Gene ontology annotations for ERGIC1 |
|
Experiment description of studies that identified ERGIC1 in exosomes |
1 |
Experiment ID |
489 |
MISEV standards |
✔
EM
|
Biophysical techniques |
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
|
Enriched markers |
✔
Canx
|
Negative markers |
✔
NTA
|
Particle analysis
|
|
Identified molecule |
Protein
|
Identification method |
Mass spectrometry
|
PubMed ID |
36408942
|
Organism |
Rattus norvegicus |
Experiment description |
Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells |
Authors |
"Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ." |
Journal name |
Proteomics
|
Publication year |
2023 |
Sample |
Bone marrow mesenchymal stem cells |
Sample name |
BMSC - Passage 6 |
Isolation/purification methods |
Differential centrifugation Filtration Ultracentrifugation |
Flotation density |
-
|
Molecules identified in the study |
Protein |
Methods used in the study |
Western blotting Mass spectometry |
|
|
2 |
Experiment ID |
490 |
MISEV standards |
✔
EM
|
Biophysical techniques |
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
|
Enriched markers |
✔
Canx
|
Negative markers |
✔
NTA
|
Particle analysis
|
|
Identified molecule |
Protein
|
Identification method |
Mass spectrometry
|
PubMed ID |
36408942
|
Organism |
Rattus norvegicus |
Experiment description |
Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells |
Authors |
"Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ." |
Journal name |
Proteomics
|
Publication year |
2023 |
Sample |
Bone marrow mesenchymal stem cells |
Sample name |
BMSC - Passage 7 |
Isolation/purification methods |
Differential centrifugation Filtration Ultracentrifugation |
Flotation density |
-
|
Molecules identified in the study |
Protein |
Methods used in the study |
Western blotting Mass spectometry |
|
|
3 |
Experiment ID |
491 |
MISEV standards |
✔
EM
|
Biophysical techniques |
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
|
Enriched markers |
✔
Canx
|
Negative markers |
✔
NTA
|
Particle analysis
|
|
Identified molecule |
Protein
|
Identification method |
Mass spectrometry
|
PubMed ID |
36408942
|
Organism |
Rattus norvegicus |
Experiment description |
Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells |
Authors |
"Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ." |
Journal name |
Proteomics
|
Publication year |
2023 |
Sample |
Bone marrow mesenchymal stem cells |
Sample name |
BMSC - Passage 8 |
Isolation/purification methods |
Differential centrifugation Filtration Ultracentrifugation |
Flotation density |
-
|
Molecules identified in the study |
Protein |
Methods used in the study |
Western blotting Mass spectometry |
|
|
4 |
Experiment ID |
492 |
MISEV standards |
✔
EM
|
Biophysical techniques |
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
|
Enriched markers |
✔
Canx
|
Negative markers |
✔
NTA
|
Particle analysis
|
|
Identified molecule |
Protein
|
Identification method |
Mass spectrometry
|
PubMed ID |
36408942
|
Organism |
Rattus norvegicus |
Experiment description |
Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells |
Authors |
"Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ." |
Journal name |
Proteomics
|
Publication year |
2023 |
Sample |
Bone marrow mesenchymal stem cells |
Sample name |
BMSC - Passage 9 |
Isolation/purification methods |
Differential centrifugation Filtration Ultracentrifugation |
Flotation density |
-
|
Molecules identified in the study |
Protein |
Methods used in the study |
