Gene description for HTRA3
Gene name HtrA serine peptidase 3
Gene symbol HTRA3
Other names/aliases Prsp
Tasp
Species Homo sapiens
 Database cross references - HTRA3
ExoCarta ExoCarta_94031
Entrez Gene 94031
HGNC 30406
MIM 608785
UniProt P83110  
 HTRA3 identified in exosomes derived from the following tissue/cell type
Hepatocellular carcinoma cells 26054723    
 Gene ontology annotations for HTRA3
Molecular Function
    serine-type peptidase activity GO:0008236 IDA
    protein binding GO:0005515 IPI
    endopeptidase activity GO:0004175 IDA
    insulin-like growth factor binding GO:0005520 IEA
    serine-type endopeptidase activity GO:0004252 IEA
Biological Process
    negative regulation of transforming growth factor beta receptor signaling pathway GO:0030512 ISS
    regulation of cell growth GO:0001558 IEA
    negative regulation of BMP signaling pathway GO:0030514 ISS
    proteolysis GO:0006508 IDA
Subcellular Localization
    extracellular region GO:0005576 IEA
 Experiment description of studies that identified HTRA3 in exosomes
1
Experiment ID 234
ISEV standards
EM
EV Biophysical techniques
TSG101|Alix|HSC70|GAPDH
EV Cytosolic markers
EV Membrane markers
HSP90B1
EV Negative markers
qNano
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 26054723    
Organism Homo sapiens
Experiment description Hepatocellular carcinoma-derived exosomes promote motility of immortalized hepatocyte through transfer of oncogenic proteins and RNAs
Authors He M, Qin H, Poon TC, Sze SC, Ding X, Co NN, Ngai SM, Chan TF, Wong N
Journal name Carcinogenesis
Publication year 2015
Sample Hepatocellular carcinoma cells
Sample name HKCI-C3
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
RNA
Methods used in the study Western blotting
Mass spectrometry
RT-PCR
RNA Sequencing
 Protein-protein interactions for HTRA3
  Protein Interactor ExoCarta ID Identification method PubMed Species
No interactions are found.
 Pathways in which HTRA3 is involved
No pathways found





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