Gene ontology annotations for SPCS2
Experiment description of studies that identified SPCS2 in exosomes
1
Experiment ID
489
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule
Protein
Identification method
Mass spectrometry
PubMed ID
36408942
Organism
Rattus norvegicus
Experiment description
Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors
"Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name
Proteomics
Publication year
2023
Sample
Bone marrow mesenchymal stem cells
Sample name
BMSC - Passage 6
Isolation/purification methods
Differential centrifugation Filtration Ultracentrifugation
Flotation density
-
Molecules identified in the study
Protein
Methods used in the study
Western blotting Mass spectometry
2
Experiment ID
490
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule
Protein
Identification method
Mass spectrometry
PubMed ID
36408942
Organism
Rattus norvegicus
Experiment description
Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors
"Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name
Proteomics
Publication year
2023
Sample
Bone marrow mesenchymal stem cells
Sample name
BMSC - Passage 7
Isolation/purification methods
Differential centrifugation Filtration Ultracentrifugation
Flotation density
-
Molecules identified in the study
Protein
Methods used in the study
Western blotting Mass spectometry
3
Experiment ID
491
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule
Protein
Identification method
Mass spectrometry
PubMed ID
36408942
Organism
Rattus norvegicus
Experiment description
Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors
"Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name
Proteomics
Publication year
2023
Sample
Bone marrow mesenchymal stem cells
Sample name
BMSC - Passage 8
Isolation/purification methods
Differential centrifugation Filtration Ultracentrifugation
Flotation density
-
Molecules identified in the study
Protein
Methods used in the study
Western blotting Mass spectometry
4
Experiment ID
492
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule
Protein
Identification method
Mass spectrometry
PubMed ID
36408942
Organism
Rattus norvegicus
Experiment description
Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors
"Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name
Proteomics
Publication year
2023
Sample
Bone marrow mesenchymal stem cells
Sample name
BMSC - Passage 9
Isolation/purification methods
Differential centrifugation Filtration Ultracentrifugation
Flotation density
-
Molecules identified in the study
Protein
Methods used in the study
Western blotting Mass spectometry
5
Experiment ID
426
MISEV standards
✘
Biophysical techniques
✔
SDCBP|FLOT1|CD81|CD9|CD151|FLOT2|TSG101|LAMP1|TFRC|RAB5A|RAB35|GAPDH|UCHL1|ICAM1
Enriched markers
✔
CANX
Negative markers
✘
Particle analysis
Identified molecule
Protein
Identification method
Mass spectrometry
PubMed ID
34108659
Organism
Homo sapiens
Experiment description
Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors
"Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name
Nat Cell Biol
Publication year
2021
Sample
Breast cancer cells
Sample name
MDA-MB-231 - Exo-rich fractions 7-10 pooled
Isolation/purification methods
Differential centrifugation Filtration Ultracentrifugation Size exclusion chromatography
Flotation density
-
Molecules identified in the study
Protein
Methods used in the study
Western blotting Mass spectrometry
6
Experiment ID
427
MISEV standards
✘
Biophysical techniques
✔
CD81|SDCBP|FLOT1|CD9|CD151|FLOT2|TSG101|LAMP1|TFRC|RAB5A|RAB35|GAPDH|UCHL1|ICAM1
Enriched markers
✔
CANX
Negative markers
✘
Particle analysis
Identified molecule
Protein
Identification method
Mass spectrometry
PubMed ID
34108659
Organism
Homo sapiens
Experiment description
Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors
"Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name
Nat Cell Biol
Publication year
2021
Sample
Breast cancer cells
Sample name
MDA-MB-231 - Exo-rich fractions 1-6 pooled
Isolation/purification methods
Differential centrifugation Filtration Ultracentrifugation OptiPrep density gradient centrifugation
Flotation density
-
Molecules identified in the study
Protein
Methods used in the study
Mass spectrometry
7
Experiment ID
419
MISEV standards
✘
Biophysical techniques
✔
SDCBP|FLOT1|CD81|CD9|CD151|FLOT2|TSG101|LAMP1|TFRC|RAB5A|RAB35|GAPDH|UCHL1|ICAM1
Enriched markers
✔
CANX
Negative markers
✘
Particle analysis
Identified molecule
Protein
Identification method
Mass spectrometry
PubMed ID
34108659
Organism
Homo sapiens
Experiment description
Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors
"Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name
Nat Cell Biol
Publication year
2021
Sample
Embryonic kidney cells
Sample name
HEK293T - Exo-rich fractions 7-10 pooled
Isolation/purification methods
Differential centrifugation Filtration Ultracentrifugation Size exclusion chromatography
Flotation density
-
Molecules identified in the study
Protein
Methods used in the study
Western blotting Mass spectrometry
8
Experiment ID
419
MISEV standards
✘
Biophysical techniques
✔
SDCBP|FLOT1|CD81|CD9|CD151|FLOT2|TSG101|LAMP1|TFRC|RAB5A|RAB35|GAPDH|UCHL1|ICAM1
Enriched markers
✔
CANX
Negative markers
✘
Particle analysis
Identified molecule
Protein
Identification method
Mass spectrometry
PubMed ID
34108659
Organism
Homo sapiens
Experiment description
Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors
"Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name
Nat Cell Biol
Publication year
2021
Sample
Embryonic kidney cells
Sample name
HEK293T - Exo-rich fractions 7-10 pooled
Isolation/purification methods
Differential centrifugation Filtration Ultracentrifugation Size exclusion chromatography
Flotation density
-
Molecules identified in the study
Protein
Methods used in the study
Western blotting Mass spectrometry
9
Experiment ID
420
MISEV standards
✘
Biophysical techniques
✔
CD81|SDCBP|FLOT1|CD9|CD151|FLOT2|TSG101|LAMP1|TFRC|RAB5A|RAB35|GAPDH|UCHL1|ICAM1
Enriched markers
✔
CANX
Negative markers
✘
Particle analysis
Identified molecule
Protein
Identification method
Mass spectrometry
PubMed ID
34108659
Organism
Homo sapiens
Experiment description
Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors
"Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name
Nat Cell Biol
Publication year
2021
Sample
Embryonic kidney cells
Sample name
HEK293T - Exo-rich fractions 1-6 pooled
Isolation/purification methods
Differential centrifugation Filtration Ultracentrifugation OptiPrep density gradient centrifugation
Flotation density
-
Molecules identified in the study
Protein
Methods used in the study
Mass spectrometry
10
Experiment ID
420
MISEV standards
✘
Biophysical techniques
✔
CD81|SDCBP|FLOT1|CD9|CD151|FLOT2|TSG101|LAMP1|TFRC|RAB5A|RAB35|GAPDH|UCHL1|ICAM1
Enriched markers
✔
CANX
Negative markers
✘
Particle analysis
Identified molecule
Protein
Identification method
Mass spectrometry
PubMed ID
34108659
Organism
Homo sapiens
Experiment description
Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors
"Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name
Nat Cell Biol
Publication year
2021
Sample
Embryonic kidney cells
Sample name
HEK293T - Exo-rich fractions 1-6 pooled
Isolation/purification methods
Differential centrifugation Filtration Ultracentrifugation OptiPrep density gradient centrifugation
Flotation density
-
Molecules identified in the study
Protein
Methods used in the study
Mass spectrometry
11
Experiment ID
434
MISEV standards
✘
Biophysical techniques
✔
