Gene description for SPCS3
Gene name signal peptidase complex subunit 3 homolog (S. cerevisiae)
Gene symbol SPCS3
Other names/aliases PRO3567
SPC22/23
SPC3
YLR066W
Species Homo sapiens
 Database cross references - SPCS3
ExoCarta ExoCarta_60559
Vesiclepedia VP_60559
Entrez Gene 60559
HGNC 26212
UniProt P61009  
 SPCS3 identified in exosomes derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Endothelial cells 26027894    
Platelets 25332113    
Platelets 25332113    
Platelets 25332113    
Retinal pigment epithelial cells 35333565    
Retinal pigment epithelial cells 35333565    
 Gene ontology annotations for SPCS3
Molecular Function
    protein binding GO:0005515 IPI
Biological Process
    signal peptide processing GO:0006465 IBA
    signal peptide processing GO:0006465 IDA
    proteolysis GO:0006508 IDA
    viral protein processing GO:0019082 IMP
    protein targeting to ER GO:0045047 IBA
Subcellular Localization
    endoplasmic reticulum GO:0005783 IDA
    signal peptidase complex GO:0005787 IBA
    signal peptidase complex GO:0005787 IPI
    endoplasmic reticulum membrane GO:0005789 IDA
    endoplasmic reticulum membrane GO:0005789 TAS
 Experiment description of studies that identified SPCS3 in exosomes
1
Experiment ID 489
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
5
Experiment ID 226
MISEV standards
✔
EM
Biophysical techniques
✔
GAPDH|CD9|FLOT1
Enriched markers
✔
LMNA|H2AFX|ATP5A1|TOMM20
Negative markers
✘ Particle analysis
Identified molecule mRNA
Identification method Small RNA sequencing (Illumina HiSeq 2000 (Solexa)
PubMed ID 26027894    
Organism Homo sapiens
Experiment description "Quantitative and qualitative analysis of small RNAs in human endothelial cells and exosomes provides insights into localized RNA processing, degradation and sorting"
Authors "Bas W. M. van Balkom, Almut S. Eisele, D. Michiel Pegtel, Sander Bervoets, Marianne C. Verhaar"
Journal name Journal of Extracellular Vesicles
Publication year 2015
Sample Endothelial cells
Sample name HMEC-1
Isolation/purification methods Differential ultracentrifugation
Sucrose density gradient
Flotation density 1.10 g/mL
Molecules identified in the study miRNA
Protein
snoRNA
lncRNA
yRNA
snRNA
mRNA
ncRNA
mtRNA
vtRNA
scaRNA
lincRNA
Methods used in the study Small RNA sequencing (Illumina HiSeq 2000 (Solexa)
Western blotting
6
Experiment ID 231
MISEV standards
✘ Biophysical techniques
✔
Alix|CD63|CD9
Enriched markers
✘ Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25332113    
Organism Homo sapiens
Experiment description Lipidomic and proteomic characterization of platelet extracellular vesicle subfractions from senescent platelets
Authors "Pienimaeki-Roemer A, Kuhlmann K, Bottcher A, Konovalova T, Black A, Orso E, Liebisch G, Ahrens M, Eisenacher M, Meyer HE, Schmitz G."
Journal name Transfusion
Publication year 2015
Sample Platelets
Sample name PL-Exs - Rep 1
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Optiprep density gradient
Flotation density 1.12-1.15 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Western blotting
Mass spectrometry
7
Experiment ID 232
MISEV standards
✘ Biophysical techniques
✘ Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25332113    
Organism Homo sapiens
Experiment description Lipidomic and proteomic characterization of platelet extracellular vesicle subfractions from senescent platelets
Authors "Pienimaeki-Roemer A, Kuhlmann K, Bottcher A, Konovalova T, Black A, Orso E, Liebisch G, Ahrens M, Eisenacher M, Meyer HE, Schmitz G."
