Gene description for PIK3CA
Gene name phosphatidylinositol-4,5-bisphosphate 3-kinase, catalytic subunit alpha
Gene symbol PIK3CA
Other names/aliases CLOVE
CWS5
MCAP
MCM
MCMTC
PI3K
p110-alpha
Species Homo sapiens
 Database cross references - PIK3CA
ExoCarta ExoCarta_5290
Vesiclepedia VP_5290
Entrez Gene 5290
HGNC 8975
MIM 171834
UniProt P42336  
 PIK3CA identified in exosomes derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Mesenchymal stem cells 36408942    
Nasopharyngeal carcinoma cells 21059916    
 Gene ontology annotations for PIK3CA
Molecular Function
    phosphatidylinositol 3-kinase activity GO:0035004 TAS
    protein serine/threonine kinase activity GO:0004674 IEA
    kinase activity GO:0016301 TAS
    phosphatidylinositol-4,5-bisphosphate 3-kinase activity GO:0046934 TAS
    1-phosphatidylinositol-4-phosphate 3-kinase activity GO:0035005 IBA
    protein kinase activator activity GO:0030295 IEA
    insulin receptor substrate binding GO:0043560 IEA
    protein binding GO:0005515 IPI
    ATP binding GO:0005524 IEA
    1-phosphatidylinositol-3-kinase activity GO:0016303 IDA
Biological Process
    phospholipid metabolic process GO:0006644 TAS
    protein phosphorylation GO:0006468 IBA
    positive regulation of protein kinase activity GO:0045860 IEA
    phosphatidylinositol 3-kinase signaling GO:0014065 IBA
    insulin receptor signaling pathway GO:0008286 TAS
    activation of mitophagy in response to mitochondrial depolarization GO:0098779 IMP
    T cell costimulation GO:0031295 TAS
    epidermal growth factor receptor signaling pathway GO:0007173 TAS
    cell chemotaxis GO:0060326 IBA
    hypomethylation of CpG island GO:0044029 IEA
    negative regulation of anoikis GO:2000811 IMP
    inflammatory response GO:0006954 IBA
    small molecule metabolic process GO:0044281 TAS
    negative regulation of fibroblast apoptotic process GO:2000270 IEA
    innate immune response GO:0045087 TAS
    adaptive immune response GO:0002250 IBA
    vasculature development GO:0001944 TAS
    phosphatidylinositol phosphorylation GO:0046854 ISS
    T cell receptor signaling pathway GO:0050852 TAS
    cardiac muscle contraction GO:0060048 TAS
    phosphatidylinositol biosynthetic process GO:0006661 TAS
    phosphatidylinositol-3-phosphate biosynthetic process GO:0036092 IBA
    leukocyte migration GO:0050900 TAS
    platelet activation GO:0030168 TAS
    negative regulation of neuron apoptotic process GO:0043524 IEA
    positive regulation of peptidyl-serine phosphorylation GO:0033138 IEA
    Fc-epsilon receptor signaling pathway GO:0038095 TAS
    vascular endothelial growth factor receptor signaling pathway GO:0048010 TAS
    Fc-gamma receptor signaling pathway involved in phagocytosis GO:0038096 TAS
    angiogenesis GO:0001525 IEA
    glucose metabolic process GO:0006006 IEA
    neurotrophin TRK receptor signaling pathway GO:0048011 TAS
    endothelial cell migration GO:0043542 TAS
    regulation of genetic imprinting GO:2000653 IEA
    regulation of multicellular organism growth GO:0040014 IEA
    protein kinase B signaling GO:0043491 IEA
    phosphatidylinositol-mediated signaling GO:0048015 TAS
    insulin receptor signaling pathway via phosphatidylinositol 3-kinase GO:0038028 TAS
    blood coagulation GO:0007596 TAS
    fibroblast growth factor receptor signaling pathway GO:0008543 TAS
Subcellular Localization
    phosphatidylinositol 3-kinase complex, class IA GO:0005943 IDA
    plasma membrane GO:0005886 IBA
    phosphatidylinositol 3-kinase complex GO:0005942 ISS
    cytoplasm GO:0005737 IBA
    cytosol GO:0005829 TAS
    lamellipodium GO:0030027 IEA
 Experiment description of studies that identified PIK3CA in exosomes
1
Experiment ID 489
MISEV standards
✔
EM
EV Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
EV Enriched markers
✔
Canx
EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ.
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
✔
EM
EV Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
EV Enriched markers
✔
Canx
EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ.
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
✔
EM
EV Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
EV Enriched markers
✔
Canx
EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ.
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
✔
EM
EV Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
EV Enriched markers
✔
Canx
EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ.
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
5
Experiment ID 488
MISEV standards
✔
EM
EV Biophysical techniques
✔
CD9|CD81|CD63|GAPDH|SDCBP|LAMP1|TFRC|UCHL1|FLOT2|LAMP2|FLOT1|ICAM1|RAB5B|CD151|RAB35|TSG101|RAB5A|CD82
EV Enriched markers
✔
CANX
EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Homo sapiens
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ.