Western blotting Mass spectometry |
|
|
5 |
Experiment ID |
426 |
MISEV standards |
✘
|
Biophysical techniques |
✔
SDCBP|FLOT1|CD81|CD9|CD151|FLOT2|TSG101|LAMP1|TFRC|RAB5A|RAB35|GAPDH|UCHL1|ICAM1
|
Enriched markers |
✔
CANX
|
Negative markers |
✘
|
Particle analysis
|
|
Identified molecule |
Protein
|
Identification method |
Mass spectrometry
|
PubMed ID |
34108659
|
Organism |
Homo sapiens |
Experiment description |
Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker |
Authors |
"Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R." |
Journal name |
Nat Cell Biol
|
Publication year |
2021 |
Sample |
Breast cancer cells |
Sample name |
MDA-MB-231 - Exo-rich fractions 7-10 pooled |
Isolation/purification methods |
Differential centrifugation Filtration Ultracentrifugation Size exclusion chromatography |
Flotation density |
-
|
Molecules identified in the study |
Protein |
Methods used in the study |
Western blotting Mass spectrometry |
|
|
6 |
Experiment ID |
427 |
MISEV standards |
✘
|
Biophysical techniques |
✔
CD81|SDCBP|FLOT1|CD9|CD151|FLOT2|TSG101|LAMP1|TFRC|RAB5A|RAB35|GAPDH|UCHL1|ICAM1
|
Enriched markers |
✔
CANX
|
Negative markers |
✘
|
Particle analysis
|
|
Identified molecule |
Protein
|
Identification method |
Mass spectrometry
|
PubMed ID |
34108659
|
Organism |
Homo sapiens |
Experiment description |
Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker |
Authors |
"Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R." |
Journal name |
Nat Cell Biol
|
Publication year |
2021 |
Sample |
Breast cancer cells |
Sample name |
MDA-MB-231 - Exo-rich fractions 1-6 pooled |
Isolation/purification methods |
Differential centrifugation Filtration Ultracentrifugation OptiPrep density gradient centrifugation |
Flotation density |
-
|
Molecules identified in the study |
Protein |
Methods used in the study |
Mass spectrometry |
|
|
7 |
Experiment ID |
419 |
MISEV standards |
✘
|
Biophysical techniques |
✔
SDCBP|FLOT1|CD81|CD9|CD151|FLOT2|TSG101|LAMP1|TFRC|RAB5A|RAB35|GAPDH|UCHL1|ICAM1
|
Enriched markers |
✔
CANX
|
Negative markers |
✘
|
Particle analysis
|
|
Identified molecule |
Protein
|
Identification method |
Mass spectrometry
|
PubMed ID |
34108659
|
Organism |
Homo sapiens |
Experiment description |
Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker |
Authors |
"Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R." |
Journal name |
Nat Cell Biol
|
Publication year |
2021 |
Sample |
Embryonic kidney cells |
Sample name |
HEK293T - Exo-rich fractions 7-10 pooled |
Isolation/purification methods |
Differential centrifugation Filtration Ultracentrifugation Size exclusion chromatography |
Flotation density |
-
|
Molecules identified in the study |
Protein |
Methods used in the study |
Western blotting Mass spectrometry |
|
|
8 |
Experiment ID |
419 |
MISEV standards |
✘
|
Biophysical techniques |
✔
SDCBP|FLOT1|CD81|CD9|CD151|FLOT2|TSG101|LAMP1|TFRC|RAB5A|RAB35|GAPDH|UCHL1|ICAM1
|
Enriched markers |
✔
CANX
|
Negative markers |
✘
|
Particle analysis
|
|
Identified molecule |
Protein
|
Identification method |
Mass spectrometry
|
PubMed ID |
34108659
|
Organism |
Homo sapiens |
Experiment description |
Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker |
Authors |
"Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R." |
Journal name |
Nat Cell Biol
|
Publication year |
2021 |
Sample |
Embryonic kidney cells |
Sample name |
HEK293T - Exo-rich fractions 7-10 pooled |
Isolation/purification methods |
