Affinity Capture-MS		Homo sapiens

COX15

1355

Affinity Capture-MS

Homo sapiens

EMD

2010

Affinity Capture-MS

Homo sapiens

USP11

8237

Affinity Capture-MS

Homo sapiens

C9orf78

51759

Affinity Capture-MS

Homo sapiens

MTMR11

Affinity Capture-MS

Homo sapiens

CUL4B

8450

Affinity Capture-MS

Homo sapiens

PARK2

Affinity Capture-MS

Homo sapiens

CXADR

1525

Proximity Label-MS

Homo sapiens

WDR6

11180

Affinity Capture-Luminescence		Homo sapiens
Affinity Capture-MS		Homo sapiens

DNAH17

8632

Affinity Capture-Luminescence		Homo sapiens
Affinity Capture-MS		Homo sapiens

LYN

4067

Proximity Label-MS

Homo sapiens

STAU1

6780

Affinity Capture-MS

Homo sapiens

SKAP2

8935

Affinity Capture-MS

Homo sapiens

SOCS6

Affinity Capture-MS

Homo sapiens

OTUD1

220213

Affinity Capture-MS

Homo sapiens

RB1CC1

9821

Affinity Capture-MS

Homo sapiens

YAP1

10413

Affinity Capture-MS

Homo sapiens

DIRAS3

Proximity Label-MS

Homo sapiens

VCP

7415

Affinity Capture-MS

Homo sapiens

OGT

8473

Reconstituted Complex

Homo sapiens

CRKL

1399

Reconstituted Complex

Homo sapiens

P4HA2

8974

Affinity Capture-Luminescence		Homo sapiens
Affinity Capture-MS		Homo sapiens

RLIM

51132

Affinity Capture-MS

Homo sapiens

STX6

10228

Proximity Label-MS

Homo sapiens

YWHAQ

10971

Affinity Capture-Luminescence		Homo sapiens
Affinity Capture-MS		Homo sapiens

RPA4

Proximity Label-MS

Homo sapiens

DHFRL1

Proximity Label-MS

Homo sapiens

CTSB

1508

Affinity Capture-Luminescence		Homo sapiens
Affinity Capture-MS		Homo sapiens

PIK3R1

5295

Affinity Capture-MS		Homo sapiens
Affinity Capture-Luminescence		Homo sapiens
Affinity Capture-MS		Homo sapiens
Affinity Capture-MS		Homo sapiens

CUL2

8453

Affinity Capture-MS

Homo sapiens

CEP76

Affinity Capture-MS

Homo sapiens

HDAC5

10014

Affinity Capture-MS

Homo sapiens

HUWE1

10075

Affinity Capture-MS

Homo sapiens

UBXN6

80700

Affinity Capture-MS

Homo sapiens

CACYBP

27101

Affinity Capture-Luminescence		Homo sapiens
Affinity Capture-MS		Homo sapiens

PIK3CB

5291

Affinity Capture-MS

Homo sapiens

KDF1

Affinity Capture-MS

Homo sapiens

YWHAE

7531

Affinity Capture-Luminescence		Homo sapiens
Affinity Capture-MS		Homo sapiens

HSPA5

3309

Cross-Linking-MS (XL-MS)

