Gene description for AMBP
Gene name alpha-1-microglobulin/bikunin precursor
Gene symbol AMBP
Other names/aliases A1M
EDC1
HCP
HI30
IATIL
ITI
ITIL
ITILC
UTI
Species Homo sapiens
 Database cross references - AMBP
ExoCarta ExoCarta_259
Vesiclepedia VP_259
Entrez Gene 259
HGNC 453
MIM 176870
UniProt P02760  
 AMBP identified in sEVs derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Hepatocellular carcinoma cells 26054723    
Urine 15326289    
Urine 19056867    
Urine 21557262    
 Gene ontology annotations for AMBP
Molecular Function
    serine-type endopeptidase inhibitor activity GO:0004867 IBA
    serine-type endopeptidase inhibitor activity GO:0004867 IDA
    serine-type endopeptidase inhibitor activity GO:0004867 ISS
    serine-type endopeptidase inhibitor activity GO:0004867 TAS
    protein binding GO:0005515 IPI
    antioxidant activity GO:0016209 IDA
    oxidoreductase activity GO:0016491 IEA
    oxidoreductase activity, acting on NAD(P)H, heme protein as acceptor GO:0016653 IDA
    calcium channel inhibitor activity GO:0019855 NAS
    IgA binding GO:0019862 IDA
    heme binding GO:0020037 IDA
    carbohydrate binding GO:0030246 EXP
    chondroitin sulfate binding GO:0035374 IDA
    protein homodimerization activity GO:0042803 IDA
    protein homodimerization activity GO:0042803 IPI
    calcium oxalate binding GO:0046904 NAS
    collagen fibril binding GO:0098633 IDA
Biological Process
    cell adhesion GO:0007155 NAS
    female pregnancy GO:0007565 NAS
    hemoglobin metabolic process GO:0020027 IDA
    cellular response to reactive oxygen species GO:0034614 IDA
    heme catabolic process GO:0042167 NAS
    negative regulation of JNK cascade GO:0046329 TAS
    negative regulation of immune response GO:0050777 NAS
    cellular oxidant detoxification GO:0098869 IDA
    cytochrome c metabolic process GO:1903606 IDA
Subcellular Localization
    extracellular region GO:0005576 NAS
    extracellular region GO:0005576 TAS
    extracellular space GO:0005615 HDA
    extracellular space GO:0005615 IDA
    mitochondrial inner membrane GO:0005743 IEA
    endoplasmic reticulum GO:0005783 IEA
    cytosol GO:0005829 IEA
    plasma membrane GO:0005886 IBA
    plasma membrane GO:0005886 IDA
    cell surface GO:0009986 IBA
    nuclear membrane GO:0031965 IEA
    collagen-containing extracellular matrix GO:0062023 HDA
    extracellular exosome GO:0070062 HDA
    blood microparticle GO:0072562 HDA
    matrix side of mitochondrial inner membrane GO:0099617 IDA
 Experiment description of studies that identified AMBP in sEVs
1
Experiment ID 489
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
5
Experiment ID 235
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|Alix|HSC70|GAPDH
Enriched markers
✔
HSP90B1
Negative markers
✔
qNano
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 26054723    
Organism Homo sapiens
Experiment description Hepatocellular carcinoma-derived exosomes promote motility of immortalized hepatocyte through transfer of oncogenic proteins and RNAs
Authors "He M, Qin H, Poon TC, Sze SC, Ding X, Co NN, Ngai SM, Chan TF, Wong N"
Journal name Carcinogenesis
Publication year 2015
Sample Hepatocellular carcinoma cells
Sample name HKCI-8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
RNA
Methods used in the study Western blotting
Mass spectrometry
RT-PCR
RNA Sequencing
6
Experiment ID 13
MISEV standards
✔
IEM
Biophysical techniques
✔
Alix|RAB4|RAB5B|RAB11|TSG101|CD9|AQP2|AQP1
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 15326289    
Organism Homo sapiens
Experiment description Identification and proteomic profiling of exosomes in human urine.
Authors "Pisitkun T, Shen RF, Knepper MA"
Journal name PNAS
Publication year 2004
Sample Urine
Sample name Urine - Normal
Isolation/purification methods Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [LCQ DECA XP]
Western blotting
7
Experiment ID 63
MISEV standards
✘ Biophysical techniques
✔
AQP2
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 19056867    
Organism Homo sapiens
Experiment description Large-scale proteomics and phosphoproteomics of urinary exosomes.
Authors "Gonzales PA, Pisitkun T, Hoffert JD, Tchapyjnikov D, Star RA, Kleta R, Wang NS, Knepper MA"
Journal name JASN
Publication year 2009
Sample Urine
Sample name Urine - Normal
Isolation/purification methods Differential centrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [LTQ]
Western blotting
8
Experiment ID 114
MISEV standards
✔
IEM
Biophysical techniques
✘ Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 21557262    
Organism Homo sapiens
Experiment description Proteomic identification of exosomal LRG1: A potential urinary biomarker for detecting NSCLC.
Authors "Li Y, Zhang Y, Qiu F, Qiu Z."
Journal name ELEC
Publication year 2011
Sample Urine
Sample name Urine - Lung cancer
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
Western blotting
 Protein-protein interactions for AMBP
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 A2M 2
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
2 FN1 2335
Affinity Capture-Western Homo sapiens
3 RPA4  
Proximity Label-MS Homo sapiens
4 ENO1 2023
Two-hybrid Homo sapiens
5 ALB 213
Affinity Capture-Western Homo sapiens
6 STAT3 6774
Two-hybrid Homo sapiens
7 AMBP 259
Two-hybrid Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
Two-hybrid Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
8 ITIH1 3697
Cross-Linking-MS (XL-MS) Homo sapiens
9 FHL3 2275
Two-hybrid Homo sapiens
10 ITIH2 3698
Cross-Linking-MS (XL-MS) Homo sapiens
11 CTDP1 9150
Affinity Capture-MS Homo sapiens
12 COX8A  
Two-hybrid Homo sapiens
13 CD79A  
Co-purification Homo sapiens
Affinity Capture-Western Homo sapiens
14 GRB2 2885
Two-hybrid Homo sapiens
15 DAAM1 23002
Two-hybrid Homo sapiens
16 PIK3R3 8503
Two-hybrid Homo sapiens
17 PIK3CA 5290
Two-hybrid Homo sapiens
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