Differential centrifugation Filtration Ultracentrifugation Size exclusion chromatography |
Flotation density |
-
|
Molecules identified in the study |
Protein |
Methods used in the study |
Western blotting Mass spectrometry |
|
|
9 |
Experiment ID |
420 |
MISEV standards |
✘
|
Biophysical techniques |
✔
CD81|SDCBP|FLOT1|CD9|CD151|FLOT2|TSG101|LAMP1|TFRC|RAB5A|RAB35|GAPDH|UCHL1|ICAM1
|
Enriched markers |
✔
CANX
|
Negative markers |
✘
|
Particle analysis
|
|
Identified molecule |
Protein
|
Identification method |
Mass spectrometry
|
PubMed ID |
34108659
|
Organism |
Homo sapiens |
Experiment description |
Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker |
Authors |
"Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R." |
Journal name |
Nat Cell Biol
|
Publication year |
2021 |
Sample |
Embryonic kidney cells |
Sample name |
HEK293T - Exo-rich fractions 1-6 pooled |
Isolation/purification methods |
Differential centrifugation Filtration Ultracentrifugation OptiPrep density gradient centrifugation |
Flotation density |
-
|
Molecules identified in the study |
Protein |
Methods used in the study |
Mass spectrometry |
|
|
10 |
Experiment ID |
420 |
MISEV standards |
✘
|
Biophysical techniques |
✔
CD81|SDCBP|FLOT1|CD9|CD151|FLOT2|TSG101|LAMP1|TFRC|RAB5A|RAB35|GAPDH|UCHL1|ICAM1
|
Enriched markers |
✔
CANX
|
Negative markers |
✘
|
Particle analysis
|
|
Identified molecule |
Protein
|
Identification method |
Mass spectrometry
|
PubMed ID |
34108659
|
Organism |
Homo sapiens |
Experiment description |
Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker |
Authors |
"Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R." |
Journal name |
Nat Cell Biol
|
Publication year |
2021 |
Sample |
Embryonic kidney cells |
Sample name |
HEK293T - Exo-rich fractions 1-6 pooled |
Isolation/purification methods |
Differential centrifugation Filtration Ultracentrifugation OptiPrep density gradient centrifugation |
Flotation density |
-
|
Molecules identified in the study |
Protein |
Methods used in the study |
Mass spectrometry |
|
|
11 |
Experiment ID |
363 |
MISEV standards |
✘
|
Biophysical techniques |
✔
TSG101|GAPDH|AQP1|CD151|CD81|CD82|CD9|EPCAM|FLOT1|FLOT2|ICAM1|ITGA2B|LAMP2|RAB35|RAB5A|RAB5B|SDCBP|TFRC|UCHL1
|
Enriched markers |
✔
DCLK1
|
Negative markers |
✔
NTA
|
Particle analysis
|
|
Identified molecule |
Protein
|
Identification method |
Mass spectrometry
|
PubMed ID |
33991177
|
Organism |
Homo sapiens |
Experiment description |
Cancer stem cell marker DCLK1 reprograms small extracellular vesicles toward migratory phenotype in gastric cancer cells |
Authors |
"Carli ALE, Afshar-Sterle S, Rai A, Fang H, O'Keefe R, Tse J, Ferguson FM, Gray NS, Ernst M, Greening DW, Buchert M." |
Journal name |
Proteomics
|
Publication year |
2021 |
Sample |
Gastric cancer cells |
Sample name |
MKN1 - 100K pellet |
Isolation/purification methods |
Differential centrifugation Ultracentrifugation |
Flotation density |
-
|
Molecules identified in the study |
Protein |
Methods used in the study |
Western blotting Mass spectrometry |
|
|
12 |
Experiment ID |
364 |
MISEV standards |
✘
|
Biophysical techniques |
✔
TSG101|GAPDH|AQP1|CD151|CD81|CD82|CD9|EPCAM|FLOT1|FLOT2|ICAM1|ITGA2B|LAMP2|RAB35|RAB5A|RAB5B|SDCBP|TFRC|UCHL1
|
Enriched markers |
✔
DCLK1
|
Negative markers |
✔
NTA
|
Particle analysis
|
|
Identified molecule |
Protein
|
Identification method |
Mass spectrometry
|
PubMed ID |
33991177
|
Organism |
Homo sapiens |
Experiment description |