SDCBP|FLOT1|CD81|CD9|CD151|FLOT2|TSG101|LAMP1|TFRC|RAB5A|RAB35|GAPDH|UCHL1|ICAM1
Enriched markers
✔
CANX
Negative markers
✘
Particle analysis
Identified molecule
Protein
Identification method
Mass spectrometry
PubMed ID
34108659
Organism
Homo sapiens
Experiment description
Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors
"Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name
Nat Cell Biol
Publication year
2021
Sample
Pancreatic cancer cells
Sample name
PANC-1 - Exo-rich fractions 7-10 pooled
Isolation/purification methods
Differential centrifugation Filtration Ultracentrifugation Size exclusion chromatography
Flotation density
-
Molecules identified in the study
Protein
Methods used in the study
Western blotting Mass spectrometry
12
Experiment ID
435
MISEV standards
✘
Biophysical techniques
✔
CD81|SDCBP|FLOT1|CD9|CD151|FLOT2|TSG101|LAMP1|TFRC|RAB5A|RAB35|GAPDH|UCHL1|ICAM1
Enriched markers
✔
CANX
Negative markers
✘
Particle analysis
Identified molecule
Protein
Identification method
Mass spectrometry
PubMed ID
34108659
Organism
Homo sapiens
Experiment description
Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors
"Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name
Nat Cell Biol
Publication year
2021
Sample
Pancreatic cancer cells
Sample name
PANC-1 - Exo-rich fractions 1-6 pooled
Isolation/purification methods
Differential centrifugation Filtration Ultracentrifugation OptiPrep density gradient centrifugation
Flotation density
-
Molecules identified in the study
Protein
Methods used in the study
Mass spectrometry
13
Experiment ID
231
MISEV standards
✘
Biophysical techniques
✔
Alix|CD63|CD9
Enriched markers
✘
Negative markers
✔
NTA
Particle analysis
Identified molecule
protein
Identification method
Mass spectrometry
PubMed ID
25332113
Organism
Homo sapiens
Experiment description
Lipidomic and proteomic characterization of platelet extracellular vesicle subfractions from senescent platelets
Authors
"Pienimaeki-Roemer A, Kuhlmann K, Bottcher A, Konovalova T, Black A, Orso E, Liebisch G, Ahrens M, Eisenacher M, Meyer HE, Schmitz G."
Journal name
Transfusion
Publication year
2015
Sample
Platelets
Sample name
PL-Exs - Rep 1
Isolation/purification methods
Differential centrifugation Filtration Ultracentrifugation Optiprep density gradient
Flotation density
1.12-1.15 g/mL
Molecules identified in the study
Protein Lipids
Methods used in the study
Western blotting Mass spectrometry
14
Experiment ID
232
MISEV standards
✘
Biophysical techniques
✘
Enriched markers
✘
Negative markers
✘
Particle analysis
Identified molecule
protein
Identification method
Mass spectrometry
PubMed ID
25332113
Organism
Homo sapiens
Experiment description
Lipidomic and proteomic characterization of platelet extracellular vesicle subfractions from senescent platelets
Authors
"Pienimaeki-Roemer A, Kuhlmann K, Bottcher A, Konovalova T, Black A, Orso E, Liebisch G, Ahrens M, Eisenacher M, Meyer HE, Schmitz G."
Journal name
Transfusion
Publication year
2015
Sample
Platelets
Sample name
PL-Exs - Rep 2
Isolation/purification methods
Differential centrifugation Filtration Ultracentrifugation Optiprep density gradient
Flotation density
1.12-1.15 g/mL
Molecules identified in the study
Protein
Methods used in the study
Mass spectrometry
15
Experiment ID
233
MISEV standards
✘
Biophysical techniques
✘
Enriched markers
✘
Negative markers
✘
Particle analysis
Identified molecule
protein
Identification method
Mass spectrometry
PubMed ID
25332113
Organism
Homo sapiens
Experiment description
Lipidomic and proteomic characterization of platelet extracellular vesicle subfractions from senescent platelets
Authors
"Pienimaeki-Roemer A, Kuhlmann K, Bottcher A, Konovalova T, Black A, Orso E, Liebisch G, Ahrens M, Eisenacher M, Meyer HE, Schmitz G."
