ExoCarta: Gene summary

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Gene description for MRPL27
Gene name	mitochondrial ribosomal protein L27
Gene symbol	MRPL27
Other names/aliases	L27mt
Species	Homo sapiens
Database cross references - MRPL27
ExoCarta	ExoCarta_51264
Vesiclepedia	VP_51264
Entrez Gene	51264
HGNC	14483
MIM	611837
UniProt	Q9P0M9
MRPL27 identified in exosomes derived from the following tissue/cell type
Bone marrow mesenchymal stem cells	36408942
Bone marrow mesenchymal stem cells	36408942
Bone marrow mesenchymal stem cells	36408942
Bone marrow mesenchymal stem cells	36408942

Gene ontology annotations for MRPL27

Molecular Function
	RNA binding	GO:0003723	HDA
	structural constituent of ribosome	GO:0003735	IBA
	structural constituent of ribosome	GO:0003735	NAS
	protein binding	GO:0005515	IPI
Biological Process
	translation	GO:0006412	NAS
	mitochondrial translation	GO:0032543	NAS
Subcellular Localization
	mitochondrion	GO:0005739	HTP
	mitochondrion	GO:0005739	IDA
	mitochondrial inner membrane	GO:0005743	NAS
	mitochondrial inner membrane	GO:0005743	TAS
	mitochondrial large ribosomal subunit	GO:0005762	IBA
	mitochondrial large ribosomal subunit	GO:0005762	IDA
	mitochondrial large ribosomal subunit	GO:0005762	NAS

Experiment description of studies that identified MRPL27 in exosomes

Experiment ID

489

MISEV standards
✔ EM	Biophysical techniques
✔ Cd9\|Cd81\|Cd63\|Gapdh\|Sdcbp\|Lamp1\|Aqp1\|Rab5a\|Icam1\|Cd82\|Itga2b\|Tsg101\|Lamp2\|Rab35\|Flot1\|Flot2\|Cd151\|Rab5b\|Tfrc\|Uchl1	Enriched markers
✔ Canx	Negative markers
✔ NTA	Particle analysis

Identified molecule

Protein

Identification method

Mass spectrometry

PubMed ID

36408942

Organism

Rattus norvegicus

Experiment description

Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells

Authors

"Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."

Journal name

Proteomics

Publication year

2023

Sample

Bone marrow mesenchymal stem cells

Sample name

BMSC - Passage 6

Isolation/purification methods

Differential centrifugation
Filtration
Ultracentrifugation

Flotation density

Molecules identified in the study

Protein

Methods used in the study

Western blotting
Mass spectometry

Experiment ID

490

MISEV standards
✔ EM	Biophysical techniques
✔ Cd9\|Cd81\|Cd63\|Gapdh\|Sdcbp\|Lamp1\|Aqp1\|Rab5a\|Icam1\|Cd82\|Itga2b\|Tsg101\|Lamp2\|Rab35\|Flot1\|Flot2\|Cd151\|Rab5b\|Tfrc\|Uchl1	Enriched markers
✔ Canx	Negative markers
✔ NTA	Particle analysis

Identified molecule

Protein

Identification method

Mass spectrometry

PubMed ID

36408942

Organism

Rattus norvegicus

Experiment description

Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells

Authors

"Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."

Journal name

Proteomics

Publication year

2023

Sample

Bone marrow mesenchymal stem cells

Sample name

BMSC - Passage 7

Isolation/purification methods

Differential centrifugation
Filtration
Ultracentrifugation

Flotation density

Molecules identified in the study

Protein

Methods used in the study

Western blotting
Mass spectometry

Experiment ID

491

MISEV standards
✔ EM	Biophysical techniques
✔ Cd9\|Cd81\|Cd63\|Gapdh\|Sdcbp\|Lamp1\|Aqp1\|Rab5a\|Icam1\|Cd82\|Itga2b\|Tsg101\|Lamp2\|Rab35\|Flot1\|Flot2\|Cd151\|Rab5b\|Tfrc\|Uchl1	Enriched markers
✔ Canx	Negative markers
✔ NTA	Particle analysis

Identified molecule

Protein

Identification method

Mass spectrometry

PubMed ID

36408942

Organism

Rattus norvegicus

Experiment description

Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells

Authors

"Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."

