Gene description for MRPL32
Gene name mitochondrial ribosomal protein L32
Gene symbol MRPL32
Other names/aliases HSPC283
L32mt
MRP-L32
bMRP-59b
Species Homo sapiens
 Database cross references - MRPL32
ExoCarta ExoCarta_64983
Vesiclepedia VP_64983
Entrez Gene 64983
HGNC 14035
MIM 611839
UniProt Q9BYC8  
 MRPL32 identified in exosomes derived from the following tissue/cell type
Hepatocellular carcinoma cells 26054723    
Hepatocellular carcinoma cells 26054723    
 Gene ontology annotations for MRPL32
Molecular Function
    RNA binding GO:0003723 HDA
    structural constituent of ribosome GO:0003735 IBA
    structural constituent of ribosome GO:0003735 NAS
    protein binding GO:0005515 IPI
Biological Process
    translation GO:0006412 NAS
    mitochondrial translation GO:0032543 NAS
Subcellular Localization
    mitochondrion GO:0005739 HTP
    mitochondrion GO:0005739 IDA
    mitochondrial inner membrane GO:0005743 NAS
    mitochondrial inner membrane GO:0005743 TAS
    mitochondrial matrix GO:0005759 TAS
    mitochondrial ribosome GO:0005761 NAS
    mitochondrial large ribosomal subunit GO:0005762 IBA
    mitochondrial large ribosomal subunit GO:0005762 IDA
    mitochondrial large ribosomal subunit GO:0005762 NAS
 Experiment description of studies that identified MRPL32 in exosomes
1
Experiment ID 235
MISEV standards
EM
Biophysical techniques
TSG101|Alix|HSC70|GAPDH
Enriched markers
HSP90B1
Negative markers
qNano
Particle analysis
Identified molecule mRNA
Identification method RNA Sequencing
PubMed ID 26054723    
Organism Homo sapiens
Experiment description Hepatocellular carcinoma-derived exosomes promote motility of immortalized hepatocyte through transfer of oncogenic proteins and RNAs
Authors He M, Qin H, Poon TC, Sze SC, Ding X, Co NN, Ngai SM, Chan TF, Wong N
Journal name Carcinogenesis
Publication year 2015
Sample Hepatocellular carcinoma cells
Sample name HKCI-8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
RNA
Methods used in the study Western blotting
Mass spectrometry
RT-PCR
RNA Sequencing
2
Experiment ID 236
MISEV standards
EM
Biophysical techniques
TSG101|Alix|HSC70|GAPDH
Enriched markers
HSP90B1
Negative markers
qNano
Particle analysis
Identified molecule mRNA
Identification method RNA Sequencing
PubMed ID 26054723    
Organism Homo sapiens
Experiment description Hepatocellular carcinoma-derived exosomes promote motility of immortalized hepatocyte through transfer of oncogenic proteins and RNAs
Authors He M, Qin H, Poon TC, Sze SC, Ding X, Co NN, Ngai SM, Chan TF, Wong N
Journal name Carcinogenesis
Publication year 2015
Sample Hepatocellular carcinoma cells
Sample name MHCC97L
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
RNA
Methods used in the study Western blotting
Mass spectrometry
RT-PCR
RNA Sequencing
 Protein-protein interactions for MRPL32
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 HIST2H2BE 8349
Affinity Capture-MS Homo sapiens
2 H1FNT  
Affinity Capture-MS Homo sapiens
3 ZNF346  
Affinity Capture-MS Homo sapiens
4 RBMS3  
Affinity Capture-MS Homo sapiens
5 MRPL30  
Affinity Capture-MS Homo sapiens
6 MRPL17  
Cross-Linking-MS (XL-MS) Homo sapiens
7 RBMS2 5939
Affinity Capture-MS Homo sapiens
8 RPL13A 23521
Affinity Capture-MS Homo sapiens
9 TRUB2  
Proximity Label-MS Homo sapiens
10 NGRN  
Proximity Label-MS Homo sapiens
11 MRPL10 124995
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
12 MRPL27 51264
Affinity Capture-MS Homo sapiens
13 MRPL50 54534
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Co-fractionation Homo sapiens
14 MRPL52  
Affinity Capture-MS Homo sapiens
Co-fractionation Homo sapiens
15 PRDM14  
Two-hybrid Homo sapiens
16 IGF2BP3 10643
Affinity Capture-MS Homo sapiens
17 PMPCA 23203
Proximity Label-MS Homo sapiens
18 ZNF485  
Affinity Capture-MS Homo sapiens
19 MRPL23 6150
Affinity Capture-MS Homo sapiens
20 TIMM17B  
Co-fractionation Homo sapiens
21 MRPL12 6182
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
22 MRPL57  
Co-fractionation Homo sapiens
23 MRPS26 64949
Proximity Label-MS Homo sapiens
Co-fractionation Homo sapiens
24 MALSU1  
Affinity Capture-MS Homo sapiens
25 SRSF3 6428
Affinity Capture-MS Homo sapiens
26 MRPL39 54148
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Co-fractionation Homo sapiens
27 GNL3 26354
Affinity Capture-MS Homo sapiens