Journal name Transfusion
Publication year 2015
Sample Platelets
Sample name PL-Exs - Rep 2
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Optiprep density gradient
Flotation density 1.12-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
8
Experiment ID 233
MISEV standards
✘ Biophysical techniques
✘ Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25332113    
Organism Homo sapiens
Experiment description Lipidomic and proteomic characterization of platelet extracellular vesicle subfractions from senescent platelets
Authors "Pienimaeki-Roemer A, Kuhlmann K, Bottcher A, Konovalova T, Black A, Orso E, Liebisch G, Ahrens M, Eisenacher M, Meyer HE, Schmitz G."
Journal name Transfusion
Publication year 2015
Sample Platelets
Sample name PL-Exs - Rep 3
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Optiprep density gradient
Flotation density 1.12-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
9
Experiment ID 834
MISEV standards
✔
EM
Biophysical techniques
✔
LAMP2|CD63|FLOT1|ITGA2B|ICAM1|CD9|CD151|TFRC|RAB5A|GAPDH|AQP1|TSG101
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35333565    
Organism Homo sapiens
Experiment description LAMP2A regulates the loading of proteins into exosomes
Authors "Ferreira JV, da Rosa Soares A, Ramalho J, Máximo Carvalho C, Cardoso MH, Pintado P, Carvalho AS, Beck HC, Matthiesen R, Zuzarte M, Girão H, van Niel G, Pereira P"
Journal name Sci Adv
Publication year 2022
Sample Retinal pigment epithelial cells
Sample name ARPE-19
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
10
Experiment ID 835
MISEV standards
✔
EM
Biophysical techniques
✔
CD63|FLOT1|ITGA2B|ICAM1|CD9|CD151|TFRC|RAB5A|GAPDH|AQP1|TSG101
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35333565    
Organism Homo sapiens
Experiment description LAMP2A regulates the loading of proteins into exosomes
Authors "Ferreira JV, da Rosa Soares A, Ramalho J, Máximo Carvalho C, Cardoso MH, Pintado P, Carvalho AS, Beck HC, Matthiesen R, Zuzarte M, Girão H, van Niel G, Pereira P"
Journal name Sci Adv
Publication year 2022
Sample Retinal pigment epithelial cells
Sample name ARPE-19
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for SPCS3
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 SEC11C 90701
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
2 UBR2 23304
Affinity Capture-MS Homo sapiens
3 XRCC3  
Affinity Capture-MS Homo sapiens
4 RAP2B 5912
Affinity Capture-MS Homo sapiens
5 HSPA5 3309
Affinity Capture-MS Homo sapiens
6 SEC63 11231
Proximity Label-MS Homo sapiens
7 RAB6B 51560
Affinity Capture-MS Homo sapiens
8 TNIK 23043
Affinity Capture-MS Homo sapiens
9 ABCB6 10058
Affinity Capture-MS Homo sapiens
10 NUBPL 80224
Affinity Capture-MS Homo sapiens
11 LAMA3 3909
Affinity Capture-MS Homo sapiens
12 VAPB 9217
Affinity Capture-MS Homo sapiens
13 MADD 8567
Affinity Capture-MS Homo sapiens
14 CANX 821
Affinity Capture-MS Homo sapiens
15 VAPA 9218
Affinity Capture-MS Homo sapiens
16 PGRMC1 10857
Affinity Capture-MS Homo sapiens
17 LRRC59 55379
Proximity Label-MS Homo sapiens