Journal name

Proteomics

Publication year

2023

Sample

Bone marrow mesenchymal stem cells

Sample name

BMSC - Passage 8

Isolation/purification methods

Differential centrifugation
Filtration
Ultracentrifugation

Flotation density

Molecules identified in the study

Protein

Methods used in the study

Western blotting
Mass spectometry

Experiment ID

492

MISEV standards
✔ EM	Biophysical techniques
✔ Cd9\|Cd81\|Cd63\|Gapdh\|Sdcbp\|Lamp1\|Aqp1\|Rab5a\|Icam1\|Cd82\|Itga2b\|Tsg101\|Lamp2\|Rab35\|Flot1\|Flot2\|Cd151\|Rab5b\|Tfrc\|Uchl1	Enriched markers
✔ Canx	Negative markers
✔ NTA	Particle analysis

Identified molecule

Protein

Identification method

Mass spectrometry

PubMed ID

36408942

Organism

Rattus norvegicus

Experiment description

Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells

Authors

"Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."

Journal name

Proteomics

Publication year

2023

Sample

Bone marrow mesenchymal stem cells

Sample name

BMSC - Passage 9

Isolation/purification methods

Differential centrifugation
Filtration
Ultracentrifugation

Flotation density

Molecules identified in the study

Protein

Methods used in the study

Western blotting
Mass spectometry

Protein-protein interactions for MRPL27

Protein Interactor

ExoCarta ID

Identification method

PubMed

Species

ZNF185

7739

Affinity Capture-MS

Homo sapiens

UBE2H

7328

Affinity Capture-MS

Homo sapiens

SLIRP

81892

Proximity Label-MS

Homo sapiens

H1FNT

Affinity Capture-MS

Homo sapiens

MRPL48

Affinity Capture-MS

Homo sapiens

Smc1a

Affinity Capture-MS

Mus musculus

RPS3A

6189

Affinity Capture-MS

Homo sapiens

RBMS3

Affinity Capture-MS

Homo sapiens

COX15

1355

Proximity Label-MS

Homo sapiens

MRPL33

Affinity Capture-MS

Homo sapiens

MRPL34

Affinity Capture-MS

Homo sapiens

RPL13A

23521

Affinity Capture-MS

Homo sapiens

TRUB2

Proximity Label-MS

Homo sapiens

THOC2

57187

Affinity Capture-MS

Homo sapiens

MTG2

Proximity Label-MS

Homo sapiens

NGRN

Proximity Label-MS

Homo sapiens

RPS19

6223

Affinity Capture-MS

Homo sapiens

RPL10

6134

Affinity Capture-MS

Homo sapiens

CHCHD1

Proximity Label-MS

Homo sapiens

MRPL10

124995

Affinity Capture-MS		Homo sapiens
Affinity Capture-MS		Homo sapiens
Affinity Capture-MS		Homo sapiens

UBC

7316

Affinity Capture-MS

Homo sapiens

MRPL50

54534

Affinity Capture-MS		Homo sapiens
Affinity Capture-MS		Homo sapiens
Affinity Capture-MS		Homo sapiens
Affinity Capture-MS		Homo sapiens