Cancer stem cell marker DCLK1 reprograms small extracellular vesicles toward migratory phenotype in gastric cancer cells |
Authors |
"Carli ALE, Afshar-Sterle S, Rai A, Fang H, O'Keefe R, Tse J, Ferguson FM, Gray NS, Ernst M, Greening DW, Buchert M." |
Journal name |
Proteomics
|
Publication year |
2021 |
Sample |
Gastric cancer cells |
Sample name |
MKN1 - 100K pellet |
Isolation/purification methods |
Differential centrifugation Ultracentrifugation |
Flotation density |
-
|
Molecules identified in the study |
Protein |
Methods used in the study |
Western blotting Mass spectrometry |
|
|
13 |
Experiment ID |
365 |
MISEV standards |
✘
|
Biophysical techniques |
✔
TSG101|GAPDH|AQP1|CD151|CD81|CD82|CD9|EPCAM|FLOT1|FLOT2|ICAM1|ITGA2B|LAMP2|RAB35|RAB5A|RAB5B|SDCBP|TFRC|UCHL1
|
Enriched markers |
✔
DCLK1
|
Negative markers |
✔
NTA
|
Particle analysis
|
|
Identified molecule |
Protein
|
Identification method |
Mass spectrometry
|
PubMed ID |
33991177
|
Organism |
Homo sapiens |
Experiment description |
Cancer stem cell marker DCLK1 reprograms small extracellular vesicles toward migratory phenotype in gastric cancer cells |
Authors |
"Carli ALE, Afshar-Sterle S, Rai A, Fang H, O'Keefe R, Tse J, Ferguson FM, Gray NS, Ernst M, Greening DW, Buchert M." |
Journal name |
Proteomics
|
Publication year |
2021 |
Sample |
Gastric cancer cells |
Sample name |
MKN1 - 100K pellet |
Isolation/purification methods |
Differential centrifugation Ultracentrifugation |
Flotation density |
-
|
Molecules identified in the study |
Protein |
Methods used in the study |
Western blotting Mass spectrometry |
|
|
14 |
Experiment ID |
235 |
MISEV standards |
✔
EM
|
Biophysical techniques |
✔
TSG101|Alix|HSC70|GAPDH
|
Enriched markers |
✔
HSP90B1
|
Negative markers |
✔
qNano
|
Particle analysis
|
|
Identified molecule |
mRNA
|
Identification method |
RNA Sequencing
|
PubMed ID |
26054723
|
Organism |
Homo sapiens |
Experiment description |
Hepatocellular carcinoma-derived exosomes promote motility of immortalized hepatocyte through transfer of oncogenic proteins and RNAs |
Authors |
"He M, Qin H, Poon TC, Sze SC, Ding X, Co NN, Ngai SM, Chan TF, Wong N" |
Journal name |
Carcinogenesis
|
Publication year |
2015 |
Sample |
Hepatocellular carcinoma cells |
Sample name |
HKCI-8 |
Isolation/purification methods |
Differential centrifugation Filtration Ultracentrifugation Sucrose density gradient |
Flotation density |
1.13-1.19 g/mL
|
Molecules identified in the study |
Protein RNA |
Methods used in the study |
Western blotting Mass spectrometry RT-PCR RNA Sequencing |
|
|
15 |
Experiment ID |
236 |
MISEV standards |
✔
EM
|
Biophysical techniques |
✔
TSG101|Alix|HSC70|GAPDH
|
Enriched markers |
✔
HSP90B1
|
Negative markers |
✔
qNano
|
Particle analysis
|
|
Identified molecule |
mRNA
|
Identification method |
RNA Sequencing
|
PubMed ID |
26054723
|
Organism |
Homo sapiens |
Experiment description |
Hepatocellular carcinoma-derived exosomes promote motility of immortalized hepatocyte through transfer of oncogenic proteins and RNAs |
Authors |
"He M, Qin H, Poon TC, Sze SC, Ding X, Co NN, Ngai SM, Chan TF, Wong N" |
Journal name |
Carcinogenesis
|
Publication year |
2015 |
Sample |
Hepatocellular carcinoma cells |
Sample name |
MHCC97L |
Isolation/purification methods |
Differential centrifugation Filtration Ultracentrifugation Sucrose density gradient |
Flotation density |
1.13-1.19 g/mL
|
Molecules identified in the study |
Protein RNA |
Methods used in the study |
Western blotting Mass spectrometry RT-PCR RNA Sequencing |
