Gene description for TMEM147
Gene name transmembrane protein 147
Gene symbol TMEM147
Other names/aliases NIFIE14
Species Homo sapiens
 Database cross references - TMEM147
ExoCarta ExoCarta_10430
Vesiclepedia VP_10430
Entrez Gene 10430
HGNC 30414
MIM 613585
UniProt Q9BVK8  
 TMEM147 identified in exosomes derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
 Gene ontology annotations for TMEM147
Molecular Function
    protein binding GO:0005515 IPI
    ribosome binding GO:0043022 IDA
Biological Process
    protein localization to nuclear inner membrane GO:0036228 IDA
    multi-pass transmembrane protein insertion into ER membrane GO:0160063 IDA
Subcellular Localization
    endoplasmic reticulum membrane GO:0005789 IDA
    endoplasmic reticulum membrane GO:0005789 IDA
    plasma membrane GO:0005886 ISS
    nuclear membrane GO:0031965 IDA
    protein-containing complex GO:0032991 IDA
    multi-pass translocon complex GO:0160064 IDA
 Experiment description of studies that identified TMEM147 in exosomes
1
Experiment ID 489
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
 Protein-protein interactions for TMEM147
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 TMEM52B 120939
Two-hybrid Homo sapiens
2 F11R 50848
Two-hybrid Homo sapiens
3 RPL22 6146
Affinity Capture-MS Homo sapiens
4 ABHD16A 7920
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
5 GJA8  
Two-hybrid Homo sapiens
6 ABI3 51225
Two-hybrid Homo sapiens
7 FASN 2194
Negative Genetic Homo sapiens
8 CLN8 2055
Two-hybrid Homo sapiens
9 TACR1  
Two-hybrid Homo sapiens
10 PDCD1LG2 80380
Two-hybrid Homo sapiens
11 NOMO3 408050
Affinity Capture-MS Homo sapiens
12 FFAR2  
Two-hybrid Homo sapiens
13 LHFPL2 10184
Two-hybrid Homo sapiens
14 RTKN 6242
Affinity Capture-MS Homo sapiens
15 LDLRAD1  
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
16 PLEKHG3 26030
Affinity Capture-MS Homo sapiens
17 MTIF3  
Two-hybrid Homo sapiens
18 PSCA 8000
Two-hybrid Homo sapiens
19 TMEM248 55069
Two-hybrid Homo sapiens
20 CLRN1  
Two-hybrid Homo sapiens
21 SHISA3  
Two-hybrid Homo sapiens
22 GLP1R  
PCA Homo sapiens
Affinity Capture-Western Homo sapiens
23 CLDN7 1366
Two-hybrid Homo sapiens
24 TMEM14B  
Two-hybrid Homo sapiens
25 SYNE4  
Two-hybrid Homo sapiens
26 GPR101  
Two-hybrid Homo sapiens
27 EFNA4  
Two-hybrid Homo sapiens
28 CD40 958
Two-hybrid Homo sapiens
29 TMEM42  
Two-hybrid Homo sapiens
30 EBP  
Two-hybrid Homo sapiens
31 CD79A  
Two-hybrid Homo sapiens
32 RPL32 6161
Affinity Capture-MS Homo sapiens
33 IL3RA  
Two-hybrid Homo sapiens
34 CREB3L1  
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
35 SUSD3 203328
Two-hybrid Homo sapiens
36 DPM3 54344
Two-hybrid Homo sapiens
37 LEUTX  
Two-hybrid Homo sapiens
38 PPP1R9A  
Affinity Capture-MS Homo sapiens
39 LRRFIP2 9209
Affinity Capture-MS Homo sapiens
40 CCDC155  
Two-hybrid Homo sapiens
41 GPR42  
Two-hybrid Homo sapiens
42 RPL38 6169
Affinity Capture-MS Homo sapiens
43 HTR2C  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Two-hybrid Homo sapiens
44 TMEM237 65062
Two-hybrid Homo sapiens
45 RPS23 6228
Affinity Capture-MS Homo sapiens
46 PRKCDBP 112464
Affinity Capture-MS Homo sapiens
47 TMCO1 54499
Affinity Capture-Western Homo sapiens
48 SFT2D3  
Affinity Capture-MS Homo sapiens
49 CAV2 858
Affinity Capture-MS Homo sapiens
50 SLC13A4  
Two-hybrid Homo sapiens
51 SLC10A1  
Two-hybrid Homo sapiens
52 RPS8 6202
Affinity Capture-MS Homo sapiens
53 NCLN 56926
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
54 SLC12A7 10723
Two-hybrid Homo sapiens
55 GPR61  
Two-hybrid Homo sapiens
56 CGN  
Affinity Capture-MS Homo sapiens
57 MUC1 4582
Two-hybrid Homo sapiens
58 KTN1 3895
Affinity Capture-MS Homo sapiens
59 GOLM1 51280
Two-hybrid Homo sapiens
60 TMEM252  
Two-hybrid Homo sapiens
61 MPRIP 23164
Affinity Capture-MS Homo sapiens
62 RNF185  
Two-hybrid Homo sapiens
63 GPR37L1  
Two-hybrid Homo sapiens
64 TEX29  
Two-hybrid Homo sapiens
65 TSPAN12  
Two-hybrid Homo sapiens
66 TMEM206 55248
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
67 CXCR2  
Two-hybrid Homo sapiens
68 MARS 4141
Affinity Capture-MS Homo sapiens
69 SPCS3 60559
Affinity Capture-MS Homo sapiens
70 RMDN3 55177
Two-hybrid Homo sapiens
71 CCDC47 57003
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
72 SCAMP4 113178
Two-hybrid Homo sapiens
View the network image/svg+xml
 Pathways in which TMEM147 is involved
No pathways found





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