Gene description for CD53
Gene name CD53 molecule
Gene symbol CD53
Other names/aliases MOX44
TSPAN25
Species Homo sapiens
 Database cross references - CD53
ExoCarta ExoCarta_963
Vesiclepedia VP_963
Entrez Gene 963
HGNC 1686
MIM 151525
UniProt P19397  
 CD53 identified in exosomes derived from the following tissue/cell type
B cells 9685355    
B cells 20458337    
B cells 20458337    
 Gene ontology annotations for CD53
Molecular Function
    protein binding GO:0005515 IPI
    identical protein binding GO:0042802 IPI
    protein-membrane adaptor activity GO:0043495 IDA
Biological Process
    signal transduction GO:0007165 NAS
    receptor clustering GO:0043113 IDA
    positive regulation of myoblast fusion GO:1901741 ISS
Subcellular Localization
    immunological synapse GO:0001772 IDA
    plasma membrane GO:0005886 IBA
    plasma membrane GO:0005886 IDA
    plasma membrane GO:0005886 ISS
    plasma membrane GO:0005886 TAS
    cell-cell junction GO:0005911 ISS
    cell surface GO:0009986 IEA
    specific granule membrane GO:0035579 TAS
    synapse GO:0045202 IEA
    extracellular exosome GO:0070062 HDA
    tertiary granule membrane GO:0070821 TAS
 Experiment description of studies that identified CD53 in exosomes
1
Experiment ID 16
MISEV standards
✔
EM
Biophysical techniques
✔
MHCII|CD86|LAMP1|LAMP2|CD63|CD81|CD82|ICAM1|TFRC
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Immunoelectron Microscopy
PubMed ID 9685355    
Organism Homo sapiens
Experiment description Selective enrichment of tetraspan proteins on the internal vesicles of multivesicular endosomes and on exosomes secreted by human B-lymphocytes.
Authors "Escola JM, Kleijmeer MJ, Stoorvogel W, Griffith JM, Yoshie O, Geuze HJ"
Journal name JBC
Publication year 1998
Sample B cells
Sample name RN (HLA-DR15+)
Isolation/purification methods Differential centrifugation
Sucrose density gradient
Flotation density 1.12-1.18 g/mL
Molecules identified in the study Protein
Methods used in the study Western blotting
Immunoelectron Microscopy
2
Experiment ID 79
MISEV standards
✘ Biophysical techniques
✔
CD81|MHCII
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 20458337    
Organism Homo sapiens
Experiment description MHC class II-associated proteins in B-cell exosomes and potential functional implications for exosome biogenesis - Sample 1
Authors "Buschow SI, van Balkom BW, Aalberts M, Heck AJ, Wauben M, Stoorvogel W."
Journal name ICB
Publication year 2010
Sample B cells
Sample name RN (HLA-DR15)
Isolation/purification methods Differential centrifugation
Sucrose density gradient
Immunobeads (MHC Class II)
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [FT-ICR]
Western blotting
3
Experiment ID 81
MISEV standards
✘ Biophysical techniques
✔
CD81|MHCII
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 20458337    
Organism Homo sapiens
Experiment description MHC class II-associated proteins in B-cell exosomes and potential functional implications for exosome biogenesis - Sample 3
Authors "Buschow SI, van Balkom BW, Aalberts M, Heck AJ, Wauben M, Stoorvogel W."
Journal name ICB
Publication year 2010
Sample B cells
Sample name RN (HLA-DR15)
Isolation/purification methods Differential centrifugation
Sucrose density gradient
Immunobeads (MHC Class II)
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [FT-ICR]
Western blotting
 Protein-protein interactions for CD53
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 TGOLN2 10618
Affinity Capture-MS Homo sapiens
2 EFNA5  
Two-hybrid Homo sapiens
3 LOC100507537  
Two-hybrid Homo sapiens
4 CLCN7 1186
Two-hybrid Homo sapiens
5 SLC35A1 10559
Two-hybrid Homo sapiens
6 EMP1  