|
|
16 |
Experiment ID |
488 |
MISEV standards |
✔
EM
|
Biophysical techniques |
✔
CD9|CD81|CD63|GAPDH|SDCBP|LAMP1|TFRC|UCHL1|FLOT2|LAMP2|FLOT1|ICAM1|RAB5B|CD151|RAB35|TSG101|RAB5A|CD82
|
Enriched markers |
✔
CANX
|
Negative markers |
✔
NTA
|
Particle analysis
|
|
Identified molecule |
Protein
|
Identification method |
Mass spectrometry
|
PubMed ID |
36408942
|
Organism |
Homo sapiens |
Experiment description |
Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells |
Authors |
"Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ." |
Journal name |
Proteomics
|
Publication year |
2023 |
Sample |
Mesenchymal stem cells |
Sample name |
UCMSC |
Isolation/purification methods |
Differential centrifugation Filtration Ultracentrifugation |
Flotation density |
-
|
Molecules identified in the study |
Protein |
Methods used in the study |
Western blotting Mass spectometry |
|
|
17 |
Experiment ID |
211 |
MISEV standards |
✔
EM
|
Biophysical techniques |
✔
TSG101|Alix|EpCAM|TFRC
|
Enriched markers |
✔
cytochrome c|GOLGA2
|
Negative markers |
✘
|
Particle analysis
|
|
Identified molecule |
protein
|
Identification method |
Mass spectrometry
|
PubMed ID |
23333927
|
Organism |
Homo sapiens |
Experiment description |
Characterization and proteomic analysis of ovarian cancer-derived exosomes. |
Authors |
"Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K." |
Journal name |
J Proteomics
|
Publication year |
2013 |
Sample |
Ovarian cancer cells |
Sample name |
IGROV1 |
Isolation/purification methods |
Differential centrifugation Ultracentrifugation Sucrose density gradient |
Flotation density |
1.09-1.15 g/mL
|
Molecules identified in the study |
Protein |
Methods used in the study |
Mass spectrometry |
|
|
18 |
Experiment ID |
212 |
MISEV standards |
✔
CEM
|
Biophysical techniques |
✔
TSG101|Alix|EpCAM|TFRC
|
Enriched markers |
✔
Cytochrome C|GOLGA2
|
Negative markers |
✘
|
Particle analysis
|
|
Identified molecule |
protein
|
Identification method |
Mass spectrometry
|
PubMed ID |
23333927
|
Organism |
Homo sapiens |
Experiment description |
Characterization and proteomic analysis of ovarian cancer-derived exosomes. |
Authors |
"Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K." |
Journal name |
J Proteomics
|
Publication year |
2013 |
Sample |
Ovarian cancer cells |
Sample name |
OVCAR-3 |
Isolation/purification methods |
Differential centrifugation Ultracentrifugation Sucrose density gradient |
Flotation density |
1.09-1.15 g/mL
|
Molecules identified in the study |
Protein |
Methods used in the study |
Mass spectrometry |
|
|
19 |
Experiment ID |
434 |
MISEV standards |
✘
|
Biophysical techniques |
✔
SDCBP|FLOT1|CD81|CD9|CD151|FLOT2|TSG101|LAMP1|TFRC|RAB5A|RAB35|GAPDH|UCHL1|ICAM1
|
Enriched markers |
✔
CANX
|
Negative markers |
✘
|
Particle analysis
|
|
Identified molecule |
Protein
|
Identification method |
Mass spectrometry
|
PubMed ID |
34108659
|
Organism |
Homo sapiens |
Experiment description |
Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker |
Authors |
"Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R." |
Journal name |
Nat Cell Biol
|
Publication year |
2021 |
Sample |
Pancreatic cancer cells |
Sample name |
PANC-1 - Exo-rich fractions 7-10 pooled |
Isolation/purification methods |
Differential centrifugation Filtration Ultracentrifugation Size exclusion chromatography |
Flotation density |
-
|
Molecules identified in the study |
Protein |
Methods used in the study |
Western blotting Mass spectrometry |
|
|
20 |