Journal name
Transfusion
Publication year
2015
Sample
Platelets
Sample name
PL-Exs - Rep 3
Isolation/purification methods
Differential centrifugation Filtration Ultracentrifugation Optiprep density gradient
Flotation density
1.12-1.15 g/mL
Molecules identified in the study
Protein
Methods used in the study
Mass spectrometry
Protein-protein interactions for SPCS2
Protein Interactor
ExoCarta ID
Identification method
PubMed
Species
1
HNRNPH1
3187
Affinity Capture-RNA
Homo sapiens
2
RIT1
6016
Negative Genetic
Homo sapiens
3
GJD3
Proximity Label-MS
Homo sapiens
4
VAPA
9218
Affinity Capture-MS
Homo sapiens
5
METTL7A
25840
Proximity Label-MS
Homo sapiens
6
LAMP3
Proximity Label-MS
Homo sapiens
7
ATP13A2
Two-hybrid
Homo sapiens
8
RPA2
6118
Proximity Label-MS
Homo sapiens
9
GPC1
2817
Affinity Capture-MS
Homo sapiens
Affinity Capture-MS
Homo sapiens
10
ALPL
249
Affinity Capture-MS
Homo sapiens
Affinity Capture-MS
Homo sapiens
11
HSD17B11
51170
Proximity Label-MS
Homo sapiens
12
PEBP1
5037
Co-fractionation
Homo sapiens
13
TOMM22
56993
Affinity Capture-MS
Homo sapiens
14
AMHR2
Affinity Capture-MS
Homo sapiens
15
PARK2
Affinity Capture-MS
Homo sapiens
16
TM2D3
Affinity Capture-MS
Homo sapiens
Affinity Capture-MS
Homo sapiens
Affinity Capture-MS
Homo sapiens
17
B3GAT1
Proximity Label-MS
Homo sapiens
18
TM9SF4
9777
Co-fractionation
Homo sapiens
19
MCM2
4171
Affinity Capture-MS
Homo sapiens
20
PVRIG
Affinity Capture-MS
Homo sapiens
Affinity Capture-MS
Homo sapiens
Affinity Capture-MS
Homo sapiens
21
SSR1
6745
Proximity Label-MS
Homo sapiens
22
LAMTOR3
8649
Co-fractionation
Homo sapiens
23
RPN2
6185
Proximity Label-MS
Homo sapiens
24
CCDC47
57003
Affinity Capture-MS
Homo sapiens
25
SEC11C
90701
Affinity Capture-MS
Homo sapiens
Affinity Capture-MS
Homo sapiens
Affinity Capture-MS
Homo sapiens
Affinity Capture-MS
Homo sapiens
26
PRTN3
5657
Affinity Capture-MS
Homo sapiens
27
CLN8
2055
Two-hybrid
Homo sapiens
28
LMAN1L
Affinity Capture-MS
Homo sapiens
29
TMEM87A
25963
Co-fractionation
Homo sapiens
30
KIAA1715
80856
Proximity Label-MS
Homo sapiens
31
AURKA
6790
Affinity Capture-MS
Homo sapiens
32
PLAUR
5329
Affinity Capture-MS
Homo sapiens
33
HIST1H4A
8359
Affinity Capture-MS
Homo sapiens
34
WNT6
Affinity Capture-MS
Homo sapiens
35
AMFR
267
Affinity Capture-MS
Homo sapiens
36
BTN2A3P
Affinity Capture-MS
Homo sapiens
37
C12orf49
Positive Genetic
Homo sapiens
38
GPC5
2262
Affinity Capture-MS
Homo sapiens
39
TCTN2
Affinity Capture-MS
Homo sapiens
Affinity Capture-MS
Homo sapiens
40
MIA3
375056
Affinity Capture-MS
Homo sapiens
Affinity Capture-MS
Homo sapiens
41
RPN1
6184
Proximity Label-MS
Homo sapiens
42
RNF13
11342
Affinity Capture-MS
Homo sapiens
43
ELOVL5
60481
Proximity Label-MS
Homo sapiens
44
SEC61B
10952
Proximity Label-MS
Homo sapiens
45
CD274
29126
Affinity Capture-MS
Homo sapiens
46
TMEM14C
51522
Co-fractionation
Homo sapiens
47
LYPD6B
130576
Affinity Capture-MS
