Gene description for DNAH17
Gene name dynein, axonemal, heavy chain 17
Gene symbol DNAH17
Other names/aliases DNAHL1
DNEL2
Species Homo sapiens
 Database cross references - DNAH17
ExoCarta ExoCarta_8632
Entrez Gene 8632
HGNC 2946
MIM 610063
 DNAH17 identified in exosomes derived from the following tissue/cell type
Hepatocytes 26054723    
Neuroblastoma cells 25944692    
 Gene ontology annotations for DNAH17
Molecular Function
    ATPase activity GO:0016887 IEA
    ATP binding GO:0005524 IEA
    microtubule motor activity GO:0003777 NAS
Biological Process
    metabolic process GO:0008152 NAS
    cilium or flagellum-dependent cell motility GO:0001539 NAS
    microtubule-based movement GO:0007018 IEA
Subcellular Localization
    microtubule GO:0005874 IEA
    axonemal dynein complex GO:0005858 NAS
 Experiment description of studies that identified DNAH17 in exosomes
1
Experiment ID 237
ISEV standards
EM
EV Biophysical techniques
TSG101|Alix|HSC70|GAPDH
EV Cytosolic markers
EV Membrane markers
HSP90B1
EV Negative markers
qNano
EV Particle analysis
Identified molecule mRNA
Identification method RNA Sequencing
PubMed ID 26054723    
Organism Homo sapiens
Experiment description Hepatocellular carcinoma-derived exosomes promote motility of immortalized hepatocyte through transfer of oncogenic proteins and RNAs
Authors He M, Qin H, Poon TC, Sze SC, Ding X, Co NN, Ngai SM, Chan TF, Wong N
Journal name Carcinogenesis
Publication year 2015
Sample Hepatocytes
Sample name MIHA
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
RNA
Methods used in the study Western blotting
Mass spectrometry
RNA Sequencing
2
Experiment ID 224
ISEV standards
EM|AFM
EV Biophysical techniques
Alix|TSG101
EV Cytosolic markers
CD63|CD81
EV Membrane markers
GOLGA2
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25944692    
Organism Homo sapiens
Experiment description Proteogenomic analysis reveals exosomes are more oncogenic than ectosomes
Authors Keerthikumar S, Gangoda L, Liem M, Fonseka P, Atukorala I, Ozcitti C, Mechler A, Adda CG, Ang CS, Mathivanan S
Journal name Oncotarget
Publication year 2015
Sample Neuroblastoma cells
Sample name SH-SY5Y
Isolation/purification methods Differential centrifugation
Ultracentrifugation
OptiPrep density gradient
Flotation density 1.10 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
Western blotting
 Protein-protein interactions for DNAH17
  Protein Interactor ExoCarta ID Identification method PubMed Species
No interactions are found.
 Pathways in which DNAH17 is involved
No pathways found





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