Experiment ID |
435 |
MISEV standards |
✘
|
Biophysical techniques |
✔
CD81|SDCBP|FLOT1|CD9|CD151|FLOT2|TSG101|LAMP1|TFRC|RAB5A|RAB35|GAPDH|UCHL1|ICAM1
|
Enriched markers |
✔
CANX
|
Negative markers |
✘
|
Particle analysis
|
|
Identified molecule |
Protein
|
Identification method |
Mass spectrometry
|
PubMed ID |
34108659
|
Organism |
Homo sapiens |
Experiment description |
Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker |
Authors |
"Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R." |
Journal name |
Nat Cell Biol
|
Publication year |
2021 |
Sample |
Pancreatic cancer cells |
Sample name |
PANC-1 - Exo-rich fractions 1-6 pooled |
Isolation/purification methods |
Differential centrifugation Filtration Ultracentrifugation OptiPrep density gradient centrifugation |
Flotation density |
-
|
Molecules identified in the study |
Protein |
Methods used in the study |
Mass spectrometry |
|
|
21 |
Experiment ID |
834 |
MISEV standards |
✔
EM
|
Biophysical techniques |
✔
LAMP2|CD63|FLOT1|ITGA2B|ICAM1|CD9|CD151|TFRC|RAB5A|GAPDH|AQP1|TSG101
|
Enriched markers |
✔
CANX
|
Negative markers |
✔
NTA
|
Particle analysis
|
|
Identified molecule |
Protein
|
Identification method |
Mass spectrometry
|
PubMed ID |
35333565
|
Organism |
Homo sapiens |
Experiment description |
LAMP2A regulates the loading of proteins into exosomes |
Authors |
"Ferreira JV, da Rosa Soares A, Ramalho J, Máximo Carvalho C, Cardoso MH, Pintado P, Carvalho AS, Beck HC, Matthiesen R, Zuzarte M, Girão H, van Niel G, Pereira P" |
Journal name |
Sci Adv
|
Publication year |
2022 |
Sample |
Retinal pigment epithelial cells |
Sample name |
ARPE-19 |
Isolation/purification methods |
Differential centrifugation Filtration Ultracentrifugation |
Flotation density |
-
|
Molecules identified in the study |
Protein |
Methods used in the study |
Western blotting Mass spectrometry |
|
|
22 |
Experiment ID |
835 |
MISEV standards |
✔
EM
|
Biophysical techniques |
✔
CD63|FLOT1|ITGA2B|ICAM1|CD9|CD151|TFRC|RAB5A|GAPDH|AQP1|TSG101
|
Enriched markers |
✔
CANX
|
Negative markers |
✔
NTA
|
Particle analysis
|
|
Identified molecule |
Protein
|
Identification method |
Mass spectrometry
|
PubMed ID |
35333565
|
Organism |
Homo sapiens |
Experiment description |
LAMP2A regulates the loading of proteins into exosomes |
Authors |
"Ferreira JV, da Rosa Soares A, Ramalho J, Máximo Carvalho C, Cardoso MH, Pintado P, Carvalho AS, Beck HC, Matthiesen R, Zuzarte M, Girão H, van Niel G, Pereira P" |
Journal name |
Sci Adv
|
Publication year |
2022 |
Sample |
Retinal pigment epithelial cells |
Sample name |
ARPE-19 |
Isolation/purification methods |
Differential centrifugation Filtration Ultracentrifugation |
Flotation density |
-
|
Molecules identified in the study |
Protein |
Methods used in the study |
Western blotting Mass spectrometry |
|
|
Protein-protein interactions for ERGIC1 |
|
Protein Interactor |
ExoCarta ID |
Identification method |
PubMed |
Species |
1 |
SEC22B |
9554 |
Proximity Label-MS |
 |
Homo sapiens |
|
2 |
HNRNPH1 |
3187 |
Affinity Capture-RNA |
 |
Homo sapiens |
|
3 |
ITGB1 |
3688 |
Proximity Label-MS |
 |
Homo sapiens |
|
4 |
Myh3 |
|
Affinity Capture-MS |
 |
Mus musculus |
|
5 |
WDR6 |
11180 |
Proximity Label-MS |
 |
Homo sapiens |
|
6 |
ANKLE2 |
23141 |
Proximity Label-MS |
 |
Homo sapiens |
|
7 |
SLC1A5 |
6510 |
Proximity Label-MS |
 |
Homo sapiens |
|
8 |
AGPAT1 |
10554 |
Proximity Label-MS |
 |
Homo sapiens |
|
9 |
OCLN |
100506658 |
Proximity Label-MS |
 |
Homo sapiens |
|
10 |