Homo sapiens

ZBTB2

57621

Affinity Capture-MS

Homo sapiens

AMBRA1

Affinity Capture-MS

Homo sapiens

100

RAB5A

5868

Proximity Label-MS

Homo sapiens

101

RC3H2

Affinity Capture-MS

Homo sapiens

102

SIRT7

Affinity Capture-MS

Homo sapiens

103

LAMP1

3916

Proximity Label-MS

Homo sapiens

104

CUL3

8452

Affinity Capture-MS		Homo sapiens
Affinity Capture-MS		Homo sapiens

105

PTEN

5728

Affinity Capture-Western		Homo sapiens
Affinity Capture-MS		Homo sapiens

106

MTNR1A

Affinity Capture-MS		Homo sapiens
Reconstituted Complex		Homo sapiens

107

EGFR

1956

Affinity Capture-MS		Homo sapiens
Protein-peptide		Homo sapiens

108

ARF6

382

Proximity Label-MS

Homo sapiens

109

GBF1

8729

Affinity Capture-Luminescence		Homo sapiens
Affinity Capture-MS		Homo sapiens

110

DUSP16

Affinity Capture-MS

Homo sapiens

111

RIPK4

Affinity Capture-MS

Homo sapiens

112

RCN1

5954

Affinity Capture-Luminescence		Homo sapiens
Affinity Capture-MS		Homo sapiens

113

GPRASP2

Affinity Capture-Luminescence		Homo sapiens
Affinity Capture-MS		Homo sapiens

114

PLEKHA4

57664

Affinity Capture-MS

Homo sapiens

115

AURKB

9212

Affinity Capture-MS

Homo sapiens

116

CEP250

11190

Affinity Capture-MS

Homo sapiens

117

MAPK6

Affinity Capture-MS

Homo sapiens

118

MYC

Affinity Capture-MS		Homo sapiens
Affinity Capture-MS		Homo sapiens

119

RPA3

6119

Proximity Label-MS

Homo sapiens

120

SAV1

Affinity Capture-MS

Homo sapiens

121

CSNK2A2

1459

Affinity Capture-MS

Homo sapiens

122

PIK3CA

5290

Affinity Capture-MS		Homo sapiens
Affinity Capture-MS		Homo sapiens

123

HSPA4

3308

Affinity Capture-MS

Homo sapiens

124

RAF1

5894

Affinity Capture-Luminescence		Homo sapiens
Affinity Capture-MS		Homo sapiens

125

RCN2

5955

Affinity Capture-Luminescence		Homo sapiens
Affinity Capture-MS		Homo sapiens

126

DDRGK1

65992

Affinity Capture-MS		Homo sapiens
Affinity Capture-MS		Homo sapiens

127

RHOB

388

Proximity Label-MS

Homo sapiens

128

ILK

3611

Affinity Capture-MS

Homo sapiens

129

PTGES3

10728

Affinity Capture-Luminescence		Homo sapiens
Affinity Capture-MS		Homo sapiens

130

HAX1

Proximity Label-MS

Homo sapiens

131

FLOT1

10211

Proximity Label-MS

Homo sapiens

132

CCDC8

Affinity Capture-MS

Homo sapiens

133

RNF43

Proximity Label-MS

Homo sapiens

134

Asb4

Affinity Capture-Western

Mus musculus

135

TERF2

Affinity Capture-MS

Homo sapiens

136

SIRT6

Affinity Capture-MS

Homo sapiens

137

LAMTOR1

55004

Proximity Label-MS

Homo sapiens

138

GRB2

2885

Affinity Capture-MS		Homo sapiens
Affinity Capture-Western		Homo sapiens
Affinity Capture-Western		Homo sapiens

139

FBXL6

Affinity Capture-MS

Homo sapiens

140

TP53

7157

Affinity Capture-MS

Homo sapiens

141

TUBG1

7283

Affinity Capture-MS

Homo sapiens

142

DNAJA2

10294

Affinity Capture-Luminescence		Homo sapiens
Affinity Capture-MS		Homo sapiens

143

ZNF746

Affinity Capture-MS

Homo sapiens

144

USP36

Affinity Capture-MS

Homo sapiens

145

PDHA1

5160

Affinity Capture-MS

Homo sapiens

146

LCK

3932

Proximity Label-MS

Homo sapiens

147

DNAJA3

9093

Affinity Capture-Luminescence		Homo sapiens
Affinity Capture-MS		Homo sapiens

148

HSPB1

3315

Affinity Capture-Luminescence		Homo sapiens
Affinity Capture-MS		Homo sapiens

149

NISCH

11188

Affinity Capture-MS		Homo sapiens
Affinity Capture-Western		Homo sapiens
Affinity Capture-Luminescence		Homo sapiens
Affinity Capture-MS		Homo sapiens

150

HSP90AA1

3320

Affinity Capture-MS		Homo sapiens
Affinity Capture-MS		Homo sapiens

151

FKBP5

2289

Affinity Capture-Luminescence		Homo sapiens
Affinity Capture-MS		Homo sapiens

152

C11orf52

91894

Proximity Label-MS

Homo sapiens

153

TUBB4A

10382

Affinity Capture-Luminescence		Homo sapiens
Affinity Capture-MS		Homo sapiens

154

AARSD1

80755

Affinity Capture-Luminescence		Homo sapiens
Affinity Capture-MS		Homo sapiens

155

CSNK2A1

1457

Affinity Capture-MS

Homo sapiens

156

RNF126

Affinity Capture-MS

Homo sapiens

157

UFL1

23376

Affinity Capture-MS

Homo sapiens

158

FAM27E3

Affinity Capture-Luminescence		Homo sapiens
Affinity Capture-MS		Homo sapiens

159

TERF1

7013

Affinity Capture-MS

Homo sapiens

160

CALU

813

Affinity Capture-Luminescence		Homo sapiens
Affinity Capture-MS		Homo sapiens

161

MAGED1

9500

Affinity Capture-Luminescence		Homo sapiens
Affinity Capture-MS		Homo sapiens

162

RAB2A

5862

Proximity Label-MS

Homo sapiens

163

BIN1

274

Affinity Capture-Luminescence		Homo sapiens
Affinity Capture-MS		Homo sapiens

164

NEDD1

121441

Affinity Capture-MS

Homo sapiens

165

PINK1

Affinity Capture-MS		Homo sapiens
Affinity Capture-MS		Homo sapiens

166

EPHA2

1969

Proximity Label-MS

Homo sapiens

167

PIK3R2

5296

Affinity Capture-MS		Homo sapiens
Affinity Capture-MS		Homo sapiens
Affinity Capture-Luminescence		Homo sapiens
Affinity Capture-MS		Homo sapiens

168

KRAS

3845

Proximity Label-MS

Homo sapiens

169

RAB5C

5878

Proximity Label-MS

Homo sapiens

170

C9orf72

Affinity Capture-MS

Homo sapiens

171

PPP2R1A

5518

Affinity Capture-MS

Homo sapiens

172

ATXN3

4287

Affinity Capture-MS

Homo sapiens

173

STX4

6810

Proximity Label-MS

Homo sapiens

174

PTPN18

26469

Affinity Capture-MS

Homo sapiens

175

PRMT1

3276

Affinity Capture-MS

Homo sapiens

176

CAV1

857

Proximity Label-MS

Homo sapiens

177

RANGAP1

5905

Cross-Linking-MS (XL-MS)

Homo sapiens

178

DERL1

79139

Affinity Capture-MS

Homo sapiens

179

PIK3R3

8503

Affinity Capture-MS		Homo sapiens
Affinity Capture-Luminescence		Homo sapiens
Affinity Capture-MS		Homo sapiens

View the network

Pathways in which IRS4 is involved

Pathway	Evidence	Source
IGF1R signaling cascade	TAS	Reactome
IRS-related events triggered by IGF1R	TAS	Reactome
Signal Transduction	TAS	Reactome
Signaling by Receptor Tyrosine Kinases	TAS	Reactome
Signaling by Type 1 Insulin-like Growth Factor 1 Receptor (IGF1R)	TAS	Reactome

Perform bioinformatics analysis of your extracellular vesicle data set using FunRich, a open access standalone tool. NEW UPDATED VERSION OF FunRich available for download (12/09/2016) from here