Gene description for MRPL32
Gene name mitochondrial ribosomal protein L32
Gene symbol MRPL32
Other names/aliases HSPC283
L32mt
MRP-L32
bMRP-59b
Species Homo sapiens
 Database cross references - MRPL32
ExoCarta ExoCarta_64983
Entrez Gene 64983
HGNC 14035
MIM 611839
UniProt Q9BYC8  
 MRPL32 identified in exosomes derived from the following tissue/cell type
Hepatocellular carcinoma cells 26054723    
Hepatocellular carcinoma cells 26054723    
 Gene ontology annotations for MRPL32
Molecular Function
    structural constituent of ribosome GO:0003735 NAS
    poly(A) RNA binding GO:0044822 IDA
Biological Process
    mitochondrial translational initiation GO:0070124 TAS
    translation GO:0006412 NAS
    mitochondrial translational elongation GO:0070125 TAS
    mitochondrial translation GO:0032543 TAS
    organelle organization GO:0006996 TAS
    mitochondrial translational termination GO:0070126 TAS
Subcellular Localization
    mitochondrial ribosome GO:0005761 NAS
    large ribosomal subunit GO:0015934 IEA
    mitochondrial inner membrane GO:0005743 TAS
 Experiment description of studies that identified MRPL32 in exosomes
1
Experiment ID 235
ISEV standards
✔
EM
EV Biophysical techniques
✔
TSG101|Alix|HSC70|GAPDH
EV Cytosolic markers
✘ EV Membrane markers
✔
HSP90B1
EV Negative markers
✔
qNano
EV Particle analysis
Identified molecule mRNA
Identification method RNA Sequencing
PubMed ID 26054723    
Organism Homo sapiens
Experiment description Hepatocellular carcinoma-derived exosomes promote motility of immortalized hepatocyte through transfer of oncogenic proteins and RNAs
Authors He M, Qin H, Poon TC, Sze SC, Ding X, Co NN, Ngai SM, Chan TF, Wong N
Journal name Carcinogenesis
Publication year 2015
Sample Hepatocellular carcinoma cells
Sample name HKCI-8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
RNA
Methods used in the study Western blotting
Mass spectrometry
RT-PCR
RNA Sequencing
2
Experiment ID 236
ISEV standards
✔
EM
EV Biophysical techniques
✔
TSG101|Alix|HSC70|GAPDH
EV Cytosolic markers
✘ EV Membrane markers
✔
HSP90B1
EV Negative markers
✔
qNano
EV Particle analysis
Identified molecule mRNA
Identification method RNA Sequencing
PubMed ID 26054723    
Organism Homo sapiens
Experiment description Hepatocellular carcinoma-derived exosomes promote motility of immortalized hepatocyte through transfer of oncogenic proteins and RNAs
Authors He M, Qin H, Poon TC, Sze SC, Ding X, Co NN, Ngai SM, Chan TF, Wong N
Journal name Carcinogenesis
Publication year 2015
Sample Hepatocellular carcinoma cells
Sample name MHCC97L
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
RNA
Methods used in the study Western blotting
Mass spectrometry
RT-PCR
RNA Sequencing
 Protein-protein interactions for MRPL32
  Protein Interactor ExoCarta ID Identification method PubMed Species
No interactions are found.



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