Experiment description of studies that identified CD53 in exosomes

1 Experiment ID 16 MISEV standards ✔ EM EV Biophysical techniques ✔ MHCII|CD86|LAMP1|LAMP2|CD63|CD81|CD82|ICAM1|TFRC EV Enriched markers ✘ EV Negative markers ✘ EV Particle analysis Identified molecule protein Identification method Immunoelectron Microscopy PubMed ID 9685355     Organism Homo sapiens Experiment description Selective enrichment of tetraspan proteins on the internal vesicles of multivesicular endosomes and on exosomes secreted by human B-lymphocytes. Authors Escola JM, Kleijmeer MJ, Stoorvogel W, Griffith JM, Yoshie O, Geuze HJ Journal name JBC Publication year 1998 Sample B cells Sample name RN (HLA-DR15+) Isolation/purification methods Differential centrifugation Sucrose density gradient Flotation density 1.12-1.18 g/mL Molecules identified in the study Protein Methods used in the study Western blotting Immunoelectron Microscopy

2 Experiment ID 79 MISEV standards ✘ EV Biophysical techniques ✔ CD81|MHCII EV Enriched markers ✘ EV Negative markers ✘ EV Particle analysis Identified molecule protein Identification method Mass spectrometry PubMed ID 20458337     Organism Homo sapiens Experiment description MHC class II-associated proteins in B-cell exosomes and potential functional implications for exosome biogenesis - Sample 1 Authors Buschow SI, van Balkom BW, Aalberts M, Heck AJ, Wauben M, Stoorvogel W. Journal name ICB Publication year 2010 Sample B cells Sample name RN (HLA-DR15) Isolation/purification methods Differential centrifugation Sucrose density gradient Immunobeads (MHC Class II) Flotation density - Molecules identified in the study Protein Methods used in the study Mass spectrometry [FT-ICR] Western blotting

3 Experiment ID 81 MISEV standards ✘ EV Biophysical techniques ✔ CD81|MHCII EV Enriched markers ✘ EV Negative markers ✘ EV Particle analysis Identified molecule protein Identification method Mass spectrometry PubMed ID 20458337     Organism Homo sapiens Experiment description MHC class II-associated proteins in B-cell exosomes and potential functional implications for exosome biogenesis - Sample 3 Authors Buschow SI, van Balkom BW, Aalberts M, Heck AJ, Wauben M, Stoorvogel W. Journal name ICB Publication year 2010 Sample B cells Sample name RN (HLA-DR15) Isolation/purification methods Differential centrifugation Sucrose density gradient Immunobeads (MHC Class II) Flotation density - Molecules identified in the study Protein Methods used in the study Mass spectrometry [FT-ICR] Western blotting

Protein-protein interactions for CD53

Protein Interactor ExoCarta ID Identification method PubMed Species 1 CD2 914 Invivo Homo sapiens 2 CD4 920 Invitro Homo sapiens 3 CD9 928 Affinity Capture-Western Homo sapiens View the network

Pathways in which CD53 is involved

No pathways found