18 ENTPD6  
Affinity Capture-MS Homo sapiens
19 PDIA4 9601
Proximity Label-MS Homo sapiens
20 NOMO1 23420
Affinity Capture-MS Homo sapiens
21 DSTYK 25778
Affinity Capture-MS Homo sapiens
22 SEC11A 23478
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
23 MAN1B1 11253
Affinity Capture-MS Homo sapiens
24 GPC1 2817
Affinity Capture-MS Homo sapiens
25 CST8  
Affinity Capture-MS Homo sapiens
26 Spcs2 66624
Affinity Capture-MS Mus musculus
27 DNLZ  
Affinity Capture-MS Homo sapiens
28 LRP11  
Affinity Capture-MS Homo sapiens
29 NR3C1 2908
Proximity Label-MS Homo sapiens
30 MECP2 4204
Affinity Capture-MS Homo sapiens
31 FKBP8 23770
Proximity Label-MS Homo sapiens
32 MEGF8 1954
Affinity Capture-MS Homo sapiens
33 PARK2  
Affinity Capture-MS Homo sapiens
34 FBXO6 26270
Affinity Capture-MS Homo sapiens
35 DNAJC25 548645
Proximity Label-MS Homo sapiens
36 TUBB3 10381
Affinity Capture-MS Homo sapiens
37 LRRC41 10489
Affinity Capture-MS Homo sapiens
38 HTRA3 94031
Affinity Capture-MS Homo sapiens
39 MIA3 375056
Affinity Capture-MS Homo sapiens
40 HLA-DPB1 3115
Affinity Capture-MS Homo sapiens
41 SLC22A14  
Cross-Linking-MS (XL-MS) Homo sapiens
42 NEU1 4758
Affinity Capture-MS Homo sapiens
43 RAP2C 57826
Affinity Capture-MS Homo sapiens
44 TUBA1A 7846
Affinity Capture-MS Homo sapiens
45 PGRMC2 10424
Affinity Capture-MS Homo sapiens
46 TMEM132A 54972
Affinity Capture-MS Homo sapiens
47 CSPG4 1464
Affinity Capture-MS Homo sapiens
48 UBR1 197131
Affinity Capture-MS Homo sapiens
49 SPCS1 28972
Affinity Capture-MS Homo sapiens
50 RPA2 6118
Proximity Label-MS Homo sapiens
51 PC 5091
Affinity Capture-MS Homo sapiens
52 RAN 5901
Affinity Capture-MS Homo sapiens
53 PTPN1 5770
Proximity Label-MS Homo sapiens
54 MCM2 4171
Affinity Capture-MS Homo sapiens
55 PRKD2 25865
Affinity Capture-MS Homo sapiens
56 CYB5R3 1727
Affinity Capture-MS Homo sapiens
57 NCLN 56926
Affinity Capture-MS Homo sapiens
58 SF3B6 51639
Cross-Linking-MS (XL-MS) Homo sapiens
59 CTAGE5 4253
Affinity Capture-MS Homo sapiens
60 DNAJC1 64215
Proximity Label-MS Homo sapiens
61 KRAS 3845
Negative Genetic Homo sapiens
62 C9orf72  
Affinity Capture-MS Homo sapiens
63 OS9 10956
Affinity Capture-MS Homo sapiens
64 TMEM147 10430
Affinity Capture-MS Homo sapiens
65 STK4 6789
Affinity Capture-MS Homo sapiens
66 VDAC3 7419
Co-fractionation Homo sapiens
67 C16orf58 64755
Affinity Capture-MS Homo sapiens
68 ANKRD46 157567
Affinity Capture-MS Homo sapiens
69 RTN4R 65078
Affinity Capture-MS Homo sapiens
70 RPA3 6119
Proximity Label-MS Homo sapiens
71 RHOT2 89941
Proximity Label-MS Homo sapiens
72 PTPRO 5800
Affinity Capture-MS Homo sapiens
73 DDOST 1650
Affinity Capture-MS Homo sapiens
74 SEC61B 10952
Proximity Label-MS Homo sapiens
75 SPCS2 9789
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
76 SFXN2  
Affinity Capture-MS Homo sapiens
77 TCP11L1 55346
Affinity Capture-MS Homo sapiens
78 GGT7 2686
Affinity Capture-MS Homo sapiens
79 GNB2 2783
Affinity Capture-MS Homo sapiens
View the network image/svg+xml



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