28 PMPCB 9512
Proximity Label-MS Homo sapiens
29 MRPL4 51073
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
30 MRPL47 57129
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
31 FAM120A 23196
Affinity Capture-MS Homo sapiens
32 LIN28A  
Affinity Capture-MS Homo sapiens
33 STOML2 30968
Co-fractionation Homo sapiens
34 MRPS30 10884
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
35 MRPL13  
Affinity Capture-MS Homo sapiens
36 MPV17 4358
Co-fractionation Homo sapiens
37 SCFD1 23256
Co-fractionation Homo sapiens
38 C6orf203  
Affinity Capture-MS Homo sapiens
Proximity Label-MS Homo sapiens
39 LIN28B  
Affinity Capture-MS Homo sapiens
40 MRPL45 84311
Co-fractionation Homo sapiens
41 TRA2A 29896
Affinity Capture-MS Homo sapiens
42 YBX2 51087
Affinity Capture-MS Homo sapiens
43 SRSF6 6431
Affinity Capture-MS Homo sapiens
44 RPL35 11224
Affinity Capture-MS Homo sapiens
45 FUBP1 8880
Co-fractionation Homo sapiens
46 MRPL51 51258
Affinity Capture-MS Homo sapiens
47 REXO4  
Affinity Capture-MS Homo sapiens
48 RPL27A 6157
Affinity Capture-MS Homo sapiens
49 MRPL11 65003
Co-fractionation Homo sapiens
Proximity Label-MS Homo sapiens
50 MRPL2 51069
Affinity Capture-MS Homo sapiens
Co-fractionation Homo sapiens
51 MRPL41 64975
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
52 HNRNPDL 9987
Affinity Capture-MS Homo sapiens
53 MRPL44  
Co-fractionation Homo sapiens
54 MRPL40 64976
Co-fractionation Homo sapiens
55 MRPL21 219927
Co-fractionation Homo sapiens
56 MRRF  
Proximity Label-MS Homo sapiens
57 SRSF7 6432
Affinity Capture-MS Homo sapiens
58 MTIF2 4528
Proximity Label-MS Homo sapiens
59 MRPL37 51253
Affinity Capture-MS Homo sapiens
Co-fractionation Homo sapiens
Co-fractionation Homo sapiens
60 SRSF4 6429
Affinity Capture-MS Homo sapiens
61 MRPL9 65005
Co-fractionation Homo sapiens
Affinity Capture-MS Homo sapiens
Co-fractionation Homo sapiens
62 SRSF5 6430
Affinity Capture-MS Homo sapiens
63 RPL3 6122
Affinity Capture-MS Homo sapiens
64 GADD45GIP1  
Affinity Capture-MS Homo sapiens
65 HNRNPA1 3178
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
66 MRPL15 29088
Co-fractionation Homo sapiens
67 C12orf65  
Proximity Label-MS Homo sapiens
68 MYCN  
Affinity Capture-MS Homo sapiens
69 PRR3  
Affinity Capture-MS Homo sapiens
70 MYC  
Affinity Capture-MS Homo sapiens
71 RPL18 6141
Affinity Capture-MS Homo sapiens
72 MRPL38  
Co-fractionation Homo sapiens
73 TSR1 55720
Co-fractionation Homo sapiens
74 RPL26 6154
Affinity Capture-MS Homo sapiens
75 ZC3H3  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
76 MRPL18 29074
Affinity Capture-MS Homo sapiens
Co-fractionation Homo sapiens
77 RPL19 6143
Affinity Capture-MS Homo sapiens
78 ZNF574  
Affinity Capture-MS Homo sapiens
79 RPL13 6137
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
80 YBX1 4904
Affinity Capture-MS Homo sapiens
81 RPL37 6167
Affinity Capture-MS Homo sapiens
82 MRPL42  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Co-fractionation Homo sapiens
83 TSFM 10102
Proximity Label-MS Homo sapiens
84 AUH 549
Proximity Label-MS Homo sapiens
85 ILF3 3609
Co-fractionation Homo sapiens
86 RRS1 23212
Affinity Capture-MS Homo sapiens
87 RPL6 6128
Affinity Capture-MS Homo sapiens
88 ICT1 3396
Affinity Capture-MS Homo sapiens
Proximity Label-MS Homo sapiens
89 MRPS28  
Co-fractionation Homo sapiens
90 MRPL53  
Affinity Capture-MS Homo sapiens
91 MRPL1  
Affinity Capture-MS Homo sapiens
92 ERGIC1 57222
Co-fractionation Homo sapiens
93 C21orf33  
Proximity Label-MS Homo sapiens
94 NEIL1  
Affinity Capture-MS Homo sapiens
95 MRPS9 64965
Co-fractionation Homo sapiens
96 MRPL46  
Affinity Capture-MS Homo sapiens
97 RPL23AP32 56969
Affinity Capture-MS Homo sapiens
98 RPS8 6202
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
99 RPL23A 6147
Affinity Capture-MS Homo sapiens
100 HNRNPU 3192
Affinity Capture-MS Homo sapiens
101 MRPL55  
Co-fractionation Homo sapiens
102 METTL17  
Proximity Label-MS Homo sapiens
103 MRPL28 10573
Co-fractionation Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
104 ZNF467  
Affinity Capture-MS Homo sapiens
105 MRPS18A  
Affinity Capture-MS Homo sapiens
106 DDX28  
Co-fractionation Homo sapiens
107 RPL17 6139
Affinity Capture-MS Homo sapiens
View the network image/svg+xml



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