Gene description for FHL3
Gene name four and a half LIM domains 3
Gene symbol FHL3
Other names/aliases SLIM2
Species Homo sapiens
 Database cross references - FHL3
ExoCarta ExoCarta_2275
Vesiclepedia VP_2275
Entrez Gene 2275
HGNC 3704
MIM 602790
UniProt Q13643  
 FHL3 identified in exosomes derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Mesenchymal stem cells 36408942    
 Gene ontology annotations for FHL3
Molecular Function
    transcription coregulator activity GO:0003712 IBA
    actin binding GO:0003779 IBA
    protein binding GO:0005515 IPI
    metal ion binding GO:0046872 IEA
Biological Process
    muscle organ development GO:0007517 TAS
    actin cytoskeleton organization GO:0030036 IBA
Subcellular Localization
    stress fiber GO:0001725 IBA
    nucleus GO:0005634 IBA
    focal adhesion GO:0005925 HDA
    Z disc GO:0030018 IBA
 Experiment description of studies that identified FHL3 in exosomes
1
Experiment ID 489
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
5
Experiment ID 488
MISEV standards
✔
EM
Biophysical techniques
✔
CD9|CD81|CD63|GAPDH|SDCBP|LAMP1|TFRC|UCHL1|FLOT2|LAMP2|FLOT1|ICAM1|RAB5B|CD151|RAB35|TSG101|RAB5A|CD82
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Homo sapiens
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Mesenchymal stem cells
Sample name UCMSC
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
 Protein-protein interactions for FHL3
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 CNOT7 29883
Two-hybrid Homo sapiens
2 CCAR1 55749
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
3 MYBPHL  
Two-hybrid Homo sapiens
4 C19orf68  
Affinity Capture-MS Homo sapiens
5 SLIRP 81892
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
6 TMEM14B  
Two-hybrid Homo sapiens
7 THSD7A 221981
Affinity Capture-MS Homo sapiens
8 C14orf105  
Two-hybrid Homo sapiens
9 PRR35  
Two-hybrid Homo sapiens
10 SNRPG 6637
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
11 DCDC2B  
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
12 WNK1 65125
Two-hybrid Homo sapiens
Affinity Capture-MS Homo sapiens
13 PKP2 5318
Affinity Capture-MS Homo sapiens
14 CTBP2 1488
Two-hybrid Homo sapiens
Reconstituted Complex Homo sapiens
15 P4HA2 8974
Two-hybrid Homo sapiens
16 QKI 9444
Two-hybrid Homo sapiens
17 PLEKHF2 79666
Two-hybrid Homo sapiens
18 MSRB3 253827
Two-hybrid Homo sapiens
19 GBX2  
Affinity Capture-MS Homo sapiens
20 MRPL27 51264
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
21 UBC 7316
Affinity Capture-MS Homo sapiens
22 FAM71C  
Two-hybrid Homo sapiens
23 RPUSD4 84881
Proximity Label-MS Homo sapiens
24 EIF4EBP2 1979
Two-hybrid Homo sapiens
25 TJAP1 93643
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
26 HIPK1 204851
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
27 THSD4 79875
Affinity Capture-MS Homo sapiens
28 SHE  
Two-hybrid Homo sapiens
29 HAPLN2  
Two-hybrid Homo sapiens
30 AMOTL1 154810
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
31 FGFR3 2261
Affinity Capture-MS Homo sapiens
32 MYOZ3  
Two-hybrid Homo sapiens
33 DPPA4  
Affinity Capture-MS Homo sapiens
34 CAPZB 832
Affinity Capture-MS Homo sapiens
35 ITGA7 3679
Two-hybrid Homo sapiens
Affinity Capture-Western Homo sapiens
Co-localization Homo sapiens
36 RHEBL1  
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
37 TGFB1 7040
Affinity Capture-MS Homo sapiens
38 ZBTB21  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
39 EPM2AIP1 9852
Two-hybrid Homo sapiens
40 RASL12 51285
Two-hybrid Homo sapiens
41 KDM2A  
Affinity Capture-MS Homo sapiens
42 LASP1 3927
Two-hybrid Homo sapiens
43 HYAL2 8692
Two-hybrid Homo sapiens
44 SNRPC 6631
Two-hybrid Homo sapiens
45 RECQL4  
Affinity Capture-MS Homo sapiens
46 TRO  
Two-hybrid Homo sapiens
47 ARHGEF10 9639