Two-hybrid Homo sapiens
7 CD53 963
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
8 TUSC5  
Two-hybrid Homo sapiens
9 ASGR2  
Two-hybrid Homo sapiens
10 AIG1  
Two-hybrid Homo sapiens
11 TMEM186  
Affinity Capture-MS Homo sapiens
12 VSTM1  
Two-hybrid Homo sapiens
13 MFSD8 256471
Affinity Capture-MS Homo sapiens
14 PLN  
Two-hybrid Homo sapiens
15 APOD 347
Two-hybrid Homo sapiens
16 MAL  
Two-hybrid Homo sapiens
17 RAP1BL 643752
Affinity Capture-MS Homo sapiens
18 RPRM  
Two-hybrid Homo sapiens
19 FAM171A2 284069
Affinity Capture-MS Homo sapiens
20 CD2 914
Affinity Capture-Western Homo sapiens
21 C2orf82  
Two-hybrid Homo sapiens
22 MUC1 4582
Two-hybrid Homo sapiens
23 TSPO2  
Two-hybrid Homo sapiens
24 LPAR3  
Two-hybrid Homo sapiens
25 GIMAP5  
Two-hybrid Homo sapiens
26 TMEM97 27346
Two-hybrid Homo sapiens
27 TMEM140  
Two-hybrid Homo sapiens
28 SMAGP  
Two-hybrid Homo sapiens
29 GPX8 493869
Two-hybrid Homo sapiens
30 CNIH2  
Two-hybrid Homo sapiens
31 KIR3DL3  
Two-hybrid Homo sapiens
32 CLDN7 1366
Two-hybrid Homo sapiens
33 CLEC7A  
Two-hybrid Homo sapiens
34 IGFBP5 3488
Two-hybrid Homo sapiens
35 IFITM3 10410
Two-hybrid Homo sapiens
36 PAM16  
Affinity Capture-MS Homo sapiens
37 KDSR 2531
Affinity Capture-MS Homo sapiens
38 CD37 951
Two-hybrid Homo sapiens
39 RAP1A 5906
Affinity Capture-MS Homo sapiens
40 CD81 975
Affinity Capture-Western Homo sapiens
41 ARL13B 200894
Two-hybrid Homo sapiens
42 CLDN19  
Two-hybrid Homo sapiens
43 ALG14  
Affinity Capture-MS Homo sapiens
44 C17orf62 79415
Two-hybrid Homo sapiens
45 ST6GAL2  
Two-hybrid Homo sapiens
46 TRAM1L1  
Two-hybrid Homo sapiens
47 TMEM60  
Two-hybrid Homo sapiens
48 GJA8  
Two-hybrid Homo sapiens
49 LAT 27040
Two-hybrid Homo sapiens
50 UPK1B 7348
Two-hybrid Homo sapiens
51 C14orf1 11161
Two-hybrid Homo sapiens
52 GPR108 56927
Two-hybrid Homo sapiens
53 TMEM128  
Two-hybrid Homo sapiens
54 VAMP3 9341
Affinity Capture-MS Homo sapiens
55 CD9 928
Affinity Capture-Western Homo sapiens
56 CREB3L1  
Two-hybrid Homo sapiens
57 LSMEM1  
Two-hybrid Homo sapiens
58 GPR152  
Two-hybrid Homo sapiens
59 TMEM86B  
Two-hybrid Homo sapiens
60 FAM134C 162427
Affinity Capture-MS Homo sapiens
61 TMEM229B  
Two-hybrid Homo sapiens
62 TMEM237 65062
Two-hybrid Homo sapiens
63 WRB 7485
Two-hybrid Homo sapiens
64 COL4A5  
Two-hybrid Homo sapiens
65 PSCA 8000
Two-hybrid Homo sapiens
66 PMP22  
Two-hybrid Homo sapiens
67 CTXN3  
Two-hybrid Homo sapiens
68 PLP2 5355
Two-hybrid Homo sapiens
69 NKG7  
Two-hybrid Homo sapiens
70 ECE2  
Two-hybrid Homo sapiens
71 CD82 3732
Affinity Capture-Western Homo sapiens
72 STX2 2054
Two-hybrid Homo sapiens
73 MS4A3  
Two-hybrid Homo sapiens
74 SMIM3  
Two-hybrid Homo sapiens
75 CD302 9936
Two-hybrid Homo sapiens
76 TMEM218  
Two-hybrid Homo sapiens
77 DNAJC19 131118
Affinity Capture-MS Homo sapiens
78 TMEM68  
Affinity Capture-MS Homo sapiens
79 TYW1  
Affinity Capture-MS Homo sapiens
80 FAM209A 200232
Two-hybrid Homo sapiens
81 BTN2A2  
Two-hybrid Homo sapiens
82 LHFPL5  
Two-hybrid Homo sapiens
83 ATP6V0C 527
Two-hybrid Homo sapiens
84 PRKCA 5578
Affinity Capture-Western Homo sapiens
85 VAMP5 10791
Two-hybrid Homo sapiens
86 CXCL16 58191
Two-hybrid Homo sapiens
87 SERTM1  
Two-hybrid Homo sapiens
88 GGT1 2678
Affinity Capture-Western Homo sapiens
89 UBIAD1 29914
Two-hybrid Homo sapiens
90 C1QL4  
Two-hybrid Homo sapiens
91 FAM210B  
Affinity Capture-MS Homo sapiens
View the network image/svg+xml
 Pathways in which CD53 is involved
PathwayEvidenceSource
Immune System TAS Reactome
Innate Immune System TAS Reactome
Neutrophil degranulation TAS Reactome





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