MRPL52

Affinity Capture-MS		Homo sapiens
Affinity Capture-MS		Homo sapiens

MRPL49

740

Affinity Capture-MS		Homo sapiens
Affinity Capture-MS		Homo sapiens

MTRF1

Proximity Label-MS

Homo sapiens

APP

351

Reconstituted Complex

Homo sapiens

MSI1

Affinity Capture-MS

Homo sapiens

LRPPRC

10128

Proximity Label-MS

Homo sapiens

MRPL22

Affinity Capture-MS

Homo sapiens

MRPL20

55052

Affinity Capture-MS		Homo sapiens
Affinity Capture-MS		Homo sapiens

Elavl1

15568

Affinity Capture-MS

Mus musculus

PMPCA

23203

Proximity Label-MS

Homo sapiens

MRPL23

6150

Affinity Capture-MS

Homo sapiens

ACTL6B

Affinity Capture-MS

Homo sapiens

HINT2

84681

Proximity Label-MS

Homo sapiens

MRPL12

6182

Affinity Capture-MS		Homo sapiens
Affinity Capture-MS		Homo sapiens

Rfx5

Affinity Capture-MS

Mus musculus

DDX58

23586

Affinity Capture-RNA

Homo sapiens

RPS9

6203

Affinity Capture-MS

Homo sapiens

MYCN

Affinity Capture-MS

Homo sapiens

MRPS26

64949

Proximity Label-MS

Homo sapiens

MALSU1

Affinity Capture-MS

Homo sapiens

TRA2A

29896

Affinity Capture-MS

Homo sapiens

EXD2

Proximity Label-MS

Homo sapiens

MRPL39

54148

Affinity Capture-MS		Homo sapiens
Affinity Capture-MS		Homo sapiens
Affinity Capture-MS		Homo sapiens
Affinity Capture-MS		Homo sapiens

GNL3

26354

Affinity Capture-MS

Homo sapiens

Gspt1

14852

Affinity Capture-MS

Mus musculus

NHP2

55651

Affinity Capture-MS

Homo sapiens

PMPCB

9512

Proximity Label-MS

Homo sapiens

MRPL4

51073

Affinity Capture-MS		Homo sapiens
Affinity Capture-MS		Homo sapiens
Affinity Capture-MS		Homo sapiens
Affinity Capture-MS		Homo sapiens

VWA8

23078

Proximity Label-MS

Homo sapiens

MRPL47

57129

Affinity Capture-MS		Homo sapiens
Affinity Capture-MS		Homo sapiens
Affinity Capture-MS		Homo sapiens

RPL19

6143

Affinity Capture-MS

Homo sapiens

MTERF3

Proximity Label-MS

Homo sapiens

LIN28A

Affinity Capture-MS

Homo sapiens

MDH2

4191

Proximity Label-MS

Homo sapiens

TACO1

Proximity Label-MS

Homo sapiens

MRPS30

10884

Affinity Capture-MS		Homo sapiens
Affinity Capture-MS		Homo sapiens
Affinity Capture-MS		Homo sapiens
Affinity Capture-MS		Homo sapiens

