Gene description for CD53
Gene name CD53 molecule
Gene symbol CD53
Other names/aliases MOX44
TSPAN25
Species Homo sapiens
 Database cross references - CD53
ExoCarta ExoCarta_963
Entrez Gene 963
HGNC 1686
MIM 151525
UniProt P19397  
 CD53 identified in exosomes derived from the following tissue/cell type
B cells 9685355    
B cells 20458337    
B cells 20458337    
 Gene ontology annotations for CD53
Molecular Function
    protein binding GO:0005515 IPI
Biological Process
    signal transduction GO:0007165 NAS
    positive regulation of myoblast fusion GO:1901741 ISS
Subcellular Localization
    cell surface GO:0009986 IEA
    immunological synapse GO:0001772 IDA
    extracellular exosome GO:0070062 IDA
    cell-cell junction GO:0005911 ISS
    plasma membrane GO:0005886 ISS
    integral component of membrane GO:0016021 IEA
 Experiment description of studies that identified CD53 in exosomes
1
Experiment ID 16
ISEV standards
EM
EV Biophysical techniques
EV Cytosolic markers
MHCII|CD86|LAMP1|LAMP2|CD63|CD81|CD82|ICAM1|TFRC
EV Membrane markers
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Immunoelectron Microscopy
PubMed ID 9685355    
Organism Homo sapiens
Experiment description Selective enrichment of tetraspan proteins on the internal vesicles of multivesicular endosomes and on exosomes secreted by human B-lymphocytes.
Authors Escola JM, Kleijmeer MJ, Stoorvogel W, Griffith JM, Yoshie O, Geuze HJ
Journal name JBC
Publication year 1998
Sample B cells
Sample name RN (HLA-DR15+)
Isolation/purification methods Differential centrifugation
Sucrose density gradient
Flotation density 1.12-1.18 g/mL
Molecules identified in the study Protein
Methods used in the study Western blotting
Immunoelectron Microscopy
2
Experiment ID 79
ISEV standards
EV Biophysical techniques
EV Cytosolic markers
CD81|MHCII
EV Membrane markers
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 20458337    
Organism Homo sapiens
Experiment description MHC class II-associated proteins in B-cell exosomes and potential functional implications for exosome biogenesis - Sample 1
Authors Buschow SI, van Balkom BW, Aalberts M, Heck AJ, Wauben M, Stoorvogel W.
Journal name ICB
Publication year 2010
Sample B cells
Sample name RN (HLA-DR15)
Isolation/purification methods Differential centrifugation
Sucrose density gradient
Immunobeads (MHC Class II)
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [FT-ICR]
Western blotting
3
Experiment ID 81
ISEV standards
EV Biophysical techniques
EV Cytosolic markers
CD81|MHCII
EV Membrane markers
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 20458337    
Organism Homo sapiens
Experiment description MHC class II-associated proteins in B-cell exosomes and potential functional implications for exosome biogenesis - Sample 3
Authors Buschow SI, van Balkom BW, Aalberts M, Heck AJ, Wauben M, Stoorvogel W.
Journal name ICB
Publication year 2010
Sample B cells
Sample name RN (HLA-DR15)
Isolation/purification methods Differential centrifugation
Sucrose density gradient
Immunobeads (MHC Class II)
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [FT-ICR]
Western blotting
 Protein-protein interactions for CD53
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 CD2 914
Invivo Homo sapiens
2 CD4 920
Invitro Homo sapiens
3 CD9 928
Affinity Capture-Western Homo sapiens
View the network image/svg+xml
 Pathways in which CD53 is involved
No pathways found





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