SNAP23 |
8773 |
Proximity Label-MS |
 |
Homo sapiens |
|
11 |
PRKCD |
5580 |
Proximity Label-MS |
 |
Homo sapiens |
|
12 |
VAPA |
9218 |
Proximity Label-MS |
 |
Homo sapiens |
|
13 |
DHRS7 |
51635 |
Proximity Label-MS |
 |
Homo sapiens |
|
14 |
LLGL1 |
3996 |
Proximity Label-MS |
 |
Homo sapiens |
|
15 |
SRPRB |
58477 |
Proximity Label-MS |
 |
Homo sapiens |
|
16 |
ARL6IP5 |
10550 |
Proximity Label-MS |
 |
Homo sapiens |
|
17 |
RPA2 |
6118 |
Affinity Capture-MS |
 |
Homo sapiens |
|
18 |
JPH1 |
56704 |
Proximity Label-MS |
 |
Homo sapiens |
|
19 |
SLC30A1 |
7779 |
Proximity Label-MS |
 |
Homo sapiens |
|
20 |
ARFGAP3 |
26286 |
Proximity Label-MS |
 |
Homo sapiens |
|
21 |
SLC29A1 |
2030 |
Proximity Label-MS |
 |
Homo sapiens |
|
22 |
SLC2A1 |
6513 |
Proximity Label-MS |
 |
Homo sapiens |
|
23 |
Trp53bp1 |
|
Affinity Capture-MS |
 |
Mus musculus |
|
24 |
ARCN1 |
372 |
Proximity Label-MS |
 |
Homo sapiens |
|
25 |
SYNE2 |
23224 |
Proximity Label-MS |
 |
Homo sapiens |
|
26 |
GOSR2 |
9570 |
Proximity Label-MS |
 |
Homo sapiens |
|
27 |
PTPN1 |
5770 |
Proximity Label-MS |
 |
Homo sapiens |
|
28 |
SNX6 |
58533 |
Proximity Label-MS |
 |
Homo sapiens |
|
29 |
COPA |
1314 |
Affinity Capture-MS |
 |
Homo sapiens |
|
30 |
ARHGAP1 |
392 |
Proximity Label-MS |
 |
Homo sapiens |
|
31 |
FFAR1 |
|
Affinity Capture-MS |
 |
Homo sapiens |
|
32 |
PTPN2 |
5771 |
Proximity Label-MS |
 |
Homo sapiens |
|
33 |
KLK2 |
|
Affinity Capture-MS |
 |
Homo sapiens |
|
34 |
MFAP5 |
|
Affinity Capture-MS |
 |
Homo sapiens |
|
35 |
PDE2A |
|
Affinity Capture-MS |
 |
Homo sapiens |
|
36 |
TRPM7 |
54822 |
Proximity Label-MS |
 |
Homo sapiens |
|
37 |
KIAA1429 |
25962 |
Affinity Capture-MS |
 |
Homo sapiens |
|
38 |
TEX264 |
51368 |
Proximity Label-MS |
 |
Homo sapiens |
|
39 |
INA |
9118 |
Affinity Capture-MS |
 |
Homo sapiens |
Affinity Capture-MS |
 |
Homo sapiens |
Affinity Capture-MS |
 |
Homo sapiens |
|
40 |
WDR41 |
|
Proximity Label-MS |
 |
Homo sapiens |
|
41 |
DGKH |
160851 |
Proximity Label-MS |
 |
Homo sapiens |
|
42 |
HELZ |
9931 |
Proximity Label-MS |
 |
Homo sapiens |
|
43 |
RAB40C |
57799 |
Affinity Capture-MS |
 |
Homo sapiens |
|
44 |
COPB2 |
9276 |
Affinity Capture-MS |
 |
Homo sapiens |
|
45 |
TMED2 |
10959 |
Co-fractionation |
 |
Homo sapiens |
|
46 |
CISD2 |
493856 |
Proximity Label-MS |
 |
Homo sapiens |
|
47 |
TOMM7 |
54543 |
Co-fractionation |
 |
Homo sapiens |
|
48 |
RER1 |
11079 |
Proximity Label-MS |
 |
Homo sapiens |
|
49 |
PGRMC2 |
10424 |
Proximity Label-MS |
 |
Homo sapiens |
|
50 |
RAB1A |
5861 |
Proximity Label-MS |
 |
Homo sapiens |
|
51 |
TMEM38B |
|
Proximity Label-MS |
 |
Homo sapiens |
|
52 |
CCDC47 |
57003 |
Proximity Label-MS |
 |
Homo sapiens |
|
53 |
NDUFV1 |
4723 |
Co-fractionation |
 |
Homo sapiens |
|
54 |
TOMM40 |
10452 |
Co-fractionation |
 |
Homo sapiens |
|
55 |
ALCAM |
214 |
Proximity Label-MS |
 |
Homo sapiens |
|
56 |
SLC19A1 |
6573 |
Proximity Label-MS |
 |
Homo sapiens |
|
57 |
SCD5 |
|
Proximity Label-MS |
 |
Homo sapiens |
|
58 |
SLC30A9 |
10463 |
Proximity Label-MS |
 |
Homo sapiens |
|
59 |
FZD7 |
8324 |
Affinity Capture-MS |
 |
Homo sapiens |
|
60 |
Kif2c |
73804 |
Affinity Capture-MS |
 |
Mus musculus |
|
61 |
FAF2 |
23197 |
Co-fractionation |
 |
Homo sapiens |
Proximity Label-MS |
 |
Homo sapiens |
|
62 |
TMEM2 |
23670 |
Proximity Label-MS |
 |
Homo sapiens |
|
63 |
RDH10 |
157506 |
Affinity Capture-MS |
 |
Homo sapiens |
|
64 |
TFRC |
7037 |
Proximity Label-MS |
 |
Homo sapiens |
|
65 |
KIF14 |
9928 |
Affinity Capture-MS |
