Gene description for CLDN7
Gene name claudin 7
Gene symbol CLDN7
Other names/aliases CEPTRL2
CLDN-7
CPETRL2
Hs.84359
claudin-1
Species Homo sapiens
 Database cross references - CLDN7
ExoCarta ExoCarta_1366
Vesiclepedia VP_1366
Entrez Gene 1366
HGNC 2049
MIM 609131
UniProt O95471  
 CLDN7 identified in sEVs derived from the following tissue/cell type
Colorectal cancer cells 19837982    
Colorectal cancer cells 34887515    
Hepatocytes 26054723    
 Gene ontology annotations for CLDN7
Molecular Function
    structural molecule activity GO:0005198 IEA
    protein binding GO:0005515 IPI
    protein domain specific binding GO:0019904 IEA
    identical protein binding GO:0042802 ISS
    cell adhesion molecule binding GO:0050839 IEA
Biological Process
    cell adhesion GO:0007155 IBA
    negative regulation of cell adhesion GO:0007162 IEA
    positive regulation of cell population proliferation GO:0008284 IEA
    calcium-independent cell-cell adhesion via plasma membrane cell-adhesion molecules GO:0016338 ISS
    negative regulation of protein-containing complex assembly GO:0031333 IEA
    negative regulation of apoptotic process GO:0043066 IEA
    response to ethanol GO:0045471 IEA
    bicellular tight junction assembly GO:0070830 IBA
    positive regulation of cell motility GO:2000147 IEA
Subcellular Localization
    plasma membrane GO:0005886 IBA
    plasma membrane GO:0005886 IDA
    bicellular tight junction GO:0005923 IBA
    bicellular tight junction GO:0005923 ISS
    basolateral plasma membrane GO:0016323 IEA
    apicolateral plasma membrane GO:0016327 IEA
    lateral plasma membrane GO:0016328 IEA
 Experiment description of studies that identified CLDN7 in sEVs
1
Experiment ID 21
MISEV standards
✔
EM|IEM
Biophysical techniques
✔
Alix|TSG101|HSP70|CD63
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method
Mass spectrometry   
PubMed ID 19837982    
Organism Homo sapiens
Experiment description Proteomic and bioinformatic analysis of immunoaffinity-purified exosomes derived from the human colon tumor cell line LIM1215.
Authors "Suresh Mathivanan, Justin W.E. Lim, Bow J. Tauro, Hong Ji, Robert L. Moritz and Richard J. Simpson"
Journal name MCP
Publication year 2009
Sample Colorectal cancer cells
Sample name LIM1215
Isolation/purification methods Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.10-1.12 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [Orbitrap]
Western blotting
2
Experiment ID 1203
MISEV standards
✔
EM
Biophysical techniques
✔
SDCBP|FLOT1|CD9|CD81|CD63|EPCAM|GAPDH|LAMP1|TFRC|CD151|CD82|LAMP2|RAB35|TSG101|FLOT2|RAB5B|ICAM1|RAB5A
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 34887515    
Organism Homo sapiens
Experiment description Supermeres are functional extracellular nanoparticles replete with disease biomarkers and therapeutic targets
Authors "Zhang Q, Jeppesen DK, Higginbotham JN, Graves-Deal R, Trinh VQ, Ramirez MA, Sohn Y, Neininger AC, Taneja N, McKinley ET, Niitsu H, Cao Z, Evans R, Glass SE, Ray KC, Fissell WH, Hill S, Rose KL, Huh WJ, Washington MK, Ayers GD, Burnette DT, Sharma S, Rome LH, Franklin JL, Lee YA, Liu Q, Coffey RJ."