Two-hybrid Homo sapiens
48 MMP14 4323
Two-hybrid Homo sapiens
49 ZNF512B  
Two-hybrid Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-MS Homo sapiens
50 UBE2Q1 55585
Two-hybrid Homo sapiens
51 HIPK2 28996
Affinity Capture-MS Homo sapiens
52 SLC25A46 91137
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
53 CUL4B 8450
Affinity Capture-MS Homo sapiens
54 THEG  
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
55 ARID5A  
Two-hybrid Homo sapiens
56 GUCD1  
Two-hybrid Homo sapiens
57 RBM42  
Two-hybrid Homo sapiens
58 SDCBP 6386
Two-hybrid Homo sapiens
59 STYX  
Affinity Capture-MS Homo sapiens
60 SLU7  
Two-hybrid Homo sapiens
61 LMO4  
Two-hybrid Homo sapiens
62 KRTAP12-1  
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
63 LARP4B 23185
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
64 MAP7D2 256714
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
65 FAM154A  
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
66 ATXN2 6311
Affinity Capture-MS Homo sapiens
67 COBL  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
68 PRR5L  
Two-hybrid Homo sapiens
69 ADAMTSL4  
Two-hybrid Homo sapiens
70 ZYX 7791
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
71 PTPN6 5777
Two-hybrid Homo sapiens
72 MTUS2 23281
Two-hybrid Homo sapiens
73 FAM222B  
Two-hybrid Homo sapiens
74 TSGA10IP  
Two-hybrid Homo sapiens
75 BRIP1  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
76 FHL3 2275
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
77 CUL3 8452
Affinity Capture-MS Homo sapiens
78 E2F8  
Two-hybrid Homo sapiens
79 CDKN1A  
Two-hybrid Homo sapiens
80 SBF2 81846
Two-hybrid Homo sapiens
81 ZBTB16  
Phenotypic Enhancement Homo sapiens
82 FAM122A  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
83 FAM110A  
Two-hybrid Homo sapiens
84 SUPT5H 6829
Two-hybrid Homo sapiens
85 UBE2I 7329
Two-hybrid Homo sapiens
86 ODF3B  
Two-hybrid Homo sapiens
87 RNF146  
Affinity Capture-Western Homo sapiens
88 CNNM3 26505
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
89 CORO1A 11151
Two-hybrid Homo sapiens
90 SLAIN1 122060
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
91 SREBF2 6721
Two-hybrid Homo sapiens
92 RFX3 5991
Two-hybrid Homo sapiens
93 TYK2 7297
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
94 CYSRT1 375791
Two-hybrid Homo sapiens
95 PTP4A2 8073
Affinity Capture-MS Homo sapiens
96 EXOSC5 56915
Two-hybrid Homo sapiens
97 PATL1 219988
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
98 PML 5371
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
99 FAM98C  
Affinity Capture-MS Homo sapiens
100 GATA2  
Two-hybrid Homo sapiens
101 QRICH1  
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-MS Homo sapiens
102 PAK7  
Two-hybrid Homo sapiens
103 THAP7  
Two-hybrid Homo sapiens
104 KCTD3  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
105 KLF12  
Two-hybrid Homo sapiens
106 QSER1  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
107 AIMP2 7965
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
108 MED15  
Two-hybrid Homo sapiens
109 ZMYM4  
Affinity Capture-MS Homo sapiens
110 ADAM15 8751
Two-hybrid Homo sapiens
111 MAP7 9053
Affinity Capture-MS Homo sapiens
112 MRRF  
Two-hybrid Homo sapiens
113 TMX3 54495
Two-hybrid Homo sapiens
114 RAB40B  
Two-hybrid Homo sapiens
115 ATF6  
Affinity Capture-MS Homo sapiens
116 TRIM33 51592
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
117 NPRL3  
Two-hybrid Homo sapiens
118 EFCAB12  
Two-hybrid Homo sapiens
119 ECT2 1894
Affinity Capture-MS Homo sapiens
120 KIRREL2  
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
121 SNRPB 6628
Two-hybrid Homo sapiens
122 MYPOP  
Two-hybrid Homo sapiens
123 TRIP6 7205
Two-hybrid Homo sapiens
124 EPHA10  
Two-hybrid Homo sapiens