MRPL13

Affinity Capture-MS		Homo sapiens
Affinity Capture-MS		Homo sapiens

MRPL57

Affinity Capture-MS

Homo sapiens

MRPL17

Affinity Capture-MS		Homo sapiens
Affinity Capture-MS		Homo sapiens

C6orf203

Proximity Label-MS

Homo sapiens

CBWD7

Affinity Capture-MS

Homo sapiens

RC3H2

Affinity Capture-MS

Homo sapiens

FHL3

2275

Two-hybrid		Homo sapiens
Two-hybrid		Homo sapiens

MRPL45

84311

Affinity Capture-MS		Homo sapiens
Affinity Capture-MS		Homo sapiens

MRPL30

Affinity Capture-MS

Homo sapiens

SRSF3

6428

Affinity Capture-MS

Homo sapiens

YBX2

51087

Affinity Capture-MS

Homo sapiens

TUFM

7284

Proximity Label-MS

Homo sapiens

MTIF3

Proximity Label-MS

Homo sapiens

ZNF22

Affinity Capture-MS

Homo sapiens

HSCB

150274

Proximity Label-MS

Homo sapiens

HIST1H2AM

8336

Affinity Capture-MS

Homo sapiens

PCCA

5095

Affinity Capture-MS

Homo sapiens

MRPL51

51258

Affinity Capture-MS		Homo sapiens
Affinity Capture-MS		Homo sapiens

CCDC109B

55013

Proximity Label-MS

Homo sapiens

GFM1

85476

Proximity Label-MS

Homo sapiens

CBX6

Affinity Capture-MS

Homo sapiens

KIF11

3832

Affinity Capture-MS

Homo sapiens

MRPL3

Affinity Capture-MS

Homo sapiens

MRPL11

65003

Proximity Label-MS		Homo sapiens
Affinity Capture-MS		Homo sapiens

MTRF1L

Proximity Label-MS

Homo sapiens

TMEM70

Proximity Label-MS

Homo sapiens

HIST1H2AB

8335

Affinity Capture-MS

Homo sapiens

PIPSL

266971

Affinity Capture-MS

Homo sapiens

MRPL41

64975

Affinity Capture-MS		Homo sapiens
Affinity Capture-MS		Homo sapiens

ZNF274

Affinity Capture-MS		Homo sapiens
Affinity Capture-MS		Homo sapiens

OXA1L

Affinity Capture-MS

Homo sapiens

Rab5c

19345

Affinity Capture-MS

Mus musculus

MRPL44

Affinity Capture-MS		Homo sapiens
Affinity Capture-MS		Homo sapiens

MRPL40

64976

Affinity Capture-MS

Homo sapiens

MRPL21

219927

Affinity Capture-MS		Homo sapiens
Affinity Capture-MS		Homo sapiens

MRRF

Proximity Label-MS

Homo sapiens

MRPS12

Proximity Label-MS

Homo sapiens

MRPL46

Affinity Capture-MS

Homo sapiens

MTIF2

4528

Proximity Label-MS

Homo sapiens

RPS2

6187

Affinity Capture-MS

Homo sapiens

MRPL24

Affinity Capture-MS

Homo sapiens

100

MRPL35

Affinity Capture-MS		Homo sapiens
Affinity Capture-MS		Homo sapiens

101

MRPL37

51253

Affinity Capture-MS		Homo sapiens
Affinity Capture-MS		Homo sapiens
Affinity Capture-MS		Homo sapiens

102

RPL35

11224

Affinity Capture-MS

Homo sapiens

103

MRPL9

65005

Affinity Capture-MS		Homo sapiens
Affinity Capture-MS		Homo sapiens
Affinity Capture-MS		Homo sapiens

104

MRPL16

Affinity Capture-MS

Homo sapiens

105

RPL37

6167

Affinity Capture-MS

Homo sapiens

106

FAHD1

81889

Affinity Capture-MS

Homo sapiens

107

GADD45GIP1

Affinity Capture-MS

Homo sapiens

108

HNRNPA1

3178

Affinity Capture-MS

Homo sapiens

109

KBTBD7

Affinity Capture-MS		Homo sapiens
Affinity Capture-MS		Homo sapiens

110

Max

Affinity Capture-MS

Mus musculus

111

MRPL15

29088

Affinity Capture-MS		Homo sapiens
Affinity Capture-MS		Homo sapiens

112

C12orf65

Proximity Label-MS

Homo sapiens

113

CDK2

1017

Affinity Capture-MS

Homo sapiens

114

PRR3

Affinity Capture-MS

Homo sapiens

115

MRPL19

9801

Affinity Capture-MS		Homo sapiens
Affinity Capture-MS		Homo sapiens

116

MTG1

Proximity Label-MS

Homo sapiens

117

MYC

Affinity Capture-MS

Homo sapiens

118

TSFM

10102

Proximity Label-MS

Homo sapiens

119

ACAD9

28976

Proximity Label-MS

Homo sapiens

120

SRSF10

10772

Affinity Capture-MS

Homo sapiens

121

RPL18

6141

Affinity Capture-MS

Homo sapiens

122

RPL7

6129

Affinity Capture-MS

Homo sapiens

123

MRPL38

Affinity Capture-MS		Homo sapiens
Affinity Capture-MS		Homo sapiens

124

HIST1H1T

3010

Affinity Capture-MS

Homo sapiens

125

METTL15

Proximity Label-MS

Homo sapiens

126

MCUR1

63933

Proximity Label-MS

Homo sapiens

127

RBMS2

5939

Affinity Capture-MS

Homo sapiens

128

FTL

2512

Affinity Capture-MS

Homo sapiens

129

RPL13

6137

Affinity Capture-MS		Homo sapiens
Affinity Capture-MS		Homo sapiens
Affinity Capture-MS		Homo sapiens

