Gene description for AMBP
Gene name alpha-1-microglobulin/bikunin precursor
Gene symbol AMBP
Other names/aliases A1M
EDC1
HCP
HI30
IATIL
ITI
ITIL
ITILC
UTI
Species Homo sapiens
 Database cross references - AMBP
ExoCarta ExoCarta_259
Entrez Gene 259
HGNC 453
MIM 176870
UniProt P02760  
 AMBP identified in exosomes derived from the following tissue/cell type
Hepatocellular carcinoma cells 26054723    
Urine 15326289    
Urine 19056867    
Urine 21557262    
 Gene ontology annotations for AMBP
Molecular Function
    heme binding GO:0020037 IDA
    protein binding GO:0005515 IPI
    calcium oxalate binding GO:0046904 NAS
    calcium channel inhibitor activity GO:0019855 NAS
    protein homodimerization activity GO:0042803 IPI
    serine-type endopeptidase inhibitor activity GO:0004867 TAS
    IgA binding GO:0019862 IDA
    small molecule binding GO:0036094 IEA
Biological Process
    female pregnancy GO:0007565 NAS
    heme catabolic process GO:0042167 NAS
    receptor-mediated endocytosis GO:0006898 TAS
    cell adhesion GO:0007155 NAS
    protein catabolic process GO:0030163 IEA
    viral process GO:0016032 IEA
    negative regulation of immune response GO:0050777 NAS
    negative regulation of endopeptidase activity GO:0010951 TAS
    negative regulation of JNK cascade GO:0046329 TAS
    protein-chromophore linkage GO:0018298 IEA
Subcellular Localization
    extracellular region GO:0005576 TAS
    blood microparticle GO:0072562 IDA
    cell surface GO:0009986 IEA
    intracellular membrane-bounded organelle GO:0043231 IEA
    extracellular space GO:0005615 IDA
    extracellular exosome GO:0070062 IDA
    plasma membrane GO:0005886 IDA
 Experiment description of studies that identified AMBP in exosomes
1
Experiment ID 235
ISEV standards
✔
EM
EV Biophysical techniques
✔
TSG101|Alix|HSC70|GAPDH
EV Cytosolic markers
✘ EV Membrane markers
✔
HSP90B1
EV Negative markers
✔
qNano
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 26054723    
Organism Homo sapiens
Experiment description Hepatocellular carcinoma-derived exosomes promote motility of immortalized hepatocyte through transfer of oncogenic proteins and RNAs
Authors He M, Qin H, Poon TC, Sze SC, Ding X, Co NN, Ngai SM, Chan TF, Wong N
Journal name Carcinogenesis
Publication year 2015
Sample Hepatocellular carcinoma cells
Sample name HKCI-8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
RNA
Methods used in the study Western blotting
Mass spectrometry
RT-PCR
RNA Sequencing
2
Experiment ID 13
ISEV standards
✔
IEM
EV Biophysical techniques
✔
Alix|RAB4|RAB5B|RAB11|TSG101
EV Cytosolic markers
✔
CD9|AQP2|AQP1
EV Membrane markers
✘ EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 15326289    
Organism Homo sapiens
Experiment description Identification and proteomic profiling of exosomes in human urine.
Authors Pisitkun T, Shen RF, Knepper MA
Journal name PNAS
Publication year 2004
Sample Urine
Sample name Urine - Normal
Isolation/purification methods Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [LCQ DECA XP]
Western blotting
3
Experiment ID 63
ISEV standards
✘ EV Biophysical techniques
✘ EV Cytosolic markers
✔
AQP2
EV Membrane markers
✘ EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 19056867    
Organism Homo sapiens
Experiment description Large-scale proteomics and phosphoproteomics of urinary exosomes.
Authors Gonzales PA, Pisitkun T, Hoffert JD, Tchapyjnikov D, Star RA, Kleta R, Wang NS, Knepper MA
Journal name JASN
Publication year 2009
Sample Urine
Sample name Urine - Normal
Isolation/purification methods Differential centrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [LTQ]
Western blotting
4
Experiment ID 114
ISEV standards
✔
IEM
EV Biophysical techniques
✘ EV Cytosolic markers
✘ EV Membrane markers
✘ EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 21557262    
Organism Homo sapiens
Experiment description Proteomic identification of exosomal LRG1: A potential urinary biomarker for detecting NSCLC.
Authors Li Y, Zhang Y, Qiu F, Qiu Z.
Journal name ELEC
Publication year 2011
Sample Urine
Sample name Urine - Lung cancer
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
Western blotting
 Protein-protein interactions for AMBP
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 CD79A  
Co-purification Homo sapiens
Affinity Capture-Western Homo sapiens
2 A2M 2
Invivo Homo sapiens
Invitro Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
3 ALB 213
Affinity Capture-Western Homo sapiens
4 AMBP 259
Two-hybrid Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
Two-hybrid Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
5 FN1 2335
Affinity Capture-Western Homo sapiens
View the network image/svg+xml
 Pathways in which AMBP is involved
PathwayEvidenceSource
Scavenging of heme from plasma TAS Reactome





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