Journal name Nat Cell Biol
Publication year 2021
Sample Colorectal cancer cells
Sample name DiFi
Isolation/purification methods Differential centrifugation
Filtration
Centrifugal ultrafiltration
Ultracentrifugation
OptiPrep density gradient centrifugation
Flotation density -
Molecules identified in the study Protein
miRNA
Methods used in the study Western blotting
Mass spectrometry
RNA sequencing
3
Experiment ID 237
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|Alix|HSC70|GAPDH
Enriched markers
✔
HSP90B1
Negative markers
✔
qNano
Particle analysis
Identified molecule mRNA
Identification method RNA Sequencing
PubMed ID 26054723    
Organism Homo sapiens
Experiment description Hepatocellular carcinoma-derived exosomes promote motility of immortalized hepatocyte through transfer of oncogenic proteins and RNAs
Authors "He M, Qin H, Poon TC, Sze SC, Ding X, Co NN, Ngai SM, Chan TF, Wong N"
Journal name Carcinogenesis
Publication year 2015
Sample Hepatocytes
Sample name MIHA
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
RNA
Methods used in the study Western blotting
Mass spectrometry
RNA Sequencing
 Protein-protein interactions for CLDN7
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 SEC22B 9554
Two-hybrid Homo sapiens
2 UPK2 7379
Two-hybrid Homo sapiens
3 TMEM60  
Two-hybrid Homo sapiens
4 BET1 10282
Two-hybrid Homo sapiens
5 TJP1 7082
Reconstituted Complex Homo sapiens
6 TMEM44  
Two-hybrid Homo sapiens
7 LAT 27040
Two-hybrid Homo sapiens
8 COL4A5  
Two-hybrid Homo sapiens
9 MAL  
Two-hybrid Homo sapiens
10 CYB5B 80777
Two-hybrid Homo sapiens
11 UPK1B 7348
Two-hybrid Homo sapiens
12 BMP10  
Two-hybrid Homo sapiens
13 JAGN1 84522
Two-hybrid Homo sapiens
14 TACSTD2 4070
Affinity Capture-MS Homo sapiens
15 LEPROTL1 23484
Two-hybrid Homo sapiens
16 C14orf1 11161
Two-hybrid Homo sapiens
17 GIMAP5  
Two-hybrid Homo sapiens
18 MFSD5  
Two-hybrid Homo sapiens
19 TNF  
Two-hybrid Homo sapiens
20 RTP2  
Two-hybrid Homo sapiens
21 EPCAM 4072
Affinity Capture-MS Homo sapiens
22 CD72  
Two-hybrid Homo sapiens
23 CD53 963
Two-hybrid Homo sapiens
24 TUSC5  
Two-hybrid Homo sapiens
25 CYB561A3 220002
Two-hybrid Homo sapiens
26 HGD 3081
Two-hybrid Homo sapiens
27 TMEM147 10430
Two-hybrid Homo sapiens
28 ASGR1 432
Two-hybrid Homo sapiens
29 GLP1R  
PCA Homo sapiens
30 TMEM128  
Two-hybrid Homo sapiens
31 SYNE4  
Two-hybrid Homo sapiens
32 TSPAN15 23555
Affinity Capture-MS Homo sapiens
33 CYB561D2 11068
Two-hybrid Homo sapiens
34 AIG1  
Two-hybrid Homo sapiens
35 TSPAN33 340348
Two-hybrid Homo sapiens
36 VSTM1  
Two-hybrid Homo sapiens
37 UNC93B1 81622
Two-hybrid Homo sapiens
38 IGFBP5 3488
Two-hybrid Homo sapiens
39 FAXDC2  
Two-hybrid Homo sapiens
40 NIPAL4  
Two-hybrid Homo sapiens
41 FXYD6  
Two-hybrid Homo sapiens
42 TMEM11 8834
Two-hybrid Homo sapiens
43 OLFM4 10562
Two-hybrid Homo sapiens
44 SLC38A7 55238
Two-hybrid Homo sapiens
45 APOD 347
Two-hybrid Homo sapiens
46 TMEM100 55273
Two-hybrid Homo sapiens
47 MS4A13  
Two-hybrid Homo sapiens
48 NNT 23530
Affinity Capture-MS Homo sapiens
49 LPAR3  
Two-hybrid Homo sapiens
50 CXCL16 58191
Two-hybrid Homo sapiens
51 PLN  
Two-hybrid Homo sapiens
52 LSMEM2  
Two-hybrid Homo sapiens
53 RHOXF2  
Two-hybrid Homo sapiens
54 AQP2 359
Two-hybrid Homo sapiens
55 RPRM  
Two-hybrid Homo sapiens
56 BCL2L2 599
Two-hybrid Homo sapiens
57 CMTM5  
Two-hybrid Homo sapiens
58 GPR37L1  
Two-hybrid Homo sapiens
59 MALL  
Two-hybrid Homo sapiens
60 EDDM3B  
Two-hybrid Homo sapiens
61 VAMP5 10791
Two-hybrid Homo sapiens
62 PGAP2  
Two-hybrid Homo sapiens
63 CXCL9  
Two-hybrid Homo sapiens
64 EMP3 2014
Two-hybrid Homo sapiens
65 SERTM1  
Two-hybrid Homo sapiens
66 NKG7  
Two-hybrid Homo sapiens
67 ANKRD46 157567
Two-hybrid Homo sapiens
68 C17orf62 79415
Two-hybrid Homo sapiens
69 UBIAD1 29914
Two-hybrid Homo sapiens
70 NDUFB11 54539
Two-hybrid Homo sapiens
71 C3orf52  
Two-hybrid Homo sapiens
72 SCARB1 949
Affinity Capture-MS Homo sapiens
73 TMEM97 27346
Two-hybrid Homo sapiens
74 SCAMP4 113178
Two-hybrid Homo sapiens
75 TMEM140  
Two-hybrid Homo sapiens
76 CLN6  
Two-hybrid Homo sapiens
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