130

YBX1

4904

Affinity Capture-MS		Homo sapiens
Affinity Capture-MS		Homo sapiens

131

1431

Proximity Label-MS

Homo sapiens

132

MRPL42

Affinity Capture-MS		Homo sapiens
Affinity Capture-MS		Homo sapiens
Affinity Capture-MS		Homo sapiens

133

C17orf80

55028

Proximity Label-MS

Homo sapiens

134

RC3H1

149041

Affinity Capture-MS

Homo sapiens

135

AUH

549

Proximity Label-MS

Homo sapiens

136

PDGFB

Affinity Capture-MS

Homo sapiens

137

RPLP0

6175

Affinity Capture-MS

Homo sapiens

138

RPL6

6128

Affinity Capture-MS

Homo sapiens

139

ICT1

3396

Affinity Capture-MS		Homo sapiens
Proximity Label-MS		Homo sapiens
Affinity Capture-MS		Homo sapiens

140

FOXB1

Affinity Capture-MS

Homo sapiens

141

MRPL53

Affinity Capture-MS		Homo sapiens
Affinity Capture-MS		Homo sapiens

142

MRPL43

84545

Affinity Capture-MS		Homo sapiens
Affinity Capture-MS		Homo sapiens

143

ZZEF1

23140

Affinity Capture-MS

Homo sapiens

144

MRPL1

Affinity Capture-MS

Homo sapiens

145

MRPL54

Affinity Capture-MS

Homo sapiens

146

TRA2B

6434

Affinity Capture-MS

Homo sapiens

147

C21orf33

Proximity Label-MS

Homo sapiens

148

MRPL18

29074

Affinity Capture-MS		Homo sapiens
Affinity Capture-MS		Homo sapiens

149

SSBP1

6742

Proximity Label-MS		Homo sapiens
Affinity Capture-MS		Homo sapiens

150

NEIL1

Affinity Capture-MS

Homo sapiens

151

TBRG4

9238

Proximity Label-MS

Homo sapiens

152

THAP3

Affinity Capture-MS

Homo sapiens

153

RPL23AP32

56969

Affinity Capture-MS

Homo sapiens

154

RPS8

6202

Affinity Capture-MS		Homo sapiens
Affinity Capture-MS		Homo sapiens
Affinity Capture-MS		Homo sapiens

155

PCCB

5096

Affinity Capture-MS

Homo sapiens

156

C1QBP

708

Proximity Label-MS

Homo sapiens

157

HNRNPU

3192

Affinity Capture-MS		Homo sapiens
Affinity Capture-MS		Homo sapiens

158

MRPL2

51069

Affinity Capture-MS

Homo sapiens

159

MRPL55

Affinity Capture-MS

Homo sapiens

160

METTL17

Proximity Label-MS

Homo sapiens

161

MRPL28

10573

Affinity Capture-MS		Homo sapiens
Affinity Capture-MS		Homo sapiens
Affinity Capture-MS		Homo sapiens
Affinity Capture-MS		Homo sapiens

162

MRPS18A

Affinity Capture-MS		Homo sapiens
Affinity Capture-MS		Homo sapiens

163

MRPL32

64983

Affinity Capture-MS

Homo sapiens

164

HIST1H2AE

3012

Affinity Capture-MS

Homo sapiens

165

DDX28

Proximity Label-MS

Homo sapiens

166

RPL17

6139

Affinity Capture-MS

Homo sapiens

View the network

Pathways in which MRPL27 is involved

Pathway	Evidence	Source
Metabolism of proteins	TAS	Reactome
Metabolism of proteins	IEA	Reactome
Mitochondrial translation	TAS	Reactome
Mitochondrial translation	IEA	Reactome
Mitochondrial translation elongation	TAS	Reactome
Mitochondrial translation elongation	IEA	Reactome
Mitochondrial translation initiation	IEA	Reactome
Mitochondrial translation termination	TAS	Reactome
Translation	TAS	Reactome
Translation	IEA	Reactome

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