Journal name Proteomics
Publication year 2023
Sample Mesenchymal stem cells
Sample name UCMSC
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
6
Experiment ID 125
MISEV standards
✘ EV Biophysical techniques
✔
HSC70|GAPDH|FLOT2
EV Enriched markers
✔
HSPA5
EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Western blotting
PubMed ID 21059916    
Organism Homo sapiens
Experiment description Human tumor virus utilizes exosomes for intercellular communication.
Authors Meckes DG Jr, Shair KH, Marquitz AR, Kung CP, Edwards RH, Raab-Traub N.
Journal name PNAS
Publication year 2010
Sample Nasopharyngeal carcinoma cells
Sample name C666
Isolation/purification methods Differential centrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
 Protein-protein interactions for PIK3CA
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 YWHAH 7533
Affinity Capture-Western Homo sapiens
2 GSN 2934
Affinity Capture-Western Homo sapiens
3 ATR  
Protein-peptide Homo sapiens
4 RASGRP3  
Biochemical Activity Homo sapiens
5 PRKCD 5580
Invitro Homo sapiens
6 GNAQ 2776
Affinity Capture-Western Homo sapiens
7 IRS4 8471
Invivo Homo sapiens
8 LCK 3932
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Invitro Homo sapiens
9 HRAS 3265
Two-hybrid Homo sapiens
Invivo Homo sapiens
Reconstituted Complex Homo sapiens
10 SLC9A3R2 9351
Reconstituted Complex Homo sapiens
11 CBL 867
Co-purification Homo sapiens
12 ARHGEF1 9138
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
13 NOTCH1 4851
Affinity Capture-Western Homo sapiens
14 DGKZ  
Affinity Capture-Western Homo sapiens
Biochemical Activity Homo sapiens
15 NRAS 4893
Invivo Homo sapiens
Invitro Homo sapiens
16 RASD2  
Reconstituted Complex Homo sapiens
17 SLC9A3R1 9368
Reconstituted Complex Homo sapiens
18 YWHAQ 10971
Reconstituted Complex Homo sapiens
19 CENTA1  
Reconstituted Complex Homo sapiens
Biochemical Activity Homo sapiens
20 KRAS 3845
Two-hybrid Homo sapiens
21 PIK3R1 5295
Reconstituted Complex Homo sapiens
22 IRS1 3667
Affinity Capture-Western Homo sapiens
23 SH3KBP1 30011
Invivo Homo sapiens
Invitro Homo sapiens
24 PIK3R3 8503
Two-hybrid Homo sapiens
View the network image/svg+xml
 Pathways in which PIK3CA is involved
PathwayEvidenceSource
CD28 dependent PI3K/Akt signaling TAS Reactome
Constitutive Signaling by Aberrant PI3K in Cancer TAS Reactome
Constitutive Signaling by EGFRvIII TAS Reactome
Constitutive Signaling by Ligand-Responsive EGFR Cancer Variants TAS Reactome
Costimulation by the CD28 family TAS Reactome
DAP12 signaling TAS Reactome
Downstream signal transduction TAS Reactome
Downstream TCR signaling TAS Reactome
G alpha (12/13) signalling events TAS Reactome
G alpha (q) signalling events TAS Reactome
G alpha (q) signalling events TAS Reactome
G beta:gamma signalling through PI3Kgamma TAS Reactome
GAB1 signalosome TAS Reactome
GPVI-mediated activation cascade TAS Reactome
Interleukin receptor SHC signaling TAS Reactome
Interleukin receptor SHC signaling TAS Reactome
Interleukin-3, 5 and GM-CSF signaling TAS Reactome
Nephrin interactions TAS Reactome
PI-3K cascade:FGFR1 TAS Reactome
PI-3K cascade:FGFR2 TAS Reactome
PI-3K cascade:FGFR3 TAS Reactome
PI-3K cascade:FGFR4 TAS Reactome
PI3K Cascade TAS Reactome
PI3K Cascade TAS Reactome
PI3K events in ERBB2 signaling TAS Reactome
PI3K events in ERBB4 signaling TAS Reactome
PI3K/AKT activation TAS Reactome
PIP3 activates AKT signaling TAS Reactome
Regulation of signaling by CBL TAS Reactome
Role of LAT2/NTAL/LAB on calcium mobilization TAS Reactome
Role of phospholipids in phagocytosis TAS Reactome
Signaling by FGFR1 fusion mutants TAS Reactome
Signaling by FGFR1 mutants TAS Reactome
Signaling by FGFR2 mutants TAS Reactome
Signaling by FGFR3 mutants TAS Reactome
Signaling by FGFR4 mutants TAS Reactome
Signaling by SCF-KIT TAS Reactome
Synthesis of PIPs at the plasma membrane TAS Reactome
Tie2 Signaling TAS Reactome
VEGFA-VEGFR2 Pathway TAS Reactome





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