125 CDC42EP1 11135
Two-hybrid Homo sapiens
126 WDR25  
Two-hybrid Homo sapiens
127 NEBL 10529
Affinity Capture-MS Homo sapiens
128 SAP30BP  
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
129 AMH  
Affinity Capture-MS Homo sapiens
130 MYBPC1  
Two-hybrid Homo sapiens
Affinity Capture-Western Homo sapiens
131 POM121  
Affinity Capture-MS Homo sapiens
132 TRIM27  
Two-hybrid Homo sapiens
133 FAM90A1  
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
134 RBMS1 5937
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
135 HOXA1 3198
Two-hybrid Homo sapiens
136 MORF4L1  
Two-hybrid Homo sapiens
137 SPATA8  
Two-hybrid Homo sapiens
138 FOSL2  
Two-hybrid Homo sapiens
139 KTI12 112970
Two-hybrid Homo sapiens
140 SLC44A3  
Two-hybrid Homo sapiens
141 SETD1A 9739
Affinity Capture-MS Homo sapiens
142 FAM122B  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
143 MTUS1  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
144 ZMYM6  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
145 PRDM7  
Affinity Capture-MS Homo sapiens
146 PIAS1 8554
Two-hybrid Homo sapiens
147 A1CF  
Two-hybrid Homo sapiens
148 LHB  
Two-hybrid Homo sapiens
149 RFX6  
Two-hybrid Homo sapiens
150 MPZL1 9019
Two-hybrid Homo sapiens
151 PHF21A  
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
152 C1orf94  
Two-hybrid Homo sapiens
153 SH3GLB2 56904
Two-hybrid Homo sapiens
154 ZNF587  
Two-hybrid Homo sapiens
155 PPP1R32  
Two-hybrid Homo sapiens
156 KIAA1671  
Affinity Capture-MS Homo sapiens
157 GCM2  
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
158 AMBP 259
Two-hybrid Homo sapiens
159 CDKN2D  
Two-hybrid Homo sapiens
160 KIDINS220 57498
Two-hybrid Homo sapiens
161 FOXK1 221937
Affinity Capture-MS Homo sapiens
162 FHL2 2274
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
FRET Homo sapiens
163 ZNF417  
Two-hybrid Homo sapiens
164 CSF1 1435
Two-hybrid Homo sapiens
165 ZCCHC14 23174
Two-hybrid Homo sapiens
166 SHKBP1  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
167 IKBKAP 8518
Co-fractionation Homo sapiens
168 TYMSOS  
Two-hybrid Homo sapiens
169 RAD21 5885
Two-hybrid Homo sapiens
Affinity Capture-Western Homo sapiens
Two-hybrid Homo sapiens
170 TRAF3IP2  
Two-hybrid Homo sapiens
171 ADNP 23394
Affinity Capture-MS Homo sapiens
172 PLEKHA5 54477
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
173 TOPBP1 11073
Two-hybrid Homo sapiens
174 ATOH1  
Affinity Capture-MS Homo sapiens
175 MTA1 9112
Two-hybrid Homo sapiens
176 SENP6  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
177 PPIG 9360
Two-hybrid Homo sapiens
178 MKL2  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
179 CRIP3  
Two-hybrid Homo sapiens
180 KLF3  
Affinity Capture-Western Homo sapiens
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
Reconstituted Complex Homo sapiens
Two-hybrid Homo sapiens
181 TANC2  
Affinity Capture-MS Homo sapiens
182 LMO2  
Two-hybrid Homo sapiens
183 YPEL3  
Two-hybrid Homo sapiens
184 CREB1  
Two-hybrid Homo sapiens
Affinity Capture-Western Homo sapiens
185 DDX58 23586
Affinity Capture-RNA Homo sapiens
186 TMEM108  
Two-hybrid Homo sapiens
187 SUGP2  
Affinity Capture-MS Homo sapiens
188 CREB5 9586
Two-hybrid Homo sapiens
189 SRGN 5552
Two-hybrid Homo sapiens
190 RNMTL1  
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
191 KRTAP12-2  
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
192 RYBP  
Two-hybrid Homo sapiens
193 CBX8 57332
Two-hybrid Homo sapiens
194 TTLL10 254173
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
195 CRYBA2  
Two-hybrid Homo sapiens
196 KANK2 25959
Two-hybrid Homo sapiens
197 FBXL18 80028
Two-hybrid Homo sapiens
198 ASCL4  
Two-hybrid Homo sapiens
199 BRD1 23774
Affinity Capture-MS Homo sapiens
200 ZNRD1  
Two-hybrid Homo sapiens