 |
Homo sapiens |
|
66 |
NOTCH2 |
4853 |
Proximity Label-MS |
 |
Homo sapiens |
|
67 |
TMEM209 |
84928 |
Proximity Label-MS |
 |
Homo sapiens |
|
68 |
RAB1B |
81876 |
Affinity Capture-MS |
 |
Homo sapiens |
|
69 |
LMAN1 |
3998 |
Proximity Label-MS |
 |
Homo sapiens |
|
70 |
PDZD8 |
118987 |
Proximity Label-MS |
 |
Homo sapiens |
|
71 |
E2F4 |
|
Affinity Capture-MS |
 |
Homo sapiens |
|
72 |
LRRC59 |
55379 |
Proximity Label-MS |
 |
Homo sapiens |
|
73 |
LEMD3 |
|
Proximity Label-MS |
 |
Homo sapiens |
|
74 |
VKORC1L1 |
154807 |
Proximity Label-MS |
 |
Homo sapiens |
|
75 |
MRPL32 |
64983 |
Co-fractionation |
 |
Homo sapiens |
|
76 |
CHMP7 |
91782 |
Proximity Label-MS |
 |
Homo sapiens |
|
77 |
HS2ST1 |
9653 |
Affinity Capture-MS |
 |
Homo sapiens |
|
78 |
CANT1 |
124583 |
Proximity Label-MS |
 |
Homo sapiens |
|
79 |
SMPD4 |
55627 |
Proximity Label-MS |
 |
Homo sapiens |
|
80 |
RABL3 |
285282 |
Proximity Label-MS |
 |
Homo sapiens |
|
81 |
EMD |
2010 |
Proximity Label-MS |
 |
Homo sapiens |
|
82 |
SAR1A |
56681 |
Proximity Label-MS |
 |
Homo sapiens |
|
83 |
COPE |
11316 |
Affinity Capture-MS |
 |
Homo sapiens |
|
84 |
HSP90B1 |
7184 |
Proximity Label-MS |
 |
Homo sapiens |
|
85 |
LYN |
4067 |
Proximity Label-MS |
 |
Homo sapiens |
|
86 |
MCAM |
4162 |
Proximity Label-MS |
 |
Homo sapiens |
|
87 |
SNAP47 |
116841 |
Proximity Label-MS |
 |
Homo sapiens |
|
88 |
VAMP3 |
9341 |
Proximity Label-MS |
 |
Homo sapiens |
|
89 |
CERS2 |
29956 |
Proximity Label-MS |
 |
Homo sapiens |
|
90 |
STT3B |
201595 |
Proximity Label-MS |
 |
Homo sapiens |
|
91 |
CDKAL1 |
|
Proximity Label-MS |
 |
Homo sapiens |
|
92 |
VAMP2 |
6844 |
Proximity Label-MS |
 |
Homo sapiens |
|
93 |
APOL2 |
23780 |
Proximity Label-MS |
 |
Homo sapiens |
|
94 |
YIF1A |
10897 |
Proximity Label-MS |
 |
Homo sapiens |
|
95 |
VAPB |
9217 |
Proximity Label-MS |
 |
Homo sapiens |
|
96 |
NTRK1 |
4914 |
Affinity Capture-MS |
 |
Homo sapiens |
|
97 |
GORASP2 |
26003 |
Proximity Label-MS |
 |
Homo sapiens |
|
98 |
SRPR |
6734 |
Proximity Label-MS |
 |
Homo sapiens |
|
99 |
MIA3 |
375056 |
Proximity Label-MS |
 |
Homo sapiens |
|
100 |
P4HB |
5034 |
Proximity Label-MS |
 |
Homo sapiens |
|
101 |
RPN1 |
6184 |
Proximity Label-MS |
 |
Homo sapiens |
|
102 |
ATP6V1B1 |
525 |
Co-fractionation |
 |
Homo sapiens |
|
103 |
GNAI2 |
2771 |
Co-fractionation |
 |
Homo sapiens |
|
104 |
STX5 |
6811 |
Proximity Label-MS |
 |
Homo sapiens |
|
105 |
CTR9 |
9646 |
Affinity Capture-MS |
 |
Homo sapiens |
|
106 |
MRPL11 |
65003 |
Co-fractionation |
 |
Homo sapiens |
|
107 |
CDC42EP1 |
11135 |
Proximity Label-MS |
 |
Homo sapiens |
|
108 |
ATP5B |
506 |
Proximity Label-MS |
 |
Homo sapiens |
|
109 |
VMP1 |
81671 |
Proximity Label-MS |
 |
Homo sapiens |
|
110 |
ACBD3 |
64746 |
Proximity Label-MS |
 |
Homo sapiens |
|
111 |
SLC4A7 |
9497 |
Proximity Label-MS |
 |
Homo sapiens |
|
112 |
REEP5 |
7905 |
Proximity Label-MS |
 |
Homo sapiens |
|
113 |
DUSP22 |
|
Affinity Capture-MS |
 |
Homo sapiens |
|
114 |
LYPD6B |
130576 |
Affinity Capture-MS |
 |
Homo sapiens |
|
115 |
RAB6A |
5870 |
Proximity Label-MS |
 |
Homo sapiens |
|
116 |
ANO6 |
196527 |
Proximity Label-MS |
 |
Homo sapiens |
|
117 |
CYB5B |
80777 |
Proximity Label-MS |
 |
Homo sapiens |
|
118 |
FAM83B |
|
Proximity Label-MS |
 |
Homo sapiens |
|
119 |
ITPR2 |
3709 |
Proximity Label-MS |
 |
Homo sapiens |
|
120 |
ADCY9 |
115 |
Proximity Label-MS |
 |
Homo sapiens |
|
121 |
RPN2 |
6185 |
Proximity Label-MS |
 |
Homo sapiens |
|
122 |
CANX |
821 |
| | | |