Homo sapiens
48
MRPL44
Co-fractionation
Homo sapiens
49
HSPA5
3309
Affinity Capture-MS
Homo sapiens
50
EFNA4
Affinity Capture-MS
Homo sapiens
51
GPC4
2239
Affinity Capture-MS
Homo sapiens
Affinity Capture-MS
Homo sapiens
52
HADHB
3032
Co-fractionation
Homo sapiens
53
TTYH1
Affinity Capture-MS
Homo sapiens
54
CANX
821
Affinity Capture-MS
Homo sapiens
55
LRRC59
55379
Proximity Label-MS
Homo sapiens
56
LMNB1
4001
Proximity Label-MS
Homo sapiens
57
RTN4R
65078
Affinity Capture-MS
Homo sapiens
58
GOLT1B
51026
Affinity Capture-MS
Homo sapiens
59
BCAP31
10134
Proximity Label-MS
Homo sapiens
60
PRSS50
Affinity Capture-MS
Homo sapiens
Affinity Capture-MS
Homo sapiens
61
EGFR
1956
PCA
Homo sapiens
62
FBXO6
26270
Affinity Capture-MS
Homo sapiens
63
SIRPG
55423
Affinity Capture-MS
Homo sapiens
64
TOMM6
Co-fractionation
Homo sapiens
65
TREM1
54210
Affinity Capture-MS
Homo sapiens
66
RPA3
6119
Proximity Label-MS
Homo sapiens
67
ATP2A1
487
Proximity Label-MS
Homo sapiens
68
SYVN1
84447
Affinity Capture-MS
Homo sapiens
69
NCLN
56926
Affinity Capture-MS
Homo sapiens
70
DHFRL1
Proximity Label-MS
Homo sapiens
71
DNASE1L1
1774
Affinity Capture-MS
Homo sapiens
Affinity Capture-MS
Homo sapiens
Affinity Capture-MS
Homo sapiens
72
NRN1
Affinity Capture-MS
Homo sapiens
73
STK4
6789
Affinity Capture-MS
Homo sapiens
74
HUWE1
10075
Affinity Capture-MS
Homo sapiens
75
ADAM30
11085
Affinity Capture-MS
Homo sapiens
Affinity Capture-MS
Homo sapiens
76
SPCS3
60559
Affinity Capture-MS
Homo sapiens
Affinity Capture-MS
Homo sapiens
77
TSPAN5
10098
Affinity Capture-MS
Homo sapiens
Affinity Capture-MS
Homo sapiens
78
VNN2
Affinity Capture-MS
Homo sapiens
Affinity Capture-MS
Homo sapiens
79
KCNA2
Affinity Capture-MS
Homo sapiens
80
ILF3
3609
Co-fractionation
Homo sapiens
81
GABRE
Affinity Capture-MS
Homo sapiens
Affinity Capture-MS
Homo sapiens
82
MCAT
27349
Co-fractionation
Homo sapiens
83
TP53
7157
Affinity Capture-MS
Homo sapiens
84
PGRMC1
10857
Co-fractionation
Homo sapiens
Affinity Capture-MS
Homo sapiens
85
SEC11A
23478
Affinity Capture-MS
Homo sapiens
Affinity Capture-MS
Homo sapiens
86
FBXL14
Affinity Capture-MS
Homo sapiens
Affinity Capture-MS
Homo sapiens
87
HSD3B7
80270
Proximity Label-MS
Homo sapiens
88
MGAT4C
Affinity Capture-MS
Homo sapiens
Affinity Capture-MS
Homo sapiens
89
NUP35
129401
Proximity Label-MS
Homo sapiens
90
ERGIC1
57222
Proximity Label-MS
Homo sapiens
91
SPCS1
28972
Affinity Capture-MS
Homo sapiens
Affinity Capture-MS
Homo sapiens
92
SEC62
7095
Proximity Label-MS
Homo sapiens
93
HNRNPH3
3189
Affinity Capture-MS
Homo sapiens
94
C16orf72
29035
Affinity Capture-MS
Homo sapiens
95
RAB5C
5878
Proximity Label-MS
Homo sapiens
96
C9orf72
Affinity Capture-MS
Homo sapiens
97
CKAP4
10970
Proximity Label-MS
Homo sapiens
98
ARSK
Affinity Capture-MS
Homo sapiens
99
DERL1
79139
Proximity Label-MS
Homo sapiens
View the network
image/svg+xml
Pathways in which SPCS2 is involved