Gene description for CD53
Gene name
CD53 molecule
Gene symbol
CD53
Other names/aliases
MOX44 TSPAN25
Species
Homo sapiens
Database cross references - CD53
ExoCarta
ExoCarta_963
Entrez Gene
963
HGNC
1686
MIM
151525
UniProt
P19397
CD53 identified in exosomes derived from the following tissue/cell type
B cells
9685355
B cells
20458337
B cells
20458337
Gene ontology annotations for CD53
Experiment description of studies that identified CD53 in exosomes
1
Experiment ID
16
ISEV standards
✔
EM
EV Biophysical techniques
✘
EV Cytosolic markers
✔
MHCII|CD86|LAMP1|LAMP2|CD63|CD81|CD82|ICAM1|TFRC
EV Membrane markers
✘
EV Negative markers
✘
EV Particle analysis
Identified molecule
protein
Identification method
Immunoelectron Microscopy
PubMed ID
9685355
Organism
Homo sapiens
Experiment description
Selective enrichment of tetraspan proteins on the internal vesicles of multivesicular endosomes and on exosomes secreted by human B-lymphocytes.
Authors
Escola JM, Kleijmeer MJ, Stoorvogel W, Griffith JM, Yoshie O, Geuze HJ
Journal name
JBC
Publication year
1998
Sample
B cells
Sample name
RN (HLA-DR15+)
Isolation/purification methods
Differential centrifugation Sucrose density gradient
Flotation density
1.12-1.18 g/mL
Molecules identified in the study
Protein
Methods used in the study
Western blotting Immunoelectron Microscopy
2
Experiment ID
79
ISEV standards
✘
EV Biophysical techniques
✘
EV Cytosolic markers
✔
CD81|MHCII
EV Membrane markers
✘
EV Negative markers
✘
EV Particle analysis
Identified molecule
protein
Identification method
Mass spectrometry
PubMed ID
20458337
Organism
Homo sapiens
Experiment description
MHC class II-associated proteins in B-cell exosomes and potential functional implications for exosome biogenesis - Sample 1
Authors
Buschow SI, van Balkom BW, Aalberts M, Heck AJ, Wauben M, Stoorvogel W.
Journal name
ICB
Publication year
2010
Sample
B cells
Sample name
RN (HLA-DR15)
Isolation/purification methods
Differential centrifugation Sucrose density gradient Immunobeads (MHC Class II)
Flotation density
-
Molecules identified in the study
Protein
Methods used in the study
Mass spectrometry [FT-ICR] Western blotting
3
Experiment ID
81
ISEV standards
✘
EV Biophysical techniques
✘
EV Cytosolic markers
✔
CD81|MHCII
EV Membrane markers
✘
EV Negative markers
✘
EV Particle analysis
Identified molecule
protein
Identification method
Mass spectrometry
PubMed ID
20458337
Organism
Homo sapiens
Experiment description
MHC class II-associated proteins in B-cell exosomes and potential functional implications for exosome biogenesis - Sample 3
Authors
Buschow SI, van Balkom BW, Aalberts M, Heck AJ, Wauben M, Stoorvogel W.
Journal name
ICB
Publication year
2010
Sample
B cells
Sample name
RN (HLA-DR15)
Isolation/purification methods
Differential centrifugation Sucrose density gradient Immunobeads (MHC Class II)
Flotation density
-
Molecules identified in the study
Protein
Methods used in the study
Mass spectrometry [FT-ICR] Western blotting
Protein-protein interactions for CD53
Protein Interactor
ExoCarta ID
Identification method
PubMed
Species
1
CD2
914
Invivo
Homo sapiens
2
CD4
920
Invitro
Homo sapiens
3
CD9
928
Affinity Capture-Western
Homo sapiens
View the network
image/svg+xml
Pathways in which CD53 is involved
No pathways found